Comparative Study on Effects of Arnebia Euchroma (Royle) Johnst Granular and Decoction Forms on Medical AbortionwithMifepristone andMisoprostol

Objective To compare the effects of 2 dosage-forms (granular and decoction) of Arnebia euchroma (royle) Johnst (Arnebia EJ in short below) on medical abortion with that of mifepristone combined with misoprostol Methods Totally 648 women, who had pregnancy of 38-45 d and were willing to terminate pregnancy with mifepristone and misoprostol, were randomly divided into 3 groups, each of which was respectively given granular of Arnebia EJ, placebo granular, or decoction of Arnebia EJ besides mifepristone and misoprostol. The abortion results, bleeding duration, menstruation recovery and side-effects were observed. Results Neither complete abortion rates nor average bleeding durations of the granular group and the decoction group were significantly different (P>0.05). The complete abortion rate and bleeding duration of the two groups were respectively higher and shorter than those of the placebo group (P<0.05). However, the menstruation recov- ery was not significantly different among the three groups (P>0.05). The decoction of Arnebia EJ caused significantly more nausea and vomiting than the other groups (P<0.05). Conclusion The granular form did not have the odor of Arnebia EJ, and caused much less nausea and vomiting compared with the decoction form. The granular and decoc- tion forms were equally effective in improving the results of medical abortion. There- fore it is necessary to conduct further studies on the granular form of Arnebia EJ.

青刺果抗菌肽组分的分离及其稳定性分析

旨在高值化利用青刺果榨油后副产物青刺果粕中的蛋白质,以青刺果粕为原料,提取青刺果蛋白并制备蛋白酶解物,采用活菌吸附法联合反相高效液相色谱(RP-HPLC)从蛋白酶解物中筛选出抗菌肽组分,评价其抑菌活性,并对抑菌活性最好的组分进行稳定性分析。结果表明:筛选出的3个抗菌肽组分(F1、F2和F3)中,F2组分抑菌活性最好,当其质量浓度为10 mg/mL时对金黄色葡萄球菌和大肠杆菌的抑菌活性分别达到45.5%和39.1%;抗菌肽组分F2抑菌活性随温度升高和盐溶液浓度增加均降低,最适pH为6.0,在酸性环境中极不稳定,对酸性蛋白酶、碱性蛋白酶、胃蛋白酶和胰蛋白酶的耐受性均较差,对中性蛋白酶耐受性较好。综上,青刺果粕中的蛋白酶解物具有抑菌活性,具有开发青刺果抗菌肽的潜力。

青刺果抗氧化肽的分离纯化、结构鉴定及其分子对接解析

采用超滤、强阴离子交换层析法分离并纯化青刺果蛋白酶解物,以1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除率为评价指标,筛选具有较好抗氧化活性的肽组分。通过L C-MS/MS鉴定肽序列,结合生物信息学方法分析其理化特性,并进一步利用红外光谱、分子对接技术解析其二级结构特征及与Keap1蛋白的对接位点。研究结果表明,分离得到的4个组分中F-1组分具有更好的抗氧化活性,其DPPH自由基清除率达到27.67%。选择活性更高,且分子质量小于1 kDa的肽组分,测定得到的3条肽(TALDVPPPR、TLSDAGVGGL和DLINGGKDA)的DPPH自由基清除率,其IC 50值分别为0.43、0.83、0.51 mg/mL,其中TALDVPPPR的抗氧化活性相对较高。二级结构分析结果表明,3条抗氧化肽均由α-螺旋、β-折叠和β-转角构成,其中TALDVPPPR的α-螺旋含量相对较低,使其更易与自由基结合。分子对接结果显示,TALDVPPPR、TLSDAGVGGL、DLINGGKDA以氢键和疏水作用与Keap1蛋白紧密结合,释放Nrf2进入细胞核,并激活一系列具有抗氧化和细胞保护作用的蛋白质表达,即激活Keap 1-Nrf2/ARE通路而发挥抗氧化作用。因此,TALDVPPPR、TLSDAGVGGL、DLINGGKDA可作为新型抗氧化剂,研究旨在为青刺果功能性肽的开发利用提供理论参考。

真菌诱导子调控紫草素合成的分子机制探究

为探讨真菌诱导子调控紫草素合成的分子机制,以新疆紫草无菌苗为试材,经尖孢镰刀菌、立枯丝核菌制备成的诱导子生物诱导后,对其根部进行RNA测序和分析。结果表明,与对照组比较,尖孢镰刀菌试验组差异表达基因1735个;立枯丝核菌试验组差异表达基因1043个。GO(gene ontology)分析发现,2个试验组差异表达基因主要富集在细胞过程、细胞组分中膜和分子功能中催化活性等生物学过程。KEGG(kyoto encyclopedia of genes and genomes)分析发现,2个试验组在植物与病原菌互作、植物激素信号转导途径和苯丙素合成等通路均有大量差异表达基因富集。2个试验组差异表达情况相同的转录因子主要包括bHLH、AP2/ERF-ERF和LOB等,并发现参与紫草素合成及其正向调控的AeGHQH、AeDSH1、AeAP、AePAL、AeDI2、AePGT、AeHMGR、AeG10H基因在2个试验组均上调表达,其中尖孢镰刀菌试验组上调表达较显著。以上结果从分子水平探究了新疆紫草对真菌诱导子生物诱导的响应机制,为未来真菌诱导子应用于新疆紫草种植生产奠定了理论基础。

紫草植物染料对天丝织物的印花工艺研究

探究了紫草植物染料在天丝织物上的印花工艺。以海藻酸钠、瓜尔豆胶、羧甲基纤维素钠为原糊,测试印花后织物的K/S值,分析了染料用量、汽蒸温度、汽蒸时间、渗透剂用量对印花效果的影响。结果表明,海藻酸钠作为原糊用于紫草植物染料对天丝织物印花具有良好的印花效果,优化印花工艺参数为:染料7.5%,汽蒸温度105℃,汽蒸时间30 min,渗透剂JFC 3%;在此工艺条件下,印花天丝织物的耐皂洗色牢度和耐摩擦色牢度均能达到3~4级或以上。

Effectiveness of a hydroxynaphthoquinone fraction from Arnebia euchroma in rats with experimental colitis

AIM:To evaluate the potential effectiveness of hydroxynaphthoquinone mixture(HM)in rats with 2,4,6-trinitrobenzene sulfonic acid(TNBS)-induced colitis.METHODS:Colitis was induced by intracolonic administration of TNBS(80 mg/kg,dissolved in 50%ethanol).Rats were treated daily for 7 d with HM(2.5,5,10 mg/kg)and mesalazine 100 mg/kg 24 h after TNBS instillation.Disease progression was monitored daily by observation of clinical signs and body weight change.At the end of the experiment,macroscopic and histopathologic lesions of rats were scored,and myeloperoxidase(MPO)activity was determined.We also determined inflammatory cytokine tumor necrosis factor(TNF)-αlevel by ELISA,Western blotting and immunochemistry to explore the potential mechanisms of HM.RESULTS:After intracolonic instillation of TNBS,animals developed colitis associated with soft stool,diarrhea and marked colonic destruction.Administration of HM significantly attenuated clinical and histopathologic severity of TNBS-induced colitis in a dose-dependent manner.It abrogated body weight loss,diarrhea and inflammation,decreased macroscopic damage score,and improved histological signs,with a significant reduction of inflammatory infiltration,ulcer size and the severity of goblet cell depletion(all P<0.05 vs TNBS alone group).HM could reduce MPO activity.In addition,it also decreased serum TNF-αlevel and down-regulated TNF-αexpression in colonic tissue.This reduction was statistically significant when the dose of HM was 10 mg/kg(P<0.05 vs TNBS alone group),and the effect was comparable to that of mesalazine and showed no apparent adverse effect.The underlying mechanism may be associated with TNF-αinhibition.CONCLUSION:These findings suggest that HM possesses favourable therapeutic action in TNBS-induced colitis,which provides direct pharmacological evidence for its clinical application.

Antibacterial Activity of Crude Extracts of Prinsepia utilis Royle in vitro

The purpose of this study is to isolate the active fraction from the crude extract of Prinsepia utilis Royle and to determine its antibacterial activity against the standard and drug-resistant bacteria strains in vitro. The Prinsepia utilis Royle was extracted in three portions by using petroleum ether, ethanol and water by the continuation recirculate extraction. The antibacterial activity of the extracts were evaluated by the determination of Minimum Inhibitory Concentration （MIC） and Minimum Bactericidal Concentration （MBC） values. The results showed that the ethanol extract and aqueous extract of petroleum ether showed the weakest inhibition. MIC values of the aqueous Prinsepia utilis Royle were found that they had significant effects against bacteria, while ethanol extracts against three standard bacteria （S. aureus ATCC25923, E. coli 44102, Salmonella 50041） were 25, 25, 50 mg·mL^-1 and 50, 100, 100 mg·mL^-1, MIC values of three drug-resistant bacteria （S. aureus GL17, E. coli EYAC08-56, Salmonella STQD2G.） were 50, 50, 50 mg·mL^-1 and 100, 100, 200 mg·mL^-1, respectively. MBC values of two extracts against three standard strains were 25, 50, 100mg·mL^-1 and 50, 100, 200 mg·mL^-1, MBC values of three drug-resistant bacteria were 100, 100, 100mg·mL^-1 and 200, 200, 200 mg·mL^-1, respectively.

Preliminary Isolation and Antibacterial Activity of the Ethyl Acetate Extract of <i>Prinsepia utilis</i>Royle <i>in Vitro</i>

Seven fractions were isolated from the ethyl acetate extract of Prinsepia utilis Royle by silica gel column chromatography. The antibacterial activities determined by disc diffusion method in vitro, indicated that the first and fourth fractions showed better antibacterial activity than the other fractions, while the sixth and seventh fractions did not showed any antibacterial activity. The diameters of the inhibition zone of first and fourth fractions were greater than 10 mm against three standard strains (Staphylococcus aureus ATCC25923, Escherichia coli ATCC44102 and Salmonella sp. CMCC(B)50041) at the concentration of 20 mg/ml. The first fraction was then repeatedly recrystallized in acetone to yield a white snowflake-like compound A, the inhibition zones of which were 14.03 mm, 11.54 mm and 12 mm, respectively. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were measured by broth dilution method at the concentrations ranging from 20 to 0.313 mg/mL. The MIC and MBC values of the first, fourth fractions and compound A were lower than that of oregano oil (positive control) against S. aureus ATCC25923.

THE MECHANISM OF ANTITUMOR ACTIVITY OF TOTAL GLUCOSIDES EXTRACTED FROM CYNANCHUM AURICULA-TUM ROYLE (CA)

Since the chemical structure of total glucosides from Cynanchum Auriculatum Royle (CA) is similar to that of the steroline of Marsdenia Condurago Reich, a compound which exhibits antitumor activity, research into the antitumor activity of CA was carried out. Its mechanism of action was studied in vivo with C 57BL/6 mice bearing Lewis lung carcinoma and in vitro, with two mouse tumor cell lines: S180 and EAC. CA inhibited to a certain extent the growth of subcutaneously inoculated Lewis lung carcinoma and its pulmonary metastasis, and augmented the antitumor effect of cyclophosphamide. It showed a killing effect on the EAC and S180 tumor cells of mice in vitro as well. It blocked the tumor cells of solid Lewis lung carcinoma from entering into the S stage from G1 and inhibited DNA synthesis of S180 and EAC tumor cells of mice in vitro. It also markedly increased the number of mononuclear Mφ of tumor bearing mice, stimulated the macrophagic activity of their intraperitoneal Mφ, raised the percentage of ANAE(+) lymphocytes in peripheral blood and enhanced the ABC reaction and antibody formation in tumor bearing mice.

Enhancement of Shikonin Production in Cell Suspension Cultures of Arnebia Euchroma Employing Two-Liquid-Phase Systems

1 INTRODUCTIONThe production of secondary metabolites using plant cells has been the subject of much inter-est in recent years.Despite that tremendous research efforts had been made in this topic,notmany products have reached the commercial stage.It is generally acknowledged that the mainproblem in this field is the lack of basic knowledge of the biosynthetic routes,and the mechanisms found to bring about the production of such secondary metabolites.There are,however,some techniques that have beneficial effects on the production and ex-

宽谱强脉冲光(BBL)联合盐酸米诺环素和含青刺果油屏障修复霜治疗玫瑰痤疮

目的:评价综合疗法(联合盐酸米诺环素、宽谱强脉冲光和含青刺果油屏障修复霜)治疗玫瑰痤疮的临床有效性和安全性。方法:回顾性分析2021年1月-2021年6月就诊于上海交通大学医学院附属第九人民医院皮肤科门诊,主要表现为红斑、丘疹和毛细血管扩张的玫瑰痤疮患者20例。给予患者口服盐酸米诺环素50 mg,2次/日,连续4周;联合宽谱强脉冲光(Broad band light,BBL)治疗,1次/月,共3次;同时给予患者含青刺果油皮肤屏障修复剂进行日常护肤。治疗3个月后随访1个月,评估治疗有效性和安全性。结果:治疗后,患者治疗有效率为95%,持久性红斑、毛细血管扩张、丘疹和脓疱的治疗评均分较治疗前明显降低(P<0.05)。所有患者治疗期间均未出现严重不良反应。结论:针对以持久性红斑、毛细血管扩张、丘疹和脓疱为主要表现的玫瑰痤疮,盐酸米诺环素、BBL联合含青刺果油屏障修复霜的综合疗法安全有效。

新疆紫草色素在羊毛织物染色中的应用

为系统研究新疆紫草色素在羊毛织物染色中的应用效果,以正交试验优化了紫草色素在羊毛织物上的染色工艺条件。结果表明,采用直接染色工艺,在色素质量分数4%(omf)、温度80℃、pH 4.0及时间60 min的条件下,织物可获得较高的染色深度(K/S值9.2)。将铝盐、铁盐作为媒染剂,用新疆紫草色素对羊毛织物分别进行预媒染、同浴媒染以及后媒染染色,染后织物的各项色牢度比直接染色法均有所提高。

新疆紫草对兔毛织物的染色性能研究

采用新疆产紫草对兔毛织物进行染色,研究色素提取工艺、兔毛织物染色工艺以及环境友好型媒染剂对兔毛织物染色性能的影响。结果表明:在提取温度为50℃、提取时间为2 h、m(紫草)∶m(乙醇)=1∶10、乙醇体积分数为95%、提取次数为2次时,有效色素的提取率可以达到93.87%。采用直接染色工艺时,在染料用量为4%(omf)、pH为4、温度为90℃、时间为60 min的条件下,K/S值较高(9.2)。分别采用铝盐和铁盐作为媒染剂对兔毛织物进行预媒染、同浴媒染以及后媒染,测试染色织物的K/S值以及各项色牢度,结果表明:铝盐预媒染和铁盐后媒染可以达到较好的染色效果。

黔产毛子草化学成分的分离与鉴定

目的:研究黔产毛子草Incarvillea arguta(Royle)Royle乙醇提取物的水部位化学成分。方法:采用硅胶柱层析、大孔树脂柱层析、制备型高效液相等色谱分离方法对毛子草乙醇提取物进行分离纯化,并应用多种波谱技术鉴定化合物结构。结果:从黔产毛子草乙醇提取物的水部位中分离得到19个化合物,分别鉴定为:类叶升麻苷(1)、异类叶升麻苷(2)、地黄苷(3)、米团花苷A(4)、肉苁蓉苷C(5)、连翘酯苷(6)、老鼠簕苷A(7)、车前草苷C(8)、对羟基桂皮酸(9)、咖啡酸(10)、连翘环己醇酮(11)、rengoside B(12)、肌醇(13)、pubinernoid A(14)、cis-2,4-dihydroxy-2-cyclohex-5-en-1-one(15)、8-表去氧马钱子酸(16)、车前醚苷(17)、1-O-β-D-glucopyranosyl-β-D-glucopyranosyl-3,7-dimethyl-2(E)-6-heptdiene(18)、吲哚-3-甲醛(19)。结论:其中,化合物3~8、10~15、18、19为首次从该植物中分离得到。

青刺尖油枯的化感作用及其化感物质的分析

以青稞和豌豆的幼苗为试验材料,评价不同质量浓度青刺尖油枯挥发物对其生长的影响,并研究其化感作用;测定青刺尖油枯气态挥发物对豌豆、青稞幼苗的IC_(50)值;最后,用GC-MS分析测定其挥发性成分.结果表明:在一定条件下,不同质量浓度的青刺尖油枯挥发物对两种植物幼苗的生长均表现出显著的影响,且作用效果随着质量浓度的增加,呈现出一定的规律.对豌豆幼苗的生长,各浓度在1~16 d每日间断处理,都显示抑制效果显著(P≤0.05),随着质量浓度的增加,抑制效果增强;2.88和1.44 g/cm^(3)质量浓度处理的抑制效果与黄花蒿一样,随着质量浓度的增加,抑制效果呈升高的趋势,豌豆幼苗的IC_(50)值为0.752μg/mL.青稞幼苗的生长,各浓度在1~16 d每日间断处理,0.18和0.36 g/cm^(3)质量浓度抑制效果显著(P≤0.05),随着质量浓度的增加,抑制效果减弱;0.72、1.44和2.88 g/cm^(3)质量浓度促进效果显著(P≤0.05),且随质量浓度的增加,促进效果呈先升高再下降的趋势,青稞幼苗的IC_(50)值为4.160μg/mL.结论:青刺尖油枯在0.18~2.88 g/cm^(3)质量浓度对青稞、豌豆的幼苗生长有不同影响,对豆科的豌豆,在此质量浓度范围均表现出抑制效果,而且呈现饱和效应;对禾本科的青稞,低质量浓度表现为抑制,高质量浓度表现为促进.GC-MS分析测定表明,青刺尖油枯有40种挥发性物质,主要有萜类、醇类、酯类、烷烃类和氮类等5大类,其中,萜类含量最高,是挥发性物质的主要成分.

新疆软紫草提取物对HepG2细胞凋亡的影响及其抗小鼠原位肝癌的作用

目的研究新疆软紫草根[Arnebia euchroma(Royle)Johnst,AE]不同提取物诱导人肝癌细胞株(HepG2)的凋亡作用,并通过小鼠原位肝癌模型验证其抗肝癌作用,初步探究AE提取物的抗肝癌作用。方法首先采用MTT法和Annexin V-FITC/PI双染色法检测AE各提取物对HepG2细胞的抗增殖以及促凋亡作用,采用Western blot法检测了细胞中Bcl-2凋亡家族蛋白的表达水平,综合上述实验结果,筛选出促凋亡作用明显的提取物进行抗小鼠原位肝癌的实验,评价了给药前后原位肝癌小鼠的脏器指数、肝功能指标及组织切片。结果随着AE提取物极性的降低,对HepG2细胞抗增殖、促凋亡作用增强,AE石油醚部位(AEP)抗增殖和诱导凋亡的作用最明显;在AEP剂量为1.56μg·L^(-1)时,Bcl-2蛋白水平明显下降(P<0.05),且呈剂量依赖性,Bad蛋白表达水平在AEP剂量为3.125μg·L^(-1)时明显上调(P<0.05)。动物实验验证了AEP可以改善肝功能,减缓肝癌发展进程的作用。结论研究表明AEP对肝细胞癌的发展具有一定的抑制作用,发挥该作用与调节Bcl-2家族蛋白有关。

滨海白首乌酸乳的制备及其抗氧化能力

该文以滨海白首乌粉和全脂乳粉为主要原料,通过单因素试验研究白首乌粉添加量、发酵剂接种量、蔗糖添加量及发酵温度对滨海白首乌酸乳的品质和总抗氧化能力的影响,并利用正交试验对其制备条件进行优化,同时研究其贮藏特性。结果表明,全脂乳粉中添加2.0%的滨海白首乌粉和5.0%的蔗糖,接种0.015 U/kg Y450A发酵剂,40.0℃发酵5 h,制备的滨海白首乌酸乳总抗氧化能力为0.1292 mmol/L,显著高于未发酵和不含滨海白首乌粉的酸乳(P<0.05),且酸甜适中,风味和口感良好。异丙醇、2,3-丁二酮、正己醇、2-庚酮、仲丁基亚硝酸酯及三乙二醇单乙醚为其主要的风味物质。4℃贮藏9 d后,滨海白首乌酸乳的总抗氧化能力为0.1035 mmol/L,乳酸菌活菌数为1.57×10^(8)CFU/mL,并保持了良好的感官品质。

青刺果油组合物的抗氧化及保湿活性研究

寻找青刺果油组合物来提高产品抗氧化能力及保湿活性。将青刺果油组合物应用于产品中,通过DPPH自由基清除率以及失水率的测定,研究其抗氧化能力及保湿活性。结果表明,在干燥环境下青刺果油组合物样品随时间增加,保湿率逐渐降低,但每个时间段内其保湿率均高于对照样品(青刺果油含量0.11%);与对照样品相比,添加了虾青素、橙皮素的青刺果油样品对DPPH自由基清除能力较强;将虾青素和橙皮素组合后,提取组合物浓度在50%时DPPH自由基清除能力也可达到90%左右。研究初步验证了青刺果油组合物具有较强的自由基清除能力和保湿性能,为拓展青刺资源的综合利用供了初步的应用研究。

秦岭特色药用植物桃儿七内生真菌代谢产物的分离鉴定及生物活性研究

以秦岭无病害健康桃儿七内生烟色拟盘多毛孢菌为研究对象,采用萃取法从菌丝中分离活性代谢产物,并对其结构进行鉴定,采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)比色法检测了代谢产物的细胞毒活性,采用Ellman光度法检测了代谢产物的乙酰胆碱酯酶抑制活性,并检测了代谢产物的α-葡萄糖苷酶抑制活性。结果表明,在桃儿七内生烟色拟盘多毛孢菌代谢产物中分离并鉴定了10个萜类化合物,其中化合物1-1、1-2、1-3和1-4为新化合物。化合物1-3、1-4和1-8对A549、HeLa和HepG2细胞均表现出明显的细胞毒活性;化合物1-3对乙酰胆碱酯酶表现出较强的抑制活性;化合物1-1、1-2、1-3和1-4对α-葡萄糖苷酶均未表现出明显的抑制活性。表明,基于桃儿七内生烟色拟盘多毛孢菌分离出具有抗肿瘤活性和乙酰胆碱酯酶抑制活性的萜类化合物,为桃儿七内生真菌用于抗肿瘤及治疗阿尔兹海默病新药开发提供了新思路。

西藏阿里地区主要造林树种扦插育苗及营造林技术研究

西藏阿里地区植被稀少、自然环境极其恶劣,为加速乡土树种育苗扩繁,提高造林成活率和保存率,采用不同年份班公柳和秀丽水柏枝插条设置无地膜、有地膜、ABT1号生根粉5个浓度梯度(CK、50、100、150、200 mg/L)处理进行扦插试验,选取沙柳、班公柳、秀丽水柏枝设置插杆长度30、40、50 cm+露出地面长度0、5、10 cm插杆造林试验,选取蒙古柳、沙柳设置平茬(1/2)、平茬(1/3)、未打头(CK)平茬复壮营造林试验。结果表明:(1)覆膜较无地膜能提高30%扦插成活率,二年生班公柳和秀丽水柏枝插条生长指标最好,ABT1号生根粉浓度为100 mg/L时,班公柳(84.6%)和秀丽水柏枝(100%)成活率最高。(2)沙柳(96%)和班公柳(80%)的插杆造林成活率较高,秀丽水柏枝成活率最低,3种柳树在高度、地径、丛生数上体现出不同优势。(3)不同程度的柳树平茬营造林成活率可达62.5%以上,平茬(1/2)成活率最高,在平茬复壮后分枝数翻倍增长。在阿里地区扦插育苗扩繁是可行的,不同插杆方式、平茬复壮均可提高营造林成效。