In this study, essential oils from Cymbopogon citratus and Ceasalpinia decapitala leaves, Citrus lemon and Citrus sinens＆ peels, were extracted, chemically characterized and evaluated on both Gram positive and Gram n...In this study, essential oils from Cymbopogon citratus and Ceasalpinia decapitala leaves, Citrus lemon and Citrus sinens＆ peels, were extracted, chemically characterized and evaluated on both Gram positive and Gram negative bacteria, Salmonella Typhimurium, Escherichia coli, Listeria innocua, Staphylococcus aureus, Klebsiella pneumonia and Streptococcus pyogenes. Essential oil of C. citratus was found to be rich in geranial （33.0%） and neral （49.7%）, that from C. decapitala in 13-pinene （8.4%）, ct-pinene （25.5%）, 13-ocymene （31.6%）, 15-phellandrene （8.4%）, caryophyllene （7.5%） and geranial （5.9%）. The essential oil from C. lemon and C. sinensis where mainly composed by limonene with percentages of 77.5% and 83.3% respectively, and 13-phellandrene with percentages of 8.1% and 10.8% for the essential oil of C. lemon and C. sinensis, respectively. The antibacterial activity assessment realized on different microorganisms contaminating food showed that essential oil from C. citratus was shah more effective against all tested bacteria while that from C. sinensis was the less active essential oil. The incorporation of essential oils in HPC （hydroxy-propyl cellulose） films showed that those essential oils, especially essential oils from C. citratus, maintained their antibacterial activities against targeted microorganisms and therefore can be applied in food packing.展开更多
In this work,active and edible films for food packaging were obtained by intensive mixing and compression molding of the wheat gluten(WG)glycerol-plasticized paste.Thyme essential oil(TEO,10 and 15 wt.%)was incorporat...In this work,active and edible films for food packaging were obtained by intensive mixing and compression molding of the wheat gluten(WG)glycerol-plasticized paste.Thyme essential oil(TEO,10 and 15 wt.%)was incorporated as the active component microencapsulated inβ-cyclodextrins(β-CD)and included directly into the biopolymer matrix for comparison.It was found that films incorporating microencapsulated TEO are more soluble in water(total soluble matter(dry method)of about 33%and 36.6%vs.22.4%and 18.6%,for films containing 10%and 15%TEO,respectively)but less rigid than those obtained with free oil(elastic tensile moduli of 6.9 and 3.1 vs.9.9 and 6.8 MPa,for films containing 10%and 15%TEO,respectively),although the water vapor permeability of the former is lower(4.9510−9 and 6.2910−9 vs.8.8510−9 and 11.1310−9 g/Pa*s*m,for films containing 10%and 15%TEO,respectively).Active films containing both free and encapsulated TEO inhibited gram(+)as well as gram(−)bacteria and exhibited enlarged antioxidant properties,with the latter presenting slightly better performance.In addition,encapsulation slowed the release rate of TEO from the films,allowing the bioactive to remain active in the film for much longer.Therefore,the present work revealed that films prepared from TEO/β-CD microcapsules included in compression-molded wheat gluten matrices showed great potential to be used as an active food packaging.展开更多
Fish skin gelatin films incorporated with various concentrations of cinnamon essential oil(CEO)were prepared and characterized.The results showed that tensile strength(TS),elongation at break(EAB),and water content(WC...Fish skin gelatin films incorporated with various concentrations of cinnamon essential oil(CEO)were prepared and characterized.The results showed that tensile strength(TS),elongation at break(EAB),and water content(WC)of the gelatin based film decreased with the increasing concentrations of CEO,but water vapor permeability(WVP)increased.Addition of CEO improved light barrier property of the film.The Scanning electron microscope(SEM)showed that the heterogeneous surface and porous formation appeared in gelatin-CEO films.Fourier transform infrared spectroscopy analyses(FTIR-ATR)spectra indicated the interactions existed between gelatin and CEO.The gelatin-CEO films exhibited good inhibitory effects against the tested microorganisms(Escherichia coli,Staphylococcus aureus,Aspergillus niger,Rhizopus oryzae,and Paecilomyces varioti)and their antifungal activity seemed to be more effective than the resistance to bacterial growth.In vitro release studies showed an initial burst effect of CEO release and that subsequently slowed down at 40℃,but the initial burst release was not obvious at 4℃.The obtained results suggested that incorporation of CEO as a natural antimicrobial agent into gelatin film has potential for developing as active food packaging.展开更多
Biocomposite films based on carboxymethyl cellulose(CMC)/polyvinyl alcohol(PVOH)added ginger essential oil(GEO)via Pickering emulsion(PE)method were fabricated and applied for preservation of bread.The films were asse...Biocomposite films based on carboxymethyl cellulose(CMC)/polyvinyl alcohol(PVOH)added ginger essential oil(GEO)via Pickering emulsion(PE)method were fabricated and applied for preservation of bread.The films were assessed for their physicochemical,morphological,mechanical,thermal,antioxidant,and microbial properties.SEM imaging indicated encapsulation of GEO spherical droplets into PE.The films exhibited lower tensile strength,higher elongation at break,and thickness after adition of GEO PE.ATR/FT-IR spectroscopy demonstrated the formation of some interaction between polymer matrix and GEO PE.Packaging of bread slices with GEO integrated biocomposite films extended the shelf life of bread from 4 to 30 days.Remarkably,the GEOloaded CMC/PVOH films showed excellent UV and light barrier properties,high antioxidant and antimicrobial activities,which make them suitable for food packaging application.展开更多
In this study,heat-denatured and alcalase-hydrolyzed whey and soy protein films/coatings containing marjoram essential oil(MEO)and thyme extract(TE)were investigated comparatively in terms of physicochemical and funct...In this study,heat-denatured and alcalase-hydrolyzed whey and soy protein films/coatings containing marjoram essential oil(MEO)and thyme extract(TE)were investigated comparatively in terms of physicochemical and functional properties.Heat-denaturated high protein solutions produced self-standing,smooth,transparent and viscoelastic films in the presence of glycerol.Enzymatic hydrolysis provided transparency,elasticity and extensibility to the films of low protein solutions.The addition of MEO and TE triggered hardness and brittleness especially in hydrolyzed high protein films and remarkably changed film color,thickness and viscosity possibly affecting surface adherence of films.Heat denatured films exhibited higher water vapor permeability(WVP)than alcalase-hydrolyzed ones.The lowest hardness was obtained in hydrolyzed low protein films with considerable adhesiveness.Hydrolyzed protein solutions coated to cheese provided better protection against moisture loss and MEO added films caused elevated hardening of cheese.MEO and TE incorporated in film matrices were seemed to suppress mycelial growth and sporulation of Aspergillus niger.This study presented valuable new findings on functional protein films produced by enzymatic hydrolysis as an alternative to heat treatment.展开更多
文摘In this study, essential oils from Cymbopogon citratus and Ceasalpinia decapitala leaves, Citrus lemon and Citrus sinens＆ peels, were extracted, chemically characterized and evaluated on both Gram positive and Gram negative bacteria, Salmonella Typhimurium, Escherichia coli, Listeria innocua, Staphylococcus aureus, Klebsiella pneumonia and Streptococcus pyogenes. Essential oil of C. citratus was found to be rich in geranial （33.0%） and neral （49.7%）, that from C. decapitala in 13-pinene （8.4%）, ct-pinene （25.5%）, 13-ocymene （31.6%）, 15-phellandrene （8.4%）, caryophyllene （7.5%） and geranial （5.9%）. The essential oil from C. lemon and C. sinensis where mainly composed by limonene with percentages of 77.5% and 83.3% respectively, and 13-phellandrene with percentages of 8.1% and 10.8% for the essential oil of C. lemon and C. sinensis, respectively. The antibacterial activity assessment realized on different microorganisms contaminating food showed that essential oil from C. citratus was shah more effective against all tested bacteria while that from C. sinensis was the less active essential oil. The incorporation of essential oils in HPC （hydroxy-propyl cellulose） films showed that those essential oils, especially essential oils from C. citratus, maintained their antibacterial activities against targeted microorganisms and therefore can be applied in food packing.
基金This study received financial support from the Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)(Grant#PIP 0855)the Agencia Nacional de Promoción de la Investigación,el Desarrollo Tecnológico y la Innovación(Agencia I+D+i)(Grant#PICT 03507)the Universidad Nacional de Mar del Plata(UNMdP)(Grant#22/G377).
文摘In this work,active and edible films for food packaging were obtained by intensive mixing and compression molding of the wheat gluten(WG)glycerol-plasticized paste.Thyme essential oil(TEO,10 and 15 wt.%)was incorporated as the active component microencapsulated inβ-cyclodextrins(β-CD)and included directly into the biopolymer matrix for comparison.It was found that films incorporating microencapsulated TEO are more soluble in water(total soluble matter(dry method)of about 33%and 36.6%vs.22.4%and 18.6%,for films containing 10%and 15%TEO,respectively)but less rigid than those obtained with free oil(elastic tensile moduli of 6.9 and 3.1 vs.9.9 and 6.8 MPa,for films containing 10%and 15%TEO,respectively),although the water vapor permeability of the former is lower(4.9510−9 and 6.2910−9 vs.8.8510−9 and 11.1310−9 g/Pa*s*m,for films containing 10%and 15%TEO,respectively).Active films containing both free and encapsulated TEO inhibited gram(+)as well as gram(−)bacteria and exhibited enlarged antioxidant properties,with the latter presenting slightly better performance.In addition,encapsulation slowed the release rate of TEO from the films,allowing the bioactive to remain active in the film for much longer.Therefore,the present work revealed that films prepared from TEO/β-CD microcapsules included in compression-molded wheat gluten matrices showed great potential to be used as an active food packaging.
基金This research was sponsored by the Education Scientific Research Foundation for Middle-aged and Young teachers of Education Department of Fujian Province(JAT160048)Opening Project of Fujian Provincial Engineering Technology Research Center of Marine Functional Food(C11178)Natural Science Foundation of Fujian Province(2015J01341,2016J01733).
文摘Fish skin gelatin films incorporated with various concentrations of cinnamon essential oil(CEO)were prepared and characterized.The results showed that tensile strength(TS),elongation at break(EAB),and water content(WC)of the gelatin based film decreased with the increasing concentrations of CEO,but water vapor permeability(WVP)increased.Addition of CEO improved light barrier property of the film.The Scanning electron microscope(SEM)showed that the heterogeneous surface and porous formation appeared in gelatin-CEO films.Fourier transform infrared spectroscopy analyses(FTIR-ATR)spectra indicated the interactions existed between gelatin and CEO.The gelatin-CEO films exhibited good inhibitory effects against the tested microorganisms(Escherichia coli,Staphylococcus aureus,Aspergillus niger,Rhizopus oryzae,and Paecilomyces varioti)and their antifungal activity seemed to be more effective than the resistance to bacterial growth.In vitro release studies showed an initial burst effect of CEO release and that subsequently slowed down at 40℃,but the initial burst release was not obvious at 4℃.The obtained results suggested that incorporation of CEO as a natural antimicrobial agent into gelatin film has potential for developing as active food packaging.
文摘Biocomposite films based on carboxymethyl cellulose(CMC)/polyvinyl alcohol(PVOH)added ginger essential oil(GEO)via Pickering emulsion(PE)method were fabricated and applied for preservation of bread.The films were assessed for their physicochemical,morphological,mechanical,thermal,antioxidant,and microbial properties.SEM imaging indicated encapsulation of GEO spherical droplets into PE.The films exhibited lower tensile strength,higher elongation at break,and thickness after adition of GEO PE.ATR/FT-IR spectroscopy demonstrated the formation of some interaction between polymer matrix and GEO PE.Packaging of bread slices with GEO integrated biocomposite films extended the shelf life of bread from 4 to 30 days.Remarkably,the GEOloaded CMC/PVOH films showed excellent UV and light barrier properties,high antioxidant and antimicrobial activities,which make them suitable for food packaging application.
文摘In this study,heat-denatured and alcalase-hydrolyzed whey and soy protein films/coatings containing marjoram essential oil(MEO)and thyme extract(TE)were investigated comparatively in terms of physicochemical and functional properties.Heat-denaturated high protein solutions produced self-standing,smooth,transparent and viscoelastic films in the presence of glycerol.Enzymatic hydrolysis provided transparency,elasticity and extensibility to the films of low protein solutions.The addition of MEO and TE triggered hardness and brittleness especially in hydrolyzed high protein films and remarkably changed film color,thickness and viscosity possibly affecting surface adherence of films.Heat denatured films exhibited higher water vapor permeability(WVP)than alcalase-hydrolyzed ones.The lowest hardness was obtained in hydrolyzed low protein films with considerable adhesiveness.Hydrolyzed protein solutions coated to cheese provided better protection against moisture loss and MEO added films caused elevated hardening of cheese.MEO and TE incorporated in film matrices were seemed to suppress mycelial growth and sporulation of Aspergillus niger.This study presented valuable new findings on functional protein films produced by enzymatic hydrolysis as an alternative to heat treatment.
