Library construction is a common method used to screen target genes in molecular biology.Most library constructions are not suitable for a small DNA library(<100 base pair(bp))and low RNA library output.To maximize...Library construction is a common method used to screen target genes in molecular biology.Most library constructions are not suitable for a small DNA library(<100 base pair(bp))and low RNA library output.To maximize the library’s complexity,error-prone polymerase chain reaction(PCR)was used to increase the base mutation rate.After introducing the DNA fragments into the competent cell,the library complexity could reach 109.Library mutation rate increased exponentially with the dilution and amplification of error-prone PCR.The error-prone PCR conditions were optimized including deoxyribonucleotide triphosphate(dNTP)concentration,Mn^(2+)concentration,Mg^(2+)concentration,PCR cycle number,and primer length.Then,a RNA library with high complexity can be obtained by in vitro transcription to meet most molecular biological screening requirements,and can also be used for mRNA vaccine screening.展开更多
A comprehensive study was presented for WLAN 802.11b using error-prone channel. It was theoretically and numerically evaluated the performance of three different network sizes with the bit rates that available in 802....A comprehensive study was presented for WLAN 802.11b using error-prone channel. It was theoretically and numerically evaluated the performance of three different network sizes with the bit rates that available in 802.11b protocol. Results show that throughput does not change with the size of the network for wide range of bit error rates (BERs) and the channel bit rates play a significant role in the main characteristics of the network. A comprehensive explanation has given for the phenomenon of the packet delay suppression at relatively high level of BERs in view of the size of the networks and the BERs. The effect length of the transmitting packets is also investigated.展开更多
In WLANs, stations sharing a common wireless channel are governed by IEEE 802.11 protocol. Many conscious studies have been conducted to utilize this precious medium efficiently. However, most of these studies have be...In WLANs, stations sharing a common wireless channel are governed by IEEE 802.11 protocol. Many conscious studies have been conducted to utilize this precious medium efficiently. However, most of these studies have been done either under assumption of idealistic channel condition or with unlimited retransmitting number. This paper is devoted to investigate influence of limited retransmissions and error level in the utilizing channel on the network throughput, probability of packet dropping and time to drop a packet. The results show that for networks using basic access mechanism the throughput is suppressed with increasing amount of errors in the transmitting channel over all the range of the retry limit. It is also quite sensitive to the size of the network. On the other side, the networks using four-way handshaking mechanism has a good immunity against the error over the available range of retry limits. Also the throughput is unchangeable with size of the network over the range of retransmission limits. However, the throughput does not change with retry limits when it exceeds the maximum number of the backoff stage in both DCF’s mechanisms. In both mechanisms the probability of dropping a packet is a decreasing function with number of retransmissions and the time to drop a packet in the queue of a station is a strong function to the number of retry limit, size of the network, the utilizing medium access mechanism and amount of errors in the channel.展开更多
Polymerase-tautomeric model for untargeted delayed base substitution mutations is proposed.Structural analysis of bases insertion showed that any canonical bases may be inserted opposite rare tautomeric forms of thymi...Polymerase-tautomeric model for untargeted delayed base substitution mutations is proposed.Structural analysis of bases insertion showed that any canonical bases may be inserted opposite rare tautomeric forms of thymine T3*,adenines A2*and A4*so that between them hydrogen bonds are formed.Canonical adenine and cytosine can be incorporated opposite canonical thymine only.Canonical thymine and guanine can be incorporated opposite canonical adenine only.If in the synthesis of DNA containing rare tautomeric forms of thymine T3*,adenines A2*and A4*,involved DNA polymerases with relatively high fidelity of synthesis,mutations not appear.However,if further DNA synthesis will involve DNA polymerases having a low fidelity of synthesis,there may be base substitution mutations.It was shown that the conclusion made in the Tomasetti and Vogelstein cancer risk model that the formation of about 67%of all mutations was not caused by exposure to any mutagens is erroneous.展开更多
In this paper, a new three-dimensional Markov model is proposed for the estimation of saturation throughput of RTS/CTS (request to send/clear to send) scheme in an error-prone wireless local area network (WLAN) channe...In this paper, a new three-dimensional Markov model is proposed for the estimation of saturation throughput of RTS/CTS (request to send/clear to send) scheme in an error-prone wireless local area network (WLAN) channel. The model takes account of the effect of bit error on all the frames, and station short and long retry limits. Saturation throughput was re-analyzed using the new Markov model and numerical results closely matched those from simulation, confirming the accuracy of the new model. Evaluation of the influence of different parameters on throughput showed that the saturation throughput is sensitive to channel bit error rates and packet length, especially in high bit error conditions.展开更多
In this paper,the estimation for a class of generalized varying coefficient models with error-prone covariates is considered.By combining basis function approximations with some auxiliary variables,an instrumental var...In this paper,the estimation for a class of generalized varying coefficient models with error-prone covariates is considered.By combining basis function approximations with some auxiliary variables,an instrumental variable type estimation procedure is proposed.The asymptotic results of the estimator,such as the consistency and the weak convergence rate,are obtained.The proposed procedure can attenuate the effect of measurement errors and have proved workable for finite samples.展开更多
This paper studies the estimation and inference for a class of varying-coefficient regression models with error-prone covariates.The authors focus on the situation where the covariates are unobserved,there are no repe...This paper studies the estimation and inference for a class of varying-coefficient regression models with error-prone covariates.The authors focus on the situation where the covariates are unobserved,there are no repeated measurements,and the covariance matrix of the measurement errors is unknown,but some auxiliary information is available.The authors propose an instrumental variable type local polynomial estimator for the unknown varying-coefficient functions,and show that the estimator achieves the optimal nonparametric convergence rate,is asymptotically normal,and avoids using undersmoothing to allow the bandwidths to be selected using data-driven methods.A simulation is carried out to study the finite sample performance of the proposed estimator,and a real date set is analyzed to illustrate the usefulness of the developed methodology.展开更多
基金Shanghai Science and Technology Commission’s“Belt and Road Initiative”International Cooperation Project,China(No.19410741800)。
文摘Library construction is a common method used to screen target genes in molecular biology.Most library constructions are not suitable for a small DNA library(<100 base pair(bp))and low RNA library output.To maximize the library’s complexity,error-prone polymerase chain reaction(PCR)was used to increase the base mutation rate.After introducing the DNA fragments into the competent cell,the library complexity could reach 109.Library mutation rate increased exponentially with the dilution and amplification of error-prone PCR.The error-prone PCR conditions were optimized including deoxyribonucleotide triphosphate(dNTP)concentration,Mn^(2+)concentration,Mg^(2+)concentration,PCR cycle number,and primer length.Then,a RNA library with high complexity can be obtained by in vitro transcription to meet most molecular biological screening requirements,and can also be used for mRNA vaccine screening.
文摘A comprehensive study was presented for WLAN 802.11b using error-prone channel. It was theoretically and numerically evaluated the performance of three different network sizes with the bit rates that available in 802.11b protocol. Results show that throughput does not change with the size of the network for wide range of bit error rates (BERs) and the channel bit rates play a significant role in the main characteristics of the network. A comprehensive explanation has given for the phenomenon of the packet delay suppression at relatively high level of BERs in view of the size of the networks and the BERs. The effect length of the transmitting packets is also investigated.
文摘In WLANs, stations sharing a common wireless channel are governed by IEEE 802.11 protocol. Many conscious studies have been conducted to utilize this precious medium efficiently. However, most of these studies have been done either under assumption of idealistic channel condition or with unlimited retransmitting number. This paper is devoted to investigate influence of limited retransmissions and error level in the utilizing channel on the network throughput, probability of packet dropping and time to drop a packet. The results show that for networks using basic access mechanism the throughput is suppressed with increasing amount of errors in the transmitting channel over all the range of the retry limit. It is also quite sensitive to the size of the network. On the other side, the networks using four-way handshaking mechanism has a good immunity against the error over the available range of retry limits. Also the throughput is unchangeable with size of the network over the range of retransmission limits. However, the throughput does not change with retry limits when it exceeds the maximum number of the backoff stage in both DCF’s mechanisms. In both mechanisms the probability of dropping a packet is a decreasing function with number of retransmissions and the time to drop a packet in the queue of a station is a strong function to the number of retry limit, size of the network, the utilizing medium access mechanism and amount of errors in the channel.
文摘Polymerase-tautomeric model for untargeted delayed base substitution mutations is proposed.Structural analysis of bases insertion showed that any canonical bases may be inserted opposite rare tautomeric forms of thymine T3*,adenines A2*and A4*so that between them hydrogen bonds are formed.Canonical adenine and cytosine can be incorporated opposite canonical thymine only.Canonical thymine and guanine can be incorporated opposite canonical adenine only.If in the synthesis of DNA containing rare tautomeric forms of thymine T3*,adenines A2*and A4*,involved DNA polymerases with relatively high fidelity of synthesis,mutations not appear.However,if further DNA synthesis will involve DNA polymerases having a low fidelity of synthesis,there may be base substitution mutations.It was shown that the conclusion made in the Tomasetti and Vogelstein cancer risk model that the formation of about 67%of all mutations was not caused by exposure to any mutagens is erroneous.
文摘In this paper, a new three-dimensional Markov model is proposed for the estimation of saturation throughput of RTS/CTS (request to send/clear to send) scheme in an error-prone wireless local area network (WLAN) channel. The model takes account of the effect of bit error on all the frames, and station short and long retry limits. Saturation throughput was re-analyzed using the new Markov model and numerical results closely matched those from simulation, confirming the accuracy of the new model. Evaluation of the influence of different parameters on throughput showed that the saturation throughput is sensitive to channel bit error rates and packet length, especially in high bit error conditions.
基金Supported by the National Natural Science Foundation of China(11101119)the Natural Science Foundation of Guangxi(2010GXNSFB013051)the Philosophy and Social Sciences Foundation of Guangxi(11FTJ002)
文摘In this paper,the estimation for a class of generalized varying coefficient models with error-prone covariates is considered.By combining basis function approximations with some auxiliary variables,an instrumental variable type estimation procedure is proposed.The asymptotic results of the estimator,such as the consistency and the weak convergence rate,are obtained.The proposed procedure can attenuate the effect of measurement errors and have proved workable for finite samples.
基金supported by the Graduate Student Innovation Foundation of SHUFE(#CXJJ-2011-351)supported by the Natural Sciences and Engineering Research Council of Canada
文摘This paper studies the estimation and inference for a class of varying-coefficient regression models with error-prone covariates.The authors focus on the situation where the covariates are unobserved,there are no repeated measurements,and the covariance matrix of the measurement errors is unknown,but some auxiliary information is available.The authors propose an instrumental variable type local polynomial estimator for the unknown varying-coefficient functions,and show that the estimator achieves the optimal nonparametric convergence rate,is asymptotically normal,and avoids using undersmoothing to allow the bandwidths to be selected using data-driven methods.A simulation is carried out to study the finite sample performance of the proposed estimator,and a real date set is analyzed to illustrate the usefulness of the developed methodology.
