Eco-physiological characteristics of Tetracentron sinense Oliv.saplings in response to different light intensities

The regeneration of Tetracentron sinense Oliv.is poor in the understory and in open areas due to the charac-teristics of natural regeneration of the species on forest edges and in gaps.It is unclear whether different light intensities in various habitats affect eco-physiological characteristics of saplings and their natural regeneration.In this study,the light intensity in T.sinense habitats was simulated by artificial shading(L1:100%NS(natural sunlight)in the open;L2:50%NS in a forest gap or edge;L3:10%NS in understory)to investigate differences in morphology,leaf structure,physiology,and photosynthesis of 2-year-old sap-lings,and to analyze the mechanism of light intensity on sapling establishment.Significant differences were observed in morphology(including leaf area,and specific leaf area)under different light intensities.Compared to L1 and L3,chloroplast structure in L2 was intact.With increasing time,superoxide dismutase(SOD)and catalase(CAT)activities in L2 became gradually higher than under the other light intensities,while malondialdehyde(MDA)content was opposite.Shading decreased osmoregulation substance contents of leaves but increased chlorophyll.The results suggest that light intensities significantly affect the eco-physiological characteristics of T.sinense saplings and they would respond most favorably at intermediate levels of light by optimizing eco-physiological characteristics.Therefore,50%natural sunlight should be created to promote saplings establishment and population recovery of T.sinense during in situ conservation,including sowing mature seeds in forest edges or gaps and providing appropriate shade protection for seedlings and saplings in the open.

Establishment and characterization of a canine chondrosarcoma cell line:Mango

In the global progress of bone tumor research,established stable and long-lasting transgenic chondrosarcoma(CSA)cell lines are rare,mainly of murine and human origin,while the establishment of canine CSA cell lines has yet to be reported.This study established a canine CSA cell line to facilitate the basic clinical study of canine CSA.Fifty fve cases of canine osteolytic disease were collected,and more than 10 bone tumor samples from dogs with typical clinical signs were used for primary cell culture.A cell line with stable passaging for more than 100 generations and mouse tumorigenic ability was successfully cultured.According to the clinical characteristics of the dog and the histopathological results of the primary tumor,CSA was diagnosed,and the CSA cell line was designated Mango.Immunohistochemical(IHC)results showed that the immunoreactivity of bone gamma-carboxyglutamate protein(BGLAP),secreted protein acidic and rich in cysteine(SPARC),alkaline phosphatase(ALPL),vimentin(VIM)and S100 were positive.However,the immunoreactivity of pan-cytokeratin(PCK),chromogranin A(CGA),and platelet endothelial cell adhesion molecule-1(CD31)was negative.Immunofuorescence(IF)results showed that the protein expressions in the Mango cell line were consistent with the IHC identifcation of the primary tumor.The Mango cell line’s doubling time was 43.92 h,and the cell formation rate exceeded 20%.There were abnormal chromosome numbers,hetero staining with toluidine blue,and certain calcifcation abilities.It could be passaged stably and continuously without changing the cell morphology and characteristics.In vivo,the cells were successfully injected into the nude mice model with a tumorigenic rate of 100%.The immunophenotype of the xenograft tumor was consistent with that of the primary tumor.Therefore,we efectively established a canine CSA cell line.As a promising cell material,this cell line can be used to construct a tumor-bearing model conducive to the subsequent basic research of canine CSA.Moreover,because of its similarity to human CSA,the animal model of CSA is also indispensable for investigating human CSA.

Chemical simulation teaching system based on virtual reality and gesture interaction

Background Most existing chemical experiment teaching systems lack solid immersive experiences,making it difficult to engage students.To address these challenges,we propose a chemical simulation teaching system based on virtual reality and gesture interaction.Methods The parameters of the models were obtained through actual investigation,whereby Blender and 3DS MAX were used to model and import these parameters into a physics engine.By establishing an interface for the physics engine,gesture interaction hardware,and virtual reality(VR)helmet,a highly realistic chemical experiment environment was created.Using code script logic,particle systems,as well as other systems,chemical phenomena were simulated.Furthermore,we created an online teaching platform using streaming media and databases to address the problems of distance teaching.Results The proposed system was evaluated against two mainstream products in the market.In the experiments,the proposed system outperformed the other products in terms of fidelity and practicality.Conclusions The proposed system which offers realistic simulations and practicability,can help improve the high school chemistry experimental education.

Principle for the Installation of Accommodation for Sick guards in Public Health Establishments in Brazzaville in the Republic of Congo

The results of scientific studies of human social facts in the field of health show that the management of a patient should involve the patient’s entourage,whatever the status or size of the health establishment.In healthcare establishments in the Congo,the following are recognised as being responsible for medical care:specialist doctors,doctors,midwives,nurses and care assistants.The patient’s family and close friends are responsible for looking after the patient and financing care.The hospital infrastructure does not provide any space for the patient warden who accompany the patient during reception and hospitalisation.This makes Congolese hospitals inefficient for patient care.How can we integrate the function of the Sick guard and the assistance of the family,in order to reduce the mortality rate and repair the harm caused to patients requiring the presence of relatives during their stay in hospital,which is considered to be a dangerous place?This article examines the functional principles for configuring the space that patient warden would occupy in the patient care system.On the basis of a documentary analysis of sociological and architectural studies of existing facilities,this article proposes a typical accommodation model with the spaces needed to ensure the well-being and effectiveness of the patient warden with the patient.These are rooms with minimum space for 2 to 4 individual beds,equipped with toilets and showers.The accommodation has a dining area,kitchen and laundry facilities.In the future,this accommodation will become part of the hospital estate and may be occupied by orderlies and patient warden recruited by the hospital administration.

HEADLINE

Action plan on establishing standards and metrology system for dual carbon goals released Recently,the National Development and Reform Commission,the State Administration for Market Regulation(SAMR),and the Ministry of Ecology and Environment jointly issued the Action Plan on Further Strengthening the Construction of Standards and Metrology System for Carbon Peak and Neutrality(2024-2025).

2023年标准化工作者职业认知调查问卷分析

Building the capacity of standardization workers can help implement the strategy to enhance China’s strengths via standardization.The National Standardization Development Outline,issued by the Central Government,puts forward strengthening the building of standardization talent teams and establishing a sound vocational ability evaluation and incentive mechanism of standardization talents.

Ethnic Resources Boost Rural Revitalization

Since establishing a business in her rural hometown in 2017,Shi Jia,an ethnic Miao entrepreneur,has been committed to exploring ethnic resources to develop local industries.“Compared with other embroidery styles,the Miao embroidery focuses on vivid expression.At the beginning,it was used to record big events in place of writing,and distinguish tribes by different totems.Its color is brighter,and the design is simpler.There are also many auspicious patterns to pray for good luck,”Shi Jia,a deputy to the National People’s Congress(NPC)and president of a Miao clothing company,told China Today.

Convergence over AI Governance Amid Differences

This year marks a significant year for establishing guardrails around artificial intelligence(AI),with major regions like the EU,the U.S.,and China converging over a risk-based regulatory approach,albeit with distinct differences.This trend reflects a broader trend towards digital sovereignty,with governments seeking increased control over their digital markets and technologies to ensure safety and security,while aiming to boost their AI competitiveness.

Keeping Commitments,Meeting Expectations

How well is FOCAC meeting Africa’s aspirations for development?The idea of establishing the Forum on China-Africa Cooperation(FOCAC)in 2000 initially came from African countries,eager to coordinate and manage their relationship with China.

这一天,值得永恒铭记——写在西藏百万农奴解放纪念日之际

The year 2024 marks the 65th anniversary of democratic reform in Xizang.March 28th of this year is the 15th anniversary of establishing the commemoration day for the emancipation of one million serfs in Xizang.Xizang's democratic reform is the greatest and most profound social transformation in the history of Xizang.Under the leadership of the Communist Party of China(CPC),within just a few decades,these one million former serfs in old Xizang were not only liberated but also transformed into the masters of their nation and society.

上海市医疗卫生机构职工休息室建设需求与对策建议

目的调查分析上海市医疗卫生机构职工休息室建设需求和管理现状,为改善医务职工的工作环境和工作条件提供方法和经验。方法对各级医院和医疗急救中心的医务职工进行线上电子问卷调查,内容包括休息室现状及对休息室需求等,共回收有效问卷1434份。对9家医疗卫生机构开展现场调查,并与休息室建设负责人、管理负责人等15名相关人员进行访谈。结果所调查的医务职工中,13.7%没有时间休息,16.9%在休息室中休息,医疗机构现有职工休息室建设存在设置不均衡、总体数量少、使用率有待进一步提高等问题。医务人员对休息室的需求主要集中于面积、布局、室内环境、功能等方面,其中不同工作特性医务人员的休息需求有所不同。医务职工休息室建设与推行难点主要为用房紧张、法规标准不明确、管理困难、医务职工休息需求不被重视。结论在医务职工休息室规划建设上,建议加大政策支持力度,建立规范化管理制度,并通过加强组织领导、多方协同合作,确保休息室建设改善工作能有效落实。在具体建设方面,应满足医务职工多元需求,可因地制宜规划设计、合理划分功能分区。此外,还应重视宣传总结,营造“健康工作,科学休息”的积极氛围。

The succession of fecal bacterial community and its correlation with the changes of serum immune indicators in lambs from birth to 4 months

Early bacterial colonization and succession within the gastrointestinal tract have been suggested to be crucial in the development of host immunity.In this study,we have investigated the changes in live weight and concentrations of selected serum parameters in relation to their fecal bacterial communities as determined by high throughput sequencing of the 16S r RNA gene over the same period in lambs.The results showed that lambs’growth performance,the serum parameters,fecal bacterial community and fecal bacterial functions were all affected(P<0.05)by age of the lambs.Similarity within age groups of fecal microbiota was lower in the preweaning period and increased sharply(P<0.05)after weaning at 60 days.The similarity between the samples collected from birth to 90 days of age and those collected at 120 days of age,increased(P<0.05)sharply after 30 days of age.Some age-associated changes in microbial genera were correlated with the changes in concentrations of immune indicators,including negative(P<0.05)correlations between the relative abundance of Lachnospiraceae UCG-010,Eubacterium coprostanoligenes group,Ruminococcaceae UCG-005,Ruminococcaceae UCG-009,Ruminococcaceae UCG-013,Ruminiclostridium 6,Ruminococcaceae UCG-008,and Oscillibacter with serum concentrations of lipopolysaccharide(LPS),D-lactate dehydrogenase(DLA),immunoglobulin(IgA,IgM,and IgG),and cytokines(interleukin-1β(IL-1β),IL-6,IL-12,and IL-17),tumor necrosis factor-α(TNF-α),and the relative abundance of these genera increased from 45 days of age.In conclusion,these results suggested that the age-related abundances of particular genera were correlated with serum markers of immunity in lambs,and there might be a critical window in the period from birth to 45 days of age which provide an opportunity for potential manipulation of the fecal microbial ecosystems to enhance immune function.

Learning-Based Dynamic Connectivity Maintenance for UAV-Assisted D2D Multicast Communication

Unmanned aerial vehicles(UAVs) enable flexible networking functions in emergency scenarios.However,due to the movement characteristic of ground users(GUs),it is challenging to capture the interactions among GUs.Thus,we propose a learningbased dynamic connectivity maintenance architecture to reduce the delay for the UAV-assisted device-todevice(D2D) multicast communication.In this paper,each UAV transmits information to a selected GU,and then other GUs receive the information in a multi-hop manner.To minimize the total delay while ensuring that all GUs receive the information,we decouple it into three subproblems according to the time division on the topology:For the cluster-head selection,we adopt the Whale Optimization Algorithm(WOA) to imitate the hunting behavior of whales by abstracting the UAVs and cluster-heads into whales and preys,respectively;For the D2D multi-hop link establishment,we make the best of social relationships between GUs,and propose a node mapping algorithm based on the balanced spanning tree(BST) with reconfiguration to minimize the number of hops;For the dynamic connectivity maintenance,Restricted Q-learning(RQL) is utilized to learn the optimal multicast timeslot.Finally,the simulation results show that our proposed algorithms perfor better than other benchmark algorithms in the dynamic scenario.

First report on establishment and characterization of the extrahepatic cholangiocarcinoma sarcoma cell line CBC2T-2

BACKGROUND Extrahepatic cholangiocarcinoma sarcoma is extremely rare in clinical practice.These cells consist of both epithelial and mesenchymal cells.Patient-derived cell lines that maintain tumor characteristics are valuable tools for studying the molecular mechanisms associated with carcinosarcoma.However,cholangiocarcinoma sarcoma cell lines are not available in cell banks.AIM To establish and characterize a new extrahepatic cholangiocarcinoma sarcoma cell line,namely CBC2T-2.METHODS We conducted a short tandem repeat(STR)test to confirm the identity of the CBC2T-2 cell line.Furthermore,we assessed the migratory and invasive properties of the cells and performed clonogenicity assay to evaluate the ability of individual cells to form colonies.The tumorigenic potential of CBC2T-2 cells was tested in vivo using nonobese diabetic/severe combined immunodeficient(NOD/SCID)mice.The cells were injected subcutaneously and tumor formation was observed.In addition,immunohistochemical analysis was carried out to examine the expression of epithelial marker CK19 and mesenchymal marker vimentin in both CBC2T-2 cells and xenografts.The CBC2T-2 cell line was used to screen the potential therapeutic effects of various clinical agents in patients with cholangiocarcinoma sarcoma.Lastly,whole-exome sequencing was performed to identify genetic alterations and screen for somatic mutations in the CBC2T-2 cell line.RESULTS The STR test showed that there was no cross-contamination and the results were identical to those of the original tissue.The cells showed round or oval-shaped epithelioid cells and mesenchymal cells with spindle-shaped or elongated morphology.The cells exhibited a high proliferation ratio with a doubling time of 47.11 h.This cell line has migratory,invasive,and clonogenic abilities.The chromosomes in the CBC2T-2 cells were polyploidy,with numbers ranging from 69 to 79.The subcutaneous tumorigenic assay confirmed the in vivo tumorigenic ability of CBC2T-2 cells in NOD/SCID mice.CBC2T-2 cells and xenografts were positive for both the epithelial marker,CK19,and the mesenchymal marker,vimentin.These results suggest that CBC2T-2 cells may have both epithelial and mesenchymal characteristics.The cells were also used to screen clinical agents in patients with cholangiocarcinoma sarcoma,and a combination of paclitaxel and gemcitabine was found to be the most effective treatment option.CONCLUSION We established the first human cholangiocarcinoma sarcoma cell line,CBC2T-2,with stable biogenetic traits.This cell line,as a research model,has a high clinical value and would facilitate the understanding of the pathogenesis of cholangiocarcinoma sarcoma.

Mangrove forests as a nature-based solution for coastal flood protection:Biophysical and ecological considerations

Nature-based coastal protection is increasingly recognised as a potentially sustainable and cost-effective solution to reduce coastal flood risk.It uses coastal ecosystems such as mangrove forests to create resilient designs for coastal flood protection.However,to use mangroves effectively as a nature-based measure for flood risk reduction,we must understand the biophysical processes that govern risk reduction capacity through mangrove ecosystem size and structure.In this perspective,we evaluate the current state of knowledge on local physical drivers and ecological processes that determine mangrove functioning as part of a nature-based flood defence.We show that the forest properties that comprise coastal flood protection are well-known,but models cannot yet pinpoint how spatial heterogeneity of the forest structure affects the capacity for wave or surge attenuation.Overall,there is relatively good understanding of the ecological processes that drive forest structure and size,but there is a lack of knowledge on how daily bed-level dynamics link to long-term biogeomorphic forest dynamics,and on the role of combined stressors influencing forest retreat.Integrating simulation models of forest structure under changing physical(e.g.due to sea-level change)and ecological drivers with hydrodynamic attenuation models will allow for better projections of long-term natural coastal protection.

Energy-Efficient Data Collection over Underwater MI-Assisted Acoustic Cooperative MIMO WSNs

Underwater magnetic induction(MI)-assisted acoustic cooperative multiple-input-multipleoutput(MIMO) has been recently proposed as a promising technique for underwater wireless sensor networks(UWSNs).For the more,the energy utilization of energy-constrained sensor nodes is one of the key issues in UWSNs,and it relates to the network lifetime.In this paper,we present an energy-efficient data collection for underwater MI-assisted acoustic cooperative MIMO wireless sensor networks(WSNs),including the formation of cooperative MIMO and relay link establishment.Firstly,the cooperative MIMO is formed by considering its expected transmission range and the energy balance of nodes with it.Particularly,from the perspective of the node’s energy consumption,the expected cooperative MIMO size and the selection of master node(MN) are proposed.Sequentially,to improve the coverage of the networks and prolong the network lifetime,relay links are established by relay selection algorithm that using matching theory.Finally,the simulation results show that the proposed data collection improves its efficiency,reduces the energy consumption of the master node,improves the networks’ coverage,and extends the network lifetime.

Cooperation between China and Iran in addiction medicine: opportunities, challenges and strategies

INTRODUCTION Addiction is a major global risk factor for disability and premature death'and has become a public health and social problem worldwide.Despite substantial investments from governments and research institutionsglobally,current addictionmedicine research remains centred in Western countries.However,theories,models and results from western-centric research may not be applicable to different cultures.Non-Western countries such as China and Iran,and Western countries are committed to establishing addiction prevention and treatment tailored to their unique histories and cultures.To foster a global addiction science,it is important to increase the diversity of evidence without preconceived biases.

宇文氏与巴牙兀惕氏探源

The Yuwen Tribe(宇文)had an important impact on ancient Chinese history,affecting the development of the situation in the Northeast in the early Sixteen Kingdoms period,and establishing the Northern Zhou Dynasty in the late Northern Dynasty,not only unifying northern China,but also laying the foundation for the great unification of the Sui Dynasty.The composition of the Yuwen tribe was relatively complex,which is also a typical example of the blending and development history of northern ethnic groups in ancient China.Therefore,taking the internal clan structure of the Yuwen Tribe as a starting point for research,some new insights will be gained,especially the key to exploring the core clans Sifen(侯汾)and Payetou(破野头)of the Yuwen Tribe.After reviewing and judging the relevant records in Chinese historical materials and multilingual documents,it is believed that the names Sifen(侯汾)and Poyetou(破野头)were respectively the Kehelin(客赫邻)and Bayagut(巴牙惕)in the Mongolian and Yuan Dynasties,namely the Bayagut clan on the grassland;The original meaning of the word Yuwen(宇文)is the tribal leader Lord,which is also the Magalig surname recorded in the Secret History of the Mongols.

Bruce Lee of the Piano World

After winning the 18th International Fryderyk Chopin Piano Competition in Warsaw in 2021 and establishing himself as a rising star,pianist extraordinaire Liu Xiaoyu plans to tour China in October 2023.

《中国智能制造发展研究报告:标准化》发布

Intelligent manufacturing,or smart manufacturing,is the key measure for establishing a modernized industrial system and realizing a new type of industrialization,which can boost China's strength in manufacturing.