Ischemic stroke is a leading cause of death and disability worldwide.Inflammatory response after stroke determines the outcome of ischemic injury.A recent study has reported an efficient method,epidural arterial impla...Ischemic stroke is a leading cause of death and disability worldwide.Inflammatory response after stroke determines the outcome of ischemic injury.A recent study has reported an efficient method,epidural arterial implantation(EAI),for accelerating interstitial fluid(ISF)drainage,which provides a promising strategy to clear pro-inflammatory cytokines in the brain extracellular space(ECS).In this study,the method of EAI was modified(m-EAI)to control its function of accelerating the ISF drainage at different time points following ischemic attack.The neuroprotective effect of m-EAI on ischemic stroke was evaluated with the transient middle cerebral artery occlusion(tMCAO)rat model.The results demonstrated the accumulation of IL-1β,IL-6,and TNF-αwas significantly decreased by activating m-EAI at 7 d before and immediately after ischemic attack in tMCAO rats,accompanied with decreased infarct volume and improved neurological function.This study consolidates the hypothesis of exacerbated ischemic damage by inflammatory response and provides a new perspective to treat encephalopathy via brain ECS.Further research is essential to investigate whether m-EAI combined with neuroprotective drugs could enhance the therapeutic effect on ischemic stroke.展开更多
基金supported by the Major Program of the National Natural Science Foundation of China(62394310,62394313,62394314)the National Natural Science Foundation of China(12126601)+5 种基金the R&D project of Pazhou Lab(Huangpu)(2023K0608)the China Postdoctoral Science Foundation(2022M720294)Intelligent Basic Theory Innovation Research fund(201CXCY-A01-08-00-29)GuangDong Basic and Applied Basic Research Foundation(2022A1515110674)Medical Scientific Research Foundation of Guangdong Province of China(B2022067)Science Foundation of AMHT(2021YK11)。
文摘Ischemic stroke is a leading cause of death and disability worldwide.Inflammatory response after stroke determines the outcome of ischemic injury.A recent study has reported an efficient method,epidural arterial implantation(EAI),for accelerating interstitial fluid(ISF)drainage,which provides a promising strategy to clear pro-inflammatory cytokines in the brain extracellular space(ECS).In this study,the method of EAI was modified(m-EAI)to control its function of accelerating the ISF drainage at different time points following ischemic attack.The neuroprotective effect of m-EAI on ischemic stroke was evaluated with the transient middle cerebral artery occlusion(tMCAO)rat model.The results demonstrated the accumulation of IL-1β,IL-6,and TNF-αwas significantly decreased by activating m-EAI at 7 d before and immediately after ischemic attack in tMCAO rats,accompanied with decreased infarct volume and improved neurological function.This study consolidates the hypothesis of exacerbated ischemic damage by inflammatory response and provides a new perspective to treat encephalopathy via brain ECS.Further research is essential to investigate whether m-EAI combined with neuroprotective drugs could enhance the therapeutic effect on ischemic stroke.
