New reagents for immunofluorescence analysis of carbazole series containing fluorinated β-dicarbonyl fragments and carboxylic substituent groups separated by spacers of different lengths from the light-gathering carb...New reagents for immunofluorescence analysis of carbazole series containing fluorinated β-dicarbonyl fragments and carboxylic substituent groups separated by spacers of different lengths from the light-gathering carbazole scaffold have been developed. The markers in complex with Eu<sup>3+</sup> ions possess stability in the aqueous phase, intense and prolonged luminescence (τ 550 - 570 μs) with characteristic emission maxima in the region of 615 nm and excitation wavelengths in the region of 380 - 390 nm, which distinguishes them from most of the analogs used. In the study of marker conjugation with streptavidin, a reagent containing 4 - 5 europium labeling complexes based on spacer-containing carbazole tetraketone was obtained. The marker-doped silicate nanoparticles exhibit intense and long-lived luminescence in the characteristic region.展开更多
A novel chelate Eu(TTA) 2(PMTBBP)Phen, which contained ligand 1 pheny 3 methyl 4 (4 t butylbenzoyl) 5 pyrazolone(HPMTBBP), 4,4,4 trifluro 1 (2 thienyl) 1,3 butandione(HTTA) and phenanthroline(Phen), was synthesized....A novel chelate Eu(TTA) 2(PMTBBP)Phen, which contained ligand 1 pheny 3 methyl 4 (4 t butylbenzoyl) 5 pyrazolone(HPMTBBP), 4,4,4 trifluro 1 (2 thienyl) 1,3 butandione(HTTA) and phenanthroline(Phen), was synthesized. Its chemical structure was elucidated by IR, UV, 1H NMR, MS, DSC and elementary analysis. The influence of acylpyrazolone on fluorescent intensity of the new chelate was studied. The results showed that Eu(TTA) 2(PMTBBP)Phen had more excellent PL properties and better film formation than that of Eu(TTA) 3Phen. [WT5HZ]展开更多
文摘New reagents for immunofluorescence analysis of carbazole series containing fluorinated β-dicarbonyl fragments and carboxylic substituent groups separated by spacers of different lengths from the light-gathering carbazole scaffold have been developed. The markers in complex with Eu<sup>3+</sup> ions possess stability in the aqueous phase, intense and prolonged luminescence (τ 550 - 570 μs) with characteristic emission maxima in the region of 615 nm and excitation wavelengths in the region of 380 - 390 nm, which distinguishes them from most of the analogs used. In the study of marker conjugation with streptavidin, a reagent containing 4 - 5 europium labeling complexes based on spacer-containing carbazole tetraketone was obtained. The marker-doped silicate nanoparticles exhibit intense and long-lived luminescence in the characteristic region.
文摘A novel chelate Eu(TTA) 2(PMTBBP)Phen, which contained ligand 1 pheny 3 methyl 4 (4 t butylbenzoyl) 5 pyrazolone(HPMTBBP), 4,4,4 trifluro 1 (2 thienyl) 1,3 butandione(HTTA) and phenanthroline(Phen), was synthesized. Its chemical structure was elucidated by IR, UV, 1H NMR, MS, DSC and elementary analysis. The influence of acylpyrazolone on fluorescent intensity of the new chelate was studied. The results showed that Eu(TTA) 2(PMTBBP)Phen had more excellent PL properties and better film formation than that of Eu(TTA) 3Phen. [WT5HZ]
