建立了一种同柱分离检测44%砜吡草唑·异丙隆悬浮剂中有效成分的高效液相色谱定量方法,确定的色谱条件为250 mm×4.6 mm ODS-C18不锈钢色谱柱和紫外检测器,柱温30℃,甲醇+水溶液(60︰40,体积比)为流动相,检测波长为220 nm。结...建立了一种同柱分离检测44%砜吡草唑·异丙隆悬浮剂中有效成分的高效液相色谱定量方法,确定的色谱条件为250 mm×4.6 mm ODS-C18不锈钢色谱柱和紫外检测器,柱温30℃,甲醇+水溶液(60︰40,体积比)为流动相,检测波长为220 nm。结果表明:在设定的质量浓度范围内,砜吡草唑和异丙隆的线性相关系数r均为0.9999,变异系数分别为1.07%和0.1421%,回收率分别为99.78%和99.50%,说明该方法操作简单、分离效果好,线性关系、准确度和精密度均符合分析的基本要求,可用于制剂产品中砜吡草唑和异丙隆的质量控制。展开更多
For quality control purpose, an approach of fingerprinting and simultaneous quantification of five major bioactive constituents of Rhizoma Coptidis was established via a high-performance liquid chromatograph coupled w...For quality control purpose, an approach of fingerprinting and simultaneous quantification of five major bioactive constituents of Rhizoma Coptidis was established via a high-performance liquid chromatograph coupled with a photodiode array UV detector(HPLC-DAD) and an electrospray ionization mass spectrometer(HPLC-ESI/MS) The compounds were identified on the basis of the comparison of their mass spectra with literature data and those of standard samples and quantified by the HPLC-DAD method. Baseline separation was achieved on an XTerra C18 column(5 μm, 250 mm×4.6 mm i. d.) with linear gradient elution of formate buffer(consisting of 0.5% formic acid, adjusted to pH=4.5 with ammonia) and acetonitrile(consisting of 0.2% formic acid and 0.2% triethylamine). The me- thod was validated for linearity(r^2〉0.9995), repeatability(RSD〈3.1%), intra- and inter-day precision(RSD〈1.8%) with recovery(99.9%-105.1%), limits of detection(0.15-0.35 μg/mL), and limits of quantification(0.53-0.82 μg/mL). The similarities of 32 batches of Rhizoma Coptidis and their classification according to their manufacturers were based on the retention time and peak areas of the characteristic compounds. The five compounds were selected for quality assessment ofRhizoma coptidis via partial least squares analysis(PLS).展开更多
目的建立高效液相一测多评法(high performance liquid chromatography-quantitative analysis of multi-components by single marker,HPLC-QAMS)定量测定甘露消渴胶囊中毛蕊花糖苷、焦地黄苯乙醇苷B_(1)、东莨菪素、东莨菪苷、莫诺苷...目的建立高效液相一测多评法(high performance liquid chromatography-quantitative analysis of multi-components by single marker,HPLC-QAMS)定量测定甘露消渴胶囊中毛蕊花糖苷、焦地黄苯乙醇苷B_(1)、东莨菪素、东莨菪苷、莫诺苷、马钱苷、山茱萸新苷、泽泻醇F、泽泻醇A、24-乙酰泽泻醇A、23-乙酰泽泻醇B的含量,并结合化学计量学对3个厂家的产品质量进行评价。方法采用高效液相色谱仪,流动相选择乙腈-0.2%磷酸,色谱柱为Ultimate XB-C_(18)(250 mm×4.6 mm,5μm)进行梯度洗脱。检测波长分别为330 nm(检测毛蕊花糖苷、焦地黄苯乙醇苷B_(1)、东莨菪素、东莨菪苷)、240 nm(检测莫诺苷、马钱苷、山茱萸新苷)和208 nm(检测泽泻醇F、泽泻醇A、24-乙酰泽泻醇A、23-乙酰泽泻醇B)。以马钱苷为内参物,建立其他10种成分的相对校正因子,并计算各成分含量。采用SPSS 26.0统计软件对甘露消渴胶囊中11种成分含量测定结果进行聚类分析和主成分分析。结果毛蕊花糖苷、焦地黄苯乙醇苷B_(1)、东莨菪素、东莨菪苷、莫诺苷、马钱苷、山茱萸新苷、泽泻醇F、泽泻醇A、24-乙酰泽泻醇A、23-乙酰泽泻醇B分别在7.51~375.50、3.37~168.50、0.46~23.00、1.08~54.00、9.60~480.00、4.77~238.50、1.64~82.00、0.76~38.00、1.95~97.50、0.93~46.50和6.69~334.50μg·mL^(-1)范围内线性关系良好,相关系数(r=0.9991~0.9995),加样回收率及相应的RSD依次为99.11%(1.24%)、98.66%(1.45%)、97.71%(1.60%)、96.98%(0.93%)、100.20%(0.65%)、98.54%(1.18%)、97.90%(1.34%)、96.95%(1.07%)、98.47%(0.94%)、99.10%(0.89%)和100.08%(0.59%)。15批甘露消渴胶囊聚为3类,经主成分分析得4个主成分的累积贡献率达到85.338%。结论所建立的HPLC-QAMS法结合化学计量学可综合评价甘露消渴胶囊的质量。展开更多
Sun Guoxiang, Ding Nan, Song Yuqing, Wang Zhen, Song Liangwei. Determination of Guaiacol salicylate in Guacetisal by HPLCmethod and qualitative identification of relevant substances by HPLC-MS
Medicinal materials Ma Chenchen, Li Bailin, Ou Jie, Wang Jing, Zhao Junhong. Detection of N-acyl-homoserine lactones class signal molecules of quorum sensing secreted by bacteria using high performance liquid chromato...Medicinal materials Ma Chenchen, Li Bailin, Ou Jie, Wang Jing, Zhao Junhong. Detection of N-acyl-homoserine lactones class signal molecules of quorum sensing secreted by bacteria using high performance liquid chromatography-mass spectrometry/ mass spectrometry展开更多
In China, traditional Chinese medicines (TCMs) have been used in clinical applications for thousands of years. The successful hyphenation of high-performance liquid chromatography (HPLC) and mass spectrometry (MS...In China, traditional Chinese medicines (TCMs) have been used in clinical applications for thousands of years. The successful hyphenation of high-performance liquid chromatography (HPLC) and mass spectrometry (MS) has been applied widely in TCMs and biological samples analysis. Undoubtedly, HPLC/MS technique has facilitated the understanding of the treatment mechanism of TCMs. We reviewed more than 350 published papers within the last 5 years on HPLC/MS in the analysis of TCMs. The present review focused on the applications of HPLC/MS in the component analysis, metabolites analysis, and pharmacokinetics of TCMs etc. 50% of the literature is related to the component analysis of TCMs, which show that this field is the most popular type of research. In the metabolites analysis, HPLC coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry has been demonstrated to be the powerful tool for the characterization of structural features and fragmentation behavior patterns. This paper presented a brief overview of the applications of HPLC/MS in the analysis of TCMs. HPLC/MS in the fingerprint analysis is reviewed elsewhere.展开更多
文摘建立了一种同柱分离检测44%砜吡草唑·异丙隆悬浮剂中有效成分的高效液相色谱定量方法,确定的色谱条件为250 mm×4.6 mm ODS-C18不锈钢色谱柱和紫外检测器,柱温30℃,甲醇+水溶液(60︰40,体积比)为流动相,检测波长为220 nm。结果表明:在设定的质量浓度范围内,砜吡草唑和异丙隆的线性相关系数r均为0.9999,变异系数分别为1.07%和0.1421%,回收率分别为99.78%和99.50%,说明该方法操作简单、分离效果好,线性关系、准确度和精密度均符合分析的基本要求,可用于制剂产品中砜吡草唑和异丙隆的质量控制。
基金Supported by the National Natural Science Foundation of China(No.30725045)Shanghai Leading Academic Discipline Project (No.B906)in part by the Scientific Foundation of Shanghai China(Nos.07DZ19728, 06DZ19717 and 06DZ19005)
文摘For quality control purpose, an approach of fingerprinting and simultaneous quantification of five major bioactive constituents of Rhizoma Coptidis was established via a high-performance liquid chromatograph coupled with a photodiode array UV detector(HPLC-DAD) and an electrospray ionization mass spectrometer(HPLC-ESI/MS) The compounds were identified on the basis of the comparison of their mass spectra with literature data and those of standard samples and quantified by the HPLC-DAD method. Baseline separation was achieved on an XTerra C18 column(5 μm, 250 mm×4.6 mm i. d.) with linear gradient elution of formate buffer(consisting of 0.5% formic acid, adjusted to pH=4.5 with ammonia) and acetonitrile(consisting of 0.2% formic acid and 0.2% triethylamine). The me- thod was validated for linearity(r^2〉0.9995), repeatability(RSD〈3.1%), intra- and inter-day precision(RSD〈1.8%) with recovery(99.9%-105.1%), limits of detection(0.15-0.35 μg/mL), and limits of quantification(0.53-0.82 μg/mL). The similarities of 32 batches of Rhizoma Coptidis and their classification according to their manufacturers were based on the retention time and peak areas of the characteristic compounds. The five compounds were selected for quality assessment ofRhizoma coptidis via partial least squares analysis(PLS).
文摘Sun Guoxiang, Ding Nan, Song Yuqing, Wang Zhen, Song Liangwei. Determination of Guaiacol salicylate in Guacetisal by HPLCmethod and qualitative identification of relevant substances by HPLC-MS
文摘Medicinal materials Ma Chenchen, Li Bailin, Ou Jie, Wang Jing, Zhao Junhong. Detection of N-acyl-homoserine lactones class signal molecules of quorum sensing secreted by bacteria using high performance liquid chromatography-mass spectrometry/ mass spectrometry
基金supported by the National Natural Science Foundation of China(No.30873193and No.81073038)
文摘In China, traditional Chinese medicines (TCMs) have been used in clinical applications for thousands of years. The successful hyphenation of high-performance liquid chromatography (HPLC) and mass spectrometry (MS) has been applied widely in TCMs and biological samples analysis. Undoubtedly, HPLC/MS technique has facilitated the understanding of the treatment mechanism of TCMs. We reviewed more than 350 published papers within the last 5 years on HPLC/MS in the analysis of TCMs. The present review focused on the applications of HPLC/MS in the component analysis, metabolites analysis, and pharmacokinetics of TCMs etc. 50% of the literature is related to the component analysis of TCMs, which show that this field is the most popular type of research. In the metabolites analysis, HPLC coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry has been demonstrated to be the powerful tool for the characterization of structural features and fragmentation behavior patterns. This paper presented a brief overview of the applications of HPLC/MS in the analysis of TCMs. HPLC/MS in the fingerprint analysis is reviewed elsewhere.