To investigate the dynamic changes of eosinophilic accumulation and apoptosis in the airway of asthmatic guinea pigs and explore the significance of eosinophilic apoptosis in eosinophilia. Methods: we made a detailed ...To investigate the dynamic changes of eosinophilic accumulation and apoptosis in the airway of asthmatic guinea pigs and explore the significance of eosinophilic apoptosis in eosinophilia. Methods: we made a detailed examination of the histology, cytology and apoptosis in the airway up to 7 d after antigen challenge. Apoptosis was assessed with in situ tailing. Results: The findings were as follows: ① After antigen challenge, eosinophil infiltration in the bronchi was the most serious from & to 24 h and then declined. ② Eosinophilia was remarkable in bronchoalveolar lavage fluid (BALF) and bronchial tissue at 8 h,peaked during 24 to 72 h and persisted for 7 days at least. ③ Eosinophilia was accompanied by epithelia damage and subepithelial edema. ④ The apoptosis rate of eosinophils in BALF was significantly lower in the asthmatic guinea pigs than in the normal controls (P<0. 05 ). Conclusion: These findings suggest that the apoptosis of eosinophils in asthmatic guinea pigs is inhibited, which may contribute to protracted course of eosinophilia in the airway.展开更多
文摘To investigate the dynamic changes of eosinophilic accumulation and apoptosis in the airway of asthmatic guinea pigs and explore the significance of eosinophilic apoptosis in eosinophilia. Methods: we made a detailed examination of the histology, cytology and apoptosis in the airway up to 7 d after antigen challenge. Apoptosis was assessed with in situ tailing. Results: The findings were as follows: ① After antigen challenge, eosinophil infiltration in the bronchi was the most serious from & to 24 h and then declined. ② Eosinophilia was remarkable in bronchoalveolar lavage fluid (BALF) and bronchial tissue at 8 h,peaked during 24 to 72 h and persisted for 7 days at least. ③ Eosinophilia was accompanied by epithelia damage and subepithelial edema. ④ The apoptosis rate of eosinophils in BALF was significantly lower in the asthmatic guinea pigs than in the normal controls (P<0. 05 ). Conclusion: These findings suggest that the apoptosis of eosinophils in asthmatic guinea pigs is inhibited, which may contribute to protracted course of eosinophilia in the airway.