OBJECTIVE: To investigate the effect of Daiqin phlegm-expelling pill, prepared with Traditional Chinese Medicine(TCM), on the development of inflammation in Sprague-Dawley(SD) rats with chronic obstructive pulmonary d...OBJECTIVE: To investigate the effect of Daiqin phlegm-expelling pill, prepared with Traditional Chinese Medicine(TCM), on the development of inflammation in Sprague-Dawley(SD) rats with chronic obstructive pulmonary disease(COPD) induced by lipopolysaccharide(LPS) and smoke, and to identity the possible underlying mechanism.METHODS: Sixty male rats were divided into 6 groups(healthy control group, untreated group,Daiqin phlegm-expelling pill low, middle and high dose, and ambroxol hydrochloride tablet group).COPD was established in SD rats by sootiness and tracheal instillation with LPS. The rats were treated with Daiqin phlegm-expelling pill at the indicated doses for 28 d, the inflammatory cells in bronchoalveolar lavage fluid(BALF), the concentration of tumor necrosis factor-α(TNF-α), interleukin(IL)-8 and IL-6 in blood and the inflammation in lung were evaluated.RESULTS: The number of inflammatory cells in the BALF and TNF-ficantly redα, IL-8 and IL-6 level in plasma were signiuced in rats with COPD when treated with Daiqin phlegm-expelling pill or ambroxol hydrochloride tablet, compared with those without any treatment. The Daiqin phlegm-expelling pill treated rats with COPD had a attenuated inflammation in lung tissue, compared with the untreated group.CONCLUSION: Daiqin phlegm-expelling pill can significantly restrain the airway inflammation in rats with LPS-smoke induced COPD.展开更多
The objective of this study was to compare the effect of linseed and canola expeller on average daily weight gain(ADG),concentrate intakes,incidence of diarrhea,serum haptoglobin,interleukin(IL)-1,and resolvin-E1 in f...The objective of this study was to compare the effect of linseed and canola expeller on average daily weight gain(ADG),concentrate intakes,incidence of diarrhea,serum haptoglobin,interleukin(IL)-1,and resolvin-E1 in female Holstein calves from birth to weaning.A sample size of 20 calves per group was calculated and were randomly allocated at the time of birth.Linseed group(LIN)was fed a starter with linseed expeller,while canola group(CAN)received a similar concentrate,but with canola expeller.Both expellers were included at a rate of 25%dry matter(DM)basis of the starter.Pasteurized waste milk was fed twice a day until weaning.Calves were weighed at birth,30,and 60 d of age.Starter intake was evaluated daily from 5 to 60 d.A blood sample was obtained at birth,14,28,35,and 49 d of age,and bovine serum resolvin-El,haptoglobin,and IL-1 were assayed by commercial enzyme-linked immunosorbent assay(ELISA)kits.Incidence of diarrhea and the duration of the events were also recorded.The effect of the interaction group by time on body weight(BW)and starter intake was not significant(P>0.05).Average daily gains(ADG)from 0 to 60 d for CAN and LIN groups were 0.680 and 0.675 kg/d(P>0.05),respectively.Incidences of diarrhea were 25%and 45%for CAN and LIN groups,respectively(P=0.18).LIN group had greater concentrations of IL-1 at d 21,haptoglobin at d 7,and resolving-El at d 14 and 49 than CAN group,respectively.It is concluded that BW at weaning,ADG,and concentrate intakes were not different between groups fed starters containing linseed or canola expeller(25%inclusion).The concentrations of cytokines and haptoglobin were the greatest in LIN group.展开更多
On August 30, the Graduate School of Huazhong University of Science and Technology announced it would expel students who were taking too long to complete advanced degrees. The relevant regulations say completing a mas...On August 30, the Graduate School of Huazhong University of Science and Technology announced it would expel students who were taking too long to complete advanced degrees. The relevant regulations say completing a master’s degree shouldn’t take longer than four years and a doctor’s degree eight.展开更多
基金Natural Science Foundation of Jiangsu Province(No.BK20171098)Jiangsu Province 12th Five-Year Plan for the construction of Key Traditional Chinese Medicine Discipline(No.JS1302)Project of Affiliated Hospital of Nanjing University of Traditional Chinese Medicine(No.Y17002)
文摘OBJECTIVE: To investigate the effect of Daiqin phlegm-expelling pill, prepared with Traditional Chinese Medicine(TCM), on the development of inflammation in Sprague-Dawley(SD) rats with chronic obstructive pulmonary disease(COPD) induced by lipopolysaccharide(LPS) and smoke, and to identity the possible underlying mechanism.METHODS: Sixty male rats were divided into 6 groups(healthy control group, untreated group,Daiqin phlegm-expelling pill low, middle and high dose, and ambroxol hydrochloride tablet group).COPD was established in SD rats by sootiness and tracheal instillation with LPS. The rats were treated with Daiqin phlegm-expelling pill at the indicated doses for 28 d, the inflammatory cells in bronchoalveolar lavage fluid(BALF), the concentration of tumor necrosis factor-α(TNF-α), interleukin(IL)-8 and IL-6 in blood and the inflammation in lung were evaluated.RESULTS: The number of inflammatory cells in the BALF and TNF-ficantly redα, IL-8 and IL-6 level in plasma were signiuced in rats with COPD when treated with Daiqin phlegm-expelling pill or ambroxol hydrochloride tablet, compared with those without any treatment. The Daiqin phlegm-expelling pill treated rats with COPD had a attenuated inflammation in lung tissue, compared with the untreated group.CONCLUSION: Daiqin phlegm-expelling pill can significantly restrain the airway inflammation in rats with LPS-smoke induced COPD.
文摘The objective of this study was to compare the effect of linseed and canola expeller on average daily weight gain(ADG),concentrate intakes,incidence of diarrhea,serum haptoglobin,interleukin(IL)-1,and resolvin-E1 in female Holstein calves from birth to weaning.A sample size of 20 calves per group was calculated and were randomly allocated at the time of birth.Linseed group(LIN)was fed a starter with linseed expeller,while canola group(CAN)received a similar concentrate,but with canola expeller.Both expellers were included at a rate of 25%dry matter(DM)basis of the starter.Pasteurized waste milk was fed twice a day until weaning.Calves were weighed at birth,30,and 60 d of age.Starter intake was evaluated daily from 5 to 60 d.A blood sample was obtained at birth,14,28,35,and 49 d of age,and bovine serum resolvin-El,haptoglobin,and IL-1 were assayed by commercial enzyme-linked immunosorbent assay(ELISA)kits.Incidence of diarrhea and the duration of the events were also recorded.The effect of the interaction group by time on body weight(BW)and starter intake was not significant(P>0.05).Average daily gains(ADG)from 0 to 60 d for CAN and LIN groups were 0.680 and 0.675 kg/d(P>0.05),respectively.Incidences of diarrhea were 25%and 45%for CAN and LIN groups,respectively(P=0.18).LIN group had greater concentrations of IL-1 at d 21,haptoglobin at d 7,and resolving-El at d 14 and 49 than CAN group,respectively.It is concluded that BW at weaning,ADG,and concentrate intakes were not different between groups fed starters containing linseed or canola expeller(25%inclusion).The concentrations of cytokines and haptoglobin were the greatest in LIN group.
文摘On August 30, the Graduate School of Huazhong University of Science and Technology announced it would expel students who were taking too long to complete advanced degrees. The relevant regulations say completing a master’s degree shouldn’t take longer than four years and a doctor’s degree eight.
