Sugarcane shoots and leaves consist of 38% cellulose, 30.6% hemicellulose and 12.8% lignin on dry solid （DS） basis and have the potential to serve as low cost feedstocks for ethanol production. The pretreatment and ...Sugarcane shoots and leaves consist of 38% cellulose, 30.6% hemicellulose and 12.8% lignin on dry solid （DS） basis and have the potential to serve as low cost feedstocks for ethanol production. The pretreatment and enzymatic hydrolysis conditions include particle size, alkali （NaOH）/dilute acid （H2SO4） pretreatment, chemical and substrate concentrations, temperature, autoclaving time for pretreatment, enzyme concentration, pH and temperature for hydrolysis varied were evaluated for conversion of sugarcane shoots and leaves cellulose and hemicellulose to reducing sugar. The optimum conditions were accomplished by using 14% w/v DS of 0-10 mm sugarcane shoots and leaves in particle size, pretreated with 1.5% w/v of dilute sulfuric acid at 121℃, 15 lbs/in2 for 15 min and enzymatic saccharification using 40 FPU/g DS cellulose at 50℃ and pH 5, After incubating at 160 rpm for 12 hrs, 59 g/L or 386,38 mg/g DS of reducing sugar and 50.69% saccharification were obtained.展开更多
Five elephant grass cultivars, Pennisetum purpureum, cv. Huanan （Huanan）, P. purpureum, cv. N51 （N51）, P. purpureum, cv. Sumu No. 2 （Sumu-2）, （ Penniseturn americanum x P. purpureum ） x P. purpureum cv. Guimu ...Five elephant grass cultivars, Pennisetum purpureum, cv. Huanan （Huanan）, P. purpureum, cv. N51 （N51）, P. purpureum, cv. Sumu No. 2 （Sumu-2）, （ Penniseturn americanum x P. purpureum ） x P. purpureum cv. Guimu No. 1 （Guimu-1） and P. americanum cv. Tift23A x P. purpureum cv. Tilt NS1 （Hybrid Pennisetum）, at three harvest stages were studied. With dilute sulfuric acid pretreatment followed by enzymatic hydrolysis, it is found that cel- lulose conversion of the five elephant grass cultivars harvested in August and September is higher than that har- vested in October. The cellulose conversion for elephant grass cultivars harvested in August and September follows an order of Hybrid Pennisetum 〉 Sumu-2 〉 Huanan 〉 Guimu-1 〉 N51. This may be explained by the fact that lignification is gradually strengthened with time, inhibiting degradation of cellulose and hemicellulose. Moreover, cellulose conversions of Hybrid Pennisetum, Sumu-2 and Huanan harvested in August and September are higher based on hierarchical clustering results.展开更多
文摘Sugarcane shoots and leaves consist of 38% cellulose, 30.6% hemicellulose and 12.8% lignin on dry solid （DS） basis and have the potential to serve as low cost feedstocks for ethanol production. The pretreatment and enzymatic hydrolysis conditions include particle size, alkali （NaOH）/dilute acid （H2SO4） pretreatment, chemical and substrate concentrations, temperature, autoclaving time for pretreatment, enzyme concentration, pH and temperature for hydrolysis varied were evaluated for conversion of sugarcane shoots and leaves cellulose and hemicellulose to reducing sugar. The optimum conditions were accomplished by using 14% w/v DS of 0-10 mm sugarcane shoots and leaves in particle size, pretreated with 1.5% w/v of dilute sulfuric acid at 121℃, 15 lbs/in2 for 15 min and enzymatic saccharification using 40 FPU/g DS cellulose at 50℃ and pH 5, After incubating at 160 rpm for 12 hrs, 59 g/L or 386,38 mg/g DS of reducing sugar and 50.69% saccharification were obtained.
基金Supported by Jiangsu Agriculture Science and Technology Innovation Fund(CX(11)4048)
文摘Five elephant grass cultivars, Pennisetum purpureum, cv. Huanan （Huanan）, P. purpureum, cv. N51 （N51）, P. purpureum, cv. Sumu No. 2 （Sumu-2）, （ Penniseturn americanum x P. purpureum ） x P. purpureum cv. Guimu No. 1 （Guimu-1） and P. americanum cv. Tift23A x P. purpureum cv. Tilt NS1 （Hybrid Pennisetum）, at three harvest stages were studied. With dilute sulfuric acid pretreatment followed by enzymatic hydrolysis, it is found that cel- lulose conversion of the five elephant grass cultivars harvested in August and September is higher than that har- vested in October. The cellulose conversion for elephant grass cultivars harvested in August and September follows an order of Hybrid Pennisetum 〉 Sumu-2 〉 Huanan 〉 Guimu-1 〉 N51. This may be explained by the fact that lignification is gradually strengthened with time, inhibiting degradation of cellulose and hemicellulose. Moreover, cellulose conversions of Hybrid Pennisetum, Sumu-2 and Huanan harvested in August and September are higher based on hierarchical clustering results.
