Decreased expression of brain-derived neurotrophic factor（BDNF） plays an important role in the pathogenesis of Alzheimer＇s disease, and a typical pathological change in Alzheimer＇s disease is neurofibrillary tangl...Decreased expression of brain-derived neurotrophic factor（BDNF） plays an important role in the pathogenesis of Alzheimer＇s disease, and a typical pathological change in Alzheimer＇s disease is neurofibrillary tangles caused by hyperphosphorylation of tau. An in vivo model of Alzheimer＇s disease was developed by injecting okadaic acid（2 μL） and exogenous BDNF（2 μL） into the hippocampi of adult male Wister rats. Spatial learning and memory abilities were assessed using the Morris water maze. The expression levels of protein phosphatase 2 A（PP2 A）, PP2 Ac-Yp307, p-tau（Thr231）, and p-tau（Ser396/404） were detected by western blot assay. The expression levels of BDNF, TrkB, and synaptophysin mRNA were measured by quantitative real-time polymerase chain reaction. Our results indicated that BDNF expression was suppressed in the hippocampus of OA-treated rats, which resulted in learning and memory deficits. Intra-hippocampal injection of BDNF attenuated this OA-induced cognitive impairment. Finally, our findings indicated an involvement of the PI3 K/GSK-3β/AKT pathway in the mechanism of BDNF in regulating cognitive function. These results indicate that BDNF has beneficial effect on Alzheimer＇s disease, and highlight the potential of BDNF as a drug target for treatment of Alzheimer＇s disease.展开更多
In this study, several general y reported exogenous phenolic acids were selected as regents for potting experiments, aiming at revealing the effects of differ-ent concentrations of exogenous phenolic acids on the grow...In this study, several general y reported exogenous phenolic acids were selected as regents for potting experiments, aiming at revealing the effects of differ-ent concentrations of exogenous phenolic acids on the growth and development of soybean seedlings. The growth and development indicators of soybean seedlings treated with five different concentrations of exogenous phenolic acids, such as plant height, leaf area, root nodule number and root-shoot ratio, were determined and compared. According to the experimental results, three exogenous phenolic acids with different concentrations exhibited slight effects on plant height of potted soy-bean seedlings. The leaf area of potted soybean seedlings treated with 1.0 g/kg benzoic acid and cinnamic acid varied extremely significantly compared with the control. Root nodule number of potted soybean seedlings treated with 1.0 g/kg ben-zoic acid and vanil ic acid increased remarkably, but 1.0 g/kg cinnamic acid had lit-tle effects on root nodule number. When the concentration of phenolic acids was 1.0 g/kg, three phenolic acids exhibited the maximum effects on root-shoot ratio of potted soybean seedlings. Overal , low concentrations of exogenous phenolic acids exhibited no significant effects on the growth and development of potted soybean seedlings; high concentrations of exogenous phenolic acids inhibited the growth and development of potted soybean shoots, increased root nodule number and improved root-shoot ratio.展开更多
Salicylic acid(SA)is an endogenous regulatorymolecule for plant flowering and thermogene-sis,and has been reported as a signal moleculeof plant disease resistance in the plant-pathogen interaction.Exogenous SA is capa...Salicylic acid(SA)is an endogenous regulatorymolecule for plant flowering and thermogene-sis,and has been reported as a signal moleculeof plant disease resistance in the plant-pathogen interaction.Exogenous SA is capableof inducing disease resistance in a wide range ofplants.This study was to evaluate the poten-tial for SA to induce resistance of rice seedlings展开更多
Since the azo dyes widely used in textile-dyeing industry are toxic, highly persistent, and ubiquitously distributed in the environment, their treatment efficiencies and fade in ecosystems have received worldwide atte...Since the azo dyes widely used in textile-dyeing industry are toxic, highly persistent, and ubiquitously distributed in the environment, their treatment efficiencies and fade in ecosystems have received worldwide attention. Due to the microbes play important roles in azo-dye degradation, exogenous nutrient addition is used as a promising biostimulation strategy to improve the treatment efficiencies of azo-dye wastewater. However, little is known about the effects of different kinds of exogenous nutrients on the azo-dye wastewater treatment directly. Here, three kinds of common nutrients, glucose, starch and yeast extract, were added to the sequencing batch reactors （SBR） for the treatment of wastewater containing acid red 73 to compare the biostimulation efficiencies by investigating the dye＇s removal efficiencies and the changes of related water qualities. Our results showed that the reactors added yeast extract had highest removal efficiencies and chemical oxygen demand （COD） as well, followed by glucose and starch. The removal rates of acid red 73 and COD were 90.63% and 8713% in the reactors added yeast extract, respectively, while 86.49% and 78.4% in those with glucose and 85.38% and 75.2% in those with starch. This study provided some useful information for the biostimulation strategy of azo-dye wastewater treatment and preliminarily suggested that yeast extract would be the optimal choice.展开更多
Phosphorus bioavailability has long been a recurring problem in tropical acid soils. A pot experiment was carried out during three (3) successive rice production cycles at Adiopodoumé to evaluate the response of ...Phosphorus bioavailability has long been a recurring problem in tropical acid soils. A pot experiment was carried out during three (3) successive rice production cycles at Adiopodoumé to evaluate the response of the NERICA 5 rice accession to various doses of calcium, magnesium and phosphorous. The experiment was conducted using a randomized split-plot design. The interactive effects of calcium carbonate (0, 25, 50 and 75 kg·Ca·ha<sup>-1</sup>) and magnesium sulfate (0, 25, 50 and 75 kg·Mg·ha<sup>-1</sup>) and Togo natural phosphate (0, 25, 50 and 75 kg·P·ha<sup>-1</sup>) were determined at each production cycle. The results showed that single-dose natural phosphate supplementation for three cropping cycles resulted in an average enrichment of around 2 mg·P·kg<sup>-1</sup> after each trial following its continuous dissolution, with an increase in DSP (33.31% to 70.52%). The study revealed one strategy for managing and enhancing native P with cations and another for exogenous P: there would be a synergy of Ca/Mg on native P, whereas an antagonism would characterize the two parameters in phosphate fertilization.展开更多
文摘Decreased expression of brain-derived neurotrophic factor（BDNF） plays an important role in the pathogenesis of Alzheimer＇s disease, and a typical pathological change in Alzheimer＇s disease is neurofibrillary tangles caused by hyperphosphorylation of tau. An in vivo model of Alzheimer＇s disease was developed by injecting okadaic acid（2 μL） and exogenous BDNF（2 μL） into the hippocampi of adult male Wister rats. Spatial learning and memory abilities were assessed using the Morris water maze. The expression levels of protein phosphatase 2 A（PP2 A）, PP2 Ac-Yp307, p-tau（Thr231）, and p-tau（Ser396/404） were detected by western blot assay. The expression levels of BDNF, TrkB, and synaptophysin mRNA were measured by quantitative real-time polymerase chain reaction. Our results indicated that BDNF expression was suppressed in the hippocampus of OA-treated rats, which resulted in learning and memory deficits. Intra-hippocampal injection of BDNF attenuated this OA-induced cognitive impairment. Finally, our findings indicated an involvement of the PI3 K/GSK-3β/AKT pathway in the mechanism of BDNF in regulating cognitive function. These results indicate that BDNF has beneficial effect on Alzheimer＇s disease, and highlight the potential of BDNF as a drug target for treatment of Alzheimer＇s disease.
基金Supported by the Scientific Research Fund for Innovative Research Team of Colleges and Universities in Liaoning Province(2009T088)~~
文摘In this study, several general y reported exogenous phenolic acids were selected as regents for potting experiments, aiming at revealing the effects of differ-ent concentrations of exogenous phenolic acids on the growth and development of soybean seedlings. The growth and development indicators of soybean seedlings treated with five different concentrations of exogenous phenolic acids, such as plant height, leaf area, root nodule number and root-shoot ratio, were determined and compared. According to the experimental results, three exogenous phenolic acids with different concentrations exhibited slight effects on plant height of potted soy-bean seedlings. The leaf area of potted soybean seedlings treated with 1.0 g/kg benzoic acid and cinnamic acid varied extremely significantly compared with the control. Root nodule number of potted soybean seedlings treated with 1.0 g/kg ben-zoic acid and vanil ic acid increased remarkably, but 1.0 g/kg cinnamic acid had lit-tle effects on root nodule number. When the concentration of phenolic acids was 1.0 g/kg, three phenolic acids exhibited the maximum effects on root-shoot ratio of potted soybean seedlings. Overal , low concentrations of exogenous phenolic acids exhibited no significant effects on the growth and development of potted soybean seedlings; high concentrations of exogenous phenolic acids inhibited the growth and development of potted soybean shoots, increased root nodule number and improved root-shoot ratio.
文摘Salicylic acid(SA)is an endogenous regulatorymolecule for plant flowering and thermogene-sis,and has been reported as a signal moleculeof plant disease resistance in the plant-pathogen interaction.Exogenous SA is capableof inducing disease resistance in a wide range ofplants.This study was to evaluate the poten-tial for SA to induce resistance of rice seedlings
基金supported by the National Natural Science Foundation of China(51308133)Guangdong Provincial National Natural Science Foundation(2014A030308019)+1 种基金Science and Technology Planning Project of Guangdong Province(2015A020215016)the support of Guangdong Institute of Microbiology
文摘Since the azo dyes widely used in textile-dyeing industry are toxic, highly persistent, and ubiquitously distributed in the environment, their treatment efficiencies and fade in ecosystems have received worldwide attention. Due to the microbes play important roles in azo-dye degradation, exogenous nutrient addition is used as a promising biostimulation strategy to improve the treatment efficiencies of azo-dye wastewater. However, little is known about the effects of different kinds of exogenous nutrients on the azo-dye wastewater treatment directly. Here, three kinds of common nutrients, glucose, starch and yeast extract, were added to the sequencing batch reactors （SBR） for the treatment of wastewater containing acid red 73 to compare the biostimulation efficiencies by investigating the dye＇s removal efficiencies and the changes of related water qualities. Our results showed that the reactors added yeast extract had highest removal efficiencies and chemical oxygen demand （COD） as well, followed by glucose and starch. The removal rates of acid red 73 and COD were 90.63% and 8713% in the reactors added yeast extract, respectively, while 86.49% and 78.4% in those with glucose and 85.38% and 75.2% in those with starch. This study provided some useful information for the biostimulation strategy of azo-dye wastewater treatment and preliminarily suggested that yeast extract would be the optimal choice.
文摘Phosphorus bioavailability has long been a recurring problem in tropical acid soils. A pot experiment was carried out during three (3) successive rice production cycles at Adiopodoumé to evaluate the response of the NERICA 5 rice accession to various doses of calcium, magnesium and phosphorous. The experiment was conducted using a randomized split-plot design. The interactive effects of calcium carbonate (0, 25, 50 and 75 kg·Ca·ha<sup>-1</sup>) and magnesium sulfate (0, 25, 50 and 75 kg·Mg·ha<sup>-1</sup>) and Togo natural phosphate (0, 25, 50 and 75 kg·P·ha<sup>-1</sup>) were determined at each production cycle. The results showed that single-dose natural phosphate supplementation for three cropping cycles resulted in an average enrichment of around 2 mg·P·kg<sup>-1</sup> after each trial following its continuous dissolution, with an increase in DSP (33.31% to 70.52%). The study revealed one strategy for managing and enhancing native P with cations and another for exogenous P: there would be a synergy of Ca/Mg on native P, whereas an antagonism would characterize the two parameters in phosphate fertilization.
