A novel Eucommia antifungal peptide,named EAFP3,was isolated from the bark of Eucommia ul-too/des by NaC1 extract,gel filtration and reverse phase high performanee liquid ehromatography.Themolecular mass of EAFP3 is 4...A novel Eucommia antifungal peptide,named EAFP3,was isolated from the bark of Eucommia ul-too/des by NaC1 extract,gel filtration and reverse phase high performanee liquid ehromatography.Themolecular mass of EAFP3 is 4157.3 Da,and its partial amino acid sequenee is-.LYQQLIAGITLNK.-.EAFP3 exerts an inhibitory activity against Candida albicans in vitro and the drug concentration requiredfor 50% growth inhibition(IC50)is 31.25μg/mL.展开更多
目的:为了评价杜仲再生皮质量,测定了杜仲三次再生皮中降压有效成分松脂醇二葡萄糖苷(P inores-inol d iglucoside,PDG)含量。方法:采用HPLC法,色谱柱为国产YWG C18柱(46×250 mm,10μm),以甲醇-水(30∶75)为流动相,流速1.0 m l/m ...目的:为了评价杜仲再生皮质量,测定了杜仲三次再生皮中降压有效成分松脂醇二葡萄糖苷(P inores-inol d iglucoside,PDG)含量。方法:采用HPLC法,色谱柱为国产YWG C18柱(46×250 mm,10μm),以甲醇-水(30∶75)为流动相,流速1.0 m l/m in,柱温为室温,检测波长为277 nm,进样量为10μl。结果:第一次剥皮后的再生皮中PDG含量略高于原生皮;第二次剥皮后的再生皮中PDG含量与剥皮前的一次再生皮相当;第三次剥皮后的再生皮中PDG含量明显低于剥皮前的二次再生皮。结论:为了保证药材质量,杜仲剥皮再生可进行两次,每次间隔时间不少于5年,第三次剥皮后的再生皮中PDG含量有待提高。展开更多
基金the National Natural Science Foundation of China(30660146)the Ministry of Sciences of Technology Program of China(2003CCA02600)
文摘A novel Eucommia antifungal peptide,named EAFP3,was isolated from the bark of Eucommia ul-too/des by NaC1 extract,gel filtration and reverse phase high performanee liquid ehromatography.Themolecular mass of EAFP3 is 4157.3 Da,and its partial amino acid sequenee is-.LYQQLIAGITLNK.-.EAFP3 exerts an inhibitory activity against Candida albicans in vitro and the drug concentration requiredfor 50% growth inhibition(IC50)is 31.25μg/mL.
文摘目的:为了评价杜仲再生皮质量,测定了杜仲三次再生皮中降压有效成分松脂醇二葡萄糖苷(P inores-inol d iglucoside,PDG)含量。方法:采用HPLC法,色谱柱为国产YWG C18柱(46×250 mm,10μm),以甲醇-水(30∶75)为流动相,流速1.0 m l/m in,柱温为室温,检测波长为277 nm,进样量为10μl。结果:第一次剥皮后的再生皮中PDG含量略高于原生皮;第二次剥皮后的再生皮中PDG含量与剥皮前的一次再生皮相当;第三次剥皮后的再生皮中PDG含量明显低于剥皮前的二次再生皮。结论:为了保证药材质量,杜仲剥皮再生可进行两次,每次间隔时间不少于5年,第三次剥皮后的再生皮中PDG含量有待提高。