Background:Vacuum sealing drainage(VSD)and epidermal growth factor(EGF)both play an important role in the treatment of wounds.This study aims to explore the effects of the combination of VSD and EGF on wound healing a...Background:Vacuum sealing drainage(VSD)and epidermal growth factor(EGF)both play an important role in the treatment of wounds.This study aims to explore the effects of the combination of VSD and EGF on wound healing and the optimal concentration and time of EGF.Methods:We tested the proliferation and migration capacity of HaCaT and L929 cells at different EGF concentrations(0,1,5,10,and 100ng/ml)and different EGF action times(2,10,and 30min).A full-thickness skin defect model was established using male,30-week-old Bama pigs.The experiment included groups as follows:routine dressing change after covering with sterile auxiliary material(Control),continuous negative pressure drainage of the wound(VSD),continuous negative pressure drainage of the wound and injection of EGF 10min followed by removal by continuous lavage(V+E 10min),and continuous negative pressure drainage of the wound and injection of EGF 30min followed by removal by continuous lavage(V+E 30min).The wound healing rate,histological repair effect and collagen deposition were compared among the four groups.Results:An EGF concentration of 10ng/ml and an action time of 10min had optimal effects on the proliferation and migration capacities of HaCaT and L929 cells.The drug dispersion effect was better than drug infusion after bolus injection effect,and the contact surface was wider.Compared with other groups,the V+E 10min group promoted wound healing to the greatest extent and obtained the best histological score.Conclusions:A recombinant human epidermal growth factor(rhEGF)concentration of 10 ng/ml can promote the proliferation and migration of epithelial cells and fibroblasts to the greatest extent in vitro.VSD combined with rhEGF kept in place for 10min and then washed,can promote wound healing better than the other treatments in vitro.展开更多
Wound healing is a complex biologic event. Numerous growth factors are involved in the regulation of tissue repair and remodelling processes. In this study, several techniques were used to observe the effects of recom...Wound healing is a complex biologic event. Numerous growth factors are involved in the regulation of tissue repair and remodelling processes. In this study, several techniques were used to observe the effects of recombinant human epidermal growth factors (rh-EGF) on porcine partial-thickness burn wound healing. Healing rates of the burn wounds were quantitated by computer image analysis of the wound Pkotographs. Biopsies were taken for examining the cell DNA cycle by flow cytometry, and for observing the keratinocyte migration and degree of reepithelialization quantitated by anti-keratin antibodies. The results indicated that daily topical application of rh-EGF in suitable concentrations accelerated epithelial healing of partial-thickness burn wounds, and there was also a dose-dependent effect of rh-EGF on healing rates of the partial-thickness burn wounds.展开更多
Objective: To explore the relationship between substance P (SP) released from peripheral nerve endings and the expression of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) during wound heali...Objective: To explore the relationship between substance P (SP) released from peripheral nerve endings and the expression of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) during wound healing. Methods: Fifty Wistar rats were randomly divided into 2 groups, injury group and capsaicin group. In the injury group, a full thickness skin wound on the back of the rat was taken. The wound edge and granulation tissues were taken on the 1st, 3rd, 6th, 9th, 12th days after injury, respectively. In the capsaicin group, capsaicin was injected subcutaneously on the back of the rats to destroy the sensory nerve to prevent the secretion of SP, then a wound and sample was made in the same way. Immunohistochemistry and in situ hybridization were employed to detect the expression of SP, EGF/EGFR, and EGF mRNA/EGFR mRNA in the granulation tissues. Results: In the injury group, immunohistochemical stain of SP and EGF/EGFR was located on the hair follicles and sebaceous glands at the 1st day. And the stain of SP was obvious at the 3rd day in the granulation tissues, then decreased gradually. EGF/EGFR was at low level at the 3rd day, then increased gradually and reached the peak at the 9th day, then declined. In the capsaicin group, the immunohistochemical stain of SP and EGF/EGFR was faint and without obvious change during the wound healing process. The tendency of the EGF mRNA/EGFR mRNA expression was similar to that of EGF/EGFR.Conclusions: During wound healing, SP may promote the healing process by affecting the expression of EGF/EGFR in the granuation tissues.展开更多
Growth factors have recently gained clinical importance for wound management.Application of recombinant growth factors has been shown to mimic cellmigration,proliferation,and differentiation in vivo,allowing for exter...Growth factors have recently gained clinical importance for wound management.Application of recombinant growth factors has been shown to mimic cellmigration,proliferation,and differentiation in vivo,allowing for external modulation of the healing process.Perioperative drug delivery systems can enhance the biological activity of these growth factors,which have a very short in vivo half-life after topical administration.Although the basic mechanisms of these growth factors are well understood,most have yet to demonstrate a significant impact in animal studies or small-sized clinical trials.In this review,we emphasized currently approved growth factor therapies,including a sustained release system for growth factors,emerging therapies,and future research possibilities combined with surgical procedures.Approaches seeking to understand wound healing at a systemic level are currently ongoing.However,further research and consideration in surgery will be needed to provide definitive confirmation of the efficacy of growth factor therapies for intractable wounds.展开更多
Background The aim of this research was to determine the efficacy of combination therapy using an alginate dressing and mouse epidermal growth factor （mEGF） on proliferation and differentiation of epidermal stem cel...Background The aim of this research was to determine the efficacy of combination therapy using an alginate dressing and mouse epidermal growth factor （mEGF） on proliferation and differentiation of epidermal stem cells （ESCs） in patients with refractory wounds.Methods Eighteen patients （12 males and 6 females,aged from 18 to 61 years （mean 36.4 years）） with various skin wounds,were treated by dressing changing for one month.The wounds were located in the foot （11）,calf （3）,thigh （2）and forearm （2）.The patients were randomly divided into 3 groups：alginate dressing and mEGF （group A; n=6）,mEGF （group B; n=6） and control （group C; n=6）.Wound closure indexes were measured at 7,14,21 and 28 days.Samples were harvested for pathologic examination,at 7 and 14 days following treatment.Cytokeratin 10 （CK10） and cytokeratin 15 （CK15） positive cells were evaluated using the super-sensitivity （SP） immunohistochemical staining technique.Results Wound healing was promoted in groups A and B.In group A,the wound closure index was increased significantly （P 〈0.05）,and in one case the maximum cure area reached 102 cm2.Pathological examination identified a thicker epidermis,active angiogenesis and enhanced granulation in group A compared with groups B and C.Using the SP immunohistochemical staining technique,we showed that ESCs in group A were bigger in size and larger in number than in groups B and C.Overall,there was a significant difference in ESCs proliferation and differentiation between group A and group B （or C）.Conclusions Combination therapy using an alginate dressing and mEGF shows increased proliferation and differentiation of ESCs in patients with refractory wounds compared with those treated with mEGF alone.展开更多
Objective:To study the therapeutic effects of epidermal growth factor (EGF) and platelet-derived wound healing factor (PDWHF) on the nonhealing cutaneous ulcers in 78 patients with diabetes mellitus. Methods: Seventy-...Objective:To study the therapeutic effects of epidermal growth factor (EGF) and platelet-derived wound healing factor (PDWHF) on the nonhealing cutaneous ulcers in 78 patients with diabetes mellitus. Methods: Seventy-eight patients were divided into group A in which the patients were treated with saline to serve as the control, group B in which the patients were treated with EGF and group C treated with PDWHF. General healing practice was administered. Wound closure index, healing rate and histological changes of the ulcerative wounds of the patients were observed in the treatment period of 8 weeks. Results:The wond closure index and healing rate were more significantly increased in groups B and C than in group A of the control with far more angiogenesis , fibroblast hyperplasia and collagen deposition in the former than in the latter. The therapeutic effects of PDWHF seemed to be superior to those of EGF.Conclusion :It is suggested that the local application of a certain growth factor or of a combination of several growth factors in an efficient management to improve the healing of diabetic cutaneous ulcers.展开更多
目的探讨自体富血小板凝胶(APG)结合封闭负压引流(VSD)对深Ⅱ度烧伤患者创面愈合进程及表皮生长因子(EGF)、碱性成纤维细胞生长因子(bFGF)水平的影响。方法选取2018年7月—2021年7月在南通大学附属南通第三医院就诊的82例深Ⅱ度烧伤患...目的探讨自体富血小板凝胶(APG)结合封闭负压引流(VSD)对深Ⅱ度烧伤患者创面愈合进程及表皮生长因子(EGF)、碱性成纤维细胞生长因子(bFGF)水平的影响。方法选取2018年7月—2021年7月在南通大学附属南通第三医院就诊的82例深Ⅱ度烧伤患者作为研究对象,按随机数字表法分为观察组(APG结合VSD治疗)和对照组(VSD治疗),各41例。比较两组患者的疗效、创面愈合时间、肉芽生长面积、创面愈合率、住院时间、并发症,比较两组患者治疗前后的EGF、bFGF、C反应蛋白(CRP)、白细胞介素6(IL-6)、白细胞计数(WBC)、疼痛视觉模拟评分(VAS)的差值及治疗后1个月、2个月的温哥华瘢痕量表(VSS)评分的差值。结果观察组总有效率高于对照组(P<0.05)。观察组创面愈合时间、住院时间少于对照组,肉芽生长面积大于对照组,创面愈合率高于对照组(P<0.05)。观察组治疗前后EGF、bFGF、CRP、IL-6、WBC的差值高于对照组(P<0.05)。观察组治疗前与治疗后14 d VAS评分及治疗后1个月与2个月VSS评分的差值高于对照组(P<0.05)。两组患者并发症总发生率比较,差异无统计学意义(P>0.05)。结论APG结合VSD治疗深Ⅱ度烧伤可加快患者创面愈合进程,提高EGF、bFGF水平,减轻炎症反应和疼痛,减少瘢痕增生,且并发症少。展开更多
An increased number of patients with skin wounds have been witnessed in the past decades.Among the various kinds of treatments for skin wounds,topical exogenous growth factors are indispensable and have been used in m...An increased number of patients with skin wounds have been witnessed in the past decades.Among the various kinds of treatments for skin wounds,topical exogenous growth factors are indispensable and have been used in many countries.However,whether they have reliable effects remains controversial,and their application for skin wound treatment needs to be further standardized and optimized in terms of socio-economic considerations.Thus,the Chinese Burn Association developed this guideline indicating efficacy,application details,adverse reactions and precautions of five clinically common topical growth factors using the Grading of Recommendations Assessment Development and Evaluation method to promote the rational application of topical exogenous growth factors in skin wounds and to benefit more patients.展开更多
基金the National Natural Science Foundation of China(81972047,81603008,81572148).
文摘Background:Vacuum sealing drainage(VSD)and epidermal growth factor(EGF)both play an important role in the treatment of wounds.This study aims to explore the effects of the combination of VSD and EGF on wound healing and the optimal concentration and time of EGF.Methods:We tested the proliferation and migration capacity of HaCaT and L929 cells at different EGF concentrations(0,1,5,10,and 100ng/ml)and different EGF action times(2,10,and 30min).A full-thickness skin defect model was established using male,30-week-old Bama pigs.The experiment included groups as follows:routine dressing change after covering with sterile auxiliary material(Control),continuous negative pressure drainage of the wound(VSD),continuous negative pressure drainage of the wound and injection of EGF 10min followed by removal by continuous lavage(V+E 10min),and continuous negative pressure drainage of the wound and injection of EGF 30min followed by removal by continuous lavage(V+E 30min).The wound healing rate,histological repair effect and collagen deposition were compared among the four groups.Results:An EGF concentration of 10ng/ml and an action time of 10min had optimal effects on the proliferation and migration capacities of HaCaT and L929 cells.The drug dispersion effect was better than drug infusion after bolus injection effect,and the contact surface was wider.Compared with other groups,the V+E 10min group promoted wound healing to the greatest extent and obtained the best histological score.Conclusions:A recombinant human epidermal growth factor(rhEGF)concentration of 10 ng/ml can promote the proliferation and migration of epithelial cells and fibroblasts to the greatest extent in vitro.VSD combined with rhEGF kept in place for 10min and then washed,can promote wound healing better than the other treatments in vitro.
文摘Wound healing is a complex biologic event. Numerous growth factors are involved in the regulation of tissue repair and remodelling processes. In this study, several techniques were used to observe the effects of recombinant human epidermal growth factors (rh-EGF) on porcine partial-thickness burn wound healing. Healing rates of the burn wounds were quantitated by computer image analysis of the wound Pkotographs. Biopsies were taken for examining the cell DNA cycle by flow cytometry, and for observing the keratinocyte migration and degree of reepithelialization quantitated by anti-keratin antibodies. The results indicated that daily topical application of rh-EGF in suitable concentrations accelerated epithelial healing of partial-thickness burn wounds, and there was also a dose-dependent effect of rh-EGF on healing rates of the partial-thickness burn wounds.
基金MajorStateBasicResearchDevelopmentProgressofChina (No .G19990 5 4 2 0 0 )
文摘Objective: To explore the relationship between substance P (SP) released from peripheral nerve endings and the expression of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) during wound healing. Methods: Fifty Wistar rats were randomly divided into 2 groups, injury group and capsaicin group. In the injury group, a full thickness skin wound on the back of the rat was taken. The wound edge and granulation tissues were taken on the 1st, 3rd, 6th, 9th, 12th days after injury, respectively. In the capsaicin group, capsaicin was injected subcutaneously on the back of the rats to destroy the sensory nerve to prevent the secretion of SP, then a wound and sample was made in the same way. Immunohistochemistry and in situ hybridization were employed to detect the expression of SP, EGF/EGFR, and EGF mRNA/EGFR mRNA in the granulation tissues. Results: In the injury group, immunohistochemical stain of SP and EGF/EGFR was located on the hair follicles and sebaceous glands at the 1st day. And the stain of SP was obvious at the 3rd day in the granulation tissues, then decreased gradually. EGF/EGFR was at low level at the 3rd day, then increased gradually and reached the peak at the 9th day, then declined. In the capsaicin group, the immunohistochemical stain of SP and EGF/EGFR was faint and without obvious change during the wound healing process. The tendency of the EGF mRNA/EGFR mRNA expression was similar to that of EGF/EGFR.Conclusions: During wound healing, SP may promote the healing process by affecting the expression of EGF/EGFR in the granuation tissues.
文摘Growth factors have recently gained clinical importance for wound management.Application of recombinant growth factors has been shown to mimic cellmigration,proliferation,and differentiation in vivo,allowing for external modulation of the healing process.Perioperative drug delivery systems can enhance the biological activity of these growth factors,which have a very short in vivo half-life after topical administration.Although the basic mechanisms of these growth factors are well understood,most have yet to demonstrate a significant impact in animal studies or small-sized clinical trials.In this review,we emphasized currently approved growth factor therapies,including a sustained release system for growth factors,emerging therapies,and future research possibilities combined with surgical procedures.Approaches seeking to understand wound healing at a systemic level are currently ongoing.However,further research and consideration in surgery will be needed to provide definitive confirmation of the efficacy of growth factor therapies for intractable wounds.
文摘Background The aim of this research was to determine the efficacy of combination therapy using an alginate dressing and mouse epidermal growth factor （mEGF） on proliferation and differentiation of epidermal stem cells （ESCs） in patients with refractory wounds.Methods Eighteen patients （12 males and 6 females,aged from 18 to 61 years （mean 36.4 years）） with various skin wounds,were treated by dressing changing for one month.The wounds were located in the foot （11）,calf （3）,thigh （2）and forearm （2）.The patients were randomly divided into 3 groups：alginate dressing and mEGF （group A; n=6）,mEGF （group B; n=6） and control （group C; n=6）.Wound closure indexes were measured at 7,14,21 and 28 days.Samples were harvested for pathologic examination,at 7 and 14 days following treatment.Cytokeratin 10 （CK10） and cytokeratin 15 （CK15） positive cells were evaluated using the super-sensitivity （SP） immunohistochemical staining technique.Results Wound healing was promoted in groups A and B.In group A,the wound closure index was increased significantly （P 〈0.05）,and in one case the maximum cure area reached 102 cm2.Pathological examination identified a thicker epidermis,active angiogenesis and enhanced granulation in group A compared with groups B and C.Using the SP immunohistochemical staining technique,we showed that ESCs in group A were bigger in size and larger in number than in groups B and C.Overall,there was a significant difference in ESCs proliferation and differentiation between group A and group B （or C）.Conclusions Combination therapy using an alginate dressing and mEGF shows increased proliferation and differentiation of ESCs in patients with refractory wounds compared with those treated with mEGF alone.
文摘Objective:To study the therapeutic effects of epidermal growth factor (EGF) and platelet-derived wound healing factor (PDWHF) on the nonhealing cutaneous ulcers in 78 patients with diabetes mellitus. Methods: Seventy-eight patients were divided into group A in which the patients were treated with saline to serve as the control, group B in which the patients were treated with EGF and group C treated with PDWHF. General healing practice was administered. Wound closure index, healing rate and histological changes of the ulcerative wounds of the patients were observed in the treatment period of 8 weeks. Results:The wond closure index and healing rate were more significantly increased in groups B and C than in group A of the control with far more angiogenesis , fibroblast hyperplasia and collagen deposition in the former than in the latter. The therapeutic effects of PDWHF seemed to be superior to those of EGF.Conclusion :It is suggested that the local application of a certain growth factor or of a combination of several growth factors in an efficient management to improve the healing of diabetic cutaneous ulcers.
文摘目的探讨自体富血小板凝胶(APG)结合封闭负压引流(VSD)对深Ⅱ度烧伤患者创面愈合进程及表皮生长因子(EGF)、碱性成纤维细胞生长因子(bFGF)水平的影响。方法选取2018年7月—2021年7月在南通大学附属南通第三医院就诊的82例深Ⅱ度烧伤患者作为研究对象,按随机数字表法分为观察组(APG结合VSD治疗)和对照组(VSD治疗),各41例。比较两组患者的疗效、创面愈合时间、肉芽生长面积、创面愈合率、住院时间、并发症,比较两组患者治疗前后的EGF、bFGF、C反应蛋白(CRP)、白细胞介素6(IL-6)、白细胞计数(WBC)、疼痛视觉模拟评分(VAS)的差值及治疗后1个月、2个月的温哥华瘢痕量表(VSS)评分的差值。结果观察组总有效率高于对照组(P<0.05)。观察组创面愈合时间、住院时间少于对照组,肉芽生长面积大于对照组,创面愈合率高于对照组(P<0.05)。观察组治疗前后EGF、bFGF、CRP、IL-6、WBC的差值高于对照组(P<0.05)。观察组治疗前与治疗后14 d VAS评分及治疗后1个月与2个月VSS评分的差值高于对照组(P<0.05)。两组患者并发症总发生率比较,差异无统计学意义(P>0.05)。结论APG结合VSD治疗深Ⅱ度烧伤可加快患者创面愈合进程,提高EGF、bFGF水平,减轻炎症反应和疼痛,减少瘢痕增生,且并发症少。
文摘An increased number of patients with skin wounds have been witnessed in the past decades.Among the various kinds of treatments for skin wounds,topical exogenous growth factors are indispensable and have been used in many countries.However,whether they have reliable effects remains controversial,and their application for skin wound treatment needs to be further standardized and optimized in terms of socio-economic considerations.Thus,the Chinese Burn Association developed this guideline indicating efficacy,application details,adverse reactions and precautions of five clinically common topical growth factors using the Grading of Recommendations Assessment Development and Evaluation method to promote the rational application of topical exogenous growth factors in skin wounds and to benefit more patients.
