Expanded bed adsorption(EBA),a promising and practical separation technique,has been widely studied in the past two decades.The development of adsorbents for EBA process is a challenging course,with the special design...Expanded bed adsorption(EBA),a promising and practical separation technique,has been widely studied in the past two decades.The development of adsorbents for EBA process is a challenging course,with the special design and preparation according to the target molecules and specific expanded bed systems.Many types of supporting matrices for expanded bed adsorbents have been developed,and their preparation methods are being consummated gradually.These matrices are activated and then coupled with ligands to form functionalized adsorbents,including ion-exchange adsorbents,affinity adsorbents,mixed mode adsorbents,hydrophobic charge induction chromatography adsorbents and others.In this review,the preparation of the matrices for EBA process is summa-rized,and the coupling of ligands to the matrices to prepare functionalized adsorbents is discussed as well.展开更多
末水条斑紫菜以组织捣碎机粉碎,在10 mmol/L磷酸盐缓冲液(p H 6.8)中反复冻融3次,收集上清液,通过膨化床疏水层析技术分离R-藻红蛋白(R-PE),剩余残渣用于硫酸多糖提取。使用膨化柱分离,粗提液中约17%的藻红蛋白可以被回收,DEAE-Sepharos...末水条斑紫菜以组织捣碎机粉碎,在10 mmol/L磷酸盐缓冲液(p H 6.8)中反复冻融3次,收集上清液,通过膨化床疏水层析技术分离R-藻红蛋白(R-PE),剩余残渣用于硫酸多糖提取。使用膨化柱分离,粗提液中约17%的藻红蛋白可以被回收,DEAE-Sepharose离子交换树脂纯化后,约12%的R-藻红蛋白得到回收,光谱纯度大于3.2,纯化得率为0.66 mg/g鲜紫菜。吸收光谱、荧光发射光谱以及凝胶电泳均显示该藻红蛋白属于典型三峰型R-藻红蛋白。利用藻红蛋白提取后的残渣,每克条斑紫菜可回收到30.5 mg的粗多糖,其中硫酸基含量为21.8%。实验证明,微波辅助抽提与热水浸提对硫酸多糖得率无显著影响。纯化的R-藻红蛋白与紫菜硫酸多糖可用于食品、化妆品添加,或药品、保健品的开发。本文对探索次等紫菜生物质的综合、高值化利用,进一步促进我国相关产品的开发与应用具有重要意义。展开更多
目的采用一步吸附及分步解吸的柱层析技术——扩张床吸附(expanded bed adsorption,EBA)技术,从原料血浆中分离多个有效组分,从而提高血浆的综合利用率。方法以琼脂糖(碳化钨)-DEAE为吸附介质,将原料血浆按一定流速流经层析柱,并使待分...目的采用一步吸附及分步解吸的柱层析技术——扩张床吸附(expanded bed adsorption,EBA)技术,从原料血浆中分离多个有效组分,从而提高血浆的综合利用率。方法以琼脂糖(碳化钨)-DEAE为吸附介质,将原料血浆按一定流速流经层析柱,并使待分离组分吸附于介质上,通过不同的缓冲液组成、洗脱流速、洗脱体积分步分离有效组分,并初步探讨工艺放大过程中的关键因素。结果 EBA技术可从原料血浆中依次分离出白蛋白、免疫球蛋白、纤维蛋白原、α-1蛋白酶抑制剂4种蛋白;该技术在工艺放大过程中,柱高及洗脱流速是影响分离效果的关键因素;该技术可去除部分病毒,使病毒滴度降低约一个数量级。结论一步柱层析技术具有血浆综合利用度高、易操作、操作周期短、成本低、可去除病毒等优势,其有望成为传统低温乙醇工艺的有效替代技术,应用于工业化生产。展开更多
基金Supported by the National Natural Science Foundation of China （20876139, 20776129） and the National Basic Research Program of China （2007CB707805）.
文摘Expanded bed adsorption(EBA),a promising and practical separation technique,has been widely studied in the past two decades.The development of adsorbents for EBA process is a challenging course,with the special design and preparation according to the target molecules and specific expanded bed systems.Many types of supporting matrices for expanded bed adsorbents have been developed,and their preparation methods are being consummated gradually.These matrices are activated and then coupled with ligands to form functionalized adsorbents,including ion-exchange adsorbents,affinity adsorbents,mixed mode adsorbents,hydrophobic charge induction chromatography adsorbents and others.In this review,the preparation of the matrices for EBA process is summa-rized,and the coupling of ligands to the matrices to prepare functionalized adsorbents is discussed as well.
文摘目的采用一步吸附及分步解吸的柱层析技术——扩张床吸附(expanded bed adsorption,EBA)技术,从原料血浆中分离多个有效组分,从而提高血浆的综合利用率。方法以琼脂糖(碳化钨)-DEAE为吸附介质,将原料血浆按一定流速流经层析柱,并使待分离组分吸附于介质上,通过不同的缓冲液组成、洗脱流速、洗脱体积分步分离有效组分,并初步探讨工艺放大过程中的关键因素。结果 EBA技术可从原料血浆中依次分离出白蛋白、免疫球蛋白、纤维蛋白原、α-1蛋白酶抑制剂4种蛋白;该技术在工艺放大过程中,柱高及洗脱流速是影响分离效果的关键因素;该技术可去除部分病毒,使病毒滴度降低约一个数量级。结论一步柱层析技术具有血浆综合利用度高、易操作、操作周期短、成本低、可去除病毒等优势,其有望成为传统低温乙醇工艺的有效替代技术,应用于工业化生产。
