期刊文献+
共找到690篇文章
< 1 2 35 >
每页显示 20 50 100
WSN Mobile Target Tracking Based on Improved Snake-Extended Kalman Filtering Algorithm
1
作者 Duo Peng Kun Xie Mingshuo Liu 《Journal of Beijing Institute of Technology》 EI CAS 2024年第1期28-40,共13页
A wireless sensor network mobile target tracking algorithm(ISO-EKF)based on improved snake optimization algorithm(ISO)is proposed to address the difficulty of estimating initial values when using extended Kalman filte... A wireless sensor network mobile target tracking algorithm(ISO-EKF)based on improved snake optimization algorithm(ISO)is proposed to address the difficulty of estimating initial values when using extended Kalman filtering to solve the state of nonlinear mobile target tracking.First,the steps of extended Kalman filtering(EKF)are introduced.Second,the ISO is used to adjust the parameters of the EKF in real time to adapt to the current motion state of the mobile target.Finally,the effectiveness of the algorithm is demonstrated through filtering and tracking using the constant velocity circular motion model(CM).Under the specified conditions,the position and velocity mean square error curves are compared among the snake optimizer(SO)-EKF algorithm,EKF algorithm,and the proposed algorithm.The comparison shows that the proposed algorithm reduces the root mean square error of position by 52%and 41%compared to the SOEKF algorithm and EKF algorithm,respectively. 展开更多
关键词 wireless sensor network(WSN)target tracking snake optimization algorithm extended Kalman filter maneuvering target
下载PDF
Labeled box-particle CPHD filter for multiple extended targets tracking 被引量:4
2
作者 ZOU Zhibin SONG Liping CHENG Xuan 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2019年第1期57-67,共11页
In multiple extended targets tracking, replacing traditional multiple measurements with a rectangular region of the nonzero volume in the state space inspired by the box-particle idea is exactly suitable to deal with ... In multiple extended targets tracking, replacing traditional multiple measurements with a rectangular region of the nonzero volume in the state space inspired by the box-particle idea is exactly suitable to deal with extended targets, without distinguishing the measurements originating from the true targets or clutter.Based on our recent work on extended box-particle probability hypothesis density(ET-BP-PHD) filter, we propose the extended labeled box-particle cardinalized probability hypothesis density(ET-LBP-CPHD) filter, which relaxes the Poisson assumptions of the extended target probability hypothesis density(PHD) filter in target numbers, and propagates not only the intensity function but also cardinality distribution. Moreover, it provides the identity of individual target by adding labels to box-particles. The proposed filter can improve the precision of estimating target number meanwhile achieve targets' tracks. The effectiveness and reliability of the proposed algorithm are verified by the simulation results. 展开更多
关键词 extended target MULTIPLE targetS tracking labled boxparticle cardinalized probability HYPOTHESIS density (CPHD).
下载PDF
Fast density peak-based clustering algorithm for multiple extended target tracking 被引量:4
3
作者 SHEN Xinglin SONG Zhiyong +1 位作者 FAN Hongqi FU Qiang 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2019年第3期435-447,共13页
The key challenge of the extended target probability hypothesis density (ET-PHD) filter is to reduce the computational complexity by using a subset to approximate the full set of partitions. In this paper, the influen... The key challenge of the extended target probability hypothesis density (ET-PHD) filter is to reduce the computational complexity by using a subset to approximate the full set of partitions. In this paper, the influence for the tracking results of different partitions is analyzed, and the form of the most informative partition is obtained. Then, a fast density peak-based clustering (FDPC) partitioning algorithm is applied to the measurement set partitioning. Since only one partition of the measurement set is used, the ET-PHD filter based on FDPC partitioning has lower computational complexity than the other ET-PHD filters. As FDPC partitioning is able to remove the spatially close clutter-generated measurements, the ET-PHD filter based on FDPC partitioning has good tracking performance in the scenario with more clutter-generated measurements. The simulation results show that the proposed algorithm can get the most informative partition and obviously reduce computational burden without losing tracking performance. As the number of clutter-generated measurements increased, the ET-PHD filter based on FDPC partitioning has better tracking performance than other ET-PHD filters. The FDPC algorithm will play an important role in the engineering realization of the multiple extended target tracking filter. 展开更多
关键词 FAST DENSITY peak-based clustering (FDPC) MULTIPLE extended target partition probability hypothesis DENSITY (PHD) filter track.
下载PDF
Extended differential geometric guidance law for intercepting maneuvering targets 被引量:4
4
作者 HUANG Jingshuai ZHANG Hongbo +1 位作者 TANG Guojian BAO Weimin 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2018年第5期1046-1057,共12页
Without assumptions made on motion states of missile and target, an extended differential geometric guidance law is derived. Through introducing a line of sight rotation coordinate system, the derivation is simplified... Without assumptions made on motion states of missile and target, an extended differential geometric guidance law is derived. Through introducing a line of sight rotation coordinate system, the derivation is simplified and has more explicit physical significances. The extended law is theoretically applicable to any engagement scenarios. Then, on basis of the extended law, a modified one is designed without the requirement of target acceleration and an approach is proposed to determining the applied direction of commanded missile acceleration. Qualitative analysis is carried out to study the capture performance and a criterion for capture is given. Simulation results indicate the two laws are effective and make up the deficiency that pure proportional navigation suitable for endoatmospheric interceptions cannot deal with high-speed maneuvering targets. Furthermore, the correctness of the criterion is validated. 展开更多
关键词 missile guidance maneuvering target extended differential geometric guidance law capture performance proportional navigation
下载PDF
Waveform design for radar and extended target in the environment of electronic warfare 被引量:3
5
作者 WANG Yuxi HUANG Guoce LI Wei 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2018年第1期48-57,共10页
Transmit waveform optimization is critical to radar system performance. There have been a fruit of achievements about waveform design in recent years. However, most of the existing methods are based on the assumption ... Transmit waveform optimization is critical to radar system performance. There have been a fruit of achievements about waveform design in recent years. However, most of the existing methods are based on the assumption that radar is smart and the target is dumb, which is not always reasonable in the modern electronic warfare. This paper focuses on the waveform design for radar and the extended target in the environment of electronic warfare. Three different countermeasure models between smart radar and dumb target, smart target and dumb radar, smart radar and smart target are proposed. Taking the signal-to-interferenceplus-noise ratio(SINR) as the metric, optimized waveforms for the first two scenarios are achieved by the general water-filling method in the presence of clutter. For the last case, the equilibrium between smart radar and smart target in the presence of clutter is given mathematically and the optimized solution is achieved through a novel two-step water-filling method on the basis of minmax theory. Simulation results under different power constraints show the power allocation strategies of radar and target and the output SINRs are analyzed. 展开更多
关键词 waveform design extended target electronic warfare CLUTTER water-filling method
下载PDF
Multiple extended target tracking algorithm based on Gaussian surface matrix 被引量:2
6
作者 Jinlong Yang Peng Li +1 位作者 Zhihua Li Le Yang 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2016年第2期279-289,共11页
In this paper, we consider the problem of irregular shapes tracking for multiple extended targets by introducing the Gaussian surface matrix(GSM) into the framework of the random finite set(RFS) theory. The Gaussi... In this paper, we consider the problem of irregular shapes tracking for multiple extended targets by introducing the Gaussian surface matrix(GSM) into the framework of the random finite set(RFS) theory. The Gaussian surface function is constructed first by the measurements, and it is used to define the GSM via a mapping function. We then integrate the GSM with the probability hypothesis density(PHD) filter, the Bayesian recursion formulas of GSM-PHD are derived and the Gaussian mixture implementation is employed to obtain the closed-form solutions. Moreover, the estimated shapes are designed to guide the measurement set sub-partition, which can cope with the problem of the spatially close target tracking. Simulation results show that the proposed algorithm can effectively estimate irregular target shapes and exhibit good robustness in cross extended target tracking. 展开更多
关键词 multiple extended target tracking irregular shape Gaussian surface matrix(GSM) probability hypothesis density(PHD)
下载PDF
Maneuvering Target Tracking Algorithm Based on Muti-paramter Sequential Extended Kalman Filter 被引量:2
7
作者 JIA Shuyi SUN Weiwei WANG Guohong 《Journal of Donghua University(English Edition)》 EI CAS 2018年第3期207-214,共8页
Based on the information theory,the performance of maneuvering target tracking can be improved by increasing the input information( observation vector).In this paper,the estimations of radial acceleration and radial v... Based on the information theory,the performance of maneuvering target tracking can be improved by increasing the input information( observation vector).In this paper,the estimations of radial acceleration and radial velocity obtained in the signal processing are introduced into the measurement vector by coordinate transformation.In order to solve the problem of high nonlinearity of the radial acceleration,radial velocity and the state vector,a new algorithm of multi-parameter sequential extended Kalman filter( MSEKF) is proposed.The tracking performance of this algorithm is tested and compared with the other tracking algorithms.It is shown that the proposed algorithm outperforms these algorithms in strong and weak maneuvering environments. 展开更多
关键词 information theory maneuvering target extended Kalman filter(EKF) radial acceleration radial velocity
下载PDF
Adaptive Extended Kalman Filtering for Bearings-Only Targets Tracking Problem
8
作者 胡恒章 周荻 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 1998年第3期1-4,共4页
An adaptive extended Kalman filtering (AEKF) is proposed for nonlinear control systems. For bearingsonly targets tracking problem, we present an adaptive extended Kalman filter which suits a nonlinear observation mode... An adaptive extended Kalman filtering (AEKF) is proposed for nonlinear control systems. For bearingsonly targets tracking problem, we present an adaptive extended Kalman filter which suits a nonlinear observation model and a linear dynamical model. Simulation results have shown that the adaptive extended Kalman filter for the passivetracking problem performs better than the original extended Kalman filter (EKF). 展开更多
关键词 extended KALMAN FILTERING adaptive extended KALMAN FILTERING BEARINGS-ONLY targetS TRACKING
下载PDF
MULTISENSOR DISTRIBUTED EXTENDED KALMAN FILTERING ALGORITHM AND ITS APPLICATION TO RADAR/IR TARGET TRACKING
9
作者 Cui Ningzhou Xie Weixin Yu Xiongnan Ma Yuanliang(Marine Engineering College, Northwestern Polytechnical University, Xi’an 710072) (Electronic Engineering College, Xidian University, Xi’an 710071) 《Journal of Electronics(China)》 1998年第1期69-75,共7页
A multisensor distributed extended Kalman filtering algorithm is presented for nonlinear system, in which the dynamic equation of the system and the equations of sensor’s measurements are linearized in the global est... A multisensor distributed extended Kalman filtering algorithm is presented for nonlinear system, in which the dynamic equation of the system and the equations of sensor’s measurements are linearized in the global estimate and global prediction respectively and the suboptimal global estimate based on all available information can be reconstructed from the estimates computed by local sensors based solely on their own local information and transmitted to the data fusion center. An analysis of the properties of the algorithm presented here shows that the global estimate has higher precision than the local one and smaller linearization error than the existing method. Finally, an application of the algorithm to radar/IR tracking of a maneuvering target is illustrated. Simulation results show the effectiveness of the algorithm. 展开更多
关键词 extended KALMAN FILTERING target tracking DISTRIBUTED estimation Data fusion
下载PDF
Waveform Design for Cognitive Radar with Deterministic Extended Targets in the Presence of Clutter
10
作者 Vahid Karimi Reza Mohseni +1 位作者 Yaser Norouzi Mohammad Javad Dehghani 《International Journal of Communications, Network and System Sciences》 2016年第6期250-268,共19页
Adjusting radar transmitted waveform to its environment is one of the most important roles in cognitive radar;having the capability of updating transmitted waveforms in different applications is a key point. It has be... Adjusting radar transmitted waveform to its environment is one of the most important roles in cognitive radar;having the capability of updating transmitted waveforms in different applications is a key point. It has been shown in many studies that if the waveform is designed according to the target and clutter characteristics, the detection performance will improve significantly. The uncertainty of the target radar signatures decreases via maximizing MI and the probability of extended target detection is increases via maximizing SNR. In this paper, a waveform design approach based on maximizing both SNR and MI and with regard to target and clutter shape is presented. The detection performance for proposed waveform is compared with previous proposed waveforms. The present paper compares different scenarios of target and clutter and using the probability of detection as a cost function to investigate the advantages and disadvantages of each waveform in different scenarios which are mainly discussed in this text. The desired waveform for cognitive radar is selected based on simultaneously making compromises between SNR and MI, which plays an important role in cognitive radar systems and based on the assumption addressed in the text, the best waveform transmitted into the environment. 展开更多
关键词 Signal to Noise Ratio Mutual Information Cognitive Radar Deterministic extended target CLUTTER
下载PDF
Differential geometric guidance command with finite time convergence using extended state observer 被引量:5
11
作者 麻毅威 张为华 《Journal of Central South University》 SCIE EI CAS CSCD 2016年第4期859-868,共10页
For improving the performance of differential geometric guidance command(DGGC), a new formation of this guidance law is proposed, which can guarantee the finite time convergence(FTC) of the line of sight(LOS) rate to ... For improving the performance of differential geometric guidance command(DGGC), a new formation of this guidance law is proposed, which can guarantee the finite time convergence(FTC) of the line of sight(LOS) rate to zero or its neighborhood against maneuvering targets in three-dimensional(3D) space. The extended state observer(ESO) is employed to estimate the target acceleration, which makes the new DGGC more applicable to practical interception scenarios. Finally, the effectiveness of this newly proposed guidance command is demonstrated by the numerical simulation results. 展开更多
关键词 missile guidance differential geometric guidance command (DGGC) finite time convergence (FTC) extended state observer (ESO) maneuvering targets
下载PDF
Bayesian target tracking based on particle filter 被引量:10
12
作者 邓小龙 谢剑英 郭为忠 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2005年第3期545-549,共5页
For being able to deal with the nonlinear or non-Gaussian problems, particle filters have been studied by many researchers. Based on particle filter, the extended Kalman filter (EKF) proposal function is applied to ... For being able to deal with the nonlinear or non-Gaussian problems, particle filters have been studied by many researchers. Based on particle filter, the extended Kalman filter (EKF) proposal function is applied to Bayesian target tracking. Markov chain Monte Carlo (MCMC) method, the resampling step, ere novel techniques are also introduced into Bayesian target tracking. And the simulation results confirm the improved particle filter with these techniques outperforms the basic one. 展开更多
关键词 nonlinear/non-Gaussian extended Kalman filter particle filter target tracking proposal function.
下载PDF
Energy-efficient adaptive sensor scheduling for target tracking in wireless sensor networks 被引量:9
13
作者 Wendong XIAO Sen ZHANG +1 位作者 Jianyong LIN Chen Khong THAM 《控制理论与应用(英文版)》 EI 2010年第1期86-92,共7页
Sensor scheduling is essential to collaborative target tracking in wireless sensor networks (WSNs). In the existing works for target tracking in WSNs, such as the information-driven sensor query (IDSQ), the taskin... Sensor scheduling is essential to collaborative target tracking in wireless sensor networks (WSNs). In the existing works for target tracking in WSNs, such as the information-driven sensor query (IDSQ), the tasking sensors are scheduled to maximize the information gain while minimizing the resource cost based on the uniform sampling intervals, ignoring the changing of the target dynamics and the specific desirable tracking goals. This paper proposes a novel energy-efficient adaptive sensor scheduling approach that jointly selects tasking sensors and determines their associated sampling intervals according to the predicted tracking accuracy and tracking energy cost. At each time step, the sensors are scheduled in alternative tracking mode, namely, the fast tracking mode with smallest sampling interval or the tracking maintenance mode with larger sampling interval, according to a specified tracking error threshold. The approach employs an extended Kalman filter (EKF)-based estimation technique to predict the tracking accuracy and adopts an energy consumption model to predict the energy cost. Simulation results demonstrate that, compared to a non-adaptive sensor scheduling approach, the proposed approach can save energy cost significantly without degrading the tracking accuracy. 展开更多
关键词 Wireless sensor network target tracking Sensor scheduling extended Kalman filter Energy efficiency.
下载PDF
Improved Particle Filter for Passive Target Tracking 被引量:3
14
作者 邓小龙 谢剑英 杨煜普 《Journal of Shanghai University(English Edition)》 CAS 2005年第6期534-538,共5页
As a new method for dealing with any nonlinear or non-Ganssian distributions, based on the Monte Carlo methods and Bayesian filtering, particle filters (PF) are favored by researchers and widely applied in many fiel... As a new method for dealing with any nonlinear or non-Ganssian distributions, based on the Monte Carlo methods and Bayesian filtering, particle filters (PF) are favored by researchers and widely applied in many fields. Based on particle filtering, an improved extended Kalman filter (EKF) proposal distribution is presented. Evaluation of the weights is simplified and other improved techniques including the residual resampling step and Markov Chain Monte Carlo method are introduced for target tracking. Performances of the EKF, basic PF and the improved PF are compared in target tracking examples. The simulation results confirm that the improved particle filter outperforms the others. 展开更多
关键词 nonlinear NON-GAUSSIAN particle filter (PF) target tracking extended Kalman filter (EKF).
下载PDF
Cooperative Target Tracking of Multiple Autonomous Surface Vehicles Under Switching Interaction Topologies 被引量:5
15
作者 Lang Ma Yu-Long Wang Qing-Long Han 《IEEE/CAA Journal of Automatica Sinica》 SCIE EI CSCD 2023年第3期673-684,共12页
This paper is concerned with the cooperative target tracking of multiple autonomous surface vehicles(ASVs)under switching interaction topologies.For the target to be tracked,only its position can be measured/received ... This paper is concerned with the cooperative target tracking of multiple autonomous surface vehicles(ASVs)under switching interaction topologies.For the target to be tracked,only its position can be measured/received by some of the ASVs,and its velocity is unavailable to all the ASVs.A distributed extended state observer taking into consideration switching topologies is designed to integrally estimate unknown target dynamics and neighboring ASVs'dynamics.Accordingly,a novel kinematic controller is designed,which takes full advantage of known information and avoids the approximation of some virtual control vectors.Moreover,a disturbance observer is presented to estimate unknown time-varying environmental disturbance.Furthermore,a distributed dynamic controller is designed to regulate the involved ASVs to cooperatively track the target.It enables each ASV to adjust its forces and moments according to the received information from its neighbors.The effectiveness of the derived results is demonstrated through cooperative target tracking performance analysis for a tracking system composed of five interacting ASVs. 展开更多
关键词 Autonomous surface vehicles(ASVs) cooperative target tracking distributed extended state observer switching topologies
下载PDF
Two-step measurement update for extended Kalman filtering
16
作者 ZhangYong'an ZhouDi DuanGuangren 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2005年第1期21-25,共5页
The nonlinear filtering for a class of discrete-time stochastic dynamic systems whose measurement equations contain linear (or universal linearizable) components and nonlinear components which are mutually statistical... The nonlinear filtering for a class of discrete-time stochastic dynamic systems whose measurement equations contain linear (or universal linearizable) components and nonlinear components which are mutually statistical independent is investigated. A two-step measurement update is proposed for the filtering of the systems. The first-step update is a linear (or universal linearization) measurement correction which introduces an intermediate estimate, while the second-step nonlinear linearization update produces the final posterior estimate based on the first-step estimate. Since the first measurement correction is a linear or universal linearization update, it provides an accurate linearization reference point for the second nonlinear measurement update. Two simulation examples show superiority of the new estimation method. 展开更多
关键词 universal linearization extended Kalman filter modified gain extended Kalman filter target tracking.
下载PDF
新生参数未知的ET-GM-PHD编队目标跟踪方法
17
作者 夏沭涛 文云峰 +2 位作者 还迎春 张振杰 李雪腾 《指挥控制与仿真》 2024年第3期109-115,共7页
为了解决多个编队在新生目标参数不明确背景下的目标跟踪问题,提出了一种新生目标相关参数未知的编队目标跟踪算法,它可以有效地提高跟踪性能,从而更好地满足需求。通过采用高斯混合概率假设密度(ET-GM-PHD)滤波框架,对PHD参数进行预测... 为了解决多个编队在新生目标参数不明确背景下的目标跟踪问题,提出了一种新生目标相关参数未知的编队目标跟踪算法,它可以有效地提高跟踪性能,从而更好地满足需求。通过采用高斯混合概率假设密度(ET-GM-PHD)滤波框架,对PHD参数进行预测,进而分析各个高斯分量之间的相关性,从而筛选出最优的量测集合,最终消除大多数的噪声干扰。再利用在编队目标运动区域内量测分布密集的特性,通过目标检测方法找到其位置并跟踪。最后,仿真实验表明,在新生目标相关参数未知的情况下,提出的方法能够快速有效地跟踪编队目标,具有较好的跟踪性能。 展开更多
关键词 编队目标 新生目标相关参数未知 et-GM-PHD滤波 相关性
下载PDF
基于ET-PHD的自适应联合跟踪与分类算法 被引量:2
18
作者 樊鹏飞 李鸿艳 《自动化学报》 EI CSCD 北大核心 2019年第2期349-359,共11页
针对新生目标强度先验未知的扩展目标(Extended target, ET)联合跟踪与分类(Joint tracking and classification,JTC)问题,提出一种基于扩展目标概率假设密度(Extended target-probability hypothesis density, ET-PHD)滤波器的自适应... 针对新生目标强度先验未知的扩展目标(Extended target, ET)联合跟踪与分类(Joint tracking and classification,JTC)问题,提出一种基于扩展目标概率假设密度(Extended target-probability hypothesis density, ET-PHD)滤波器的自适应联合跟踪与分类算法,并给出其高斯混合实现方法.算法利用量测信息生成新生目标强度,在滤波预测阶段对存活目标和新生目标分别按照其类别进行传播,再引入属性量测信息,用位置和属性的联合量测似然函数代替单目标位置似然函数,对预测后所有目标强度进行联合更新,之后按照类别进行高斯项的删减与合并,提取相应类别目标的状态集.仿真结果表明,提出的自适应算法改进了概率假设密度滤波器在扩展目标跟踪中的性能. 展开更多
关键词 扩展目标 联合跟踪与分类 新生目标强度 概率假设密度
下载PDF
基于ET-PHD滤波器和变分贝叶斯近似的扩展目标跟踪算法 被引量:6
19
作者 何祥宇 李静 +1 位作者 杨数强 夏玉杰 《计算机应用》 CSCD 北大核心 2020年第12期3701-3706,共6页
针对未知测量噪声协方差情况下的多扩展目标跟踪问题,利用扩展目标概率假设密度(ET-PHD)滤波器和变分贝叶斯(VB)近似理论,提出了一种标准ET-PHD滤波器的扩展方法及其解析的实现方法。首先,根据标准ETPHD滤波器的目标状态方程和测量方程... 针对未知测量噪声协方差情况下的多扩展目标跟踪问题,利用扩展目标概率假设密度(ET-PHD)滤波器和变分贝叶斯(VB)近似理论,提出了一种标准ET-PHD滤波器的扩展方法及其解析的实现方法。首先,根据标准ETPHD滤波器的目标状态方程和测量方程,定义了目标状态和测量噪声协方差的增广状态变量及二者的联合转移函数;然后,根据标准ET-PHD滤波器,构建了扩展的ET-PHD滤波器的预测和更新公式;最后,在线性高斯假设的条件下,利用高斯和逆伽马(IG)混合分布表示目标的联合后验强度函数,从而给出了扩展ET-PHD滤波器的解析实现。仿真结果表明:所提算法能提供可靠的跟踪结果,可有效地处理未知测量噪声协方差环境中的多扩展目标跟踪问题。 展开更多
关键词 扩展目标跟踪 概率假设密度 随机有限集 变分贝叶斯 噪声协方差
下载PDF
Red/ET重组AHBA生物合成基因簇打靶载体的构建与鉴定 被引量:1
20
作者 王广林 张会图 +1 位作者 张金池 王以光 《安徽师范大学学报(自然科学版)》 CAS 北大核心 2009年第6期569-574,共6页
为确定3-氨基-5-羟基-苯甲酸(AHBA)生物合成基因簇在链霉菌中与次生代谢产物的关系,运用PCR技术,从33株AHBA合酶基因阳性菌株扩增与AHBA生物合成基因簇中编码AHBA合酶(A)、氧化还原酶(O)、磷酸化酶(P)基因,获得24株AOP基因阳性菌株.根... 为确定3-氨基-5-羟基-苯甲酸(AHBA)生物合成基因簇在链霉菌中与次生代谢产物的关系,运用PCR技术,从33株AHBA合酶基因阳性菌株扩增与AHBA生物合成基因簇中编码AHBA合酶(A)、氧化还原酶(O)、磷酸化酶(P)基因,获得24株AOP基因阳性菌株.根据靶基因A基因下游和P基因上游同源序列设计50 bp引物,中间插入卡那霉素抗性基因的DNA片段,进行PCR,获得外源DNA片段.经过电转化,将外源DNA片段和pKC1139-AOP重组质粒共转入含重组酶质粒大肠杆菌HS996/pSC101-BAD-gba-(Tet).在Red重组酶的作用下,外源DNA片段与重组质粒pKC1139-AOP上的AHBA基因簇的同源区域重组,构建了AHBA基因簇打靶载体.研究显示了Red/ET重组工作效率高、操作简单、精确的优点,可大大缩短构建打靶载体的时间. 展开更多
关键词 Red/et重组 AHBA基因簇 打靶载体 链霉菌
下载PDF
上一页 1 2 35 下一页 到第
使用帮助 返回顶部