Riemann proved three results: analytically continue ζ(s) over the whole complex plane s =σ + it with a pole s =1;(Theorem A) functional equation ξ(t) = G(s<sub>0</sub>)ζ (s<sub>0</sub>), s&...Riemann proved three results: analytically continue ζ(s) over the whole complex plane s =σ + it with a pole s =1;(Theorem A) functional equation ξ(t) = G(s<sub>0</sub>)ζ (s<sub>0</sub>), s<sub>0</sub> =1/2 + it and (Theorem B) product expression ξ<sub>1</sub>(t) by all roots of ξ(t). He stated Riemann conjecture (RC): All roots of ξ (t) are real. We find a mistake of Riemann: he used the same notation ξ(t) in two theorems. Theorem B must contain complex roots;it conflicts with RC. Thus theorem B can only be used by contradiction. Our research can be completed on s<sub>0</sub> =1/2 + it. Using all real roots r<sub>k</sub><sub> </sub>and (true) complex roots z<sub>j</sub> = t<sub>j</sub> + ia<sub>j</sub> of ξ (z), define product expressions w(t), w(0) =ξ(0) and Q(t) > 0, Q(0) =1 respectively, so ξ<sub>1</sub>(t) = w(t)Q(t). Define infinite point-set L(ω) = {t : t ≥10 and |ζ(s<sub>0</sub>)| =ω} for small ω > 0. If ξ(t) has complex roots, then ω =ωQ(t) on L(ω). Finally in a large interval of the first module |z<sub>1</sub>|>>1, we can find many points t ∈ L(ω) to make Q(t) . This contraction proves RC. In addition, Riemann hypothesis (RH) ζ for also holds, but it cannot be proved by ζ.展开更多
Functional magnetic resonance imaging was used during emotion recognition to identify changes in functional brain activation in 21 first-episode, treatment-naive major depressive disorder patients before and after ant...Functional magnetic resonance imaging was used during emotion recognition to identify changes in functional brain activation in 21 first-episode, treatment-naive major depressive disorder patients before and after antidepressant treatment. Following escitalopram oxalate treatment, patients exhibited decreased activation in bilateral precentral gyrus, bilateral middle frontal gyrus, left middle temporal gyrus, bilateral postcentral gyrus, left cingulate and right parahippocampal gyrus, and increased activation in right superior frontal gyrus, bilateral superior parietal Iobule and left occipital gyrus during sad facial expression recognition. After antidepressant treatment, patients also exhibited decreased activation in the bilateral middle frontal gyrus, bilateral cingulate and right parahippocampal gyrus, and increased activation in the right inferior frontal gyrus, left fusiform gyrus and right precuneus during happy facial expression recognition. Our experimental findings indicate that the limbic-cortical network might be a key target region for antidepressant treatment in major depressive disorder.展开更多
The expression and function in growth and apoptosis of the renin-angiotensin system(RAS)was evaluated inhuman glioblastoma.Renin and angiotensinogen(AGT)mRNAs and proteins were found by in situ hybridisationand immuno...The expression and function in growth and apoptosis of the renin-angiotensin system(RAS)was evaluated inhuman glioblastoma.Renin and angiotensinogen(AGT)mRNAs and proteins were found by in situ hybridisationand immunohistochemistry in glioblastoma cells.Angiotensinogen was present in glioblastoma cystic fluids.Thus,human glioblastoma cells produce renin and AGT and secrete AGT.Human glioblastoma and glioblastoma cellsexpressed renin,AGT,renin receptor,AT(2)and/or AT(1)mRNAs and proteins determined by RT-PCR and/展开更多
Eukaryotic translation initiation factor 1A(eIF1A)functions as an important regulatory factor of protein synthesis and plays a crucial role in responses to abiotic stresses in plants.However,little is known about the ...Eukaryotic translation initiation factor 1A(eIF1A)functions as an important regulatory factor of protein synthesis and plays a crucial role in responses to abiotic stresses in plants.However,little is known about the eIF1A gene involved in fruit development and stress response of mango.In this study,the MieIF1A-b gene was isolated from Mangifera indica,and contains a 435-bp open reading frame,which encodes a putative protein of 144 amino acids(GenBank accession number:KP676599).The predicted MieIF1A-b protein had a molecular weight of 16.39 kDa with a pI of 4.6.Sequence homology analysis showed that MieIF1A-b shared high homology with Elaeis guineensis,Manihot esculenta,and Populus trichocarpa,with 96 and 95%identity,respectively.Quantitative reverse transcriptative PCR(qRT-PCR)analyses indicated that MieIF1A-b was expressed in all tested tissues,and had the highest expression level in fruit 80 d after flowering.The expression of MieIF1A-b was obviously regulated by NaCl and H2O2 treatments in leaves.Functional analysis indicated that the overexpression of MieIF1A-b in transgenic Arabidopsis thaliana enhanced the growth,phenotype and salinity tolerance compared with wild-type(WT)plants.The results indicated that MieIF1A-b may be correlated with the control of fruit development and salt adaptation,and it was a candidate gene for abiotic stress in mango.展开更多
Compactly supported radial basis function can enable the coefficient matrix of solving weigh linear system to have a sparse banded structure, thereby reducing the complexity of the algorithm. Firstly, based on the com...Compactly supported radial basis function can enable the coefficient matrix of solving weigh linear system to have a sparse banded structure, thereby reducing the complexity of the algorithm. Firstly, based on the compactly supported radial basis function, the paper makes the complex quadratic function (Multiquadric, MQ for short) to be transformed and proposes a class of compactly supported MQ function. Secondly, the paper describes a method that interpolates discrete motion capture data to solve the motion vectors of the interpolation points and they are used in facial expression reconstruction. Finally, according to this characteris- tic of the uneven distribution of the face markers, the markers are numbered and grouped in accordance with the density level, and then be interpolated in line with each group. The approach not only ensures the accuracy of the deformation of face local area and smoothness, but also reduces the time complexity of computing.展开更多
To better understand the effect of a new split variant of human asialoglycoprotein receptor (ASGPR H1b) on ASGPR ligands’ binding ability, we established a functional cell line which expresses ASGPR.The full lengths ...To better understand the effect of a new split variant of human asialoglycoprotein receptor (ASGPR H1b) on ASGPR ligands’ binding ability, we established a functional cell line which expresses ASGPR.The full lengths of ASGPRH1a and H2c fragments from human liver were amplified by reverse transcript PCR (RT-PCR) and inserted into eukaryotic expression vector pIRES2EFP, pCDNA3.1 (Zeo+) respectively.The recombinants were cotransfected into HeLa cells.After selection by using Neocin and Zeocin, a stably transfected cell line was established, which was designated 4-1-6.The transcription and expression of ASGPRH1a and H2c in 4-1-6 were confirmed by RT-PCR, Western blotting and immunofluorescence.The endocytosis function of the artificial "ASGPR" on the surface of 4-1-6 was tested by FACS.It was found that the cell line 4-1-6 could bind ASGPR natural ligand molecular asialo-orosomucoid (ASOR).After the eukaryotic plasmid H1b/pCDNA3.1 (neo) was transfected into cell line 4-1-6, H1b did not down-regulate the ligand binding ability of ASGPR.The eukaryotic expression plasmid H1b/pcDNA3.1 (neo) and H2c/pcDNA3.1 (neo) were co-transfected transiently into Hela cell.Neither single H1b nor H1b and H2c could bind ASOR.In conclusion, a functional cell line of human asialoglycoprotein receptor (ASGPR) which expresses both H1a and H2c stably was established.The new split variant H1b has no effect on ASGPR binding to ASOR.ASGPRH1b alone can’t bind to ASOR, it yet can’t form functional complex with ASGPRH2c.展开更多
Background The impairment of facial expression recognition has become a biomarker for early identification of first-episode schizophrenia, and this kind of research is increasing.Aims To explore the differences in bra...Background The impairment of facial expression recognition has become a biomarker for early identification of first-episode schizophrenia, and this kind of research is increasing.Aims To explore the differences in brain area activation using different degrees of disgusted facial expression recognition in antipsychotic-na?ve patients with firstepisode schizophrenia and healthy controls.Methods In this study, facial expression recognition tests were performed on 30 first-episode, antipsychoticna?ve patients with schizophrenia(treatment group) and 30 healthy subjects(control group) with matched age, educational attainment and gender. Functional MRI was used for comparing the differences of the brain areas of activation between the two groups.Results The average response time difference between the patient group and the control group in the ‘high degree of disgust' facial expression recognition task was statistically significant(1.359(0.408)/2.193(0.625), F=26.65, p<0.001), and the correct recognition rate of the treatment group was lower than that of the control group(41.05(22.25)/59.84(13.91, F=19.81, p<0.001). Compared with the control group, the left thalamus, right lingual gyrus and right middle temporal gyrus were negatively activated in the patients with first-episode schizophrenia in the ‘high degree of disgust' emotion recognition, and there was a significant activation in the left and right middle temporal gyrus and the right caudate nucleus. However, there was no significant activation difference in the ‘low degree of disgust' recognition.Conclusions In patients with first-episode schizophrenia, the areas of facial recognition impairment are significantly different in different degrees of disgust facial expression recognition.展开更多
Brain oscillations are vital to cognitive functions,while disrupted oscillatory activity is linked to various brain disorders.Although high-frequency neural oscillations(>1 Hz)have been extensively studied in cogni...Brain oscillations are vital to cognitive functions,while disrupted oscillatory activity is linked to various brain disorders.Although high-frequency neural oscillations(>1 Hz)have been extensively studied in cognition,the neural mechanisms underlying low-frequency hemodynamic oscillations(LFHO)<1 Hz have not yet been fully explored.One way to examine oscillatory neural dynamics is to use a facial expression(FE)paradigm to induce steady-state visual evoked potentials(SSVEPs),which has been used in electroencephalography studies of high-frequency brain oscillation activity.In this study,LFHO during SSVEP-inducing periodic flickering stimuli presentation were inspected using functional near-infrared spectroscopy(fNIRS),in which hemodynamic responses in the prefrontal cortex were recorded while participants were passively viewing dynamic FEs flickering at 0.2 Hz.The fast Fourier analysis results demonstrated that the power exhibited monochronic peaks at 0.2 Hz across all channels,indicating that the periodic events successfully elicited LFHO in the prefrontal cortex.More importantly,measurement of LFHO can effectively distinguish the brain activation difference between different cognitive conditions,with happy FE presentation showing greater LFHO power than neutral FE presentation.These results demonstrate that stimuli flashing at a given frequency can induce LFHO in the prefrontal cortex,which provides new insights into the cognitive mechanisms involved in slow oscillation.展开更多
In this paper, it is pointed that the general expression for the stress function of the plane problem in polar coordinates is incomplete. The problems of the curved bar with an arbitrary distributive load at the bound...In this paper, it is pointed that the general expression for the stress function of the plane problem in polar coordinates is incomplete. The problems of the curved bar with an arbitrary distributive load at the boundries can't he solved by this stress function. For this reason, we suggest two new stress functions and put them into the general expression. Then, the problems of the curved bar applied with an arbitrary distributive load at r=a,b boundaries can be solved. This is a new stress function including geometric boundary constants.展开更多
The opioid receptor-libel receptor (ORL), an orphan receptor whose human and murine complementary DNAs,has been characterized recently. ORL transcripts are particularly abundant in the central nervous system. We demon...The opioid receptor-libel receptor (ORL), an orphan receptor whose human and murine complementary DNAs,has been characterized recently. ORL transcripts are particularly abundant in the central nervous system. We demonstrated that ORL expressed in human neuroblastoma SK-N-SH and SH-SY5Y cell lines by radioligand binding assay, reverse transcription polymerase chain reaction (RT-PCR) and Northern analysis in the present study. Stimulation with ORL1 specific agonist, nociceptin/orphanin Fo, increased [34S]GTPrγS binding to SK-N-SH cell membranes (EC50 = 14 ±0.45 nM), and attenuated forskolin-stimulated accumulation of cellular cAMP (EC50= 0.80 ±0.45 nM, indicative that activation of ORL1 activates G proteins and inhibits adenylyl cyclase. Activation of ORL1 receptor was also accessed using CHO:hORL1 cell line by microphysiometer. Treatment of nociceptin/orphanin FQ increased extracellular acidification rate significantly.展开更多
Objective:To investigate the effects of auricular point pressing plus Danshen Dropping Pill on platelet function, inflammatory response and protease expression in patients with stable angina pectoris.Methods:A total o...Objective:To investigate the effects of auricular point pressing plus Danshen Dropping Pill on platelet function, inflammatory response and protease expression in patients with stable angina pectoris.Methods:A total of 92 patients with stable angina pectoris who were treated in this hospital between November 2014 and November 2017 were divided into the auricular point pressing group (n=46) and routine group (n=46) by random number table method. Routine group were treated with routine western medicine plus Danshen Dropping Pill, auricular point pressing group were treated with auricular point pressing on the basis of the therapy of routine group, and both groups had been continuously treated for 1 month. The differences in platelet function, inflammatory response and protease expression were compared between the two groups before and after treatment.Results:Before treatment, there was no statistically significant difference in the platelet activation index levels, serum inflammatory factor contents or protease expression between the two groups. After 1 month of treatment, peripheral blood platelet activation index CD42b level of auricular point pressing group was higher than that of routine group whereas CD63 and PAC-1 levels were lower than those of routine group;serum inflammatory factors IL-6, IL-10, IL-18 and hs-CRP contents were lower than those of routine group;serum proteases MMP-1, MMP-2 and MMP-9 contents were lower than those of routine group.Conclusion:Compound Danshen Dropping Pill combined with auricular point pressing can further optimize the platelet function and improve the systemic inflammatory state and protease expression in patients with stable angina pectoris.展开更多
Fiber cell initiation is a complex process involving many pathways,including phytohormones and components for transcriptional and posttranscriptional regulation.Here we report expression
The severe environmental problems and the demand for energy urgently require electrocatalysis to replace Haber-Bosch for the nitrogen reduction reaction(NRR).The descriptors and important properties of single-atom and...The severe environmental problems and the demand for energy urgently require electrocatalysis to replace Haber-Bosch for the nitrogen reduction reaction(NRR).The descriptors and important properties of single-atom and homonuclear double-atom catalysts have been preliminarily explored,but the relationship between the inherent properties and catalytic activity of heteronuclear double-atom catalysts with better performance remains unclear.Therefore,it is very significant to explore the prediction expressions of catalytic activity of heteronuclear double-atom catalysts based on their inherent properties and find the rule for selecting catalytic centers.Herein,by summarizing the free energy for the key steps of NRR on 55 catalysts calculated through the first-principle,the expressions of predicting the free energy and the corresponding descriptors are deduced by the machine learning,and the strategy for selecting the appropriate catalytic center is proposed.The selection strategy for the central atom of heteronuclear double-atom catalysts is that the atomic number of central B atom should be between group VB and VIIIB,and the electron difference between central A atom and B atom should be large enough,and the selectivity of NRR or hydrogen evolution reaction(HER)could be calculated through the prediction formula.Moreover,five catalysts are screened to have low limiting potential and excellent selectivity,and are further analyzed by electron transfer.This work explores the relationship between the inherent properties of heteronuclear double-atom catalysts and the catalytic activity,and puts forward the rules for selecting the heteronuclear double-atom catalytic center,which has guiding significance for the experiment.展开更多
Expression of opioid receptor-like receptor (ORL1)and its endogenous peptide agonist nociceptin/orphaninFo (N/OFQ) during mouse embryogenesis have been investigated. Transcripts of ORL1 and N/OFQ were detected by RT-P...Expression of opioid receptor-like receptor (ORL1)and its endogenous peptide agonist nociceptin/orphaninFo (N/OFQ) during mouse embryogenesis have been investigated. Transcripts of ORL1 and N/OFQ were detected by RT-PCR in mouse brain of day 8 embryo (E8)and the expression continued afterwards. Northern blotanalysis revealed abundant expression of ORL1 at postnatal day 1 (P1) and N/OFQ at E17 and P1 in the brain butnone was detected in other embryonic tissues. The presence of functional ORL1 in mouse embryonic brain wasalso confirmed by specific binding of [3H] N/OFQ (kd=1.3±0.5 nM and Bmax = 72±9 fmol/mg protein) as wellas by N/OFQ-stimulated G protein activation.展开更多
Mycoplasma genitalium is the main causative agent for non-gonococcal and non-chlamydial urethritis. P32 is the putative surface-exposed membrane protein of M. genitalium and it has substaintial identity in amino acid ...Mycoplasma genitalium is the main causative agent for non-gonococcal and non-chlamydial urethritis. P32 is the putative surface-exposed membrane protein of M. genitalium and it has substaintial identity in amino acid sequence with adhesin protein P30 from M. pnewnoniae. Since M. pneumoniae mutants lacking P30 protein is defective in cytadherence, P32 protein has been proposed to be an essential adhesin implicated in the adherence of M. genitalium to host cells. The prokaryotic expression vector pET-30 ( + )/p32 was constructed in the present study, and the recombinant protein was expressed in E. coli and purified under denaturing condition. As demonstrated by the immunoblotting analysis, the recombinant protein could react with rabbit antisera against M. genitalium, and adherence inhibition assays were petformed with antisera against this recombinant protein. It was demonstrated that P32 protein apperared to be an adhesion protein of M. genitalium, thus providing the experimental basis for better understanding of the pathogenesis of M. genitalium infection and for the development of the related vaccines against the infection.展开更多
文摘Riemann proved three results: analytically continue ζ(s) over the whole complex plane s =σ + it with a pole s =1;(Theorem A) functional equation ξ(t) = G(s<sub>0</sub>)ζ (s<sub>0</sub>), s<sub>0</sub> =1/2 + it and (Theorem B) product expression ξ<sub>1</sub>(t) by all roots of ξ(t). He stated Riemann conjecture (RC): All roots of ξ (t) are real. We find a mistake of Riemann: he used the same notation ξ(t) in two theorems. Theorem B must contain complex roots;it conflicts with RC. Thus theorem B can only be used by contradiction. Our research can be completed on s<sub>0</sub> =1/2 + it. Using all real roots r<sub>k</sub><sub> </sub>and (true) complex roots z<sub>j</sub> = t<sub>j</sub> + ia<sub>j</sub> of ξ (z), define product expressions w(t), w(0) =ξ(0) and Q(t) > 0, Q(0) =1 respectively, so ξ<sub>1</sub>(t) = w(t)Q(t). Define infinite point-set L(ω) = {t : t ≥10 and |ζ(s<sub>0</sub>)| =ω} for small ω > 0. If ξ(t) has complex roots, then ω =ωQ(t) on L(ω). Finally in a large interval of the first module |z<sub>1</sub>|>>1, we can find many points t ∈ L(ω) to make Q(t) . This contraction proves RC. In addition, Riemann hypothesis (RH) ζ for also holds, but it cannot be proved by ζ.
基金supported by research grants from the National Natural Science Foundation of China (No. 81071099)the Liaoning Science and Technology Foundation (No. 2008225010-14)Doctoral Foundation of the First Affiliated Hospital in China Medical University (No. 2010)
文摘Functional magnetic resonance imaging was used during emotion recognition to identify changes in functional brain activation in 21 first-episode, treatment-naive major depressive disorder patients before and after antidepressant treatment. Following escitalopram oxalate treatment, patients exhibited decreased activation in bilateral precentral gyrus, bilateral middle frontal gyrus, left middle temporal gyrus, bilateral postcentral gyrus, left cingulate and right parahippocampal gyrus, and increased activation in right superior frontal gyrus, bilateral superior parietal Iobule and left occipital gyrus during sad facial expression recognition. After antidepressant treatment, patients also exhibited decreased activation in the bilateral middle frontal gyrus, bilateral cingulate and right parahippocampal gyrus, and increased activation in the right inferior frontal gyrus, left fusiform gyrus and right precuneus during happy facial expression recognition. Our experimental findings indicate that the limbic-cortical network might be a key target region for antidepressant treatment in major depressive disorder.
文摘The expression and function in growth and apoptosis of the renin-angiotensin system(RAS)was evaluated inhuman glioblastoma.Renin and angiotensinogen(AGT)mRNAs and proteins were found by in situ hybridisationand immunohistochemistry in glioblastoma cells.Angiotensinogen was present in glioblastoma cystic fluids.Thus,human glioblastoma cells produce renin and AGT and secrete AGT.Human glioblastoma and glioblastoma cellsexpressed renin,AGT,renin receptor,AT(2)and/or AT(1)mRNAs and proteins determined by RT-PCR and/
基金supported by the National Natural Science Foundation of China (31660561)the Natural Science Foundation of Guangxi, China (2015GXNSFAA139052)+3 种基金the Key Research and Development Project of Guangxi, China (GXKJ-AB17292010)the Major Science and Technology Projects of Guangxi, China (GXKJ-AA17204097-3 and GXKJ-AA172040 26-2)the Innovation Team of Guangxi Mango Industry Project, China (nycytxgxcxtd-06-02)the State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, China (SKLCUSA-a201906)
文摘Eukaryotic translation initiation factor 1A(eIF1A)functions as an important regulatory factor of protein synthesis and plays a crucial role in responses to abiotic stresses in plants.However,little is known about the eIF1A gene involved in fruit development and stress response of mango.In this study,the MieIF1A-b gene was isolated from Mangifera indica,and contains a 435-bp open reading frame,which encodes a putative protein of 144 amino acids(GenBank accession number:KP676599).The predicted MieIF1A-b protein had a molecular weight of 16.39 kDa with a pI of 4.6.Sequence homology analysis showed that MieIF1A-b shared high homology with Elaeis guineensis,Manihot esculenta,and Populus trichocarpa,with 96 and 95%identity,respectively.Quantitative reverse transcriptative PCR(qRT-PCR)analyses indicated that MieIF1A-b was expressed in all tested tissues,and had the highest expression level in fruit 80 d after flowering.The expression of MieIF1A-b was obviously regulated by NaCl and H2O2 treatments in leaves.Functional analysis indicated that the overexpression of MieIF1A-b in transgenic Arabidopsis thaliana enhanced the growth,phenotype and salinity tolerance compared with wild-type(WT)plants.The results indicated that MieIF1A-b may be correlated with the control of fruit development and salt adaptation,and it was a candidate gene for abiotic stress in mango.
基金Supported by the National Natural Science Foundation of China (No.60875046)by Program for Changjiang Scholars and Innovative Research Team in University(No.IRT1109)+5 种基金the Key Project of Chinese Ministry of Education (No.209029)the Program for Liaoning Excellent Talents in University(No.LR201003)the Program for Liaoning Science and Technology Research in University (No.LS2010008,2009S008,2009S009,LS2010179)the Program for Liaoning Innovative Research Team in University(Nos.2009T005,LT2010005,LT2011018)Natural Science Foundation of Liaoning Province (201102008)by "Liaoning BaiQianWan Talents Program(2010921010,2011921009)"
文摘Compactly supported radial basis function can enable the coefficient matrix of solving weigh linear system to have a sparse banded structure, thereby reducing the complexity of the algorithm. Firstly, based on the compactly supported radial basis function, the paper makes the complex quadratic function (Multiquadric, MQ for short) to be transformed and proposes a class of compactly supported MQ function. Secondly, the paper describes a method that interpolates discrete motion capture data to solve the motion vectors of the interpolation points and they are used in facial expression reconstruction. Finally, according to this characteris- tic of the uneven distribution of the face markers, the markers are numbered and grouped in accordance with the density level, and then be interpolated in line with each group. The approach not only ensures the accuracy of the deformation of face local area and smoothness, but also reduces the time complexity of computing.
基金supported by grants from the National Major Science and Technology Special Project for Infectious Diseases of China (No.2008ZX10002-011)National High Technology Research and Development of China (Program 863) (No.2006AA02Z128)the National Natural Science Foundation of China (Nos.30700701,30571646)
文摘To better understand the effect of a new split variant of human asialoglycoprotein receptor (ASGPR H1b) on ASGPR ligands’ binding ability, we established a functional cell line which expresses ASGPR.The full lengths of ASGPRH1a and H2c fragments from human liver were amplified by reverse transcript PCR (RT-PCR) and inserted into eukaryotic expression vector pIRES2EFP, pCDNA3.1 (Zeo+) respectively.The recombinants were cotransfected into HeLa cells.After selection by using Neocin and Zeocin, a stably transfected cell line was established, which was designated 4-1-6.The transcription and expression of ASGPRH1a and H2c in 4-1-6 were confirmed by RT-PCR, Western blotting and immunofluorescence.The endocytosis function of the artificial "ASGPR" on the surface of 4-1-6 was tested by FACS.It was found that the cell line 4-1-6 could bind ASGPR natural ligand molecular asialo-orosomucoid (ASOR).After the eukaryotic plasmid H1b/pCDNA3.1 (neo) was transfected into cell line 4-1-6, H1b did not down-regulate the ligand binding ability of ASGPR.The eukaryotic expression plasmid H1b/pcDNA3.1 (neo) and H2c/pcDNA3.1 (neo) were co-transfected transiently into Hela cell.Neither single H1b nor H1b and H2c could bind ASOR.In conclusion, a functional cell line of human asialoglycoprotein receptor (ASGPR) which expresses both H1a and H2c stably was established.The new split variant H1b has no effect on ASGPR binding to ASOR.ASGPRH1b alone can’t bind to ASOR, it yet can’t form functional complex with ASGPRH2c.
基金Shanghai Mental Health Center hospital-level research project(2016-YJ-04)National Key Technology R&D Program of China during the 10th Five-Year Plan Period(2007BAI17B04)+2 种基金National Key Research and Development Program(2016YFC1306805)National Natural Science Foundation of China(81471359)Shanghai Municipal Committee of Science and Technology Guide Project of Chinese and Western Medicine(14411963400)
文摘Background The impairment of facial expression recognition has become a biomarker for early identification of first-episode schizophrenia, and this kind of research is increasing.Aims To explore the differences in brain area activation using different degrees of disgusted facial expression recognition in antipsychotic-na?ve patients with firstepisode schizophrenia and healthy controls.Methods In this study, facial expression recognition tests were performed on 30 first-episode, antipsychoticna?ve patients with schizophrenia(treatment group) and 30 healthy subjects(control group) with matched age, educational attainment and gender. Functional MRI was used for comparing the differences of the brain areas of activation between the two groups.Results The average response time difference between the patient group and the control group in the ‘high degree of disgust' facial expression recognition task was statistically significant(1.359(0.408)/2.193(0.625), F=26.65, p<0.001), and the correct recognition rate of the treatment group was lower than that of the control group(41.05(22.25)/59.84(13.91, F=19.81, p<0.001). Compared with the control group, the left thalamus, right lingual gyrus and right middle temporal gyrus were negatively activated in the patients with first-episode schizophrenia in the ‘high degree of disgust' emotion recognition, and there was a significant activation in the left and right middle temporal gyrus and the right caudate nucleus. However, there was no significant activation difference in the ‘low degree of disgust' recognition.Conclusions In patients with first-episode schizophrenia, the areas of facial recognition impairment are significantly different in different degrees of disgust facial expression recognition.
基金University of Macao,Nos.MYRG2019-00082-FHS and MYRG2018-00081-FHSMacao Science and Technology Development Fund,No.FDCT 025/2015/A1 and FDCT 0011/2018/A1.
文摘Brain oscillations are vital to cognitive functions,while disrupted oscillatory activity is linked to various brain disorders.Although high-frequency neural oscillations(>1 Hz)have been extensively studied in cognition,the neural mechanisms underlying low-frequency hemodynamic oscillations(LFHO)<1 Hz have not yet been fully explored.One way to examine oscillatory neural dynamics is to use a facial expression(FE)paradigm to induce steady-state visual evoked potentials(SSVEPs),which has been used in electroencephalography studies of high-frequency brain oscillation activity.In this study,LFHO during SSVEP-inducing periodic flickering stimuli presentation were inspected using functional near-infrared spectroscopy(fNIRS),in which hemodynamic responses in the prefrontal cortex were recorded while participants were passively viewing dynamic FEs flickering at 0.2 Hz.The fast Fourier analysis results demonstrated that the power exhibited monochronic peaks at 0.2 Hz across all channels,indicating that the periodic events successfully elicited LFHO in the prefrontal cortex.More importantly,measurement of LFHO can effectively distinguish the brain activation difference between different cognitive conditions,with happy FE presentation showing greater LFHO power than neutral FE presentation.These results demonstrate that stimuli flashing at a given frequency can induce LFHO in the prefrontal cortex,which provides new insights into the cognitive mechanisms involved in slow oscillation.
文摘In this paper, it is pointed that the general expression for the stress function of the plane problem in polar coordinates is incomplete. The problems of the curved bar with an arbitrary distributive load at the boundries can't he solved by this stress function. For this reason, we suggest two new stress functions and put them into the general expression. Then, the problems of the curved bar applied with an arbitrary distributive load at r=a,b boundaries can be solved. This is a new stress function including geometric boundary constants.
文摘The opioid receptor-libel receptor (ORL), an orphan receptor whose human and murine complementary DNAs,has been characterized recently. ORL transcripts are particularly abundant in the central nervous system. We demonstrated that ORL expressed in human neuroblastoma SK-N-SH and SH-SY5Y cell lines by radioligand binding assay, reverse transcription polymerase chain reaction (RT-PCR) and Northern analysis in the present study. Stimulation with ORL1 specific agonist, nociceptin/orphanin Fo, increased [34S]GTPrγS binding to SK-N-SH cell membranes (EC50 = 14 ±0.45 nM), and attenuated forskolin-stimulated accumulation of cellular cAMP (EC50= 0.80 ±0.45 nM, indicative that activation of ORL1 activates G proteins and inhibits adenylyl cyclase. Activation of ORL1 receptor was also accessed using CHO:hORL1 cell line by microphysiometer. Treatment of nociceptin/orphanin FQ increased extracellular acidification rate significantly.
文摘Objective:To investigate the effects of auricular point pressing plus Danshen Dropping Pill on platelet function, inflammatory response and protease expression in patients with stable angina pectoris.Methods:A total of 92 patients with stable angina pectoris who were treated in this hospital between November 2014 and November 2017 were divided into the auricular point pressing group (n=46) and routine group (n=46) by random number table method. Routine group were treated with routine western medicine plus Danshen Dropping Pill, auricular point pressing group were treated with auricular point pressing on the basis of the therapy of routine group, and both groups had been continuously treated for 1 month. The differences in platelet function, inflammatory response and protease expression were compared between the two groups before and after treatment.Results:Before treatment, there was no statistically significant difference in the platelet activation index levels, serum inflammatory factor contents or protease expression between the two groups. After 1 month of treatment, peripheral blood platelet activation index CD42b level of auricular point pressing group was higher than that of routine group whereas CD63 and PAC-1 levels were lower than those of routine group;serum inflammatory factors IL-6, IL-10, IL-18 and hs-CRP contents were lower than those of routine group;serum proteases MMP-1, MMP-2 and MMP-9 contents were lower than those of routine group.Conclusion:Compound Danshen Dropping Pill combined with auricular point pressing can further optimize the platelet function and improve the systemic inflammatory state and protease expression in patients with stable angina pectoris.
文摘Fiber cell initiation is a complex process involving many pathways,including phytohormones and components for transcriptional and posttranscriptional regulation.Here we report expression
基金supports by the National Natural Science Foundation of China(NSFC,52271113)the Natural Science Foundation of Shaanxi Province,China(2020JM 218)+1 种基金the Fundamental Research Funds for the Central Universities(CHD300102311405)HPC platform,Xi’an Jiaotong University。
文摘The severe environmental problems and the demand for energy urgently require electrocatalysis to replace Haber-Bosch for the nitrogen reduction reaction(NRR).The descriptors and important properties of single-atom and homonuclear double-atom catalysts have been preliminarily explored,but the relationship between the inherent properties and catalytic activity of heteronuclear double-atom catalysts with better performance remains unclear.Therefore,it is very significant to explore the prediction expressions of catalytic activity of heteronuclear double-atom catalysts based on their inherent properties and find the rule for selecting catalytic centers.Herein,by summarizing the free energy for the key steps of NRR on 55 catalysts calculated through the first-principle,the expressions of predicting the free energy and the corresponding descriptors are deduced by the machine learning,and the strategy for selecting the appropriate catalytic center is proposed.The selection strategy for the central atom of heteronuclear double-atom catalysts is that the atomic number of central B atom should be between group VB and VIIIB,and the electron difference between central A atom and B atom should be large enough,and the selectivity of NRR or hydrogen evolution reaction(HER)could be calculated through the prediction formula.Moreover,five catalysts are screened to have low limiting potential and excellent selectivity,and are further analyzed by electron transfer.This work explores the relationship between the inherent properties of heteronuclear double-atom catalysts and the catalytic activity,and puts forward the rules for selecting the heteronuclear double-atom catalytic center,which has guiding significance for the experiment.
文摘Expression of opioid receptor-like receptor (ORL1)and its endogenous peptide agonist nociceptin/orphaninFo (N/OFQ) during mouse embryogenesis have been investigated. Transcripts of ORL1 and N/OFQ were detected by RT-PCR in mouse brain of day 8 embryo (E8)and the expression continued afterwards. Northern blotanalysis revealed abundant expression of ORL1 at postnatal day 1 (P1) and N/OFQ at E17 and P1 in the brain butnone was detected in other embryonic tissues. The presence of functional ORL1 in mouse embryonic brain wasalso confirmed by specific binding of [3H] N/OFQ (kd=1.3±0.5 nM and Bmax = 72±9 fmol/mg protein) as wellas by N/OFQ-stimulated G protein activation.
基金National Natural Science Foundation of China(No.30570093).
文摘Mycoplasma genitalium is the main causative agent for non-gonococcal and non-chlamydial urethritis. P32 is the putative surface-exposed membrane protein of M. genitalium and it has substaintial identity in amino acid sequence with adhesin protein P30 from M. pnewnoniae. Since M. pneumoniae mutants lacking P30 protein is defective in cytadherence, P32 protein has been proposed to be an essential adhesin implicated in the adherence of M. genitalium to host cells. The prokaryotic expression vector pET-30 ( + )/p32 was constructed in the present study, and the recombinant protein was expressed in E. coli and purified under denaturing condition. As demonstrated by the immunoblotting analysis, the recombinant protein could react with rabbit antisera against M. genitalium, and adherence inhibition assays were petformed with antisera against this recombinant protein. It was demonstrated that P32 protein apperared to be an adhesion protein of M. genitalium, thus providing the experimental basis for better understanding of the pathogenesis of M. genitalium infection and for the development of the related vaccines against the infection.