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Expressions of Beta-catenin,E-cadherin and MMP-7 in Ovarian Epithelial Tumors 被引量:1
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作者 林晓 李昱 米粲 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2008年第3期190-193,共4页
Objective: To investigate the expressions of Beta-catenin, E-cadherin and MMP-7 and their implications in ovarian epithelial tumor. Methods: Immunohistochemical staining with SP method was conducted to identify the ... Objective: To investigate the expressions of Beta-catenin, E-cadherin and MMP-7 and their implications in ovarian epithelial tumor. Methods: Immunohistochemical staining with SP method was conducted to identify the expressions of Beta-catenin, E-cadherin and MMP-7 in ovarian epithelial tumor in 66 cases. Results: The abnormal expression rate of Beta-catenin in malignant ovarian epithelial tumor was higher than those in borderline and benign epithelial tumors (P〈0.05). The positive rates of E-cadherin in benign and borderline ovarian epithelial tumors were significantly greater than that in malignant epithelial tumor. The expression rates of MMP-7 in malignant and borderline ovarian epithelial tumors were higher than that in benign epithelial tumor (P〈0.05). Conclusion: The abnormal expressions of Beta-catenin, E-cadherin and MMP-7 might be used to indicate the malignance transform of ovarian epithelial tumors, but they have no significant correlation with peritoneal dropsy invasion, caul invasion and appendant invasion in ovarian epithelial tumor. 展开更多
关键词 BETA-CATENIN E-cadherin MMP-7 Ovarian epithelial tumor
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Effects of Cigarette Smoke Extract on E-cadherin Expression in Cultured Airway Epithelial Cells 被引量:1
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作者 王曦 吴人亮 +1 位作者 郝天玲 陈芳 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2000年第1期32-35,共4页
Summary: To investigate whether the change of E-cadherin (ECD) expression plays a role in the injury and repair of airway epithelial cells (AEC) caused by smoking, porcine AECs were cultured by using an enzyme-dispers... Summary: To investigate whether the change of E-cadherin (ECD) expression plays a role in the injury and repair of airway epithelial cells (AEC) caused by smoking, porcine AECs were cultured by using an enzyme-dispersed method. After exposure of the AECs to cigarette smoke extract (CSE), the ECD expression in the cells was detected by using immunocytochemistry and in situ hybridization. The results showed that ECD was distributed on the plasma membrane at the cell junctions of AECs. After exposure to 20 % CSE, the membranous ECD expression was decreased, the cytoplasmic ECD expression was increased (P<0.01) as the exposure time went on. But the content of ECD mRNA in the AECs did not chang. It suggests that the change of ECD ex- pression is regulated at the posttranslational level and plays a role in the injury and repair of AEC caused by smoking. 展开更多
关键词 airway epithelial cell E-cadherin smoke inhalation injury
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肺腺癌患者CT征象表现与P53、E-cadherin、TGF-β1蛋白表达的相关性
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作者 孟锦 孙兴智 范雪微 《河南医学研究》 CAS 2024年第5期925-929,共5页
目的探讨肺腺癌患者计算机断层扫描(CT)征象表现及与抑癌基因(P53)、上皮钙黏蛋白(E-cadherin)、转化生长因子-β1(TGF-β1)蛋白表达的相关性。方法选择2020年1月至2022年12月在新乡市中心医院行手术切除的97例肺腺癌患者为研究对象,采... 目的探讨肺腺癌患者计算机断层扫描(CT)征象表现及与抑癌基因(P53)、上皮钙黏蛋白(E-cadherin)、转化生长因子-β1(TGF-β1)蛋白表达的相关性。方法选择2020年1月至2022年12月在新乡市中心医院行手术切除的97例肺腺癌患者为研究对象,采用免疫组化法检测肺腺癌组织及癌旁组织P53、E-cadherin、TGF-β1蛋白表达情况,术前采用德国西门子64排多层螺旋CT增强扫描,分析CT征象与P53、E-cadherin、TGF-β1蛋白表达的相关性。结果肺腺癌组织P53、TGF-β1蛋白阳性表达率高于癌旁组织,E-cadherin蛋白阳性表达率低于癌旁性组织(P<0.05);有棘突征、毛刺征和分叶征CT征象的肺腺癌组织中P53蛋白阳性表达率高于无棘突征、毛刺征和分叶征CT征象者(P<0.05);有棘突征、毛刺征CT征象的肺腺癌组织中E-cadherin蛋白阳性表达率低于无棘突征、毛刺征CT征象者(P<0.05);有血管集束征、毛刺征、分叶征CT征象的肺腺癌组织中TGF-β1蛋白阳性表达率高于无血管集束征、毛刺征、分叶征CT征象者(P<0.05)。结论肺腺癌组织P53、TGF-β1蛋白表达增高,E-cadherin蛋白表达降低,其阳性表达率与肺腺癌患者CT征象存在一定的相关性。 展开更多
关键词 肺腺癌 计算机断层扫描征象 抑癌基因 上皮钙黏蛋白 转化生长因子-β1 相关性
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KAI1,Snail,Slug,E-cadherin在鼻咽癌组织中的表达及其与患者预后的关系 被引量:2
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作者 马艳丽 杨靖 吕梦丽 《实用癌症杂志》 2023年第4期562-564,568,共4页
目的 探讨KAI1、Snail、Slug、上皮细胞钙黏蛋白(E-cadherin)在鼻咽癌组织中的表达及其与患者预后的关系。方法 选取43例鼻咽癌患者,收集癌组织与癌旁正常组织,采用免疫组织化学法测定其KAI1、Snail、Slug、E-cadherin表达。分析四者与... 目的 探讨KAI1、Snail、Slug、上皮细胞钙黏蛋白(E-cadherin)在鼻咽癌组织中的表达及其与患者预后的关系。方法 选取43例鼻咽癌患者,收集癌组织与癌旁正常组织,采用免疫组织化学法测定其KAI1、Snail、Slug、E-cadherin表达。分析四者与患者临床病理特征的关系;另随访3年,采用Kaplan-Meier法分析四者表达与鼻咽癌患者预后的关系。结果 癌组织中KAI1、E-cadherin阳性表达率低于癌旁组织,Snail、Slug阳性表达率高于癌旁组织,有统计学差异(P<0.05);KAI1、Snail、Slug、E-cadherin表达与鼻咽癌患者年龄、性别无关(P>0.05);KAI1、Snail、Slug、E-cadherin表达与肿瘤临床分期、分化程度、淋巴结转移有关(P<0.05);癌组织KAI1、E-cadherin阴性表达与Snail、Slug阳性表达患者的3年生存率低于KAI1、E-cadherin阳性表达与Snail、Slug阴性表达患者,有统计学差异(P<0.05)。结论 KAI1、E-cadherin在鼻咽癌组织内呈低表达,Snail、Slug呈高表达,四项指标均参与疾病的发生、发展,与患者预后相关。 展开更多
关键词 鼻咽癌 KAI1 SNAIL SLUG 上皮细胞钙黏蛋白 预后
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MiR-200c suppresses the migration of retinoblastoma cells by reversing epithelial mesenchymal transition 被引量:3
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作者 Xiao-Lei Shao Yao Chen Ling Gao 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2017年第8期1195-1202,共8页
AIM: To analyze the relationship between clinical features and epithelial mesenchymal transition (EMT) in retinoblastoma (RB), further to investigate whether miR-200c regulates the EMT and migration of RB cells. ... AIM: To analyze the relationship between clinical features and epithelial mesenchymal transition (EMT) in retinoblastoma (RB), further to investigate whether miR-200c regulates the EMT and migration of RB cells. METHODS: Expression of EMT-related markers and tumor- related factors were detected by immuno-histochemistry analysis in RB tissue from 29 cases. Correlations between their expression and clinical characteristics were analyzed. The regulation effects of miR-200c on EMT-related markers, tumor-related factors were observed in mRNA level and protein level by real-time polymerase chain reaction (PCR) and Western blot, respectively, in Y79 and Weri-rbl cells. Its effects on migration force of these RB cell lines were also detected with Transwell test. RESULTS: Lower expression of E-cadherin was present in the cases with malignant prognosis. MiR-200c promoted the expression of E-cadherin and decreased the expression of Vimentin and N-cadherin in Y79 and Weri-rbl cells. Migration force of RB cells could be inhibited by miR-200c. CONCLUSION: EMT might be associated with bad prognosis in RB. MiR-200c suppresses the migration of retinoblastomatous cells by reverse EMT. 展开更多
关键词 RETINOBLASTOMA epithelial mesenchymal transition MIR-200C E-cadherin
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Effect of Semen vaccariae and Taraxacu mogono on Cell Adhesion of Bovine Mammary Epithelial Cells 被引量:3
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作者 TONG Jin-jin LI Ye +2 位作者 LIU Rong GAO Xue-jun LI Qing-zhang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2012年第12期2043-2050,共8页
The aim of this study is to reveal the regulation mechanism of the effect of Semen vaccariae and Taraxacu mogono on the cell-cell adhersion molecule, E-cadherin and β-catenin on the proliferation role and secretion f... The aim of this study is to reveal the regulation mechanism of the effect of Semen vaccariae and Taraxacu mogono on the cell-cell adhersion molecule, E-cadherin and β-catenin on the proliferation role and secretion function of bovine mammary epithelial cells cultured in vitro. Firstly, the epithelial character of bovine mammary epithelial cells was authenticated using immunofluorescence, then the cell grow curve was observed and investigated after S. vaccariae and T. mogono treatment. On the effect of S. vaccariae and T. mogono, cell adhesion molecules E-cadherin, β-catenin and CycinD1 mRNA and protein were detected by qRT-PCR and Western blotting, respectively. The results showed that the cellular keratin 18 expressed positively and proliferfated vigorously after S. vaccariae and T. mogono treament. The mRNA and protein levels of E-cadherin and CycinD1 were remarkably higher (P〈0.05) in 36 h after S. vaccariae and T. mogono treatment. The cell proliferation at 36 h was increased significantly (P〈0.05). In conclusion, S. vaccariae and T. mogono have a positive impact on the cell proliferation and an effect on the adhesion molecules E-cadherin, β-catenin and CycinD1 in the Wnt signaling pathway. 展开更多
关键词 E-cadherin Β-CATENIN Semen vaccariae Taraxacu mogono BOVINE mammary epithelial cells
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Interference of suppressor of cytokine signaling 3 promotes epithelial-mesenchymal transition in MHCC97H cells 被引量:1
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作者 Yuan-Yuan Ji Zhi-Dong Wang +1 位作者 Zong-Fang Li Ke Li 《World Journal of Gastroenterology》 SCIE CAS 2013年第6期866-873,共8页
AIM:To investigate the role of suppressor of cytokine signaling 3 (SOCS3) silencing in epithelial-mesenchymal transition (EMT) involved in a human hepatocellular carcinoma MHCC97H cell line.METHODS:MHCC97H cells were ... AIM:To investigate the role of suppressor of cytokine signaling 3 (SOCS3) silencing in epithelial-mesenchymal transition (EMT) involved in a human hepatocellular carcinoma MHCC97H cell line.METHODS:MHCC97H cells were transiently transfected with SOCS3 small-interfering RNA (siRNA).Morphological changes of the transfected cells were observed under microscope.Expressions of E-cadherin,Vimentinand α-smooth muscle actin (α-SMA) were identified with immunofluorescence.Furthermore,protein expressions and mRNA levels of characteristic markers of EMT (E-cadherin,Vimentin,α-SMA and Snail) were detected by Western blotting,quantitative real-time polymerase chain reaction.Transforming growth factor-β1 (TGF-β1) levels in the supernatant were measured with enzyme-linked immunosorbent assay.RESULTS:The transfected cells with SOCS3 siRNA showed a morphological alteration from a typical cobblestone morphology to mesenchymal spindle-shaped and fusiform features.SOCS3 siRNA lessened immunofluorescent expression of E-cadherin,but elicited immunofluorescent expressions of Vimentin and α-SMA in MHCC97H cells.More importantly,compared with the negative control,depletion of SOCS3 resulted in the decrease of the epithelial marker E-cadherin (P < 0.05),and the increase of the mesenchymal markers Vimentin and α-SMA and the transcription factor Snail in MHCC97H cells (P < 0.05).Moreover,compared with the negative control,SOCS3 siRNA evidently enhanced TGF-β1 secretion in MHCC97H cells (200.20 ± 29.02 pg/mL vs 490.20 ± 92.43 pg/mL,P < 0.05).CONCLUSION:SOCS3 silencing is able to promote EMT in MHCC97H cells via changing the phenotypic characteristics and modulating the characteristic markers. 展开更多
关键词 Hepatocellular carcinoma epithelial-mesenchymal transition SUPPRESSOR of CYTOKINE signaling E-cadherin SNAIL
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Expression of transcription factors Slug in the lens epithelial cells undergoing epithelial-mesenchymal transition induced by connective tissue growth factor 被引量:1
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作者 Ying-Na Wang Li Qin +2 位作者 Jing-Ming Li Li Chen Cheng Pei 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2015年第5期872-876,共5页
AIMTo investigate the expression of transcription factors Slug in human lens epithelial cells (HLECs) undergoing epithelial-mesenchymal transition (EMT) induced by connective tissue growth factor (CTGF).METHODSHLECs w... AIMTo investigate the expression of transcription factors Slug in human lens epithelial cells (HLECs) undergoing epithelial-mesenchymal transition (EMT) induced by connective tissue growth factor (CTGF).METHODSHLECs were treated with CTGF of different concentrations (20, 50 and 100 ng/mL) or without CTGF (control) for 24h. The morphological changes of HLECs were analysed by microscopy. The expression and cellular localization of Slug was evaluated by immumo-fluorescence. Expressions of Slug, E-cadherin and alpha smooth muscle actin (&#x003b1;-SMA) were further determined by Western blot analysis.RESULTSHLECs showed spidle fibrolasts-like characteristics and loosely connected each other after CTGF treatment. The immuno-fluorescence staining indicated that Slug was localized in the nuclei and its expression was induced by CTGF. The relative expressions of Slug protein were 1.64&#x000b1;0.11, 1.96 &#x000b1;0.03, 3.12 &#x000b1;0.10, and 4.08&#x000b1;0.14, respectively, in response to control group and treatment with CTGF of 20, 50 and 100 ng/mL (F=443.86, P&#x0003c;0.01). The increased Slug protein levels were correlated well with up-expression of &#x003b1;-SMA (0.78&#x000b1;0.05, 0.85&#x000b1;0.06, 2.17&#x000b1;0.15, 2.86&#x000b1;0.10; F=449.85, P&#x0003c;0.01) and down-expression of E-cadherin (2.50&#x000b1;0.11, 1.79&#x000b1;0.26, 1.05&#x000b1;0.14, 0.63&#x000b1;0.08; F=101.55, P&#x0003c;0.01).CONCLUSIONTranscription factor Slug may be involved in EMT of HLECs induced by CTGF in vitro. 展开更多
关键词 transcription factors Slug human lens epithelial cells connective tissue growth factor epithelial-mesenchymal transition alpha smooth muscle actin adhesion molecules E-cadherin
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益肾通癃汤对前列腺癌模型裸鼠N-cadherin、Vimentin蛋白表达的影响 被引量:2
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作者 向时竹 刘德果 +3 位作者 赵姣 苏艺峰 林梦姣 陈其华 《中医药导报》 2023年第3期17-22,共6页
目的:探究益肾通癃汤对前列腺癌(PCa)皮下种植瘤模型裸鼠N-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)表达的影响。方法:用前列腺癌PC-3细胞悬液建立PCa皮下种植瘤模型裸鼠,造模成功后将其随机分为模型组、益肾通癃汤低剂量组、益肾通... 目的:探究益肾通癃汤对前列腺癌(PCa)皮下种植瘤模型裸鼠N-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)表达的影响。方法:用前列腺癌PC-3细胞悬液建立PCa皮下种植瘤模型裸鼠,造模成功后将其随机分为模型组、益肾通癃汤低剂量组、益肾通癃汤中剂量组、益肾通癃汤高剂量组,每组10只。各给药组灌胃给予相应药物,模型组灌胃给予等体积生理盐水,2次/d,连续3周。给药结束后,观察裸鼠一般状态,测量体质量及瘤体大小,称重并计算抑瘤率;运用HE染色观察各组裸鼠种植瘤病理学改变;ELISA法检测各组裸鼠血清中N-cadherin、Vimentin的水平;Western blotting检测各组裸鼠种植瘤组织中N-cadherin、Vimentin蛋白表达情况;PCR检测各组裸鼠种植瘤组织中N-cadherin m RNA、Vimentin m RNA表达情况。结果:与模型组比较,益肾通癃汤低、中、高剂量组裸鼠状态均可,瘤体质量,血清中N-cadherin、Vimentin水平,种植瘤组织中N-cadherin、Vimentin的蛋白及m RNA相对表达量均降低(P<0.05),瘤体细胞少量坏死;与益肾通癃汤低剂量组比较,益肾通癃汤中、高剂量组裸鼠一般状态可,瘤体质量,血清中N-cadherin、Vimentin水平、种植瘤组织中N-cadherin、Vimentin蛋白及mRNA相对表达量均降低(P<0.05),瘤体细胞部分坏死;与益肾通癃汤中剂量组比较,益肾通癃汤高剂量组裸鼠一般状态良好,瘤体质量,血清中N-cadherin水平,种植瘤组织中N-cadherin、Vimentin蛋白相对表达量,种植瘤组织中Vimentin mRNA相对表达量均降低(P<0.05),瘤体细胞坏死多;益肾通癃汤高剂量组裸鼠血清中Vimentin水平、种植瘤组织中N-cadherin mRNA相对表达量与益肾通癃汤中剂量组比较,差异无统计学意义(P>0.05);益肾通癃汤低、中、高剂量组裸鼠抑瘤率分别为26.67%、42.45%、66.26%。结论:益肾通癃汤对人前列腺癌PC-3细胞皮下种植瘤模型裸鼠N-cadherin、Vimentin蛋白的表达具有一定的抑制作用,其机制可能与抑制肿瘤上皮间质转化(EMT)过程相关。 展开更多
关键词 前列腺癌 益肾通癃汤 种植瘤 上皮间质转化 N-cadherin VIMENTIN 小鼠
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miR-221与E-cadherin在肾透明细胞癌中的表达及临床意义
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作者 詹江辉 杨雪 +1 位作者 狄文玉 鲁广建 《河南医学研究》 CAS 2023年第4期583-588,共6页
目的研究miR-221及E-cadherin在肾透明细胞癌中的表达并分析两者的相关性,探讨miR-221表达对肾癌患者生存时间的影响以及预后的关系。方法选取经病理确诊的38例肾透明细胞癌患者为研究对象,手术后收集癌组织标本作为实验组,同时收集癌... 目的研究miR-221及E-cadherin在肾透明细胞癌中的表达并分析两者的相关性,探讨miR-221表达对肾癌患者生存时间的影响以及预后的关系。方法选取经病理确诊的38例肾透明细胞癌患者为研究对象,手术后收集癌组织标本作为实验组,同时收集癌旁正常组织标本作为对照组,采用实时荧光定量PCR的方法分别检测实验组及对照组中miR-221的表达,分析miR-221与肾透明细胞癌患者临床病理参数的关系。对癌组织及癌旁组织病理切片进行免疫组织化学染色并检测E-cadherin的表达情况,分析E-cadherin与肾透明细胞癌患者临床病理参数之间的关系。Spearman相关性分析E-cadherin的表达与miR-221表达的相关性。Kaplan-Meier生存分析和log-rank检验评估肾透明细胞癌患者癌组织中miR-221表达与患者生存预后的关系。结果实验组中miR-221表达量(4.911±1.064)高于对照组(1.163±0.238),差异有统计学意义(P<0.05)。癌组织中,miR-221的相对表达量与Fuhrman分级、淋巴结转移有关(P<0.05),与年龄、性别、肿瘤大小、肾静脉内癌栓无关(P>0.05)。免疫组织化学染色结果显示E-cadherin在实验组中表达阳性率为26.32%(10/38),对照组表达阳性率为81.58%(31/38),两组表达差异有统计学意义(P<0.05)。E-cadherin的表达与年龄、性别、肿瘤大小、淋巴结转移、癌栓形成无关(P>0.05),与Fuhrman分级有关(P<0.05)。Spearman相关性分析结果显示E-cadherin的表达与miR-221的表达呈负相关,相关系数r为-0.569(P<0.001)。Kaplan-Meier生存曲线研究miR-221的表达与患者生存时间的关系,结果显示miR-221高表达组5 a生存率为52.94%(9/17),miR-221低表达组5 a生存率80.95%(17/21),log-rank检验评估miR-221高表达组与miR-221低表达组生存曲线的差异,结果显示差异有统计学意义(P<0.05)。结论miR-221在肾细胞癌中表达升高且与临床Fuhrman分级及淋巴结转移有关,miR-221与上皮-间质转化(EMT)相关分子E-cadherin的表达有相关性。本研究表明miR-221在肾透明细胞癌EMT进展过程中发挥一定的作用,是肾透明细胞癌患者生存及预后评估的潜在标志物。 展开更多
关键词 肾透明细胞癌 MIR-221 E-cadherin 上皮-间质转化
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Epithelial-to-mesenchymal transition in pancreatic ductal adenocarcinoma: Characterization in a 3D-cell culture model
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作者 Nicoletta Gagliano Giuseppe Celesti +7 位作者 Lorenza Tacchini Stefano Pluchino Chiarella Sforza Marco Rasile Vincenza Valerio Luigi Laghi Vincenzo Conte Patrizia Procacci 《World Journal of Gastroenterology》 SCIE CAS 2016年第18期4466-4483,共18页
AIM: To analyze the effect of three-dimensional (3D)-arrangement on the expression of epithelial-to-mesenchymal transition markers in pancreatic adenocarcinoma (PDAC) cells.METHODS: HPAF-II, HPAC, and PL45 PDAC cells ... AIM: To analyze the effect of three-dimensional (3D)-arrangement on the expression of epithelial-to-mesenchymal transition markers in pancreatic adenocarcinoma (PDAC) cells.METHODS: HPAF-II, HPAC, and PL45 PDAC cells were cultured in either 2D-monolayers or 3D-spheroids. Ultrastructure was analyzed by transmission electron microscopy. The expression of E-cadherin, &#x003b2;-catenin, N-cadherin, collagen type&#x02005;I&#x02005;(COL-I), vimentin, &#x003b1;-smooth muscle actin (&#x003b1;SMA), and podoplanin was assayed by confocal microscopy in cells cultured on 12-mm diameter round coverslips and in 3D-spheroids. Gene expression for E-cadherin, Snail, Slug, Twist, Zeb1, and Zeb2 was quantified by real-time PCR. E-cadherin protein level and its electrophoretic pattern were studied by Western blot in cell lysates obtained from cells grown in 2D-monolayers and 3D-spheroids.RESULTS: The E-cadherin/&#x003b2;-catenin complex was expressed in a similar way in plasma membrane cell boundaries in both 2D-monolayers and 3D-spheroids. E-cadherin increased in lysates obtained from 3D-spheroids, while cleavage fragments were more evident in 2D-monolayers. N-cadherin expression was observed in very few PDAC cells grown in 2D-monolayers, but was more evident in 3D-spheroids. Some cells expressing COL-I were observed in 3D-spheroids. Podoplanin, expressed in collectively migrating cells, and &#x003b1;SMA were similarly expressed in both experimental conditions. The concomitant maintenance of the E-cadherin/&#x003b2;-catenin complex at cell boundaries supports the hypothesis of a collective migration for these cells, which is consistent with podoplanin expression.CONCLUSION: We show that a 3D-cell culture model could provide deeper insight into understanding the biology of PDAC and allow for the detection of marked differences in the phenotype of PDAC cells grown in 3D-spheroids. 展开更多
关键词 epithelial-to-mesenchymal transition E-cadherin 3D-spheroids PODOPLANIN Pancreatic ductal adenocarcinoma
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不同组织内YAP1、E-cadherin表达与上皮性卵巢癌患者临床病理特征间的关系分析
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作者 夏潇 王莹 +1 位作者 韩灵云 韩恩善 《宁夏医学杂志》 CAS 2023年第8期695-698,F0003,共5页
目的研究不同组织内Yes相关蛋白1(YAP1)、上皮钙黏蛋白(E-cadherin)表达与上皮性卵巢癌患临床病理特征间的关系。方法回顾性分析确诊为上皮性卵巢癌并行卵巢切除术的57例患者的石蜡包埋的肿瘤组织标本,同时,收集石蜡包埋的癌旁正常卵巢... 目的研究不同组织内Yes相关蛋白1(YAP1)、上皮钙黏蛋白(E-cadherin)表达与上皮性卵巢癌患临床病理特征间的关系。方法回顾性分析确诊为上皮性卵巢癌并行卵巢切除术的57例患者的石蜡包埋的肿瘤组织标本,同时,收集石蜡包埋的癌旁正常卵巢组织标本,对癌组织及癌旁组织分别利用免疫组化法检测YAP1、E-cadherin表达。比较不同组织内YAP1、E-cadherin阳性检出率,不同临床病理特征上皮性卵巢癌患者YAP1、E-cadherin阳性检出率差异,对57例患者行3年院外随访,观察癌组织YAP1及E-cadherin阳性、阴性患者术后3年存活率差异。结果癌组织YAP1阳性检出53例(92.98%),癌旁组织YAP1阳性检出率7例(12.28%),癌组织E-cadherin阳性检出27例(47.37%),癌旁组织E-cadherin阳性检出2例(3.51%),癌组织YAP1及E-cadherin阳性检出率高于癌旁组织(P<0.05)。FIGO分期为Ⅲ~Ⅳ期患者癌组织YAP1阳性检出率明显增高(P<0.05);FIGO分期Ⅰ~Ⅱ期、分化程度高、淋巴结未转移患者癌组织E-cadherin阳性检出率增高(P<0.05)。57例经卵巢切除术治疗的患者3年死亡23例(40.35%),存活34例(59.65%);Kaplan-Meier生存分析显示,癌组织E-cadherin阳性检出率越高患者存活率越高(Log-Rank=4.752,P<0.05)。结论在上皮性卵巢癌中,YAP1呈高水平表达,且与临床分期有显著相关性;E-cadherin在上皮卵巢癌中表达上调,与疾病进展、转移等有关,但其表达随着临床分期、分化程度、淋巴结转移情况增加而下降,一定程度上可用来预测患者预后。 展开更多
关键词 上皮性卵巢癌 Yes相关蛋白1 上皮钙黏蛋白 预后
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FOXC1和E-Cadherin表达水平与肝癌临床预后的关系及对肝癌细胞生物学行为的影响
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作者 聂媛媛 赵恒芳 +1 位作者 王英娟 赵艳 《肝脏》 2023年第12期1446-1449,1454,共5页
目的探讨叉头框转录基因C1(FOXC1)和上皮钙黏蛋白(E-Cadherin)在肝癌组织中的表达,并分析与预后相关性及对癌细胞生物学行为的影响。方法将2017年4月—2022年4月在西北大学附属医院/西安市第三医院接受根治术治疗的85例原发性肝细胞癌... 目的探讨叉头框转录基因C1(FOXC1)和上皮钙黏蛋白(E-Cadherin)在肝癌组织中的表达,并分析与预后相关性及对癌细胞生物学行为的影响。方法将2017年4月—2022年4月在西北大学附属医院/西安市第三医院接受根治术治疗的85例原发性肝细胞癌患者为研究对象,均术中采集癌灶组织和癌旁组织,通过免疫组织化学法测定FOXC1、E-Cadherin的表达水平,并分析两者表达与患者预后的相关性。并选用细胞系HepG2,分为sh FOXC1、sh NC及空白对照组,通过平板克隆实验、Transwell实验测定细胞的增殖与侵袭能力。结果通过测定,本组病例肝癌组织中FOXC1蛋白和E-Cadherin阳性表达率分别为74.12%、28.24%,癌旁组织分别为43.53%、100.00%,差异存在统计学意义(P<0.05)。TNM分期是Ⅲ~Ⅳ期、低中分化、肿瘤直径≥3cm患者的FOXC1阳性表达率高,E-cadherin阳性表达率低,差异均存在统计学意义(P<0.05)。通过Spearman分析显示,在肝癌组织中FOXC1表达与预后呈负相关(P<0.05),E-Cadherin表达水平与预后呈正相关(P<0.05)。通过细胞实验,空白对照组、sh NC组FOXC1表达水平高于正常组,而E-cadherin表达低于正常组,差异均存在统计学意义(P<0.05)。在敲低FOXC1后,癌细胞增殖、侵袭等能力均显著减弱(P<0.05)。结论FOXC1、E-cadherin表达和肝癌治疗预后存在相关性,前者通过促进癌细胞增殖,并抑制后者表达促进上皮-间充质转化进程,增强肝癌细胞侵袭性,影响到临床预后。 展开更多
关键词 叉头框蛋白C1 上皮钙黏素 肝癌 预后 生物学行为
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血清miR-141-3p、sE-cadherin、COX-2水平在乳腺癌中的表达及意义 被引量:1
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作者 王聪 张娜贤 +1 位作者 周阳 王海彦 《分子诊断与治疗杂志》 2023年第8期1422-1425,1430,共5页
目的探讨血清miR-141-3p、可溶性上皮钙黏蛋白(s E-cadherin)、环氧合酶-2(COX-2)水平在乳腺癌中的表达及与临床病理、细胞免疫的关系。方法选择2020年4月至2021年12月南阳市第二人民医院收治的乳腺癌患者50例进行研究,设为病例组;并选... 目的探讨血清miR-141-3p、可溶性上皮钙黏蛋白(s E-cadherin)、环氧合酶-2(COX-2)水平在乳腺癌中的表达及与临床病理、细胞免疫的关系。方法选择2020年4月至2021年12月南阳市第二人民医院收治的乳腺癌患者50例进行研究,设为病例组;并选取同期入院的乳腺良性疾病患者50例设为对照组。采用酶联免疫分析两组患者miR-141-3p、sE-cadherin、COX-2表达及与临床病理、细胞免疫之间的关系。结果病例组血清miR-141-3p、sE-cadherin、COX-2水平显著高于对照组,差异均有统计学意义(t=24.400,8.229,26.195,P<0.01);病例组CD4+、CD4+/CD8+水平显著低于对照组,CD8+显著高于对照组,差异均有统计学意义(t=19.419,170.271,203.192,P<0.01);所有患者不同年龄、病理组织学类型与血清miR-141-3p、sE-cadherin、COX-2表达水平比较差异无统计学意义(P>0.05);肿瘤最大径>4 cm、病理分级Ⅲ级~Ⅳ级、分化程度高分化程度、淋巴结转移血清miR-141-3p、sE-cadherin、COX-2水平均明显高于肿瘤最大径≤4 cm、病理分级Ⅰ~Ⅱ级级、中低分化程度、淋巴结未转移患者,差异有统计学意义(P<0.05);相关性分析显示,血清miR-141-3p、sE-cadherin、COX-2与乳腺癌患者肿瘤最大径、病理分级、分化程度、淋巴结转移及CD8+之间均呈正相关,血清miR-141-3p、sE-cadherin、COX-2与乳腺癌患者CD4+、CD4+/CD8+之间均呈负相关(P<0.05)。结论乳腺癌患者中血清miR-141-3p、sE-cadherin、COX-2水平表达明显升高,与临床病理、细胞免疫之间关系密切,可能协同参与了乳腺癌的发生、发展,对乳腺癌患者预后的评估具有重要意义。 展开更多
关键词 miR-141-3p 可溶性上皮钙黏蛋白 环氧合酶-2 乳腺癌 临床病理 细胞免疫
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Epithelial-Mesenchymal Transition and Breast Cancer Stem Cells in Breast Cancer Progression
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作者 Wanyue Han Qingshan Li +1 位作者 Qing Zhang Fan Xu 《Advances in Breast Cancer Research》 2022年第2期141-151,共11页
Breast cancer stem cells (BCSCs) are a small subpopulation of cancer cells having the ability of self-renewing and multi-lineage differentiation, which have also been termed as “tumor-initiating cells”. And in recen... Breast cancer stem cells (BCSCs) are a small subpopulation of cancer cells having the ability of self-renewing and multi-lineage differentiation, which have also been termed as “tumor-initiating cells”. And in recent years, the role of epithelial mesenchymal transition (EMT) in malignant tumors has been valued. This paper will briefly review and discuss the relationship between BCSCs and EMT. 展开更多
关键词 epithelial-Mesenchymal Transition Breast Cancer Stem Cells E-cadherin VIMENTIN Signaling Pathway
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HIF-1α、E-Cadherin、Snail+Slug在宫颈鳞癌中的表达及意义
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作者 郅玲然 任雨洁 +3 位作者 李元朋 蔡凤梅 王卉芳 郭海燕 《临床医学研究与实践》 2023年第28期1-4,17,共5页
目的研究宫颈鳞癌中缺氧诱导因子-1α(HIF-1α)与上皮间充质转化(EMT)相关蛋白E-Cadherin、Snail+Slug的表达及其相关性。方法运用基因芯片免疫组织化学方法检测HIF-1α、E-Cadherin、Snail+Slug在20例正常宫颈组织(NC组)和80例宫颈鳞... 目的研究宫颈鳞癌中缺氧诱导因子-1α(HIF-1α)与上皮间充质转化(EMT)相关蛋白E-Cadherin、Snail+Slug的表达及其相关性。方法运用基因芯片免疫组织化学方法检测HIF-1α、E-Cadherin、Snail+Slug在20例正常宫颈组织(NC组)和80例宫颈鳞癌组织(ICC组)中的表达。结果HIF-1α、E-Cadherin、Snail+Slug在ICC组的阳性表达率分别为77.50%(62/80)、67.50%(54/80)、36.25%(29/80),在NC组的阳性表达率分别为5.00%(1/20)、95.00%(19/20)、0(0/20);HIF-1α、E-Cadherin及Snail+Slug在NC组及ICC组中的阳性表达率比较,差异具有统计学意义(P<0.05)。80例宫颈鳞癌患者中,HIF-1α阳性62例(77.50%),E-Cadherin阳性54例(67.50%),Snail+Slug阳性29例(36.25%)。临床分期Ⅱ期患者的HIF-1α阳性率高于临床分期Ⅰ期患者(P<0.05);临床分期Ⅰ期、高+中分化程度、浸润深度<1/2宫颈间质、无淋巴结转移患者的E-Cadherin阳性率分别高于临床分期Ⅱ期、低分化程度、浸润深度≥1/2宫颈间质、有淋巴结转移患者(P<0.05);低分化程度、浸润深度≥1/2宫颈间质患者的HIF-1α、Snail+Slug阳性率分别高于高+中分化程度、浸润深度<1/2宫颈间质患者(P<0.05);有淋巴结转移患者的Snail+Slug阳性率高于无淋巴结转移患者(P<0.05)。相关性分析结果显示,HIF-1α与E-Cadherin的表达呈负相关,与Snail+Slug的表达呈正相关(P<0.05)。结论HIF-1α蛋白的表达水平与宫颈鳞癌的分化程度、浸润深度密切相关。HIF-1α的过表达可能通过EMT途径促进宫颈鳞癌的浸润与转移,联合检测HIF-1α蛋白表达可作为评价患者预后的生物学指标。 展开更多
关键词 宫颈鳞癌 缺氧诱导因子-1Α E-cadherin Snail+Slug 上皮间充质转化
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高糖诱导下人脐静脉内皮细胞形态、增殖活性及钙黏蛋白表达变化
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作者 王霞 韩居才 +1 位作者 刘凯歌 何腾飞 《山东医药》 CAS 2024年第16期38-41,共4页
目的探讨不同糖浓度状态下人脐静脉内皮细胞(HUVECs)形态、增殖活性及VE-钙黏蛋白(VE-cadherin)、E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)表达变化。方法将HUVECs分为正常对照组(葡萄糖浓度5.5 mmol/L)、甘露醇组(葡萄糖浓度5.... 目的探讨不同糖浓度状态下人脐静脉内皮细胞(HUVECs)形态、增殖活性及VE-钙黏蛋白(VE-cadherin)、E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)表达变化。方法将HUVECs分为正常对照组(葡萄糖浓度5.5 mmol/L)、甘露醇组(葡萄糖浓度5.5 mmol/L+甘露醇浓度24.5 mmol/L)、高糖组(葡萄糖浓度分别为11.1、22.2、33.3 mmol/L),分别培养24、48、72 h,以显微镜观察细胞形态,用CCK-8法检测各组细胞增殖情况,Western blotting法检测各组HUVECs VE-cadherin、E-cadherin、N-cadherin表达。结果培养72 h,正常对照组细胞形态呈类圆形,高糖组、甘露醇组细胞形态由圆形、椭圆形逐渐到条索状,细胞数量减少、细胞间隙较前增加。培养24h,高糖组细胞增殖率高于正常对照组;随糖浓度升高,高糖组细胞增殖率呈现先升高后降低趋势。培养48、72h,高糖组细胞增殖率低于正常对照组;随着糖浓度升高,高糖组细胞增殖率逐渐降低。以上各组细胞增殖率比较均有统计学差异(P均<0.05)。培养24、72 h,与正常对照组比较,高糖组HUVECsN-cadherin、VE-cadherin、E-cadherin蛋白表达明显升高(P均<0.05);且随着葡萄糖浓度升高,高糖组细胞N-cadherin、VE-cadherin、E-caderin蛋白表达呈现先升高后降低的趋势。结论在高糖条件下,HUVECs发生形态转变,增殖率下降,且伴有细胞钙黏蛋白N-cadherin、VE-cadherin、E-cadherin表达变化。 展开更多
关键词 糖尿病微血管病变 人脐静脉内皮细胞 高糖 钙黏蛋白 上皮间充质转化 黏附分子
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黄芩素对人乳腺癌细胞系MDA-MB-231侵袭、迁移、上皮间充质转化的调控作用及其机制
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作者 陈林 梁秋果 +1 位作者 吉杨丹 王恒 《山东医药》 CAS 2024年第6期10-13,共4页
目的观察黄岑素对人乳腺癌细胞系MDA-MB-231的侵袭、迁移及上皮间充质转化(EMT)的调控作用,探讨其可能作用机制。方法取对数生长期MDA-MB-231细胞分为一组、二组、三组及对照组,一组、二组、三组分别加入2.5、5、10μmol/L的黄岑素,对... 目的观察黄岑素对人乳腺癌细胞系MDA-MB-231的侵袭、迁移及上皮间充质转化(EMT)的调控作用,探讨其可能作用机制。方法取对数生长期MDA-MB-231细胞分为一组、二组、三组及对照组,一组、二组、三组分别加入2.5、5、10μmol/L的黄岑素,对照组不做任何处理。培养48 h时采用划痕修复实验观察四组细胞迁移能力、采用Transwell侵袭实验观察四组细胞侵袭能力,采用Western Blotting法检测细胞EMT标志物波形蛋白(vimentin)及E-钙黏蛋白(E-cadherin)、整合素αv、β3、磷酸化黏着斑激酶(p-FAK)、磷酸化磷脂酰肌醇3激酶(整合素p-PI3K)。结果与对照组相比,黄岑素组细胞迁移率降低、侵袭细胞数少,细胞E-cadherin相对表达量高,vimentin、整合素αv、整合素β3、p-FAK、p-PI3K蛋白相对表达量低,且呈剂量依赖性(P均<0.05)。结论黄芩素抑制MDA-MB-231细胞的侵袭、迁移及EMT。黄岑素可能通过抑制整合素αv、整合素β3表达,进一步抑制p-FAK、p-PI3K蛋白表达,抑制MDA-MB-231的侵袭、迁移及EMT。 展开更多
关键词 黄芩素 乳腺癌 细胞侵袭 细胞迁移 上皮间质转化 波形蛋白 E-钙黏蛋白 整合素αv、整合素β3 黏着斑激酶 磷脂酰肌醇3激酶
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Snail与E-cadherin在上皮性肿瘤中的表达及其临床意义 被引量:14
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作者 张阿丽 王全胜 +4 位作者 陈刚 钟亚华 谢丛华 周云峰 马丁 《肿瘤》 CAS CSCD 北大核心 2006年第5期469-471,共3页
目的:探讨Snail与E-cadherin蛋白表达在上皮性肿瘤中的临床意义。方法:应用免疫组织化学SP法,研究150例人肝癌、乳腺癌、宫颈癌、子宫内膜癌与卵巢癌临床标本及30例相应正常组织中Snail与E-cadherin蛋白的表达及定位。结果:在上述5种组... 目的:探讨Snail与E-cadherin蛋白表达在上皮性肿瘤中的临床意义。方法:应用免疫组织化学SP法,研究150例人肝癌、乳腺癌、宫颈癌、子宫内膜癌与卵巢癌临床标本及30例相应正常组织中Snail与E-cadherin蛋白的表达及定位。结果:在上述5种组织中,Snail主要表达于癌组织细胞核、细胞质,相应正常组织往往缺如,且在肝癌、乳腺癌中Snail表达与肿瘤转移有一定的相关性,并与E-cadherin表达呈负相关。E-cadherin定位于细胞膜,在肝、乳腺的正常组织中E-cadherin强表,相应癌组织中其表达往往下降或缺如;而在宫颈癌、内膜癌与卵巢癌中,E-cadherin表达与癌变无明显相关性。结论:Snail、E-cadherin的表达在肝癌、乳腺癌中具有重要意义,Snail有可能作为肝癌、乳腺癌肿瘤转移的生物学标志。 展开更多
关键词 肿瘤 腺性和上皮性 免疫组织化学 基因表达 SNAIL E-cadherin
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宫腔粘连子宫内膜组织中ArhGAP29、E-cadherin的表达及意义 被引量:14
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作者 徐倩 段华 +3 位作者 汪沙 甘露 付凤仙 汤一群 《临床与实验病理学杂志》 CAS CSCD 北大核心 2017年第1期4-7,共4页
目的探讨ArhGAP29和E-cadherin在宫腔粘连(intrauterine adhesions,IUAs)子宫内膜组织中的表达及其临床意义,分析ArhGAP29、E-cadherin在IUAs发病中的作用机制。方法采用免疫组化PV 9000两步法检测IUAs中ArhGAP29、E-cadherin的表达,分... 目的探讨ArhGAP29和E-cadherin在宫腔粘连(intrauterine adhesions,IUAs)子宫内膜组织中的表达及其临床意义,分析ArhGAP29、E-cadherin在IUAs发病中的作用机制。方法采用免疫组化PV 9000两步法检测IUAs中ArhGAP29、E-cadherin的表达,分析其与IUAs的临床病理特征关系以及ArhGAP29、E-cadherin的相关性。结果(1)ArhGAP29、E-cadherin在正常子宫内膜中表达的免疫组化反应评分(immunoreactive score,IRS)高于IUAs患者,差异有统计学意义(P=0.017,P=0.004);(2)中度IUAs患者子宫内膜中ArhGAP29、E-cadherin表达的IRS高于重度IUAs患者,差异有统计学意义(P=0.020,P=0.026);非闭经IUAs患者子宫内膜ArhGAP29、E-cadherin表达的IRS高于闭经IUAs患者,差异有统计学意义(P=0.019,P=0.031);(3)ArhGAP29表达降低与E-cadherin低表达之间呈显著正相关(r=0.725,P<0.001)。结论 ArhGAP29、E-cadherin在IUAs子宫内膜中表达降低,其下降程度与IUAs严重程度有关,ArhGAP29、E-cadherin降低可能参与IUAs的形成。 展开更多
关键词 宫腔粘连 ArhGAP29 E-cadherin 上皮一间质转化 免疫组织化学
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