Identification and enumeration of both dendritic Ia^+ epi-dermal cells (Ia^+DECs) and dendritic Thy-1^+ epidermalcells (Thy-1^+DECa) from various parts of the body wereexamined by using epidermal sheets of C3H/He inbr...Identification and enumeration of both dendritic Ia^+ epi-dermal cells (Ia^+DECs) and dendritic Thy-1^+ epidermalcells (Thy-1^+DECa) from various parts of the body wereexamined by using epidermal sheets of C3H/He inbred miceof different age groups and indirect immunofluorescent tech-nique. A significant decline of both Ia^+DECs and Thy-1^+DECs in the mice of the aged group was demonstrated anddifferent densities and different distribution patterns betweenIa^+DECs and Thy-1^+DECs were obserged. These findingsmay imply that the decline of both Ia^+DECs and Thy-1^+DECs in the aged group may reflect the alterations of im-mune response in aging.展开更多
Immune reactions to foreign or self-antigens lead to protective immunity and, sometimes, immune disorders such as allergies and autoimmune diseases. Antigen presenting cells (APC) including epidermal Langerhans cells ...Immune reactions to foreign or self-antigens lead to protective immunity and, sometimes, immune disorders such as allergies and autoimmune diseases. Antigen presenting cells (APC) including epidermal Langerhans cells (LCs) play an important role in the course and outcome of the immune reactions. Epidermal powder immunization (EPI) is a technology that offers a tool to manipulate the LCs and the potential to harness the immune reactions towards prevention and treatment of infectious diseases and immune disorders.展开更多
The epidermis is the outermost layer of skin and the first barrier against invasion.Dendritic epidermal T cells(DETCs)are a subset ofT cells and an important component of the epidermal immune microenvironment.DETCs a...The epidermis is the outermost layer of skin and the first barrier against invasion.Dendritic epidermal T cells(DETCs)are a subset ofT cells and an important component of the epidermal immune microenvironment.DETCs are involved in skin wound healing,malignancy and autoim-mune diseases.DETCs secrete insulin-like growth factor-1 and keratinocyte growth factor for skin homeostasis and re-epithelization and release inflammatory factors to adjust the inflammatory microenvironment of wound healing.Therefore,an understanding of their development,activation and correlative signalling pathways is indispensable for the regulation of DETCs to accelerate wound healing.Our review focuses on the above-mentioned molecular mechanisms to provide a general research framework to regulate and control the function of DETCs.展开更多
目的 观察脂肪来源间充质干细胞外泌体(exosomes from adipose derived mensenchymal stem cells, ADSCs-exo)对小鼠树突状表皮T细胞(dendritic epidermal T cells,DETC)及皮肤创面炎症反应调控的影响。方法 自2023年1—12月,解放军总...目的 观察脂肪来源间充质干细胞外泌体(exosomes from adipose derived mensenchymal stem cells, ADSCs-exo)对小鼠树突状表皮T细胞(dendritic epidermal T cells,DETC)及皮肤创面炎症反应调控的影响。方法 自2023年1—12月,解放军总医院第四医学中心烧伤整形医学部采用Ⅰ型胶原酶消化法提取ADSCs,收集ADSCs培养上清液利用差速超速离心法提取ADSCs-exo。原代提取并培养DETC,利用流式技术对细胞纯度进行鉴定;利用刀豆蛋白A(concanavalin A, ConA)构建体外DETC细胞炎症模型,用不同浓度的ADSCs-exo对DETC处理后,采用RT-q PCR法检测细胞中IL-2、IL-17A的m RNA表达。构建C57小鼠背部皮肤全层皮肤缺损创面模型,依次分为空白对照组、PBS注射组、ADSCs-exo注射组,蛋白质免疫印迹法检测各时间段创缘皮肤IL-17A、Akt、P-Akt表达情况;利用流式细胞分析技术观察创缘DETC表达情况。结果 DETC体外炎症模型中,ConA组IL-2、IL-17A m RNA表达量均明显高于Control组,ConA+ADSCs-exo各组IL-2、IL-17A m RNA表达量相较于ConA组不同程度降低。在小鼠创面愈合过程中可以观察到ADSCs-exo组小鼠创缘皮肤IL-17A含量要低于PBS组和对照组,而P-Akt表达量要高于PBS组和对照组。小鼠急性创伤造模后第1天,PBS组和ADSCs-exo组创缘DETC表达程度均明显高于对照组。急性创伤后第3天,PBS组创缘DETC表达量仍明显高于对照组;ADSCs-exo组DETC表达出现下降,基本降至对照组水平。结论 ConA能较好诱导活化DETC分泌表达IL-17A和IL-2,而ADSCs-exo能显著抑制其IL-2、IL-17A的表达。在小鼠创面愈合过程中,ADSCs-exo能抑制创缘皮肤IL-17A的表达、提高Akt蛋白磷酸化水平。急性创伤能提高创面DETC表达;ADSCs-exo对于DETC并无明显的趋化富集作用,但却能在较短时间内抑制DETC的表达,避免创面持续、过度的炎症反应发生。展开更多
文摘Identification and enumeration of both dendritic Ia^+ epi-dermal cells (Ia^+DECs) and dendritic Thy-1^+ epidermalcells (Thy-1^+DECa) from various parts of the body wereexamined by using epidermal sheets of C3H/He inbred miceof different age groups and indirect immunofluorescent tech-nique. A significant decline of both Ia^+DECs and Thy-1^+DECs in the mice of the aged group was demonstrated anddifferent densities and different distribution patterns betweenIa^+DECs and Thy-1^+DECs were obserged. These findingsmay imply that the decline of both Ia^+DECs and Thy-1^+DECs in the aged group may reflect the alterations of im-mune response in aging.
文摘Immune reactions to foreign or self-antigens lead to protective immunity and, sometimes, immune disorders such as allergies and autoimmune diseases. Antigen presenting cells (APC) including epidermal Langerhans cells (LCs) play an important role in the course and outcome of the immune reactions. Epidermal powder immunization (EPI) is a technology that offers a tool to manipulate the LCs and the potential to harness the immune reactions towards prevention and treatment of infectious diseases and immune disorders.
基金supported by grants from the General Program of National Natural Science Foundation of China(31872742 to WH,31970830 ,81630025 to JH,31800722 to ZM)the Military Medical Science and Technology Youth Cultivation Plan(20QNPY024)+3 种基金the Traditional Chinese Medicine Bureau of Guangdong Province(2018071 to JH)the Guangzhou Municipal Science and Technology Bureau(201904010090 to JH)the Health Commission of Guangdong Province(A2019520 to JH)a grant from the Tianjin Natural Science Foundation(19JCQNJC11400 to ZM).
文摘The epidermis is the outermost layer of skin and the first barrier against invasion.Dendritic epidermal T cells(DETCs)are a subset ofT cells and an important component of the epidermal immune microenvironment.DETCs are involved in skin wound healing,malignancy and autoim-mune diseases.DETCs secrete insulin-like growth factor-1 and keratinocyte growth factor for skin homeostasis and re-epithelization and release inflammatory factors to adjust the inflammatory microenvironment of wound healing.Therefore,an understanding of their development,activation and correlative signalling pathways is indispensable for the regulation of DETCs to accelerate wound healing.Our review focuses on the above-mentioned molecular mechanisms to provide a general research framework to regulate and control the function of DETCs.
文摘目的 观察脂肪来源间充质干细胞外泌体(exosomes from adipose derived mensenchymal stem cells, ADSCs-exo)对小鼠树突状表皮T细胞(dendritic epidermal T cells,DETC)及皮肤创面炎症反应调控的影响。方法 自2023年1—12月,解放军总医院第四医学中心烧伤整形医学部采用Ⅰ型胶原酶消化法提取ADSCs,收集ADSCs培养上清液利用差速超速离心法提取ADSCs-exo。原代提取并培养DETC,利用流式技术对细胞纯度进行鉴定;利用刀豆蛋白A(concanavalin A, ConA)构建体外DETC细胞炎症模型,用不同浓度的ADSCs-exo对DETC处理后,采用RT-q PCR法检测细胞中IL-2、IL-17A的m RNA表达。构建C57小鼠背部皮肤全层皮肤缺损创面模型,依次分为空白对照组、PBS注射组、ADSCs-exo注射组,蛋白质免疫印迹法检测各时间段创缘皮肤IL-17A、Akt、P-Akt表达情况;利用流式细胞分析技术观察创缘DETC表达情况。结果 DETC体外炎症模型中,ConA组IL-2、IL-17A m RNA表达量均明显高于Control组,ConA+ADSCs-exo各组IL-2、IL-17A m RNA表达量相较于ConA组不同程度降低。在小鼠创面愈合过程中可以观察到ADSCs-exo组小鼠创缘皮肤IL-17A含量要低于PBS组和对照组,而P-Akt表达量要高于PBS组和对照组。小鼠急性创伤造模后第1天,PBS组和ADSCs-exo组创缘DETC表达程度均明显高于对照组。急性创伤后第3天,PBS组创缘DETC表达量仍明显高于对照组;ADSCs-exo组DETC表达出现下降,基本降至对照组水平。结论 ConA能较好诱导活化DETC分泌表达IL-17A和IL-2,而ADSCs-exo能显著抑制其IL-2、IL-17A的表达。在小鼠创面愈合过程中,ADSCs-exo能抑制创缘皮肤IL-17A的表达、提高Akt蛋白磷酸化水平。急性创伤能提高创面DETC表达;ADSCs-exo对于DETC并无明显的趋化富集作用,但却能在较短时间内抑制DETC的表达,避免创面持续、过度的炎症反应发生。
