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Influence of REV and ALV-J Co-Infection on Immunologic Function of T Lymphocytes and Histopathology in Broiler Chickens 被引量:6
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作者 GUO Hui-jun, LI Hong-mei, CHENG Zi-qiang , LIU Jian-zhu and CUI Zhi-zhong Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veternary Medicine, Shandong Agricultural University, Tai’an 271018, P.R.China 《Agricultural Sciences in China》 CSCD 2010年第11期1667-1676,共10页
The aim of present investigation is to study the effect of single- and co-infection with REV and ALV-J on T lymphocytes bioactivities and histopathology in broiler chickens. The bioactivities of blood and spleen T lym... The aim of present investigation is to study the effect of single- and co-infection with REV and ALV-J on T lymphocytes bioactivities and histopathology in broiler chickens. The bioactivities of blood and spleen T lymphocytes including lymphoproliferation responses, cytotoxicitic responses, and histopathology of spleen were detected in broiler chickens singly- or co-infected with REV and ALV-J at different days post inoculation and the virus expressions in spleen of infected broiler chickens were detected with immunofluorescence assay (IFA). The results indicated that blood and spleen T lymphocytes proliferation responses and cytotoxicity in broilers infected with REV or/and ALV-J were inhibited in the whole observed period compared with controls. In the co-infected chickens they were highly inhibited than in the single-infected. The histopathology of spleen in infected chickens at 17 and 37 d post inoculation (dpi) indicated that cell interium increased, the numbers of lymphocytes decreased, and the regrowth were destroyed or decreased, especially more significantly at 17 than at 37 dpi. The different numbers of virus were detected in spleen lymphocytes in REV- infected and/or ALV-J-infected chickens. In the spleen of co-infected chicken, both REV and ALV-J were detected and the total numbers of viruses were more than in chickens singly-infected with REV or ALV-J. Thus, the co-effect of REV and ALV-J caused more immunosuppression on T lymphocytes bioactivities in broiler chickens than single-effect of ALV-J or REV, which contributed to the sever histopathology and the product of tumor cells. This study will be helpful for understanding the effect of co-infection with many viruses and control them in poultry. 展开更多
关键词 reticuloendotheliosis virus (REV) j subgroup of avian leukosis virus alv-j T lymphocyte activity histopathology commercial broiler chickens
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鸡IFIT5对J亚群禽白血病病毒在DF-1细胞内复制的影响
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作者 朱丽霖 王后坤 +4 位作者 陈雪阳 刘晶 梁雄燕 方春 杨玉莹 《中国畜牧兽医》 CAS CSCD 北大核心 2023年第1期310-316,共7页
【目的】制备鸡干扰素诱导蛋白含三角形四肽重复5(IFIT5)多克隆抗体,以探究IFIT5对J型禽白血病病毒(ALV-J)在鸡胚成纤维细胞(DF-1)内复制的影响。【方法】以感染ALV-J的鸡脾脏组织细胞提取的RNA反转录获得的cDNA作为模板,对IFIT5基因进... 【目的】制备鸡干扰素诱导蛋白含三角形四肽重复5(IFIT5)多克隆抗体,以探究IFIT5对J型禽白血病病毒(ALV-J)在鸡胚成纤维细胞(DF-1)内复制的影响。【方法】以感染ALV-J的鸡脾脏组织细胞提取的RNA反转录获得的cDNA作为模板,对IFIT5基因进行PCR扩增,利用重组酶将纯化后的PCR产物和线性化载体进行连接,构建重组质粒pET-28a-IFIT5和pcDNA5-flag-IFIT5并测序。将测序正确的重组质粒pET-28a-IFIT5转化大肠杆菌BL21感受态细胞进行诱导表达,表达的融合蛋白His-IFIT5纯化后免疫6周龄BALB/c雌鼠制备多克隆抗体,通过Western blotting鉴定抗体的特异性,利用间接ELISA测定抗体的效价。将测序正确的重组质粒pcDNA5-flag-IFIT5转染DF-1细胞,24 h后接种ALV-J,通过Western blotting检测DF-1细胞中过表达IFIT5对ALV-J复制的影响。【结果】试验成功扩增到大小为1 440 bp的IFIT5基因,并成功构建重组质粒pET-28a-IFIT5和pcDNA5-flag-IFIT5。pET-28a-IFIT5可以表达约56 ku的可溶性蛋白His-IFIT5,Western blotting结果显示,制备的鼠抗IFIT5蛋白多克隆抗体能特异性识别DF-1细胞中过表达的IFIT5蛋白,效价为1∶32 000。pcDNA5-flag-IFIT5能在DF-1细胞中高效表达,且在DF-1细胞中过表达IFIT5可以促进ALV-J复制。【结论】本研究制备的鼠抗IFIT5蛋白多克隆抗体具有较高的反应性和特异性;在DF-1细胞中过表达IFIT5可以促进ALV-J复制。本试验结果为深入研究IFIT5蛋白的生物学功能提供参考。 展开更多
关键词 IFIT5基因 j型禽白血病病毒(alv-j) 原核表达 多克隆抗体
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1株J亚群禽白血病病毒env基因克隆及生物信息学分析 被引量:1
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作者 陈玫婷 郑从森 +6 位作者 梁泽贤 林巧儿 常传哲 周俊 冯军 李林林 覃丽梅 《中国畜牧兽医》 CAS CSCD 北大核心 2023年第5期1785-1795,共11页
【目的】通过生物信息学方法分析1株禽白血病病毒(Avian Leukosis virus,ALV)的囊膜蛋白env的分子特征。【方法】利用鸡成纤维细胞(DF-1)培养疑似禽白血病病鸡的血清样品,通过ELISA和实时荧光定量PCR鉴定ALV亚群。对env基因PCR产物进行... 【目的】通过生物信息学方法分析1株禽白血病病毒(Avian Leukosis virus,ALV)的囊膜蛋白env的分子特征。【方法】利用鸡成纤维细胞(DF-1)培养疑似禽白血病病鸡的血清样品,通过ELISA和实时荧光定量PCR鉴定ALV亚群。对env基因PCR产物进行测序,利用SeqMan拼接env基因。将该env基因与国内外多个ALV亚群进行序列比对及遗传进化分析,并借助多种生物信息学软件对env蛋白进行分析,包括理化性质、亲/疏水性、跨膜区、信号肽、糖基化位点、磷酸化位点、乙酰化位点、二级结构、三级结构、抗原决定簇和B细胞抗原表位预测。【结果】ALV-J毒株的env基因全长1719 bp,获得GenBank登录号为OP837418,其与J亚群ALV位于同一进化分支,相似性为89.3%~94.8%。该env蛋白有2个跨膜区,是由572个氨基酸组成的不稳定的亲水蛋白,不稳定系数预测值为43.49,总平均亲水性为―0.201;该env蛋白为非分泌蛋白,无信号肽,有17个N-糖基化修饰位点、77个O-糖基化修饰位点、42个磷酸化修饰位点、21个潜在抗原决定簇和9个连续碱基数超过10个的B细胞线性表位。该env蛋白的二级结构中无规则卷曲占比最大,为38.29%。【结论】env基因是导致ALV遗传变异的关键基因,其编码的囊膜蛋白env具有不稳定性,存在多个蛋白修饰位点和抗原表位。 展开更多
关键词 禽白血病病毒(ALV) j亚群 ENV基因 生物信息学
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Different quasispecies with great mutations hide in the same subgroup J field strain of avian leukosis virus 被引量:6
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作者 MAO YaQing LI WeiHua +2 位作者 DONG Xuan LIU JinHua ZHAO Peng 《Science China(Life Sciences)》 SCIE CAS 2013年第5期414-420,共7页
Blood samples were collected from a local strain of chickens associated with serious tumor cases in Shandong Province. The samples were inoculated into chicken embryo fibroblast and DF-1 cells for virus isolation and ... Blood samples were collected from a local strain of chickens associated with serious tumor cases in Shandong Province. The samples were inoculated into chicken embryo fibroblast and DF-1 cells for virus isolation and identification, respectively. The inoculated cells were screened for three common chicken tumor viruses. Nine strains of avian leukosis virus subgroup J (ALV-J) were identified, and were designated LY1201-LYI209. The env gene from the LY1201 strain was amplified and cloned. All nine resultant env clones (clones 01-09) were sequenced, and the gp85 and gp37 amino acid regions were subjected to homology analysis. Clones 01 and 03 had 10 amino acid deletions in the gp85 region compared to the other seven clones, suggesting that at least two quasispecies with obvious mutations coexist in the same field strain. Among these nine clones, three had identical gp85 and gp37 sequences, and were recognized as the dominant LY1201 quasispecies. The amino acid sequence homology of gp37 and gp85 among the nine clones was 98.5%-100.0% and 96.6%-100.0% respectively, suggesting that the gp85 region of the env gene can better display the quasispecies diversity of ALV-J than gp37. 展开更多
关键词 avian leukosis virus subgroup j(alv-j) gp85 gp37 quasispecies diversity
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Semen extracellular vesicles mediate vertical transmission of subgroup J avian leukosis virus
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作者 Liqin Liao Weiguo Chen +9 位作者 Xiangyu Zhang Huanmin Zhang Aijun Li Yiming Yan Zi Xie Hongxing Li Wencheng Lin Jingyun Ma Xinheng Zhang Qingmei Xie 《Virologica Sinica》 SCIE CAS CSCD 2022年第2期284-294,共11页
Subgroup J avian leukosis virus(ALV-J) is a highly oncogenic retrovirus that has been devastating the global poultry industry since the late 1990s. The major infection model of ALV-J is vertical transmission, which is... Subgroup J avian leukosis virus(ALV-J) is a highly oncogenic retrovirus that has been devastating the global poultry industry since the late 1990s. The major infection model of ALV-J is vertical transmission, which is responsible for the congenital infection of progeny from generation to generation. Increasing evidence has suggested that extracellular vesicles(EVs) derived from virus-infected cells or biological fluids have been thought to be vehicles of transmission for viruses. However, the role of EVs in infection and transmission of ALV-J remains obscure. In the present study, semen extracellular vesicles(SE) were isolated and purified from ALV-J-infected rooster seminal plasma(SE-ALV-J), which was shown to contain ALV-J genomic RNA and partial viral proteins, as determined by RNA sequencing, reverse transcription-quantitative PCR and Western blotting. Furthermore, SE-ALV-J was proved to be able to transmit ALV-J infection to host cells and establish productive infection.More importantly, artificial insemination experiments showed that SE-ALV-J transmitted ALV-J infection to SPF hens, and subsequently mediated vertical transmission of ALV-J from the SPF hens to the progeny chicks. Taken together, the results of the present study suggested that ALV-J utilized host semen extracellular vesicles as a novel means for vertical transmission, enhancing our understanding on mechanisms underlying ALV-J transmission. 展开更多
关键词 Semen extracellular vesicles(SE) subgroup j avian leukosis virus(alv-j) INFECTION Vertical transmission
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我国鸡群J亚群白血病流行的过去、现在和将来及其防控——对我国动物疫病防控的启示 被引量:12
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作者 崔治中 《现代畜牧兽医》 2015年第1期30-32,共3页
禽白血病病毒(avian leukosis virus,ALV)是一种能引起禽类多种类型肿瘤的反转录病毒,包括A-J等10个亚群。其中J亚群禽白血病病毒(Subgroup J of avian leukosis virus,ALV-J)是20世纪80年代末Payne等首先从肉鸡中分离鉴定出来的亚群。... 禽白血病病毒(avian leukosis virus,ALV)是一种能引起禽类多种类型肿瘤的反转录病毒,包括A-J等10个亚群。其中J亚群禽白血病病毒(Subgroup J of avian leukosis virus,ALV-J)是20世纪80年代末Payne等首先从肉鸡中分离鉴定出来的亚群。自1999年我国首次分离ALV-J以来,ALV-J已从肉用鸡群向蛋用型鸡群和地方品系鸡群传播。本文对我国鸡群J亚群白血病流行的过去、现在和将来及其防控作以简要介绍,以期对我国动物疫病防控产生新的启示。 展开更多
关键词 禽白血病病毒 j亚群 疫病防控
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绿壳蛋鸡MDV与J亚群外源性ALV混合感染病原的鉴定及主要基因序列分析 被引量:4
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作者 张喜懿 温贵兰 +3 位作者 文明 欧德渊 陈广 张小波 《黑龙江畜牧兽医》 CAS 北大核心 2021年第13期68-74,147,共8页
为了确定贵州省某养殖场病死绿壳蛋鸡的发病原因,试验对送检的病死鸡进行剖检观察,无菌采集病变组织提取核酸并进行基因克隆及测序,分析同源性并构建遗传进化树。结果表明:3只病死鸡剖检可见脾脏肿大、质脆;肝脏肿大,有肿瘤结节;腺胃肿... 为了确定贵州省某养殖场病死绿壳蛋鸡的发病原因,试验对送检的病死鸡进行剖检观察,无菌采集病变组织提取核酸并进行基因克隆及测序,分析同源性并构建遗传进化树。结果表明:3只病死鸡剖检可见脾脏肿大、质脆;肝脏肿大,有肿瘤结节;腺胃肿大。马立克病病毒(Marek’s disease virus,MDV)核酸检测仅MDV meq基因出现与预期片段大小相符的特异性扩增条带;禽白血病病毒(Avian leukosis virus,ALV)分离鉴定仅ALV J gp85基因出现与预期片段大小相符的特异性扩增条带;将目的片段克隆至pMD19-T载体上,经测序获得3个MDV meq基因片段和3个ALV J gp85基因片段,核苷酸序列大小分别约为1020,930 bp;获得的3株MDV毒株和3株ALV毒株与GenBank中相应参考毒株的核苷酸序列同源性分别为98.6%~100%、46.1%~99.6%,分别与参考毒株G2株和HPRS103株在同一分支上,亲缘关系较近。说明贵州省某养殖场鸡只的死亡原因是MDV与J亚群外源性ALV混合感染所致。 展开更多
关键词 绿壳蛋鸡 马立克病病毒(MDV) j亚群外源性禽白血病病毒(alv-j) 混合感染 病原鉴定 序列分析
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2株J亚群禽白血病病毒毒株的分离鉴定及全基因组序列分析 被引量:1
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作者 郭雨欣 游广炬 +3 位作者 李晓齐 高丽 郑世军 王永强 《中国兽医杂志》 CAS 北大核心 2021年第6期7-12,18,共7页
为了解我国J亚群禽白血病病毒(ALV)的流行特点和进化趋势,本试验从河北某鸡场疑似禽白血病的临床发病鸡的病料组织中,分离得到2株ALV-J毒株,分别命名为CAU2019和CAU2020。通过聚合酶链式反应(PCR)技术分段扩增分离株的全病毒基因组,连接... 为了解我国J亚群禽白血病病毒(ALV)的流行特点和进化趋势,本试验从河北某鸡场疑似禽白血病的临床发病鸡的病料组织中,分离得到2株ALV-J毒株,分别命名为CAU2019和CAU2020。通过聚合酶链式反应(PCR)技术分段扩增分离株的全病毒基因组,连接pEASY-Blunt Zero载体后进行序列测定,并对获得的基因组序列与ALV各亚群毒株序列进行序列比对及遗传进化分析。结果表明,2个分离株与ALV各亚群毒株的gag和pol段基因相对保守,而LTR、gp85及gp37段基因变异较大。此外,CAU2019在3′UTR处有2段长度为30 bp和11 bp的不连续的碱基缺失,CAU2020在3′UTR处有1段长度为29 bp的碱基缺失。由gp85和LTR基因构建的遗传进化树可知,2个分离株在遗传关系上与J亚群毒株JS16JH9亲缘关系最近。结果表明本试验分离的2株病毒均为J亚群禽白血病病毒毒株,并且为国内ALV-J流行株的突变株。 展开更多
关键词 禽白血病病毒 j亚群 分离鉴定 全基因组序列分析
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J亚群禽白血病病毒致病机理研究进展 被引量:8
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作者 张帆帆 涂凌云 +6 位作者 李海琴 曾艳兵 康昭风 谭美芳 谭佳 方绍培 杨群 《中国兽医学报》 CAS CSCD 北大核心 2021年第11期2288-2293,共6页
J亚群禽白血病病毒(ALV-J)是一种高致癌性反转录病毒,可引发髓性细胞性白血病及各种肿瘤性疾病,并产生免疫抑制,易继发细菌及其他病毒感染。长久以来,禽白血病给养禽业造成了巨大的经济损失,且没有有效的预防和治疗手段,仅依靠禽白血病... J亚群禽白血病病毒(ALV-J)是一种高致癌性反转录病毒,可引发髓性细胞性白血病及各种肿瘤性疾病,并产生免疫抑制,易继发细菌及其他病毒感染。长久以来,禽白血病给养禽业造成了巨大的经济损失,且没有有效的预防和治疗手段,仅依靠禽白血病病毒的净化,仍不能有效控制禽白血病的流行和蔓延。本文主要阐述ALV-J病毒的感染、复制、致瘤及免疫抑制机制、宿主天然抗病毒过程及其分子机制,对需要进一步深入研究的目标和内容提出了展望。 展开更多
关键词 j亚群禽白血病病毒(alv-j) 致病机理 免疫抑制 抗病毒免疫
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