基于感性工学和改进EW-AHP的露营茶具创新设计

目的构建露营茶具的创新设计开发机制,以满足人的感性需求,进而提升对露营茶具的使用情感。方法利用网络爬虫搜集整理用户感性词汇及样本图片,分析用户在露营过程中对露营茶具产品的体验感受,建立了露营茶具感性词汇评价体系,并使用改进EW-AHP计算出各指标的权重,明确感性词汇与露营茶具之间的关联性。结果得出三个产品创新设计方向,最终通过模糊综合分析法对感性词汇评分,验证了设计方案的可行性。结论通过融合感性工学和改进EW-AHP理论,使搜集、整理、分析用户需求的过程更客观准确,为露营茶具的设计与优化提供了数据依据,并对设计结果进行综合评估,设计出满足用户体验需求的现代露营茶具产品。

EWSR1-FLI1通过代谢重编程影响尤文肉瘤发生发展的研究进展

尤文肉瘤是发生于儿童和青少年的第二常见恶性原发性骨肿瘤,该病恶性程度极高,患者病程短,转移事件发生快,严重危害儿童和青少年的健康。肿瘤细胞通过一系列复杂的调控机制促进包括糖、氨基酸、脂类等在内的营养物质发生代谢重编程,进而维持肿瘤高代谢状态,促进肿瘤发生、发展;同时代谢重编程相关产物可作用于肿瘤微环境中各细胞组分,抑制肿瘤免疫反应,甚至诱导耐药事件的发生。尤文肉瘤断裂区域1(EWSR1)-佛氏白血病病毒整合蛋白1(FLI1)融合蛋白是尤文肉瘤的关键促癌分子,具有多重功能和复杂的调控机制,了解EWSR1-FLI1对尤文肉瘤细胞代谢变化的影响及其相关调控机制,可为探究尤文肉瘤的病因、寻找有效的诊疗靶点及治疗策略提供线索。本文就EWSR1-FLI1影响尤文肉瘤代谢重编程的相关研究进展进行汇总与剖析,旨在为该病的早期诊断和精准治疗提供新的思路。

Unlocking epigenetics for precision treatment of Ewing’s sarcoma

Ewing’s sarcoma(EWS)is a highly aggressive malignant bone tumor primarily affecting adolescents and young adults.Despite the efficacy of chemoradiotherapy in some cases,the cure rate for patients with metastatic and recurrent disease remains low.Therefore,there is an urgent need for innovative therapeutic approaches to address the challenges associated with EWS treatment.Epigenetic regulation,a crucial factor in physiological processes,plays a significant role in controlling cell proliferation,maintaining gene integrity,and regulating transcription.Recent studies highlight the importance of abnormal epigenetic regulation in the initiation and progression of EWS.A comprehensive understanding of the intricate interactions between EWS and aberrant epigenetic regulation is essential for advancing clinical drug development.This review aims to provide a comprehensive overview of both epigenetic targets implicated in EWS,integrating various therapeutic modalities to offer innovative perspectives for the clinical diagnosis and treatment of EWS.

Primary Ewing sarcoma of the kidney mimicking cystic papillary renal cell carcinoma in an older patient:A case repor

BACKGROUND Ewing’s sarcoma(ES)is a neuroectodermal tumor that typically occurs in the bones and soft tissues of children and young adults.Primary renal ES is rare;only a few cases and a small case series have been documented,and only four cases involved primary renal ES in older people(>65 years old).CASE SUMMARY Herein,we describe the radiological and pathological features of primary renal ES in an older person.A 76-year-old man complained of poor oral intake and was found to have a large cystic renal mass with indistinct margins on computed tomography.Ultrasound-guided biopsy revealed that the tumor contained small round blue cells.The patient underwent a right radical nephrectomy.The tumor cells showed diffuse membranous CD99,and nuclear friend leukemia integration 1 transcription factor and NK2 Homeobox 2.Fluorescence in situ hybridization revealed EWSR1 translocation.Postoperatively,18F-fluorodeoxyglucose positron emission tomography revealed no evidence of metastasis.The patient was diagnosed with primary renal ES.Six months following the surgery,local recurrence and distant metastasis were observed.Primary renal ES is rare and often lethal in older individuals.The specific imaging findings are unknown,and treatment protocols have not been standardized.CONCLUSION This case report describes the radiological and pathological features of primary renal ES in an older person.

基于分级分类和改进AHP-EW-TOPSIS的电力系统充裕性评估方法

电力系统充裕性评估对电力系统的规划建设具有重要的意义,传统的单概率性指标评估方法已经不能满足随新能源渗透率不断提升、场景复杂化、短缺事件多样化趋势下电力系统充裕性评估的需求。首先,对短缺事件以最大短缺功率等效化处理,基于改进最小二乘法进行充裕性事件分区特征点拟合,构建短缺事件集分级分类的充裕性多指标体系。然后,基于蒙特卡洛法生成多组随机短缺样本数据集,结合改进AHP-EW-TOPSIS法确定各分区指标的全局权重,并得到综合评分降落曲线。最后,根据评分降落曲线构建综合评价结果判据区间。算例以某市实际运行数据为基础进行仿真验证,结果表明,所提方法可结合主观要求和不同尺度充裕性事件的客观特性,得到更加全面、透明的充裕性评估结果。

一测多评法测定法制半夏曲中11种成分含量及其GRA、EW-TOPSIS质量评价

目的采用一测多评(QAMS)法同时测定法制半夏曲中肌苷、鸟苷、腺苷等11种成分含量,并建立其灰色关联度分析(GRA)联合熵权逼近理想解排序分析法(EW-TOPSIS)综合质量评价方法。方法采用Shimadzu C 18色谱柱;乙腈-0.5%醋酸为流动相,梯度洗脱,流速1.0 mL·min-1;检测波长254和290 nm。以对甲氧基肉桂酸乙酯为内参比物质,计算其他10个成分的相对校正因子(RCF),测定各成分含量。采用GRA联合EW-TOPSIS模型对法制半夏曲进行综合质量评价。结果法制半夏曲中11种成分在一定浓度范围内线性关系良好,相关系数均>0.999;平均加样回收率96.94%~100.12%(RSD<2.0%,n=9);QAMS与外标法(ESM)实测值无明显差异。GRA模型相对关联度0.2903~0.6187,EW-TOPSIS模型相对接近度0.2114~0.6343;GRA和EW-TOPSIS模型综合评价结果基本一致。结论QAMS法便捷、准确,可用于法制半夏曲多指标成分定量控制,GRA联合EW-TOPSIS模型可用于法制半夏曲综合质量评价。

Bioinformatics Analysis of the Biological Properties of Ewing Sarcoma

Purpose: Bioinformatics-based approach to screen and analyze differentially expressed genes associated with the biological characteristics of Ewing sarcoma. Means: The GSE17674 dataset was selected for analysis, obtained by data retrieval based on the GEO public database. The R language limma toolkit was used to screen DEmRNAs. After the data were normalized, the Metascape online analysis software and the R language clusterProfiler package were used to analyze the GO function and KEGG pathway enrichment of DEmRNAs lines, respectively. The string database was selected for PPI analysis, and the results were imported into Cytoscape software to derive the core modules and predicted core genes. The genes selected above were analyzed for tissue localization specificity. Results: Through the analysis of GSE17674, differentially expressed genes were screened out, and GO and KEGG analyses were performed on the differentially expressed genes. The GO functional enrichment analysis was mainly enriched in the process of muscle system, muscle contraction, myocyte development, contractile fibers, myogenic fibers, myofibers, myofibrillar segments, actin binding, structural composition of muscle, and actin filament binding. KEGG pathway analysis showed that the core pathways associated with the development of ES were the core genes for myocardial contraction, congestive cardiomyopathy, and hypertrophic cardiomyopathy. Five Hub genes were obtained based on Cytoscape prediction. Tissue localization specificity analysis of Hub genes was performed, and a total of 2 Hub genes with tissue specificity were screened;MYH6 was specifically expressed in cardiac cells and MYL1 was specifically expressed in skeletal muscle cells. Conclusions: The differential genes screened will help to understand the molecular mechanisms underlying the highly invasive and metastasis-prone biological characteristics of ES, as well as provide new ideas for clinical drug-targeted treatment of ES.

基于EW-AHP改良未确知测度理论的泥石流危险性评价方法

针对泥石流危险性评价中确定指标权重过度主观的缺陷,以甘肃省陇南市武都区两水村大湾沟泥石流为例,根据其地质环境特征,选取一次泥石流最大冲出量、流域面积、主沟长度、流域相对高差、泥沙补给段长度比、24 h最大降雨量、人口密度共7个因子作为泥石流危险性评价指标,引入熵权和层次分析法(EW-AHP)组合赋权确定指标权重,建立基于未确知测度理论的单钩泥石流危险性评价模型。对研究区泥石流危险性评价结果表明:结合评价指标的主、客观赋权,本评价模型的评价结果较符合现场调查结论,说明评价方法合理有效,可为今后泥石流危险性评价提供一种新途径。

EW75镁合金镧掺杂硫酸盐稀土转化膜的防护性能研究

通过析氢测试、扫描电子显微镜、全浸实验和电化学测试等手段探究优化稀土转化液配方对稀土转化膜性能的提升。配方中加入硫酸根离子可以有效地改善稀土转化膜无法均匀成膜且与基体结合力不好的现象,改善了膜层成膜均匀性和结合力。在析氢实验中,改进的膜层耐蚀性能有了显著提升,浸泡14天腐蚀速率依旧恒定,说明膜层具有长期稳定的保护作用。全浸实验中经过阶段性腐蚀,膜层仍然完整均匀,无脱落现象出现。动电位极化测试中,改进膜层自腐蚀电位正移,腐蚀电流约为2.152×10^(−5)A/cm^(2),阻抗测试中改进膜层也呈现更好的耐腐蚀性能。

基于EW-FAHP-GIS耦合的煤层顶板富水性评价

以薛虎沟煤矿10号煤层为研究对象,在分析煤层顶板富水性影响因素的基础上,运用熵权(EW)与模糊层次分析(FAHP)综合赋权法建立了顶板含水层富水性的脆弱性指数模型,基于地理信息系统(GIS)对煤层顶板含水层富水性进行评价。研究表明,10号煤层顶板含水层富水性的主控因素为含水层厚度、岩层脆塑性比、钻孔取芯率、钻孔漏失量和岩石质量指标,并建立了相应的脆弱性指数模型,据此进行K2含水层富水性评价,划分了富水性分区,为煤矿制定和实施合理的防治水规划提供科学依据。

基于多组分定量联合PCA、OPLS-DA及EW-TOPSIS分析的癣湿药水综合质量评价

目的:建立癣湿药水多组分定量检测方法,通过主成分分析法(PCA)、正交偏最小二乘法-判别分析(OPLS-DA)联合熵权优劣解距离法(EW-TOPSIS)对多组分含量测定结果进行差异性分析,评价其综合质量。方法:采用高效液相色谱法,Global Chromatography C_(18)色谱柱,以乙腈-0.1%磷酸水溶液为流动相,同时测定12批癣湿药水中柠檬苦素、吴茱萸碱、吴茱萸次碱、欧前胡素、蛇床子素、异欧前胡素、升麻素、5-O-甲基维斯阿米醇苷、土荆皮丙酸、土荆皮乙酸、羟基-α-山椒素、羟基-β-山椒素、羟基-γ-山椒素含量,通过化学计量学和EW-TOPSIS对定量结果进行分析。结果:13个成分在各自范围内线性关系良好(r>0.999),平均加样回收率为96.90%~100.09%(n=9,RSD<2.0%)。PCA结果显示12批癣湿药水聚为3类;OPLS-DA结果表明蛇床子素、土荆皮乙酸、羟基-α-山椒素、升麻素、柠檬苦素和欧前胡素为癣湿药水差异特征标志物;EW-TOPSIS结果显示欧氏贴近度为0.1887~0.6920,不同企业间产品13个成分含量存在一定差异。结论:所建立方法操作便捷、结果准确,可用于癣湿药水质量的综合评价,多组分定量联合PCA、OPLS-DA及EW-TOPSIS亦可用于其他中药制剂检测及质量评价中。

2023年土耳其7.8级强震前烟台台伸缩仪EW分量固体潮畸变

烟台台SS-Y伸缩仪EW分量在2023-02-06土耳其7.8级双震前2 d开始出现急速上升趋势变化,至6日凌晨开始下降,变化幅度达到9.58×10^(-8),震后维持快速上升趋势。对烟台台伸缩仪稳定性指标进行判定,认为仪器稳定;经现场核实,未发现环境干扰。最后对烟台台伸缩仪固体潮畸变特征进行分析,并基于畸变与远震前兆的关系进行初步探讨。

EwE模型在水生态系统中的应用研究及长潭水库案例分析

EwE(Ecopath with Ecosim)模型是一种用于定量研究水生态系统食物网结构和能量流动特征的模型。总结了EwE模型中Ecopath、Ecosim、Ecospace和Ecotracer模块的原理,综述了该模型在不同类型水生态系统中的应用研究进展,发现Ecopath可用来评估海洋和淡水生态系统的成熟度并确定关键种和生态容量,明确浮游植物生产量(P)对生态系统总通量(TST)的重要贡献;Ecosim用于在时间尺度上揭示关键种捕捞、港口建设等人类活动对生态系统结构和功能的影响机制;Ecospace可用来阐明海上平台建设、发电厂运行、火山爆发等外部因素影响下渔业经济和生态系统结构的空间差异性;Ecostracer可用来追踪同位素、重金属和新型污染物等物质在食物网中的迁移过程。采用Ecopath分析了长潭水库的营养结构和能量流动,结果表明,长潭水库营养级介于1.000~3.093,食物网结构简单,TPP/TR为2.445,是一个相对成熟的生态系统,能量传递效率较低。未来应加强气候变化和人类活动对水生态系统结构演替的影响研究,推进新污染物在食物网中富集特征研究,为水域生态系统健康状态评估和渔业经济发展政策的调整提供支撑。

多房棘球蚴RNA结合蛋白EWS的原核表达及其细胞定位与功能的初步鉴定

通过对多房棘球绦虫(Echinococcus multilocularis)RNA结合蛋白EWS(EmEWS)基因的原核表达、抗体制备、细胞定位等研究,初步探究EmEWS基因特征以及与RNA的结合能力。根据Wormbase数据库中EmEWS基因序列设计引物,利用RT-PCR扩增EmEWS基因并构建重组表达质粒,诱导表达、纯化重组蛋白,制备EmEWS多克隆抗体后进行Western blot、实时荧光定量PCR、免疫荧光定位、双荧光测定。结果表明,EmEWS基因长度为1485 bp,编码495个氨基酸,在多房棘球蚴原代细胞中表达量较高,在原头蚴中最低。生物信息学分析表明,细粒棘球蚴EWS与EmEWS相似性高达98.3%,且EmEWS的RBD结构域在绦虫中高度保守。EmEWS重组蛋白大小约为54 ku,制备的多克隆抗体可识别多房棘球绦虫中天然EmEWS蛋白。细胞定位显示EmEWS主要分布在细胞膜上。双荧光试验证明EmEWS可以与结合富含UG的RNA序列结合。综合上述结果,推测EmEWS可能作为转录调控因子参与小RNA的形成,同时有助于核蛋白的运输。

Extraskeletal Ewing sarcoma of the stomach:A rare case report

BACKGROUND Extraskeletal Ewing sarcoma(EES)is a rare and highly malignant small round cell tumor associated with a poor clinical outcome.Ewing sarcoma(ES)involving the stomach is an uncommon presentation and can be easily confused with other small round cell tumors.We herein present a rare case of ES involving the gastric area.CASE SUMMARY We report a case of gastric ES in a 19-year-old female patient who initially presented with a complaint of a tender epigastric mass for 5 d.Contrast-enhanced abdominal computed tomography revealed a soft-tissue-density mass with a diameter of 8.5 cm between the liver and stomach;the mass was connected to the gastric antrum.Then,the mass was surgically excised completely.Upon histopathological,immunophenotype and molecular analysis,the mass was identified to be a primary gastric ES.CONCLUSION EES is an aggressive tumor with poor prognosis.Therefore,early diagnosis and timely intervention are essential for a good prognosis.It is imperative for us to raise awareness about this rare tumor.Surgical resection is still the best treatment option.

HGI联合基于Ewing的HRV诊断预测T2DM患者心脏自主神经病变的价值

目的探讨糖化血红蛋白变异指数(HGI)联合基于Ewing的心率变异性(HRV)诊断预测2型糖尿病(T2DM)患者心脏自主神经病变(DCAN)的价值。方法选取2020年1月至2022年2月在郑州市第九人民医院治疗的T2DM患者140例。以Ewing试验为诊断金标准,分析DCAN和非DCAN患者临床资料、Ewing-HRV诊断参数差异,同时分析发生DCAN的影响因素。结果DCAN发生率为47.14%。DCAN患者糖尿病病程、空腹血糖、糖化血红蛋白(HbA1c)和胰岛素抵抗指数(HOMA-IR)分别为(5.40±1.02)年、(12.01±1.18)mmol/L、(11.43±1.82)%和(6.20±1.02)显著高于非DCAN患者(P<0.05)。HGI为(-0.44±0.19)显著低于非DCAN患者(P<0.05)。伴有DCAN、伴有微血管病变和DCAN患者HGI明显低于单纯T2DM、伴有微血管病变患者(P<0.05);伴有微血管病变患者HGI明显低于单纯T2DM患者(P<0.05)。DCAN患者握力试验时高频功率(HF)、静息状态时LF/HF、Valsalva动作时低频功率(LF)分别为(119.20±12.01)ms^(2)、(3.12±0.83)和(210.10±22.43)ms^(2),显著低于非DCAN患者(P<0.05)。空腹血糖、HbA1c、HOMA-IR、HGI、握力试验时HF、静息状态时LF/HF、Valsalva动作时LF是患者发生DCAN的影响因素(P<0.05)。HGI预测DCAN的ROC曲线下面积为0.882(P<0.05)。HGI联合Ewing-HRV诊断预测DCAN的灵敏性和阴性预测值分别为96.97%和96.92%,明显高于单独应用Ewing-HRV诊断(P<0.05)。结论HGI联合Ewing-HRV诊断预测T2DM患者DCAN有一定应用价值,值得临床使用。

EW-7197对TGF-β1诱导人Tenon囊成纤维细胞增殖和迁移的影响及机制

目的:探讨ALK5抑制剂EW-7197对转化生长因子β1(TGF-β1)诱导的人Tenon囊成纤维细胞(HTFs)增殖和迁移的影响及其机制。方法:采用MTS法检测细胞增殖率,并摸索EW-7197最佳的作用浓度和作用时间。之后将细胞分为3个组,分别为正常对照组、TGF-β1诱导组和TGF-β1+EW-7197处理组,采用Transwell小室观察各组细胞迁移情况,采用Western blot检测各组细胞Fibronectin、α-SMA、p-Smad2、p-Smad3的蛋白表达水平。结果:MTS结果显示,不同处理条件的EW-7197以6.0μmol/L作用24h的细胞增殖率最低(均P<0.01)。Transwell小室实验结果显示,TGF-β1诱导组的细胞迁移数量为228.0±17.0个/视野,明显高于正常对照组(149.0±15.0个/视野)和TGF-β1+EW-7197处理组(46.0±8.0个/视野)(均P<0.01)。Western blot检测结果显示,TGF-β1诱导组细胞中Fibronectin、α-SMA、p-Smad2、p-Smad3相对表达量高于正常对照组和TGF-β1+EW-7197处理组(均P<0.001)。结论:EW-7197能够通过TGF-β/Smad信号通路抑制TGF-β1诱导的HTFs增殖和迁移。

Primary adrenal Ewing sarcoma:A systematic review of the literature

BACKGROUND Ewing sarcoma(ES)is a malignant neoplasm of neuroectodermal origin and is commonly observed in children and young adults.The musculoskeletal system is the main body system impacted and ES is rarely seen in the visceral organs particularly the adrenal gland.AIM To present a comprehensive review of primary adrenal ES,with emphasis on diagnosis,therapy and oncological outcomes.METHODS A systematic review of the literature was performed according to the Preferred Reporting Items for Systematic reviews and Meta-Analyses 2020.PubMed/MEDLINE,EMBASE and Google Scholar bibliographic databases were searched to identify articles from 1989 to 2022 and included patients with ES/primitive neuroectodermal tumor(PNET)of the adrenal gland.PubMed,Google Scholar and EMBASE medical databases were searched,combining the terms“adrenal”,“ES”and“PNET”.Demographic,clinical,pathological and oncological data of patients were analyzed by SPSS version 29.0.RESULTS A total of 52 studies were included for review(47 case reports and 5 case series)with 66 patients reported to have primary adrenal ES.Mean age at diagnosis was 26.4±15.4 years(37.9%males,57.6%females,sex not reported in 3 cases).The most frequent complaint was abdominal/flank pain or discomfort(46.4%)followed by a palpable mass(25.0%),and the average duration of symptoms was 2.6±3.1 mo.The imaging modality of choice was computed tomography scan(81.5%),followed by magnetic resonance imaging(20.4%).Preoperative staging revealed that 17 tumors(27.9%)were metastatic and 14 patients had inferior vena cava or renal vein neoplastic thrombus at initial diagnosis.Open adrenalectomy was performed in the majority of cases(80.0%),of which 27.9%required more extensive resection.Minimally invasive surgery was attempted in 8.2%of tumors.Complete surgical resection was achieved in 89.4%of the patients.Adjuvant therapy was administered to 32 patients,in the form of chemotherapy(62.5%),radiotherapy(3.1%)or combination(34.4%).Median overall survival was 15 mo and 24-mo overall survival was 40.5%.Median disease-free survival was 10 mo and 24-mo disease-free survival was 33.3%.CONCLUSION The significant progress in molecular biology and genetics of ES does not reflect on patient outcomes.ES remains an aggressive tumor with a poor prognosis and high mortality.

An Analysis of Conditional Survival Rates for Ewing Sarcoma Patients

Using data from the Surveillance, Epidemiology, and End Results (SEER) Program based at the National Cancer Institute in the US, conditional survival rates are reported for 1,988 Ewing Sarcoma patients diagnosed during the period 2000-2015. These patients represent the experience of 26.5% of the US population. Specifically, 5-year conditional relative survival rates are calculated for these patients for the first eight years subsequent to diagnosis of their cancer by Extent of Disease (EOD) (Localized, Regional, and Distant as coded by the SEER Program), gender, and age (<18, 18 - 34, and 35+). Findings include showing how the conditional survival rate patterns improve over time and that there are differences by gender, age, and EOD.

Osteosarcoma of the Humerus Developing as Second Malignancy in the Irradiation Field Outside the Primary Tumor: 11 Years after Ewing Sarcoma of the Scapula and 29 Years after Breast Cancer

Purpose: Development of sarcoma is a known late rare negative side effect of radiotherapy. We add two cases to emphasize the need for open-end follow-up and critical evaluation to avoid misinterpretation. Patients, Methods, and Results: Two patients developed osteosarcoma as a second malignancy in the humerus after adjuvant radiotherapy of a primary tumor not directly involving the later affected bone. The first patient had a Ewing sarcoma of the scapula at age 13 years. Though after neoadjuvant chemotherapy the resected specimen showed only fibrotic necrotic areas within clear resection margins, the study group indicated adjuvant radiotherapy in a field including the shoulder joint. At age 24 years she developed an osteosarcoma of the humeral head, which was resected and reconstructed with a proximal humerus endoprosthesis. She is alive without disease at age 32 years. The second patient presented with an osteosarcoma of the proximal humerus 29 years after irradiation for breast cancer including the shoulder joint. The sarcoma was misinterpreted as radiation-induced necrosis and the patient was treated with a reverse shoulder endoprosthesis. Pathologic examination of the resected humeral head then showed a typical osteosarcoma. Two years later the humeral reverse shoulder implant was resected and a proximal humerus tumor prosthesis implanted leaving the original glenosphere. Conclusions: In both cases radiation-induced osteosarcoma developed in bone not affected by the primary cancer. Protecting uninvolved structures must be warranted in the planning of radiotherapy. The long latency between the primary and second cancer mandates long-term—best indefinite—follow-up, as with appropriate treatment of a radiation-induced osteosarcoma good healing rates comparable to those of primary osteosarcoma can still be achieved.