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Detection of Atrazine Residue in Food Samples by a Monoclonal Antibody-based Enzyme-linked Immunosorbent Assay 被引量:5
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作者 LV Zhi Qiang WANG Cai Hong +8 位作者 WANG Ting Ting CHEN Cui Cui WANG Ying NING Bao An LIU Ming LIU Jian Qing BAI Jia Lei PENG Yuan GAO Zhi Xian 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2013年第5期398-402,共5页
Atrazine(AT,2-chloro-4-ethylamino-6-isopropyl-amino-s-triazine)has been detected in ground water in several areas of the United States for many years,as well as in China,wherein the growth rate of its gross
关键词 Detection of Atrazine Residue in Food Samples by a Monoclonal Antibody-based Enzyme-linked immunosorbent assay elisa AT
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Determnation of ochratoxin A in grain by monoclonal antibody-based enzyme-linked immunosorbent assay
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作者 Yang Chuanhe Luo Xueyun +4 位作者 Liu Chang Li Wenyan Li Yiepeng Zhao Danyu Ji RongInstitute of Food Safety Control and inspection. Ministry of Public HealthBeijing 100021 . China 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 1994年第1期116-122,共7页
The simple rapid and sensitive enzyme-linked immunosorbent assay (ELISA) methods, di-rect and indirect ELISA, for quantitation of ochratoxin A in cereal had been developed by theutilization of monoclonal antibody on i... The simple rapid and sensitive enzyme-linked immunosorbent assay (ELISA) methods, di-rect and indirect ELISA, for quantitation of ochratoxin A in cereal had been developed by theutilization of monoclonal antibody on immunomicroplate. Direct FLIAS was found to be less timeconsuming than indirect ELISA. For direct FLISA, recovery of 1 -500 ppb OA added to wheat was78.9-100.0% and rice was 88.9- 120.0%. For indirect EI.IAS, recovery of 1-500 ppb OA addedto wheat was 79.0- 110.0% and rice was 82.0 120.0%. The minimal detection level for OA was Ippb. Analyses of 31 samples that caused humanintoxicant for OA showed that the ELISA resultsagreed wtll with those obtained by thin-layer chromatogrdphy. 展开更多
关键词 enzyme-linked immunosorbent assay (elisa) ochratoxin A monoclonal antibody cereal.
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Development of an Indirect Enzyme-Linked Immunosorbent Assay for Seromonitoring Contagious Bovine Pleuropneumonia Using Recombinant Lipoprotein LppQ of Mycoplasma mycoides subsp mycoides SC as Antigen
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作者 XIN Jiu-qing GAO Yun-long +2 位作者 LI Yuan WANG Yan-fan QIAN Ai-dong 《Agricultural Sciences in China》 CAS CSCD 2007年第1期100-107,共8页
Mycoplasma mycoides subsp mycoides SC (MmmSC) is the etiological agent of contagious bovine pleuropneumonia (CBPP). The lipoprotein LppQ encoded by lppQ gene is specific to MmmSC and is found in the type strain an... Mycoplasma mycoides subsp mycoides SC (MmmSC) is the etiological agent of contagious bovine pleuropneumonia (CBPP). The lipoprotein LppQ encoded by lppQ gene is specific to MmmSC and is found in the type strain and in field strains isolated in Europe, Africa, and Australia, as well as in vaccine strains. No serological cross-reactions were observed with the related mycoplasmas of the Mycoplasma mycoides cluster. The N-terminal domain of the mature lipoprotein LppQ is hydrophilic, and it induces a strong, specific, early, and persistent immune response in naturally and experimentally infected animals. Mycoplasma-specific TGA (Trp) codons are utilized as stop codons in most other organisms. The lppQ N-terminal fragment from MmmSC HVRI X strain, the Chinese strain for CF antigen production, was mutated with one-step overlapping extension PCR. Sequence analysis confirmed the successful mutation from A to G in codon 198 in the lppQ gene. The fragment containing the mutation site was subcloned into the pET32a expression vector. The recombinant protein with molecular weight of 42 kDa was purified using the Ni-NTA His.Bind purification kit, with a purity of up to 95%. Western blot indicated that the standard positive serum of CBPP could react with the recombinant protein. The purified protein was diluted to 0.35 μg mL^-1, and coated to microtiter enzyme-linked immunosorbent assay (ELISA) plates. Indirect ELISA reaction conditions were optimized. The value of P/N was determined to be 4.8 (0.934/0.193), the sensitivity to be 95.8% (46/48), and the specificity to be 98.9% (161/163). 3 817 cattle serum samples from three different provinces were detected by the indirect ELISA and CFT. The Kappa value is 0.63, which is middle or high agreement between the two methods. 展开更多
关键词 contagious bovine pleuropneumonia (CBPP) lipoprotein LppQ MUTAGENESIS indirect enzyme-linked immunosorbent assay elisa
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A Comparison of Enzyme-Linked Immunosorbent Assay versus Multiplex Methodology Using an <i>in Vitro</i>Model of Pulmonary Hypertension and Inflammation
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作者 Yan Zhu Deepthi Alapati +3 位作者 Joanna Costa Victoria L. Maduskuie Paul T. Fawcett Thomas H. Shaffer 《Journal of Biomedical Science and Engineering》 2014年第7期419-426,共8页
Enzyme-linked immunosorbent assay (ELISA) is the most widely used method for measuring a single cytokine. Recent developments in cytokine quantification such as multiple arrays measure multiple cytokines simultaneousl... Enzyme-linked immunosorbent assay (ELISA) is the most widely used method for measuring a single cytokine. Recent developments in cytokine quantification such as multiple arrays measure multiple cytokines simultaneously. Although good correlations between ELISA and multiplex methods have been observed, side by side comparisons are limited. In the present study we hypothesized that ELISA and Luminex techniques are comparable in detecting cytokines in culture medium when pulmonary artery smooth muscle cells (PASMC) are exposed to stress. Primary human PASMC were cultured in modular chambers and exposed to 21% FiO2 and peak inspiratory and positive end expiratory pressure of 24 and 8 cmH2O respectively, and 95% FiO2. At 24 hours, culture medium was collected and assayed for interleukin-6 (IL-6) and IL-8 by quantitative ELISA and by Human Cytokine 25-Plex Panel using a Luminex 200 analyzer. A comparative analysis of agreement between our ELISA and Luminex data was detailed for control and stress conditions using the Bland-Altman plot analysis. Each assay resulted in comparable increased (p < 0.001) levels of IL-6 and IL-8 as compared to control in response to oxidative and biophysical stress. The Bland-Altman analysis demonstrated that 95% of the differences between ELISA and Luminex values were within ±1.96 SD from the mean difference indicated by the 95% limits of agreement for the measurements of IL-6 and IL-8. There was no systematic bias as a function of inflammation level. We conclude that in this cell culture model, ELISA and Luminex are comparable in detecting the levels of IL-6 and IL-8 in the culture medium. If measurements of multiple cytokines are demanded and the amount of sample is limited, Luminex multi-analyte profiling technology is accurate and sensitive. 展开更多
关键词 Enzyme-Linked immunosorbent assay (elisa) LUMINEX Pulmonary Artery Smooth Muscle Cells (PASMC) INFLAMMATION Bland-Altman PLOT Analysis
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SERODIAGNOSIS OF CLONORCHIASIS BY ENZYME—LINKED IMMUNOSORBENT ASSAY WITH HRP—SPA
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作者 谷宗藩 王尊哲 +2 位作者 崔巍 王士谔 黄红 《潍坊医学院学报》 1985年第2期146-151,共6页
In thes paper the authors used the Horseradish peroxidase labelledstaphylococcal protein A(HRP—SPA)in ELISA,for the detection of Clo-norchis sinensis infection.Serum tests were made on 116 confirmed cases ofclonorchi... In thes paper the authors used the Horseradish peroxidase labelledstaphylococcal protein A(HRP—SPA)in ELISA,for the detection of Clo-norchis sinensis infection.Serum tests were made on 116 confirmed cases ofclonorchiasis,103(88.8%)of them showed positive,while only 6(4.4%)werepositive among 138 healthy people.Samples were collected on filter paperstrips,111(95.7%)cases were positive among 116 comfirmed cases tested,but only 2(1.5%)were positive out of 138 healthy persons.The resultswere similar to those obtained by sheep antihuman IgG.Animal experimentalso showed that the SPA—ELISA can be used for the diagnosis ofclonorchiasis.In an endemic area,stool egg positive rate was 8.8%(62/703).whenchecked with SPA—ELISA,the rate of conformity in both filter paperstrips and stool examinations was 90.3(56/62).Among 641 serum testsfrom individuals negative in stool examinations,only 35(5.5%)reactedpositively.The authors suggested—that SPA—ELISA with soluble Clo-norchis antigens could be used in a large scale seroepidemiological surveyin endemic areas. 展开更多
关键词 LINKED immunosorbent assay WITH HRP elisa SERODIAGNOSIS OF CLONORCHIASIS BY ENZYME SPA
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新霉素ELISA检测方法的建立 被引量:9
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作者 刘沙洲 桑小雪 +2 位作者 欧阳华学 雷绍荣 白林含 《食品科学》 EI CAS CSCD 北大核心 2011年第14期227-231,共5页
目的:比较直接和间接竞争酶联免疫法(enzyme linked immunosorbent assay,ELISA)的优缺点,建立新霉素残留ELISA检测方法。方法:利用自制的新霉素多克隆抗体,采用直接竞争和间接竞争ELISA方法检测新霉素残留,并比较两种方法的优缺点。结... 目的:比较直接和间接竞争酶联免疫法(enzyme linked immunosorbent assay,ELISA)的优缺点,建立新霉素残留ELISA检测方法。方法:利用自制的新霉素多克隆抗体,采用直接竞争和间接竞争ELISA方法检测新霉素残留,并比较两种方法的优缺点。结果:新霉素抗血清和庆大霉素的交叉反应率为2.04%,和卡那霉素的交叉反应率为0.02%,和氨苄青霉素、红霉素、四环素的交叉反应率均小于0.01%。初步测试新霉素间接竞争ELISA法的准确性和回收率。板内误差小于4%,板间误差小于11%,回收率为135.5%~191.3%。直接竞争和间接竞争ELISA方法的检测极限分别为28.58ng/mL和51.74ng/mL,达到了国家对新霉素规定的500μg/kg MRL检测限。结论:建立了直接竞争和间接ELISA吸附检测方法,条件优化更成功的间接竞争ELISA可用于开发新霉素检测试剂盒。 展开更多
关键词 新霉素 多克隆抗体 竞争酶联免疫法(enzyme linked immunosorbent assay elisa) 方法建立
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Concerns arise: wheat allergy risk in pre-packaged food products from China
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作者 Wenfeng Liu Jian Wang +5 位作者 Zhongliang Wang Fangfang Min Yong Wu Juanli Yuan Jinyan Gao Hongbing Chen 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第6期3139-3149,共11页
Understanding and monitoring the cross-contamination of food allergens is crucial for safeguarding public health and ensuring food safety.Food allergen risk assessment,derived from classical toxicological principles,c... Understanding and monitoring the cross-contamination of food allergens is crucial for safeguarding public health and ensuring food safety.Food allergen risk assessment,derived from classical toxicological principles,can identify and quantify the risk of allergies.This study aimed to investigate the risk of wheat allergic reactions to prepackaged foods from China through the utilization of food allergen risk assessment.A total of 575 products have been surveyed,wheat/gluten,milk and egg were major allergens labelled on products.According to voluntary incidental trace allergen labelling 3.0(VITAL®3.0)program,the number of products belonged to Action Level 2 were 303.Integration of precautionary allergen labeling(PAL)analysis indicated that 9.57%products would pose a potential risk to wheat allergic individuals.The probabilistic risk assessment results suggest that 7984 allergic reactions may arise among wheat-allergic consumers during 10000 eating occasions due to the consumption of pre-packaged food products with incorrect wheat-related allergen labelling.This study demonstrated that a risk assessment-based approach can support the guidance of allergen labelling and management of food allergen for pre-packaged food products,providing protection for allergic individuals in food consumption and for food manufacturers in food production and trade. 展开更多
关键词 Food allergens Allergen labelling Pre-packaged food Enzyme linked immunosorbent assay(elisa) Voluntary incidental trace allergen labelling (VITAL) Quantitative risk assessment
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Establishment of enzyme-linked immunosorbent assay for beef and lamb contents in cooked meat
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作者 Yujing Li Jingjing Liu +6 位作者 Sufang Fan Li Zhao Jing Zhang Erjing Zhang Ziran Li Yan Zhang Chunsheng Li 《Journal of Future Foods》 2024年第1期91-96,共6页
In this study,an enzyme 1linked immunosorbent assay(ELISA)was established to detect beef and 1amb components,and its performance was tested.Double-antibody sandwich ELISA was adopted and determined a coating concentra... In this study,an enzyme 1linked immunosorbent assay(ELISA)was established to detect beef and 1amb components,and its performance was tested.Double-antibody sandwich ELISA was adopted and determined a coating concentration of capture antibody 3G5 of 1:4000,a working concentration of enzyme-labeled antibody 2E7-horseradish peroxidase(HRP)of 1:1000,a sample incubation time of 60 min and a detection antibody reaction time of 60 min.The specificity,sensitivity,repeatability and stability of this assay were detemmined.The limit of detection for beef and 1amb skeleta1 muscle troponin I was 45 mg/kg,the inter-assay and intra-assay recovery rates ranged from 80.4%to 115.7%,the coefficients of variation were below 13.6%,and the cIoss reaction rates of the tissue components of chicken,duck and fish were below 13.4%.The sandwich ELISA method established in this study is stable and has high accuracy.The test results were consistent with the polymerase chain reaction(PCR)method at 50 and 100 g/kg-Therefore,this ELISA method can be used to quantitatively detect beef and 1amb components in meat products. 展开更多
关键词 Double antibody sandwich enzyme-1inked immunosorbent assay(elisa) Beef components Lamb components
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Immunoregulatory Effect and Mechanism of Epigallocatechin-3-Gallate in A Mouse Oral Cancer Model
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作者 Yizhen Li Siyi Huang +4 位作者 Yanzi Ling Liyan Fu Ruyue Zheng Xinwei Duan Yueji Luo 《Proceedings of Anticancer Research》 2024年第5期82-88,共7页
Objective:This investigation delineates the anti-cancer potency of epigallocatechin-3-gallate(EGCG)in an oral cancer mouse model,with a focus on its effect on T-cell activation.Methods:An oral cancer model was establi... Objective:This investigation delineates the anti-cancer potency of epigallocatechin-3-gallate(EGCG)in an oral cancer mouse model,with a focus on its effect on T-cell activation.Methods:An oral cancer model was established in male Balb/c mice using 4-nitroquinoline 1-oxide(4-NQO).The mice were systematically grouped and administered graded concentrations of EGCG.Key parameters such as body weight,hydration levels,tumor volume,and mass were meticulously tracked.T-cell activity and cytokine expression profiles,focusing on interleukin-2(IL-2),interferon-gamma(IFN-γ),and tumor necrosis factor-alpha(TNF-α),were quantified using ELISA.A comprehensive statistical evaluation included one-way ANOVA,Tukey’s HSD multiple comparison test,and the Kruskal-Wallis non-parametric assessment.Results:EGCG-administered cohorts exhibited a pronounced reduction in tumor size and mass,with the high-dose group showing the greatest efficacy.ELISA findings corroborated a significant increase in T-cell activity and concomitant upregulation of key cytokines,including IL-2,IFN-γ,and TNF-α(P<0.05).Conclusion:This investigation confirms the tumor-suppressive efficacy of EGCG in a murine oral squamous cell carcinoma model.The therapeutic effects of EGCG are mediated through T-cell activation and the upregulation of pivotal cytokine expression,highlighting its potential immunomodulatory role in oral cancer treatment. 展开更多
关键词 Epigallocatechin-3-gallate(EGCG) Oral squamous cell carcinoma(OSCC) 4-nitroquinoline 1-oxide(4-NQO) Peripheral blood mononuclear cell(PBMC) Enzyme-linked immunosorbent assay(elisa)
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Sandwich ELISA for detecting urinary Survivin in bladder cancer 被引量:4
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作者 Xuefeng Li Yaming Wang +1 位作者 Jianjun Xu Qingyun Zhang 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2013年第4期375-381,共7页
Objective: Survivin as a tumor marker in the diagnosis of bladder cancer has not been completely confirmed yet and there are few reports about using Survivin enzyme-linked immunosorbent assay (ELISA) kit to detect ... Objective: Survivin as a tumor marker in the diagnosis of bladder cancer has not been completely confirmed yet and there are few reports about using Survivin enzyme-linked immunosorbent assay (ELISA) kit to detect the urine of bladder cancer patients. This study aimed to develop a Survivin ELISA and validate its value in the detection of bladder cancer. Methods: Through square matrix titration, different combinations of coating antibody and detecting antibody, a Survivin ELISA was constructed. This assay was evaluated according to intra-assay precision, inter-assay precision and minimum detectable dose (MDD). Survivin levels were detected and analyzed in 102 bladder cancer patients and 102 healthy people by established ELISA. Then cutoff value was defined according to the analysis of receiver operating characteristic (ROC) curve. The sensitivity and specificity of detection were calculated on the basis of cutoff value to diagnose bladder cancer patients. Furthermore, the value of Survivin expression detected by ELISA among different clinicopathological characteristics of patients was also compared. Results: Through optimization of different conditions, intra-assay precision was 8.39%, inter-assay precision 8.57% and MDD 0.0625 ng/mL in this assay. When the optical density at 450 nm (OD 450 ) was 0.09, it could get the optimized diagnostic cutoff value. According to this value, the sensitivity and specificity of diagnosis in bladder cancer patients were 70.6% and 89.2%, respectively. The associations between patients' clinical variables and OD 450 were not significant except tumor numbers in patients. Conclusions: This experiment has preliminarily developed a Survivin ELISA and confirmed Survivin as a biomarker which owned a practical and significant value in the diagnosis of bladder cancer. 展开更多
关键词 SURVIVIN bladder cancer enzyme-linked immunosorbent assay elisa tumor marker DIAGNOSIS
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液相色谱-质谱联用法(LC-MS/MS)和酶联免疫法(ELISA)对体内25(OH)D3水平的测定 被引量:9
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作者 毛旭东 吴彦 +4 位作者 盛宏光 刘志文 王春平 李水军 王洪复 《中国骨质疏松杂志》 CAS CSCD 北大核心 2013年第6期584-586,共3页
目的同时用液相色谱-质谱联用法(LC-MS/MS)和酶联免疫法(ELISA)检测患者体内25(OH)D3水平,分析两者结果的差异。方法随机选取50例住院患者,对同一血清样本分别用LC-MS/MS法和ELISA法测定25(OH)D3水平,同时用LC-MS/MS法测定25(OH)D2的水... 目的同时用液相色谱-质谱联用法(LC-MS/MS)和酶联免疫法(ELISA)检测患者体内25(OH)D3水平,分析两者结果的差异。方法随机选取50例住院患者,对同一血清样本分别用LC-MS/MS法和ELISA法测定25(OH)D3水平,同时用LC-MS/MS法测定25(OH)D2的水平。结果 LC-MS/MS法测定的维生素D3的均数为14.99±6.51 ng/mL,酶联免疫法测定的均数为20.91±9.70 ng/mL,两者的相关系数为0.725(P<0.01),线性相关方程为维生素D3(LC-MS/MS法)=4.829+0.486×维生素D3(ELISA法)。LC-MS/MS法组25(OH)D3浓度高于20 ng/mL的比例17%,酶联免疫法组为52%,LC-MS/MS法组的25(OH)D2和25(OH)D3总浓度高于20 ng/mL的为24%。25(OH)D2占25(OH)D总量的8.4%。结论 LC-MS/MS法测定的维生素D3的数值明显低于ELISA法,两者正相关性较高,可经方程互换。酶联免疫法低估了体内维生素D的缺乏,检测25(OH)D3的同时需测定25(OH)D2浓度。 展开更多
关键词 液相色谱-质谱联用(LC-MS MS) 酶联免疫法(Enzyme-linked immunosorbent assay elisa) 25(OH)D3 25 (OH)D2
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Simple and sensitive determination of sparfloxacin in pharmaceuticals and biological samples by immunoassay 被引量:2
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作者 Hua-Jin Zenga,Ran Yangb,Bing Liub,Li-Fang Leib,Jian-Jun Lib,Ling-Bo Qub,c,n aSchool of Pharmaceutical Sciences,Zhengzhou University,Zhengzhou 450001,China bDepartment of Chemistry,Zhengzhou University,Zhengzhou 450001,China cSchool of Chemistry & Chemical Engineering,Henan University of Technology,Zhengzhou 450001,China 《Journal of Pharmaceutical Analysis》 SCIE CAS 2012年第3期214-219,共6页
Plasma quinolone concentrations are not routinely measured in clinical practice.However,in order to optimize quinolone treatment,monitoring of plasma concentrations could sometimes be useful particularly in critically... Plasma quinolone concentrations are not routinely measured in clinical practice.However,in order to optimize quinolone treatment,monitoring of plasma concentrations could sometimes be useful particularly in critically ill patients.In this study,anti-sparfloxacin antibody was obtained by immunizing rabbits with sparfloxacin conjugated with bovine serum albumin using isobutyl chloroformate method.After the assay procedure was optimized,the standard curve of sparfloxacin was established.The practical measuring range of the competitive ELISA extended from 5 ng/mL to 2 mg/mL.The recovery rates and coefficients of variation for rat plasma,urine and tissues were 87.7-106.2% and 4.8-15.3%,respectively.To demonstrate the potential of the ELISA,a preliminary pharmacokinetics and tissue distribution study of sparfloxacin in rats and quantitative analysis of sparfloxacin in several pharmaceuticals were performed and compared with high-performance liquid chromatography(HPLC).The experimental data indicated that the proposed method would be a valuable tool in therapeutic drug monitoring(TDM) for sparfloxacin. 展开更多
关键词 SPARFLOXACIN Enzyme-linked immunosorbent assay(elisa) Biological samples PHARMACOKINETICS Tissue distribution
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Development and evaluation of immunoassay for zeranol in bovine urine 被引量:2
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作者 LIU Yuan ZHANG Cun-zhen +5 位作者 YU Xiang-yang ZHANG Zhi-yong ZHANG Xiao LIU Rong-rong LIU Xian-jin GONG Zhen-ming 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2007年第12期900-905,共6页
A high affinity polyclonal antibody-based enzyme linked immunosorbent assay (ELISA) was developed for the quantification of zeranol in bovine urine. On the basis of urine matrix studies, the optimized dilution facto... A high affinity polyclonal antibody-based enzyme linked immunosorbent assay (ELISA) was developed for the quantification of zeranol in bovine urine. On the basis of urine matrix studies, the optimized dilution factors producing insignificant matrix interference were selected as 1:5 in pretreatment. In the improved ELISA, the linear response range was between 0.02 and 1 μg/ml, and the detection limit was 0.02 μg/ml for the assay. The overall recoveries and the coefficients of variation (CVs) were in the range of 82%-127% and 3.5%-8.8%, respectively. Thirty-six bovine urine samples spiked with zeranol (ranging from 0.2 to 10 μg/ml) were detected by the ELISA and liquid chromatography (LC) method, and good correlations were obtained between the two methods (R^2=0.9643). We conclude that this improved ELISA is suitable tool for a mass zeranol screening and can be an altemative for the conventional LC method for zeranol in bovine urine. 展开更多
关键词 ZERANOL Enzyme linked immunosorbent assay elisa Bovine urine
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Development of a sandwich ELISA for the detection of bovine herpesvirus type 1
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作者 Shanaz Bashir Rashmi Singh +1 位作者 Barkha Sharma Sharad K Yadav 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2011年第5期363-366,共4页
Objective:To develop a standard enzyme-linked immunosorbent assay(ELJSA) for the detection of bovine herpesvirus type 1(BHV-1).Methods:The assay was based on hyperimmune rabbit and guinea pig antisera raised again... Objective:To develop a standard enzyme-linked immunosorbent assay(ELJSA) for the detection of bovine herpesvirus type 1(BHV-1).Methods:The assay was based on hyperimmune rabbit and guinea pig antisera raised against purified BHV-1.Polyethylene glycol precipitation and sucrose density gradient methods were adopted for viral concentration and purification.Antisera were raised using Freund’s adjuvant followed by extraction of IgG of high purity.Results: Optimum antisera dilutions as determined by titrations were chosen as 14 000,whereas the conjugate was used at 1:2 000 dilution.Using 95 clinical specimens,the ELISA test showed a sensitivity and specificity of 91.90%and 93.10%,respectively when compared to PCR.The cutoff value was fixed at 0.15<sub>490</sub>) and a P/N ratio of】1.30 indicated a significant positive reaction. Conclusions:The results have demonstrated that this ELISA could efficiently detect BHV-1 and can be used as an important diagnostic tool. 展开更多
关键词 BOVINE HERPESVIRUS TYPE 1 ANTISERA Enzyme-linked immunosorbent assay (elisa)
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三种酶联免疫吸附法筛选“渗漏”型SCID小鼠的比较 被引量:7
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作者 李艺 崔恒 +1 位作者 叶雪 姚煜 《解剖学报》 CAS CSCD 北大核心 2002年第1期111-112,共2页
目的 选择简单的方法筛选“渗漏”型SCID小鼠。 方法 采用 3种酶联免疫吸附法 (双抗夹心ELISA、双抗夹心间接ELISA及生物素 亲和素夹心ELISA)测定SCID小鼠血清中IgG含量。 结果 生物素 亲和素夹心ELISA法灵敏度最高 ,用此法共检测... 目的 选择简单的方法筛选“渗漏”型SCID小鼠。 方法 采用 3种酶联免疫吸附法 (双抗夹心ELISA、双抗夹心间接ELISA及生物素 亲和素夹心ELISA)测定SCID小鼠血清中IgG含量。 结果 生物素 亲和素夹心ELISA法灵敏度最高 ,用此法共检测了 4 1只 6~ 8周龄小鼠 ,其中 4 0只的IgG含量为 0 2 4± 0 16mg L ;只有 1只小鼠IgG含量为 2 3mg L ,渗漏比例为 2 4 %。 结论 用生物素 亲和素夹心ELISA法对SCID小鼠“渗漏”进行初筛 ,是简便、敏感。 展开更多
关键词 渗漏 酶联免疫吸附法 SCID小鼠
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重症大疱性类天疱疮患者血清抗体变化规律与病情相关性的研究 被引量:3
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作者 赵英 王宇 +2 位作者 安蔚 陈蕾 王敬 《中国急救医学》 CAS CSCD 北大核心 2014年第4期342-344,共3页
目的:研究重症大疱性类天疱疮( bullous pemphigoid , BP )患者血清中抗体BP180NC16a的酶联免疫吸附试验(ELISA)指数变化情况,观察其与病情变化的相关性,并分析用于病情监测和指导治疗的临床意义。方法对12例皮损面积>50%的... 目的:研究重症大疱性类天疱疮( bullous pemphigoid , BP )患者血清中抗体BP180NC16a的酶联免疫吸附试验(ELISA)指数变化情况,观察其与病情变化的相关性,并分析用于病情监测和指导治疗的临床意义。方法对12例皮损面积>50%的重症大疱性类天疱疮患者血清抗体BP180 NC16 a水平在不同时期进行监测及评分,并分析之间的关系。结果12例患者,平均年龄65岁,皮损面积均大于全身体表面积的50%以上。皮疹主要表现为疱壁紧张的大疱、水疱,部分有口腔黏膜损害。患者皮损面积和病情评分与血清抗体BP180NC16a-ELISA指数具有显著性关联(P<0.05),患者疾病活动期和临床缓解期抗体BP180NC16a-ELISA指数几乎与病情呈平行变化,并且该指数可以预测病情,从而指导治疗。结论重症大疱性类天疱疮多为老年患者,病情危重,在发病早期不易诊断,因而延误治疗导致死亡。血清中抗体BP180 NC16 a-ELISA 指数可反映疾病的活动程度,用于病情监测,为治疗时根据个体差异选用适量的糖皮质激素快速控制病情提供了有利的实验室证据。 展开更多
关键词 重症大疱性类天疱疮( BP) 酶联免疫吸附试验( elisa) 病情监测 ENZYME linked immunosorbent assay ( elisa)
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食品中丙烯酰胺检测方法的研究进展 被引量:10
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作者 李娜 许翎婕 +1 位作者 李清明 郭时印 《食品研究与开发》 CAS 北大核心 2018年第9期213-219,共7页
丙烯酰胺是在食品高温加工过程中产生的小分子有机化合物,具有致癌性。从样品提取、衍生化、净化、富集等前处理过程以及对检测器的选择出发,总结国内外近年来用于检测丙烯酰胺的方法,如从传统的气相色谱、液相色谱及其联用技术,到新兴... 丙烯酰胺是在食品高温加工过程中产生的小分子有机化合物,具有致癌性。从样品提取、衍生化、净化、富集等前处理过程以及对检测器的选择出发,总结国内外近年来用于检测丙烯酰胺的方法,如从传统的气相色谱、液相色谱及其联用技术,到新兴开发的分子印迹技术、酶联免疫吸附和生物传感器等新检测技术。并根据其适用范围和操作条件,对各分析方法的优点和不足进行讲述,最后对将来丙烯酰胺检测方法发展新思路提供策略和依据。 展开更多
关键词 丙烯酰胺 检测技术 固相微萃取 分子印迹技术(molecular IMPRINTING technology MIT) 酶联免疫吸附法(enzyme-linked immunosorbent assay elisa)
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食管鳞癌患者肿瘤组织及血清中E-钙黏蛋白表达的临床意义 被引量:4
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作者 张军 何赢 +6 位作者 王书奎 黄文斌 陈兴国 何帮顺 赵有财 王劲松 张国新 《中南大学学报(医学版)》 CAS CSCD 北大核心 2012年第3期228-232,共5页
目的:检测食管鳞癌患者肿瘤组织及血清中E-钙黏蛋白(E-cadherin,E-cad)的表达并探讨其临床意义。方法:采用免疫组织化学法对48例食管鳞癌、23例糜烂性食管炎、24例正常食管黏膜组织中E-cad的表达进行检测;同时采用酶联免疫吸附法(ELISA... 目的:检测食管鳞癌患者肿瘤组织及血清中E-钙黏蛋白(E-cadherin,E-cad)的表达并探讨其临床意义。方法:采用免疫组织化学法对48例食管鳞癌、23例糜烂性食管炎、24例正常食管黏膜组织中E-cad的表达进行检测;同时采用酶联免疫吸附法(ELISA)对上述研究对象血清中可溶性E-cad(sE-cad)的表达进行检测,并且收集完整的临床病理资料。结果:E-cad表达水平在食管鳞癌组织中明显降低,与正常食管黏膜上皮组织及糜烂性食管炎黏膜上皮组织相比,差异有统计学意义(P<0.05)。不同浸润深度、有无淋巴结转移及不同分化程度的食管鳞癌,其癌组织中E-cad的表达水平差异有统计学意义(P<0.05)。血清sE-cad在食管鳞癌组表达水平明显高于正常食管组及糜烂性食管炎组(P<0.05)。血清sE-cad水平与食管鳞癌的临床病理特征无关。食管鳞癌患者肿瘤组织中E-cad高表达者与低表达者相比,血清sE-cad水平差异无统计学意义(P=0.134)。结论:E-cad可作为食管鳞癌诊断及预后判断的辅助指标。血清sE-cad可作为食管鳞癌诊断筛查的血清学辅助指标。 展开更多
关键词 E-钙黏蛋白 食管鳞癌 免疫组织化学 酶联免疫吸附
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Expression of Bt Protein in Transgenic Pest-resistant Rice 被引量:3
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作者 于志晶 蔡勤安 +1 位作者 林秀峰 马瑞 《Agricultural Science & Technology》 CAS 2012年第3期489-491,共3页
[Objective] The aim of this study was to study on expression of Bt protein in transgenic pest-resistant rice. [Method] Enzyme-linked immunosorbent assay (ELISA) was used to measure Bt protein expression in different... [Objective] The aim of this study was to study on expression of Bt protein in transgenic pest-resistant rice. [Method] Enzyme-linked immunosorbent assay (ELISA) was used to measure Bt protein expression in different tissues of transgenic pest-resistant rice at same growth stage. [Result] Absolute content of Bt protein from high to low was as follows: leaves 〉 immature seeds and glumes 〉 roots 〉 stems in different tissues of transgenic rice in grain-filling stage; Bt protein content of trans- genic rice changed a little in different growth stages (including tillering stage, booting stage, and grain-filling stage); in general, its level declined a little in later growth stage, but the resistibility would not be influenced significantly. [Conclusion] The ex- periment is significant for pest prevention and transgenic rice breeding. 展开更多
关键词 Enzyme-linked immunosorbent assay elisa Transgenic pest-resistant rice Bt protein
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乙型肝炎表面抗原不同检测方法的优势比较分析 被引量:6
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作者 侯娟 陈伟金 黄宏黎 《当代医学》 2014年第12期23-24,共2页
目的:比较化学发光微粒子免疫分析法(CMIA)和酶联免疫吸附法(ELISA)用于乙型肝炎(乙肝)表面抗原检测的优势效果。方法选取2011年3月~2013年4月采用ELISA法与CMIA法对386例患者进行了乙肝表面抗原检测的对比研究。结果 CMIA法检... 目的:比较化学发光微粒子免疫分析法(CMIA)和酶联免疫吸附法(ELISA)用于乙型肝炎(乙肝)表面抗原检测的优势效果。方法选取2011年3月~2013年4月采用ELISA法与CMIA法对386例患者进行了乙肝表面抗原检测的对比研究。结果 CMIA法检测乙肝表面抗原的阳性率为51.3%,ELISA法阳性率为43.5%,CMIA法阳性率明显更高,与ELISA法比较差异有统计学意义(P〈0.05);CMIA法的灵敏度与特异性均明显高于ELISA法(P〈0.05)。结论与ELISA法比较,CMIA法对乙肝表面抗原检测在阳性率、灵敏度以及特异性等方面均存在有比较明显的优势,故建议将CMIA法作为临床检测乙肝表面抗原的首选方法而推广应用。 展开更多
关键词 乙肝表面抗原 化学发光微粒子免疫分析法 酶联免疫吸附 对比研究 Chemiluminescent MICROPARTICLE IMMUNO assay(CMIA) Enzyme-Linked immunosorbent assay (elisa)
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