Objective:To further illuminate a possibme mechanism of Fas/FasL in the treatment of psoriasis, the expression of it and apoptosis of KC were investigated. Methods: With cell culture,immunocytochemistry, the expressio...Objective:To further illuminate a possibme mechanism of Fas/FasL in the treatment of psoriasis, the expression of it and apoptosis of KC were investigated. Methods: With cell culture,immunocytochemistry, the expression of Fas/FasL protein after the treatment with etretin was observed in cultured human normal keratinocytes. Then, the state of apoptosis in cultured keratinocyte after stimulation with etretin was detected with FACS(Fluorescence Activited Cell Sortor). Results:① There was no expression of Fas/FasL protein in the cultured normal human KC. ②After the treatment with etretin, the strongest expression of FasL protein appeared after 16h,so did Fas after 40h.③ The higher the concentration of etretin,the stronger the expression of Fas/FasL protien.Under the same condition,the expression of Fas is stronger than that of FasL.④ Keratinocytes' apoptosis occurred after stimulation with etretin. Conclusion: Fas/FasL system wasn't involved in apoptosis in cultured normol human keratinocytes.But during limited period, the apoptosis of KC could be induced by (etretin),thus it can antagonize benign proliferate of keratinocytes.Our data showed up-regulation of the expression of Fas/FasL and apoptosis in cultured human keratinocytes stimulated by etretin, and its function may be involved in the therapeutic machanism of psoriasis.展开更多
目的探讨银屑灵优化方治疗银屑病的有效性及安全性,以便更好地治疗银屑病,提高银屑病患者的生活质量。方法选择来该院就诊的寻常型银屑病患者76例为研究对象,随机分为观察组和对照组,各38例。给予观察组患者银屑灵优化方口服治疗,给予...目的探讨银屑灵优化方治疗银屑病的有效性及安全性,以便更好地治疗银屑病,提高银屑病患者的生活质量。方法选择来该院就诊的寻常型银屑病患者76例为研究对象,随机分为观察组和对照组,各38例。给予观察组患者银屑灵优化方口服治疗,给予对照组阿维A治疗,共治疗8周。分别于治疗前、治疗4周、治疗8周比较二组皮损程度(Psoriasis Area and Severity Index,PASI)评分;并且分别比较二组患者治疗前后PASI评分情况以及二种治疗方法的有效率及不良反应发生情况。结果治疗前、治疗4周、8周,二组患者的PASI评分未见明显差异,结果无统计学意义(P>0.05);但于治疗4周、8周时二组患者PASI评分均较治疗前减少,差异具有统计学意义(P<0.05)。治疗8周时,观察组患者的有效率与对照组相比,未见明显差异,结果无统计学意义(χ2=0.093,P=0.761);治疗结束时,观察组药物不良反应发生率显著低于对照组,差异具有统计学意义(χ2=4.659,P=0.031)。结论银屑病优化方治疗银屑病效果明显,有效率高,不良反应发生率低,值得临床推广。展开更多
文摘Objective:To further illuminate a possibme mechanism of Fas/FasL in the treatment of psoriasis, the expression of it and apoptosis of KC were investigated. Methods: With cell culture,immunocytochemistry, the expression of Fas/FasL protein after the treatment with etretin was observed in cultured human normal keratinocytes. Then, the state of apoptosis in cultured keratinocyte after stimulation with etretin was detected with FACS(Fluorescence Activited Cell Sortor). Results:① There was no expression of Fas/FasL protein in the cultured normal human KC. ②After the treatment with etretin, the strongest expression of FasL protein appeared after 16h,so did Fas after 40h.③ The higher the concentration of etretin,the stronger the expression of Fas/FasL protien.Under the same condition,the expression of Fas is stronger than that of FasL.④ Keratinocytes' apoptosis occurred after stimulation with etretin. Conclusion: Fas/FasL system wasn't involved in apoptosis in cultured normol human keratinocytes.But during limited period, the apoptosis of KC could be induced by (etretin),thus it can antagonize benign proliferate of keratinocytes.Our data showed up-regulation of the expression of Fas/FasL and apoptosis in cultured human keratinocytes stimulated by etretin, and its function may be involved in the therapeutic machanism of psoriasis.
文摘目的探讨银屑灵优化方治疗银屑病的有效性及安全性,以便更好地治疗银屑病,提高银屑病患者的生活质量。方法选择来该院就诊的寻常型银屑病患者76例为研究对象,随机分为观察组和对照组,各38例。给予观察组患者银屑灵优化方口服治疗,给予对照组阿维A治疗,共治疗8周。分别于治疗前、治疗4周、治疗8周比较二组皮损程度(Psoriasis Area and Severity Index,PASI)评分;并且分别比较二组患者治疗前后PASI评分情况以及二种治疗方法的有效率及不良反应发生情况。结果治疗前、治疗4周、8周,二组患者的PASI评分未见明显差异,结果无统计学意义(P>0.05);但于治疗4周、8周时二组患者PASI评分均较治疗前减少,差异具有统计学意义(P<0.05)。治疗8周时,观察组患者的有效率与对照组相比,未见明显差异,结果无统计学意义(χ2=0.093,P=0.761);治疗结束时,观察组药物不良反应发生率显著低于对照组,差异具有统计学意义(χ2=4.659,P=0.031)。结论银屑病优化方治疗银屑病效果明显,有效率高,不良反应发生率低,值得临床推广。
