Effects of auricular point pressing + Danshen Dropping Pill on platelet function, inflammatory response and protease expression in patients with stable angina pectoris

Objective:To investigate the effects of auricular point pressing plus Danshen Dropping Pill on platelet function, inflammatory response and protease expression in patients with stable angina pectoris.Methods:A total of 92 patients with stable angina pectoris who were treated in this hospital between November 2014 and November 2017 were divided into the auricular point pressing group (n=46) and routine group (n=46) by random number table method. Routine group were treated with routine western medicine plus Danshen Dropping Pill, auricular point pressing group were treated with auricular point pressing on the basis of the therapy of routine group, and both groups had been continuously treated for 1 month. The differences in platelet function, inflammatory response and protease expression were compared between the two groups before and after treatment.Results:Before treatment, there was no statistically significant difference in the platelet activation index levels, serum inflammatory factor contents or protease expression between the two groups. After 1 month of treatment, peripheral blood platelet activation index CD42b level of auricular point pressing group was higher than that of routine group whereas CD63 and PAC-1 levels were lower than those of routine group;serum inflammatory factors IL-6, IL-10, IL-18 and hs-CRP contents were lower than those of routine group;serum proteases MMP-1, MMP-2 and MMP-9 contents were lower than those of routine group.Conclusion:Compound Danshen Dropping Pill combined with auricular point pressing can further optimize the platelet function and improve the systemic inflammatory state and protease expression in patients with stable angina pectoris.

Expression of ceramide galactosy transferase in sciatic nerve of rats with diabetic peripheral neuropathy after electroacupuncture at Zusanli and Shenshu points

BACKGROUND： Ceramide galactosyltransferase （CGT） protein and mRNA expression defect can cause the abnormal morphology and slowing conduction velocity of peripheral nerve. Morphologic change and functional disorder of myelin sheath and axon appear when diabetic peripheral neuropathy （DPN） occurs. Whether electroacupuncture at Zusanfi（ST 36） and Shenshu（BL 32） points can enhance the expression of CGT protein and mRNA in the DPN tissue？ OBJECTIVE： To observe the effect of electroacupuncture at Zusanfi and Shenshu points on motor, sensory conduction velocity and CGT mRNA and its protein expression of sciatic nerve in rats with DPN. DESIGN： A randomized and controlled animal experiment SETTING ： Department of Neurology and Central Laboratory, Yueyang Hospital of Traditional Chinese ＆ Western Medicine, Shanghai University of Traditional Chinese Medicine. MATERIALS ： Totally 60 healthy male Wistar rats of clean grade, aged 4 month, with body mass of 200 to 220 g, were enrolled in this study. Streptozotocin （STZ, Sigma Company of USA, Batch No. S-0130）. METHODS： This study was carried out in the Animal Experimental Center and Central Laboratory, Yueyang Hospital of Traditional Chinese ＆ Western Medicine during February 2005 to March 2006. （1） Fifteen rats were randomly chosen,serving as normal group.AU the other rats were intraperitoneally injected once with STZ to develop experimental diabetic rat models. If fasting blood glucose was ≥ 15 mmol/L,sensory nerve and motor nerve conduction velocity of sciatic nerve was obviously slowed, tail-swaying temperature threshold was increased and myelinated nerve fiber of sciatic nerve changed, DPN models were successful. The successful model rats were randomly assigned into 3 groups： model group, control group（electroacupuncture at non-meridian-non-acupoint）and electroacupuncture group [electroacupuncture at Zusan/i and Shenshu points], with 15 rats each. The rats in the normal group and model group were untouched. In the electroacupuncture group （electroacupuncture at Zusanfi and Shenshu points）, Shenshu point （double） and Zusanfi point （double） were chosen referencing to The Atlas of the Rat＇s Acupoints. G6805- Ⅱ electric acupuncture apparatus was used, and current intensity was controlled at 20 min/time, once every other day, 12 times within 24 days. In the control group, the tip of rat-tail was stimulated, and the concrete procedures were the same as in the electroacupuncture at Zusanfi and Shenshu points. （2） Motor nerve conduction velocity and sensory nerve conduction velocity of rats were detected with neuroelectrophysiology detector in the end of the treatment, and the expressions of CGT protein and mRNA of sciatic nerve were detected with immunohistochemical method and fluorescent quantitative PCR technique. MAIN OUTCOME MEASURES： （1） Motor and sensory nerve conduction velocity. （2） The expression of CGT protein and its mRNA. RESULTS： All the 60 rats entered the stage of result analysis. （1） Comparison of motor and sensory nerve conduction velocity of rats after electroacupuncture： Motor nerve conduction velocity of rats in the model group[（31.37±3.69） m/s], control group [（32.74±5.42） m/s] and electroacupuncture group [（41.30 ±1.15） m/s] was significantly lower than that in the normal group [（41.30±1.15） m/s, P 〈 0.01]; The sensory nerve conduction velocity of rats in the model group[（18.17±9.54） m/s], control group [（21.39±5.61） m/s]and electroacupuncture group [（35.81 ±4.59） m/s] was significantly lower than that in the normal group [（46.38± 6.32） m/s,P 〈 0.01]; The motor and sensory nerve conduction velocity of electroacupuncture group was significantly higher than that in the model group [（38.04±2.01） m/s vs. （32.74±5.42） m/s,（35.81±4.59） m/s vs. （21.39±5.61） m/s,P 〈 0.01]. （2） Comparison of the expression of CGT protein of sciatic nerve of rats： The number of CGT positive cells of sciatic nerve in model group, control group or electroacupuncture group was significantly smaller than that in normal group [（9 770.33±1 461.73）, （10 588.13±1119.52）, （27 518.27± 9 078.29）, （37 769.67±4 021.81）/μm^2,P 〈 0.01]; The number of CGT positive cells of the sciatic nerve in the electroacupuncture group was significantly larger than that in the model group and control group （P 〈 0.01）. The number of CGT positive cells of sciatic nerve was close between control group and electroacupuncture group （P 〉 0.05）. （3） Comparison of CGT mRNA expression of sciatic nerve of rats： Ct value of CGT mRNA of sciatic nerve of rats in the model group,control group and electroacupuncture group was significantly higher than that in the normal group （13.75±2.60,14.81±2.80,11.67±1.75,9.30±0.98, P 〈 0.01 ）; Ct value of CGT mRNA of sciatic nerve of rats in the electroacupuncture group was significantly lower than that in the model group and control group （P 〈 0.01）, and that was close between electroacupuncture group and control group （P 〉 0.05）. CONCLUSION ： Electroacupuncture at Zusanfi and Shenshu points can increase motor and sensory nerve conduction velocity of rats with DPN. It might be associated with up-regulating the expression of CGT mRNA and its protein.

Charge Point推出模块化Express Plus EV快速充电平台:高达400kW

Charge Point公司推出Charge Point Express Plus，一种可以提供高达400kW的超快直流充电解决方案，它支持的充电电压范围从200V到1000V，包括今天的400V轿车和750V客车以及明天的800V轿车。

Application of TREM-1 in peripheral blood as a switching point for sequential ventilation of patients with chronic obstructive pulmonary disease complicated with respiratory failure

Objective:To investigate the application of peripheral blood triggering receptor expressed on myeloid cells-1(TREM-1)for sequential treatment switching points in patients with chronic obstructive pulmonary disease(COPD)complicated with respiratory failure.Methods:A total of 120 cases of COPD patients with respiratory failure from June 2017 to December 2018 were randomly divided into two groups:60 cases in the control group and 60 cases in the observation group.The control group received spontaneous breathing trials for 2 h to select the time for non-invasive positive pressure ventilation,while the observation group received peripheral blood TREM-1(≤90.0 pg/mL)to select the time for non-invasive positive pressure ventilation.The stress hormones,clinical pulmonary infection score and vital signs of two groups after 24 h of mechanical ventilation were detected.The treatment time and the adverse reactions of the two groups were recorded.Results:There was no significant difference in rennin,adrenaline,noradrenaline and angiotensin II between two groups(P>0.05).Compared with the control group,the clinical pulmonary infection score was decreased in the observation group(P<0.05).There was no significant difference in heart rate,respiratory rate,pH,partial pressure of carbon dioxide and partial pressure of oxygen between two groups(P>0.05).There was no significant difference in intensive care monitoring time between two groups(P>0.05).Compared with the control group,the observation group had no significant difference in invasive ventilation time and total mechanical ventilation.The time of hospitalization and hospitalization had significantly decreased(P<0.05).There was no significant difference in mortality,ventilator-associated pneumonia and re-intubation between two groups(P>0.05).Conclusion:TREM-1 can be used as a switching point during invasive-noninvasive sequential ventilation for COPD patients with respiratory failure,which can shorten the time of invasive ventilation,total mechanical ventilation and hospitalization.

Effect of the Flanking Sequence Architecture of Translation Initiation AUG Codon on Gene Expression Level in Rice

The relationship between the codon usage bias, gene expression level and the AUG context(from -20 to +6 positions relative to the initiator AUG codon) was examined in 541unigene sequences of rice. A significant correlation for CAI values (codon adaptationindex) was observed at five nucleotide positions (-19, -18, -9, -4, +5), eight (-19, -18,-14, -9, -6, -4, -1, +5) for CPP (codon preference parameter), and seven (-18, -16, -15,-9, -7, -1, +6) for mRNA abundance in the flanking sequence of the initiator AUG codonrespectively, but a significantly positive correlation for both CAI and CPP at twopositions (-4 and +5), indicating that both those positions are evolutionally under thenatural selection constraint at the translational level. By site-directed mutagenesis atseven specific positions (-18, -16, -15, -9, -7, -1 and +6) for allergenic protein thathad the highest mRNA abundance in this study, its expression level decreased dramatically63.3 and 72.5% respectively, indicating the importance of those 7 positions for geneexpression. A highly positive correlation (r=0.625, P<0.01) between AUGCAI and GCcontent in the flanking sequence of the initiator AUG codon showed a more effectivehigher GC content on translation initiation efficiency. The strong preference for G orC at those 8 positions (-6, -5, -3, -2, -1, +4, +5 and +6) in the AUG context suggestedthat an important factor in modulation of the translation efficiency, as well assynonymous codon usage bias, particularly in highly expressed genes.

Symbolic Expressions of Differences between Earth Radii

A systematic and comprehensive comparison of the five commonly used earth radii in geodesy and cartography is carried out,and the differences between the most common points of the earth radii,their corresponding maximum values,and the latitudes of equal points between them are derived with the help of computer algebraic systems.The symbolic expressions are expressed as a power series of the first eccentricity.Taking the CGCS2000 ellipsoid as an example,the differences between the commonly used earth radii are clarified to numerical values.The results show that the difference between the commonly used earth radii has a maximum at 90 degrees and a minimum at 0 degree.The difference between the average radius of curvature and the rectifying sphere radius is the biggest,and the difference between the average radius of curvature and the average sphere radius is the smallest.These results can provide a theoretical basis for corresponding research in the geosciences,space science,navigation and positioning.

Check Point推出增强版中型企业安全解决方案

Check Point软件技术有限公司宣布推出Check Point Express CI(这是其为中型企业设计的整合安全解决方案的增强版本，在原本的基础上新增了防病毒保护功能。Check Point Express CI拥有便于使用和管理的平台，它具备了中型企业需要的所有重要安全功能，包括防火墙、VPN、侵入防御和整合的防病毒保护等。

ChargePoint公司推出400kW超快速直流充电平台

北美电动汽车充电网络公司Charge Point推出新一代电动汽车超快速直流充电解决方案——Express Plus模块化平台。采用该平台的充电桩可以为电动汽车提供高达400k W的输出功率,从而使电动汽车实现更快速的充电。Express Plus模块化平台主要针对大型车辆研制,如电动公共汽车和电动卡车而开发,

倍数与百分数的正确使用与表达

在生活与工作中常用到倍数和百分数,然而在汉语与英语的相关表述中却很容易出错。针对这一问题,从倍数、百分数的概念入手,通过具体的实例说明二者的正确使用方法,介绍了常用的倍数增减、百分数、百分点的翻译方法。在表述倍数增加时,需注意“增加几倍”和“增加到几倍”的区别,前者强调净增量,后者强调增加后的结果。在表述倍数减少时,应使用分数。倍数增减的译法均有多种,在翻译时要注意汉语与英语表达思维方式的差异与不同。百分数与百分点既有联系又有区别。百分数是将两个指标进行相对程度对比(相除),而百分点是将两个指标进行绝对程度对比(相减)。用百分数翻译倍数减少时,需注意净减数和减少到某个数量的区别,正确使用介词by和to。

信息触达:灾害预警信息语言表达的影响研究

在自然灾害情境下,应急预警信息的有效触达对目标受众提前做好应急预案、减轻灾害损失具有重要价值,是影响应急管理效果的关键因素之一。本研究基于前景理论与参考点效应,运用实验研究方法收集城市暴雨灾害情境下预警信息受众的自反馈感知和行为数据,对不同参考点语境下灾害预警信息表达对信息触达效果的影响进行研究。结果表明,基于不同参考点设计的信息表达方式对灾害预警信息的触达效果有不同影响,其中具体信息参考点和社会比较参考点显著影响信息受众对灾害严重性和风险性的感知,而消极影响参考点则影响不显著。同时,认知负担会影响包含综合参考点以及与防范措施有关的具体信息参考点的信息触达效果。本研究推进了信息触达理论研究,对于改进政务信息表达、提升灾害预警信息触达效果、增强自然灾害应急管理能力具有重要意义。

基于规则判别的末端配送停留点识别与出行链特征

响应需求的末端配送方案可显著提升顾客满意度,识别并提取末端配送快递三轮车配送停留点特征是分析配送时空分布和动态需求的基础。因此,本文提出结合兴趣点(POI)与停留时长规则的停留点识别方法。首先,利用POI信息和瞬时速度初步筛选快递三轮车轨迹数据;然后,引入停留时长阈值作为二次筛选条件;最后,合并临近的聚集点,形成完整的停留点集。采用人工校验识别结果的准确性,并借助熵率法计算停留链的熵率,量化评估不同识别方法的精确度。以苏州市顺丰速运快递网点的快递三轮车配送轨迹数据为实证对象,将所提出的方法与货运卡车停留点识别中常用的基于密度的聚类算法(Density-Based Spatial Clustering of Applications with Noise,DBSCAN)进行对比分析。结果表明,DBSCAN算法易将交通信号灯等待误判为配送停留点,而本文所提出的方法则有效规避了该问题,实现高达98%的精确率和召回率;同时,熵率法的应用进一步验证了所提方法在准确率上的有效性。在此基础上,扩大研究范围并识别配送停留点后,分析快递三轮车的出行链与配送时空分布特征。结果表明,8:00左右的高峰期配送车辆数显著多于16:00左右的高峰期;住宅区为配送热点,车辆数最多,且出行距离和工作时长最长;酒店类配送呈现停留时长较短的特点;此外,停留点空间分布亦揭示了部分配送距离偏远的情况。

基于物联网的一种新型智能快递柜的设计

该文设计了一款安装在高层楼宇外的新型智能快递柜,该快递柜包含了若干个柜子用于存放快递,同时还开发了相应的微信小程序供快递员和用户使用。快递员将快递放置于系统自动分配的空闲柜子中,用户控制快递柜的升降绳索,将快递柜上升到自己所在楼层,从柜子中取出快递。该快递柜投入使用后能够改善物流末端的配送形式,实现物流最后一千米便捷、高效、安全的配送。

基于POI数据的南宁市快递自提点空间布局及影响因素分析

由于电子商务的快速发展和优异网购条件的刺激,网络购物得到了快速发展。如何建立高效的物流资源配置方式,提高末端配送服务时效性,解决物流实际“最后一公里”难题,成为减轻配送物流负担的关键。因此,基于南宁市下辖12个区县快递自提点POI数据,运用数理统计、空间地理分析等研究方法,对南宁市快递自提点空间基本特征和分布格局进行研究,结合经济因素、交通因素、人口分布因素等多重因素探索南宁市快递自提点布局模式并提出优化对策和建议。

Characterization of Fertility Alteration in 8 Environment-sensitive Genic Male-sterile Lines of indica Rice

Eight indica ( Oryza sativa L.) environment-sensitive genic male-sterile (EGMS) lines, 2-2S, K1405S, F131S, 2136S, Pei-Ai 64S, 1290S, GD-IS and N17S, were sequentially seeded with 10-15 d interval at three sites, Wuhan in 1997, Guiyang in 1997 and Sanya in 1997 and 1998, China. The results of investigations on self-sterilities showed that all of eight EGMS lines had stable sterile periods of longer than 30 d at Wuhan. They can be used for seed production of two-line hybrid rice, but can not reproduce themselves. Their stable sterile periods were shorter than 30 d at Guiyang, they can reproduce themselves and can not be used for hybrid seed production. In Sanya, their stable sterile periods were longer than 150 d, all of eight lines can be used for seed production in summer and autumn and reproduce themselves in winter. The fertility of all eight lines were sensitive to temperature. The sensitive stages, sensitive duration and critical point of temperatures (CPT) of fertility alteration in various lines were different. The sensitive stages of 2-2S and K1405S were from 18 d to 9 d before heading, the sensitive durations were 7-10 d and the CPTs were 23.7-24.5 degreesC. The sensitive stage, sensitive duration and CPT of F131S were from 17 to 5 d before heading, 13 d and 24.3-24.7 degreesC, respectively. The sensitive stage, sensitive duration and CPT of 2136S were from 18 to 12 d before heading, 7 d and 24.6-25.1 degreesC:, respectively. The sensitive stages, sensitive durations of Pei-Ai 64S, 1290S, N17S and GD-1S were from 24 to 13 d before heading and 10-13 d. And their CPTs were 24.6-25.1 degreesC, 25.5-26.2 degreesC, 25.4-26.1 degreesC,, and 24.1-24.7 degreesC, respectively.

“合谷”穴和口面部联系的解剖学基础

目的 :用形态学的方法探讨“合谷”穴与口面部的传入联系。方法 :本实验分神经生物素示踪和fos蛋白表达两部分 ,每部分均选用雌性Wistar大鼠 2 4只。将神经束路示踪剂神经生物素分别注射于“合谷”穴区和口面部 ,观察标记纤维在颈段脊髓和低位脑干的分布。电针“合谷”穴和口面部 ,利用免疫组织化学的方法 ,观察其二者的传入信息在颈髓和低位脑干诱导的fos样免疫反应阳性神经元的分布。结果 :“合谷”穴区的初级传入纤维主要止于颈髓 5～ 8节段 ;来自口面部的初级传入纤维主要止于同侧三叉神经脊束核 ,尚有少量分支直接投射至同侧孤束核和网状结构。电针“合谷”穴的传入信息除主要到达颈部脊髓背角外 ,也可到达孤束核和网状结构 ;而电针口面部的传入信息主要抵达同侧三叉神经脊束核、孤束核和网状结构 ,也可影响到颈髓背角等结构的神经元。结论 :“合谷”穴和口面部均与孤束核有着直接或间接的纤维联系 ,这可能是“合谷”穴和口面部联系的形态学基础。

电针涌泉穴对D-半乳糖致衰老模型大鼠的p53和bcl-2基因表达的影响

目的 研究针刺对衰老造模大鼠 p5 3、bcl- 2基因表达的影响。方法 用 D-半乳糖注射法建立大鼠衰老模型 ,经电针涌泉穴治疗后 ,用 RT- PCR方法检测 p5 3和 bcl- 2基因的表达情况。结果 电针能明显改善患鼠症状 ;D-半乳糖造模引起 p5 3基因表达上调 ,经电针涌泉穴后又可使其下调 ;bcl- 2基因的表达不受 D-半乳糖造模和电针的影响。结论 针刺涌泉穴对衰老大鼠的 p5

用基因芯片筛选针刺衰老大鼠涌泉穴的318个差异基因初报

目的 :应用基因芯片研究针刺涌泉穴对D 半乳糖所致衰老大鼠基因表达的影响。方法 :将成年健康大鼠随机分为模型组与针刺组 ,两组每日皮下注射 10 %D 半乳糖 0 5ml造模 ,造模的同时针刺组予以针刺涌泉穴 ,7周后处死 ,并摘取肝脏提取mRNA ,作逆转录为cDNA以备检测。随机选用 2 30 5条大鼠cDNA制备成表达谱芯片 ,用Cy3和Cy5两种荧光物质分别标记两组大鼠肝组织的cDNA ,制备成探针与表达谱芯片进行杂交 ,通过计算机扫描后进行数据分析 ,初步筛选出了差异表达基因 318条 ,其中表达上调的有 137条 ,表达下调的有 181条 ,并对其中差异最为显著的 5 5条作了初步分析。结果 :针刺涌泉穴对衰老大鼠的基因表达的影响涉及免疫、生长发育、基础代谢和细胞骨架运动等多个方面 ,这从基因水平证明了针刺治疗是一整体调节作用。结论 :应用基因芯片技术能够同时半定量的研究数千条基因的表达状态 ,可以从微观基因水平研究针刺的整体机理 ,并判定其分子疗效 。

昆虫乙酰胆碱酯酶基因变异抗药性机制研究

有机磷和氨基甲酸酯类杀虫剂的大量使用导致昆虫对其产生抗药性。乙酰胆碱酯酶是昆虫对这类杀虫剂产生抗性的重要的靶标酶,昆虫产生抗药性的重要原因之一,就是因为乙酰胆碱酯酶的基因表达量上升,或基因突变而导致其敏感性下降。文章简要论述昆虫乙酰胆碱酯酶基因发生变异而导致的抗药性,分析了变异对其结构和功能的影响。

针刺大鼠“相对穴”阳陵泉与阴陵泉对P物质表达的影响研究

目的:观察针刺大鼠相对穴阴陵泉与阳陵泉后穴区局部和血清中P物质(SP)的表达,探讨SP变化与"相对穴"协同增效的相关性。方法:除空白对照组外,其他各组大鼠给予电针干预后采用酶联免疫技术检测实验前后大鼠血清中SP含量,穴区局部组织组化染色观察穴区SP阳性表达。结果:与对照组比较,其他各针刺组穴区局部SP阳性表达和血清中含量均有不同程度的增多,其中针刺相对穴阳陵泉与阴陵泉穴组SP表达最为显著,各针刺组比较,针刺旁开阳陵泉与阴陵泉穴组SP表达显著低于其他各组。结论:针刺相对穴阳陵泉与阴陵泉穴SP穴区局部阳性表达和血清含量显著增高,是相对穴配伍协同增效的可能因素之一。

“十二井穴”针法对局灶性脑缺血大鼠TGF-β1的时效性影响

目的 :观察“十二井穴”针法对局灶性脑缺血大鼠TGF β1的时效性影响。方法 :采用光化学诱导Wistar大鼠一侧大脑造成局灶性脑缺血模型 ,施以“十二井穴”针法在造模后 1、3、6、1 2、2 4hr不同时间段开始进行针刺治疗 ,治疗 6天后处死大鼠 ,沿缺血中心制成冠状面切片 ,运用免疫组化染色观察各治疗组半暗带与对照组神经细胞TGF β1表达量的变化 ,并进行图像分析。结果 :各时段模型组TGF β1表达的总面积及 3、6、1 2、2 4hr各组的积分光度均比假手术及正常组明显升高 (P <0 0 5) ,针刺组 3、6、1 2、2 4hrTGF β1表达的总面积及 6、1 2、2 4hrTGF β1表达的积分光度均较同时段模型组明显升高 (P <0 0 5或P <0 0 1 )。结论