In vitro organogenesis of an upland species of Colocasia esculenta cv. antiquorum L. was examined in relation to different explants like meristem and parenchymatous storage tissues with or without anthocyanin layer, f...In vitro organogenesis of an upland species of Colocasia esculenta cv. antiquorum L. was examined in relation to different explants like meristem and parenchymatous storage tissues with or without anthocyanin layer, four levels of each of Kn, 2,4-D, NAA and BAP and four incubation environments such as: 1) 16 h 3 Kl light intensity + 24°C ± 2°C;2) 24 h dark + 24°C ± 2°C;3) 24 h dark + 30°C ± 3°C and 4) 12 h diffuse light + 30°C ± 3°C. Only meristems showed proliferation with various degree of intensity both at 16 h 3 Kl light + 24°C ± 2°C and 24 h dark + 24°C ± 2°C conditions and poor response with different levels of Kn + NAA either in light or in the dark. Cultures with NAA + BAP were proliferated very quickly with very high degree of intensity. The cultures under dark did not proliferate for 20 days which upon transfer to light showed high degree of proliferation. Cultures with NAA + BAP formed calluses more pronouncedly at dark than that occurred in the light. Parenchymatous tissues with or without anthocyanin did not proliferate but the tissues with anthocyanin lost pigmentation after 25 - 30 days and turned to grey colour after 50 days while tissues without anthocyanin turned to green colour with shinny pimples indicating that protocorm may be developed. No culture under high temperature environment (30°C ± 3°C) neither survived nor proliferated. The meristems in culture were died within 15 - 20 days while others within 25-30 days. In conclusion, a combination of NAA (0.5 - 3.0 mg/l) and BAP (0.5 - 2.0 mg/l) and an incubation photoperiod of 16 h coupled with temperature of 24°C ± 2°C were found most suitable for in vitro culture of Colocasia esculenta cv. antiquorum L.展开更多
Taro （Colocasia esculenta L. Schott） is an important underutilised crop in South Africa, East Africa and Indonesia. Three taro landraces, namely, Dumbe Lomfula （wild）, KwaNgwanase and Umbumbulu, were collected fro...Taro （Colocasia esculenta L. Schott） is an important underutilised crop in South Africa, East Africa and Indonesia. Three taro landraces, namely, Dumbe Lomfula （wild）, KwaNgwanase and Umbumbulu, were collected from two locations in KwaZulu-Natal （KZN）, South Africa, and planted at two locations, Pietermaritzburg （KZN） and Roodeplaat, Pretoria. Ago-morphological characterisation of vegetative and corm characteristics were done four months after planting and at harvest, respectively. Sampling for DNA fingerprinting using five SSR primers was done using leaf material four months after planting. Agro-morphological characterisation was useful in showing differences between the wild landrace and the two cultivated landraces, as well as identification of dasheen and eddoe types. SSR primer characterisation showed that despite significant morphological difference, the wild Dumbe Lomfula and Umbumbulu landraces were closely related but different from the KwaNgwanase landrace. Although landraces showed great morphological variation, this did not necessarily imply genetic variation. It is concluded that SSR primers are more useful for characterising taro landraces.展开更多
A traditional process used by farmers in Chad consists in soaking slices of taro (Colocasia esculenta L. SCHOTT) in tamarind infusion, or in corn solution or in water over a 24-hour period to reduce the acridity of ta...A traditional process used by farmers in Chad consists in soaking slices of taro (Colocasia esculenta L. SCHOTT) in tamarind infusion, or in corn solution or in water over a 24-hour period to reduce the acridity of taro and facilitate cooking. The aim of this study was to assess the effect of traditional soaking on the in vitro digestibility of taro flour using or not using an α-amylase enzyme. The digestion without the enzyme has shown that the soaking processes improve the digestibility of taro flour (from 39.30% for the control sample to 75.11% (after tamarind infusion) and 78.67% (treatment with water) after 24 hours of soaking). Soaking over a 6-hour period and preferentially in tamarind infusion or in corn solution obtains highly digestible flour (around 95% of digestibility rate after 3 hours of enzymatic digestion).展开更多
Cotton growth and development are determined and influenced by cultivars, meteorological conditions, and management practices. The objective of this study was to quantify the optimum of temperature-light meteorologica...Cotton growth and development are determined and influenced by cultivars, meteorological conditions, and management practices. The objective of this study was to quantify the optimum of temperature-light meteorological factors for seedcotton biomass per boll with respect to boll positions. Field experiments were conducted using two cultivars of Kemian 1 and Sumian 15 with three planting dates of 25 April （mean daily temperature （MDT） was 28.0 and 25.4°C in 2010 and 2011, respectively）, 25 May （MDT was 22.5 and 21.2°C in 2010 and 2011, respectively）, and 10 Jun （MDT was 18.7 and 17.9°C in 2010 and 2011, respectively）, and under three shading levels （crop relative light rates （CRLR） were 100, 80, and 60％） during 2010 and 2011 cotton boll development period （from anthesis to boll open stages）. The main meteorological factors （temperature and light） affected seedcotton biomass per boll differently among different boll positions and cultivars. Mean daily radiation （MDR） affected seedcotton biomass per boll at all boll positions, except fruiting branch 2 （FB2） fruting node 1 （FN1）. However, its influence was less than temperature factors, especially growing degree-days （GDD）. Optimum mean daily maximum temperature （MDTmax） for seedcotton biomass per boll at FB11FN3 was 29.9-32.4°C, and the optimum MDR at aforementioned position was 15.8-17.5 MJ m-2. Definitely, these results can contribute to future cultural practices such as rational cultivars choice and distribution, simplifying field managements and mechanization to acquire more efficient and economical cotton management.展开更多
qSsnps-5D为一个控制不育小穗数的主效稳定QTL,其优异等位基因来自小麦骨干亲本京411。本研究利用科农9204×京411衍生的包含187个家系的重组自交系群体(KJ-RIL,recombinant inbred lines derived from the cross of Kenong 9204 an...qSsnps-5D为一个控制不育小穗数的主效稳定QTL,其优异等位基因来自小麦骨干亲本京411。本研究利用科农9204×京411衍生的包含187个家系的重组自交系群体(KJ-RIL,recombinant inbred lines derived from the cross of Kenong 9204 and Jing 411)及314份育成品种(系)组成的自然群体对其进行遗传及育种选择效应解析,明确其对产量性状的遗传效应,分析其在育种过程中的选择应用情况,评价其未来育种应用潜力。试验结果表明,qSsnps-5D在8套数据集中被定位于5D染色体上0.72~4.13 Mb之间,跨度约3.41 Mb。基于KJ-RIL群体及自然群体分析结果均表明,来自京411的优异等位基因可增加单株穗数,但对千粒重表现为极显著负向效应;其对穗粒数、单株产量的影响在两套群体的分析结果不一致。在qSsnps-5D靶区间内选择2个紧密连锁的SNP标记AX-110565536和AX-86170796对314份自然群体进行目标QTL单倍型分析;结果显示,国外品种对qSsnps-5D优异单倍型(Hap-GG-CC)的选择利用率最高;中国品种中青海省、四川省和河南省3个省份优异单倍型品种占比较高,而山东、北京、陕西和河北4地对qSsnps-5D优异单倍型选择利用率较低。时间跨度显示,qSsnps-5D优异单倍型Hap-GG-CC选择利用效率随时间推移在我国呈下降趋势。为便于qSsnps-5D后期分子育种应用,本研究开发了一个基于PCR检测技术的InDel分子标记,命名为5D-1620921,其带型扩增清晰,可重复性好,为qSsnps-5D分子育种应用提供理论支撑。展开更多
文摘In vitro organogenesis of an upland species of Colocasia esculenta cv. antiquorum L. was examined in relation to different explants like meristem and parenchymatous storage tissues with or without anthocyanin layer, four levels of each of Kn, 2,4-D, NAA and BAP and four incubation environments such as: 1) 16 h 3 Kl light intensity + 24°C ± 2°C;2) 24 h dark + 24°C ± 2°C;3) 24 h dark + 30°C ± 3°C and 4) 12 h diffuse light + 30°C ± 3°C. Only meristems showed proliferation with various degree of intensity both at 16 h 3 Kl light + 24°C ± 2°C and 24 h dark + 24°C ± 2°C conditions and poor response with different levels of Kn + NAA either in light or in the dark. Cultures with NAA + BAP were proliferated very quickly with very high degree of intensity. The cultures under dark did not proliferate for 20 days which upon transfer to light showed high degree of proliferation. Cultures with NAA + BAP formed calluses more pronouncedly at dark than that occurred in the light. Parenchymatous tissues with or without anthocyanin did not proliferate but the tissues with anthocyanin lost pigmentation after 25 - 30 days and turned to grey colour after 50 days while tissues without anthocyanin turned to green colour with shinny pimples indicating that protocorm may be developed. No culture under high temperature environment (30°C ± 3°C) neither survived nor proliferated. The meristems in culture were died within 15 - 20 days while others within 25-30 days. In conclusion, a combination of NAA (0.5 - 3.0 mg/l) and BAP (0.5 - 2.0 mg/l) and an incubation photoperiod of 16 h coupled with temperature of 24°C ± 2°C were found most suitable for in vitro culture of Colocasia esculenta cv. antiquorum L.
文摘Taro （Colocasia esculenta L. Schott） is an important underutilised crop in South Africa, East Africa and Indonesia. Three taro landraces, namely, Dumbe Lomfula （wild）, KwaNgwanase and Umbumbulu, were collected from two locations in KwaZulu-Natal （KZN）, South Africa, and planted at two locations, Pietermaritzburg （KZN） and Roodeplaat, Pretoria. Ago-morphological characterisation of vegetative and corm characteristics were done four months after planting and at harvest, respectively. Sampling for DNA fingerprinting using five SSR primers was done using leaf material four months after planting. Agro-morphological characterisation was useful in showing differences between the wild landrace and the two cultivated landraces, as well as identification of dasheen and eddoe types. SSR primer characterisation showed that despite significant morphological difference, the wild Dumbe Lomfula and Umbumbulu landraces were closely related but different from the KwaNgwanase landrace. Although landraces showed great morphological variation, this did not necessarily imply genetic variation. It is concluded that SSR primers are more useful for characterising taro landraces.
基金grateful to Chad French Ambassy for the financing of this project.
文摘A traditional process used by farmers in Chad consists in soaking slices of taro (Colocasia esculenta L. SCHOTT) in tamarind infusion, or in corn solution or in water over a 24-hour period to reduce the acridity of taro and facilitate cooking. The aim of this study was to assess the effect of traditional soaking on the in vitro digestibility of taro flour using or not using an α-amylase enzyme. The digestion without the enzyme has shown that the soaking processes improve the digestibility of taro flour (from 39.30% for the control sample to 75.11% (after tamarind infusion) and 78.67% (treatment with water) after 24 hours of soaking). Soaking over a 6-hour period and preferentially in tamarind infusion or in corn solution obtains highly digestible flour (around 95% of digestibility rate after 3 hours of enzymatic digestion).
基金supported by the National Natural Science Foundation of China (31471444,31401327)the Special Fund for Agro-scientific Research in the Public Interest of China (Impact of Climate Change on Agriculture Production,201203096)the Jiangsu Overseas Research and Training Program for University Prominent Young and Middle-aged Teachers and President,China (2016)
文摘Cotton growth and development are determined and influenced by cultivars, meteorological conditions, and management practices. The objective of this study was to quantify the optimum of temperature-light meteorological factors for seedcotton biomass per boll with respect to boll positions. Field experiments were conducted using two cultivars of Kemian 1 and Sumian 15 with three planting dates of 25 April （mean daily temperature （MDT） was 28.0 and 25.4°C in 2010 and 2011, respectively）, 25 May （MDT was 22.5 and 21.2°C in 2010 and 2011, respectively）, and 10 Jun （MDT was 18.7 and 17.9°C in 2010 and 2011, respectively）, and under three shading levels （crop relative light rates （CRLR） were 100, 80, and 60％） during 2010 and 2011 cotton boll development period （from anthesis to boll open stages）. The main meteorological factors （temperature and light） affected seedcotton biomass per boll differently among different boll positions and cultivars. Mean daily radiation （MDR） affected seedcotton biomass per boll at all boll positions, except fruiting branch 2 （FB2） fruting node 1 （FN1）. However, its influence was less than temperature factors, especially growing degree-days （GDD）. Optimum mean daily maximum temperature （MDTmax） for seedcotton biomass per boll at FB11FN3 was 29.9-32.4°C, and the optimum MDR at aforementioned position was 15.8-17.5 MJ m-2. Definitely, these results can contribute to future cultural practices such as rational cultivars choice and distribution, simplifying field managements and mechanization to acquire more efficient and economical cotton management.
文摘qSsnps-5D为一个控制不育小穗数的主效稳定QTL,其优异等位基因来自小麦骨干亲本京411。本研究利用科农9204×京411衍生的包含187个家系的重组自交系群体(KJ-RIL,recombinant inbred lines derived from the cross of Kenong 9204 and Jing 411)及314份育成品种(系)组成的自然群体对其进行遗传及育种选择效应解析,明确其对产量性状的遗传效应,分析其在育种过程中的选择应用情况,评价其未来育种应用潜力。试验结果表明,qSsnps-5D在8套数据集中被定位于5D染色体上0.72~4.13 Mb之间,跨度约3.41 Mb。基于KJ-RIL群体及自然群体分析结果均表明,来自京411的优异等位基因可增加单株穗数,但对千粒重表现为极显著负向效应;其对穗粒数、单株产量的影响在两套群体的分析结果不一致。在qSsnps-5D靶区间内选择2个紧密连锁的SNP标记AX-110565536和AX-86170796对314份自然群体进行目标QTL单倍型分析;结果显示,国外品种对qSsnps-5D优异单倍型(Hap-GG-CC)的选择利用率最高;中国品种中青海省、四川省和河南省3个省份优异单倍型品种占比较高,而山东、北京、陕西和河北4地对qSsnps-5D优异单倍型选择利用率较低。时间跨度显示,qSsnps-5D优异单倍型Hap-GG-CC选择利用效率随时间推移在我国呈下降趋势。为便于qSsnps-5D后期分子育种应用,本研究开发了一个基于PCR检测技术的InDel分子标记,命名为5D-1620921,其带型扩增清晰,可重复性好,为qSsnps-5D分子育种应用提供理论支撑。
