Extract of the eschar tissue taken from the rat which had been given full-thickness burn of20% TBSA was found to be inhibitory to the respiratory function of the mitochondria and theenergization of the inner membrane....Extract of the eschar tissue taken from the rat which had been given full-thickness burn of20% TBSA was found to be inhibitory to the respiratory function of the mitochondria and theenergization of the inner membrane. The inhibition was also demonstrated by the 1: 2 and 1: 4and 1: 8 dilutions of the eschar extract. The extract of normal skin only showed weak inhibitory cf-fect. The 1: 4 dilution of normal skin had no (?) on mitochondrial functions. There is a widegap between the malonaldehyde (MDA) contents of these two kinds of extracts. An apparent posi-tive correlation was found between MDA content and degree of inhibition. However, on close ex-amination, it was found that the MDA content is not the direct cause of the decrease of therespiratory control ratio (RCR). The increase of MDA content is probably related to the destruc-tion of the lipid bilayer of the mitochondria. Data from the energization experiment show that theenergization process is compromised when the destruction of the membrane has proceeded to a cer-tain degrce. The inhibition of mitochondrial respiratory function becomes evident when encrgizationfunction is still normal. Therefore at least two mechanisms are operating in the inhibition process.Further work is needed to elucidate the problem.展开更多
The effects of toxic substance D1,extracted from burn eschars,on the hepatic mitochondrial respiratory function and its damages to the respirtatory chain were observed in the rat.It was found that the rate of stste 3r...The effects of toxic substance D1,extracted from burn eschars,on the hepatic mitochondrial respiratory function and its damages to the respirtatory chain were observed in the rat.It was found that the rate of stste 3respiration was inhibited and that of state 4 respiration stimulated by D1.There was an obvious dosage-effect relationship between D1 dosage and the rate of oxygen consumption, that is,the changes of the rate was slow in the range of low dosage and fast in the range of high dosage. The point of change was at 1 mg/ml of D1 concentration.The ratio of FCCP-stimulated respiration rate/state 4 respiration rate was decreased when D1 dosage was increased.The results of experiments were similar whether glutamate+malate or succinate were used as the substrate. The point of change was also at 1 mg/ml of D1 concentration. The decrease of FCCP-stimulated rate/state 4rate implied that the respiratory chain was damaged.But complex Ⅳ-the terminal segment of the chain-remained intact. Thus, the site of damage must be somewhere in complexesⅠ￣Ⅲ and/or complexes Ⅱ￣Ⅲ. Since NADH dehydrogenase and succinate dehydrogenase-the main enzymes in complexesⅠand Ⅱ-were not affected by D1 but NADH: cytochrome c reductase and succinate:cytochrome c reductase markedly inhibited, the site of damage was most likely located in complex Ⅲ-the bc1 complex.展开更多
The effects of D1 , an extract of burn eschar , and D'1 , an extract of normal skin of rats , on the induction of chemiluminescense in heparocytic submitochondrial particles were studied. It was found that both D1...The effects of D1 , an extract of burn eschar , and D'1 , an extract of normal skin of rats , on the induction of chemiluminescense in heparocytic submitochondrial particles were studied. It was found that both D1 and D'1 were able to induce chemiluminescence in hepatocytic submitochondrial particles in a dosage-dependent manner and D1 was more potent than D'1 in this function when they were in the same dosage. When superoxide dismutase (SOD) and beta-carotene were in dosage smaller than 10μg/ml and 3. 2 mmol/L respectively, they were able to suppress the D1's capacity to induce chemiluminescence in the submitochondrial particles in a dosage-dependent manner , too. These findings suggest that there is the occurrence of free radicals in the interaction between D1' and submitochondrial particles and superoxide anions and siglet oxygen seem to be involved.展开更多
Mediterranean spotted fever(MSF)has been diagnosed clinically in the Crimean Peninsula since the 1930s.We describe the recent illness of an elderly patient from Crimea who had developed a classic triad of MSF symptoms...Mediterranean spotted fever(MSF)has been diagnosed clinically in the Crimean Peninsula since the 1930s.We describe the recent illness of an elderly patient from Crimea who had developed a classic triad of MSF symptoms consisting of fever,maculopapular rash,and eschar.Clinical diagnosis of rickettsiosis was confirmed using real-time PCR and sequencing of 4 Rickettsia protein genes.The strain causing clinical illness was characterized as Rickettsia conorii subspecies conorii Malish 7.This report corroborates the utility of eschar swab material as a source of DNA for PCR-based diagnostics that enables timely patient treatment and management.展开更多
BACKGROUND Scrub typhus is a naturally occurring acute infectious disease that is primarily transmitted through the bites of chiggers or larval mites infected by Orientia tsutsugamushi(O.tsutsugamushi).Omadacycline,a ...BACKGROUND Scrub typhus is a naturally occurring acute infectious disease that is primarily transmitted through the bites of chiggers or larval mites infected by Orientia tsutsugamushi(O.tsutsugamushi).Omadacycline,a novel tetracycline,exhibits potent antibacterial efficacy against both typical bacteria and atypical pathogens.However,omadacycline application in the treatment of scrub typhus remains limited.CASE SUMMARY In the present work,we report several cases of scrub typhus,with the main clinical symptoms being fever,the formation of eschars or ulcers,local or systemic lymphadenopathy,headache,myalgia and rash.Blood samples were collected before omadacycline was administered,and O.tsutsugamushi infection was confirmed through targeted next-generation sequencing(tNGS).After two days of treatment,the patients’symptoms,including fever,were alleviated,with no adverse drug reactions.CONCLUSION tNGS is an effective method for diagnosing scrub typhus.Omadacycline can be considered an alternative option for antiinfective therapy in patients with O.tsutsugamushi infections.展开更多
目的探讨右美托咪定对大面积烧伤患者静脉全身麻醉（全麻）切痂植皮术应激反应的影响。方法选择择期行大面积烧伤削痂植皮术患者60例,ASAⅠ、Ⅱ级,随机均分成生理盐水对照组（S组）和右美托咪定组（D组）。诱导前10 min D组静脉泵注负...目的探讨右美托咪定对大面积烧伤患者静脉全身麻醉（全麻）切痂植皮术应激反应的影响。方法选择择期行大面积烧伤削痂植皮术患者60例,ASAⅠ、Ⅱ级,随机均分成生理盐水对照组（S组）和右美托咪定组（D组）。诱导前10 min D组静脉泵注负荷量的右美托咪定（1μg/kg,10 min）。泵完以0.5μg/（kg·h）的右美托咪定维持,S组泵注相同剂量的生理盐水。两组均以氯胺酮1.0mg/kg,丙泊酚1.0mg/kg静注诱导,氯胺酮2.0-4.0mg/（kg·h）泵注复合丙泊酚2-4μg/mL靶控输注维持麻醉深度,使患者BIS值维持在46-55。观察两组诱导前（T0）、诱导后（T1）,削痂（T2）、取皮（T3）、植皮（T4）、手术结束（T5）各时间点MAP、HR变化及测定血浆肾上腺素（Epinephrine,E）、去甲肾上腺素（Norepinephrine,NE）、皮质醇（Cortisol,COR）、血糖（Glucose,GLU）含量。记录从停药到意识恢复时间、出现呼吸抑制患者例数及苏醒期发生恶心呕吐、躁动患者例数。结果与T0时相比较,S组在T2-T5时HR明显增快,MAP明显增高（P〈0.05）,且均高于D组（P〈0.05）,而D组在T0-T5HR、MAP无明显变化（P〉0.05）。在T2-T5时,S组的E、NE、COR、GLU明显高于T0时和D组（P〈0.05）。两组的意识恢复时间,出现呼吸抑制患者例数无明显差异。但是,S组术后恶心呕吐、躁动的发生率明显高于D组（P〈0.05）。结论右美托咪定可降低大面积烧伤患者静脉全麻切痂植皮术的应激反应,并且可以较少相关并发症。展开更多
文摘Extract of the eschar tissue taken from the rat which had been given full-thickness burn of20% TBSA was found to be inhibitory to the respiratory function of the mitochondria and theenergization of the inner membrane. The inhibition was also demonstrated by the 1: 2 and 1: 4and 1: 8 dilutions of the eschar extract. The extract of normal skin only showed weak inhibitory cf-fect. The 1: 4 dilution of normal skin had no (?) on mitochondrial functions. There is a widegap between the malonaldehyde (MDA) contents of these two kinds of extracts. An apparent posi-tive correlation was found between MDA content and degree of inhibition. However, on close ex-amination, it was found that the MDA content is not the direct cause of the decrease of therespiratory control ratio (RCR). The increase of MDA content is probably related to the destruc-tion of the lipid bilayer of the mitochondria. Data from the energization experiment show that theenergization process is compromised when the destruction of the membrane has proceeded to a cer-tain degrce. The inhibition of mitochondrial respiratory function becomes evident when encrgizationfunction is still normal. Therefore at least two mechanisms are operating in the inhibition process.Further work is needed to elucidate the problem.
文摘The effects of toxic substance D1,extracted from burn eschars,on the hepatic mitochondrial respiratory function and its damages to the respirtatory chain were observed in the rat.It was found that the rate of stste 3respiration was inhibited and that of state 4 respiration stimulated by D1.There was an obvious dosage-effect relationship between D1 dosage and the rate of oxygen consumption, that is,the changes of the rate was slow in the range of low dosage and fast in the range of high dosage. The point of change was at 1 mg/ml of D1 concentration.The ratio of FCCP-stimulated respiration rate/state 4 respiration rate was decreased when D1 dosage was increased.The results of experiments were similar whether glutamate+malate or succinate were used as the substrate. The point of change was also at 1 mg/ml of D1 concentration. The decrease of FCCP-stimulated rate/state 4rate implied that the respiratory chain was damaged.But complex Ⅳ-the terminal segment of the chain-remained intact. Thus, the site of damage must be somewhere in complexesⅠ￣Ⅲ and/or complexes Ⅱ￣Ⅲ. Since NADH dehydrogenase and succinate dehydrogenase-the main enzymes in complexesⅠand Ⅱ-were not affected by D1 but NADH: cytochrome c reductase and succinate:cytochrome c reductase markedly inhibited, the site of damage was most likely located in complex Ⅲ-the bc1 complex.
文摘The effects of D1 , an extract of burn eschar , and D'1 , an extract of normal skin of rats , on the induction of chemiluminescense in heparocytic submitochondrial particles were studied. It was found that both D1 and D'1 were able to induce chemiluminescence in hepatocytic submitochondrial particles in a dosage-dependent manner and D1 was more potent than D'1 in this function when they were in the same dosage. When superoxide dismutase (SOD) and beta-carotene were in dosage smaller than 10μg/ml and 3. 2 mmol/L respectively, they were able to suppress the D1's capacity to induce chemiluminescence in the submitochondrial particles in a dosage-dependent manner , too. These findings suggest that there is the occurrence of free radicals in the interaction between D1' and submitochondrial particles and superoxide anions and siglet oxygen seem to be involved.
文摘Mediterranean spotted fever(MSF)has been diagnosed clinically in the Crimean Peninsula since the 1930s.We describe the recent illness of an elderly patient from Crimea who had developed a classic triad of MSF symptoms consisting of fever,maculopapular rash,and eschar.Clinical diagnosis of rickettsiosis was confirmed using real-time PCR and sequencing of 4 Rickettsia protein genes.The strain causing clinical illness was characterized as Rickettsia conorii subspecies conorii Malish 7.This report corroborates the utility of eschar swab material as a source of DNA for PCR-based diagnostics that enables timely patient treatment and management.
基金Supported by National Natural Science Foundation of China,No.81800721the Postdoctoral Foundation of China,2020M671387.
文摘BACKGROUND Scrub typhus is a naturally occurring acute infectious disease that is primarily transmitted through the bites of chiggers or larval mites infected by Orientia tsutsugamushi(O.tsutsugamushi).Omadacycline,a novel tetracycline,exhibits potent antibacterial efficacy against both typical bacteria and atypical pathogens.However,omadacycline application in the treatment of scrub typhus remains limited.CASE SUMMARY In the present work,we report several cases of scrub typhus,with the main clinical symptoms being fever,the formation of eschars or ulcers,local or systemic lymphadenopathy,headache,myalgia and rash.Blood samples were collected before omadacycline was administered,and O.tsutsugamushi infection was confirmed through targeted next-generation sequencing(tNGS).After two days of treatment,the patients’symptoms,including fever,were alleviated,with no adverse drug reactions.CONCLUSION tNGS is an effective method for diagnosing scrub typhus.Omadacycline can be considered an alternative option for antiinfective therapy in patients with O.tsutsugamushi infections.
文摘目的探讨右美托咪定对大面积烧伤患者静脉全身麻醉（全麻）切痂植皮术应激反应的影响。方法选择择期行大面积烧伤削痂植皮术患者60例,ASAⅠ、Ⅱ级,随机均分成生理盐水对照组（S组）和右美托咪定组（D组）。诱导前10 min D组静脉泵注负荷量的右美托咪定（1μg/kg,10 min）。泵完以0.5μg/（kg·h）的右美托咪定维持,S组泵注相同剂量的生理盐水。两组均以氯胺酮1.0mg/kg,丙泊酚1.0mg/kg静注诱导,氯胺酮2.0-4.0mg/（kg·h）泵注复合丙泊酚2-4μg/mL靶控输注维持麻醉深度,使患者BIS值维持在46-55。观察两组诱导前（T0）、诱导后（T1）,削痂（T2）、取皮（T3）、植皮（T4）、手术结束（T5）各时间点MAP、HR变化及测定血浆肾上腺素（Epinephrine,E）、去甲肾上腺素（Norepinephrine,NE）、皮质醇（Cortisol,COR）、血糖（Glucose,GLU）含量。记录从停药到意识恢复时间、出现呼吸抑制患者例数及苏醒期发生恶心呕吐、躁动患者例数。结果与T0时相比较,S组在T2-T5时HR明显增快,MAP明显增高（P〈0.05）,且均高于D组（P〈0.05）,而D组在T0-T5HR、MAP无明显变化（P〉0.05）。在T2-T5时,S组的E、NE、COR、GLU明显高于T0时和D组（P〈0.05）。两组的意识恢复时间,出现呼吸抑制患者例数无明显差异。但是,S组术后恶心呕吐、躁动的发生率明显高于D组（P〈0.05）。结论右美托咪定可降低大面积烧伤患者静脉全麻切痂植皮术的应激反应,并且可以较少相关并发症。
