正交试验优选十枣汤的提取工艺

目的优选十枣汤的提取工艺。方法以十枣汤中大戟二烯醇的含量及干膏得率的综合评分为评价指标,在单因素试验基础上,采用L9(34)正交试验考察加水量、提取时间和提取次数对十枣汤提取工艺的影响。结果最佳提取工艺为提取2次,提取时间为1.5 h,加入10倍量水。验证试验结果显示,5份样品中大戟二烯醇的平均含量为0.99 mg/g,RSD为1.24%(n=5);平均干膏得率为44.01%,RSD为1.64%(n=5)。结论优选的提取工艺合理、可行。

HPLC测定蒙药哈布德仁-9中大戟二烯醇的含量

目的:建立蒙药哈布德仁-9中大戟二烯醇的含量测定方法。方法:采用HPLC测定制剂中大戟二烯醇的含量。色谱条件为:Acclaim^(TM)120 C_(8)色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-水(93:7);流速为1.0 mL·min^(-1);柱温为22.6℃;检测波长为210 nm。结果:大戟二烯醇对照品在0.0875~3.50μg范围内时,进样量与峰面积呈良好的线性关系,r=0.9999;平均回收率为98.19%,RSD为0.48%(n=6)。结论:建立的HPLC法操作简便、快速、灵敏度高、专属性好,为蒙药哈布德仁-9质量控制提供依据。

Investigation of the processing technology and mechanism of Kansui radix by licorice based on response surface methodology and integrative pharmacology

Background:The herbs should be processed by different methods before use,and the efficacy and toxicity of Chinese herbal medicines may change,which may enhance efficacy and reduce toxicity after processing.Gansui(Kansui radix)is a common clinical herbal medicine,and there are considerable changes in its toxicity and efficacy after processing.Gaocao(Glycyrrhizae radix et rhizome)has a detoxifying effect.Methods:Using the contents of euphorbiadienol and the alcohol-soluble extract of Glycyrrhizae radix-processed Kansui radix as evaluation indexes,response surface methodology was used to optimize the processing technology of Kansui radix by exploring the effects of Glycyrrhizae radix et rhizoma-addition amount,frying temperature,and frying time on the processing technology of Kansui radix.Meanwhile,response surface software was used to analyze experimental data to determine the processing parameters of Kansui radix by Glycyrrhizae radix et rhizoma.Integrative Pharmacology-based Research Platform of Traditional Chinese Medicine is used to analysis the potential ingredients of Kansui radix and verify the potential ingredients by western blotting.Results:The technology of Glycyrrhizae radix et rhizoma-processed Kansui radix was optimized for the Glycyrrhizae radix et rhizoma-addition amount of 27%,frying temperature of 180℃,and frying time of 11 min.The network pharmacology analysis revealed that Kansui radix could cause kidney,liver,and heart injury by the PI3K/AKT signaling pathway.Kansui radix at high or low dose could decrease the ratio of p-AKT/AKT while Glycyrrhizae radix et rhizoma-processed Kansui could increase it.Conclusion:The model established by response surface methodology is relatively accurate and can predict the contents of euphorbiadienol and alcohol extract of processed Kansui radix.The toxic effects and its mechanism of action of Kansui radix and processed Kansui radix on kidney,liver,and heart,from the perspective of systems biology,have provided scientific evidence to its clinical application.

醋制前后京大戟饮片石油醚部位的HPLC指纹图谱比较

目的:建立京大戟饮片石油醚部位的指纹图谱,比较京大戟醋制前后石油醚部位化学成分的变化。方法:以大戟二烯醇为对照品,采用高效液相色谱法。色谱柱为汉邦Benetnach C8(250 mm×4.6 mm,5μm),流动相为0.3%磷酸溶液-乙腈(梯度洗脱),检测波长为203 nm。结果:10批京大戟和醋京大戟分别有11个共有色谱峰;京大戟和醋京大戟有8个共有峰。相似度均>0.90。结论:醋制对京大戟化学成分有一定的影响。该方法稳定、准确、可靠,能明显区分京大戟和醋京大戟饮片的内在质量,可为京大戟饮片的质量控制提供依据。