Objective:To establish extensively drug-resistant Pseudomonas aeruginosa(XDR-PA)infection-induced pneumonia model in rats.Methods:Twenty-four male SD rats were randomly divided into blank group,low bacterial group,med...Objective:To establish extensively drug-resistant Pseudomonas aeruginosa(XDR-PA)infection-induced pneumonia model in rats.Methods:Twenty-four male SD rats were randomly divided into blank group,low bacterial group,medium bacterial group,and high bacterial group.The low,medium and high bacterial groups were given intratracheal instillation of 0.1 mL of bacterial suspension(bacterial concentration in turn is 7.5×10^(9),3×10^(10),6×10^(10)CFU/mL),while the blank group were given the same volume of sterile normal saline.After modeling,the general conditions of rats in each group were observed,including mental state,hair,respiration,activity,eating,weight,and the survival curve was drawn.The pathological characteristics of lung tissue and the infiltration of inflammatory cells were observed.Pathogenic identification of each group was carried out by bacterial culture of lung tissue homogenate.Results:The general state of the blank group was normal,and the rats in other groups showed signs of mental depression,bristling,shortness of breath,even oral and nasal bleeding,decreased food intake and activity,and significant weight loss,and different degrees of death within 48 hours,the difference was statistically significant(P<0.05).Pathological results showed that the alveolar structure of rats in the blank group was complete,and the alveolar space was clear without exudation.The lung tissue of the low and medium bacterial groups showed obvious inflammatory cell infiltration,alveolar structure destruction,alveolar septum thickening,interstitial edema,but the pathological damage of the medium group was more severe,with a mortality rate of up to 50%,and the mortality rate of the low bacterial group was 17%.In the high bacterial group,red blood cells,inflammatory cells and a large amount of fibrin-like exudation can be seen in the alveolar space,which has the pathological characteristics of acute respiratory failure,and the mortality rate is as high as 67%.The results of bacterial culture of lung tissue homogenate showed that the blank group had no bacterial colonies,while PA colony growth can be seen in low,medium and high bacterial groups.Conclusion:9 Intratracheal instillation of low bacterial count(0.1 mL of 7.5×10^(9) CFU/mL)XDR-PA bacterial suspension can successfully construct a rat pneumonia model of XDR-PA infection.展开更多
目的:探究ADOPT问题解决模式护理对大面积烧伤患者伤残接受度、创面愈合及美观满意度的影响。方法:前瞻性选择2020年1月-2022年12月笔者医院收治的患者460例大面积烧伤患者为研究对象,将2020年1月-2021年3月收治的患者纳入对照组,2021年...目的:探究ADOPT问题解决模式护理对大面积烧伤患者伤残接受度、创面愈合及美观满意度的影响。方法:前瞻性选择2020年1月-2022年12月笔者医院收治的患者460例大面积烧伤患者为研究对象,将2020年1月-2021年3月收治的患者纳入对照组,2021年4月-2022年12月收治的纳入观察组,对照组采用大面积烧伤后常规护理,观察组采用ADOPT问题解决模式护理,持续护理3个月。分别于干预前和干预3个月后,采用伤残接受度量表(Acceptance of disability scale,AODS)评估患者伤残接受度,采用视觉模拟评分(Visual analogue scale,VAS)评估患者创面疼痛程度,采用简明烧伤健康量表(Burn specific health scale,BSHS-A)评估患者生活质量;干预4周后,计算创面愈合率;干预3个月后,采用(Vancouver scar scale,VSS)评估患者创面愈合后瘢痕增生情况,采用美观满意度评分表评估患者美观满意度;记录创面愈合时间。结果:干预后,观察组患者AODS评分、创面覆盖率、美观满意度、BSHS-A评分均明显高于对照组,创面愈合时间明显短于对照组,VAS评分和VSS评分明显低于对照组(P<0.05)。结论:ADOPT问题解决模式能够提高大面积烧伤患者伤残接受度和美观满意度,有利于创面愈合,患者生活质量也得以显著提高。展开更多
基金Science and Technology Projects in Key Fields of Traditional Chinese Medicine in Tianjin(No.2021010)Discipline Development Fund of First Teaching Hospital of Tianjin University of Traditional Chinese Medicine(No.XKJJ201734)。
文摘Objective:To establish extensively drug-resistant Pseudomonas aeruginosa(XDR-PA)infection-induced pneumonia model in rats.Methods:Twenty-four male SD rats were randomly divided into blank group,low bacterial group,medium bacterial group,and high bacterial group.The low,medium and high bacterial groups were given intratracheal instillation of 0.1 mL of bacterial suspension(bacterial concentration in turn is 7.5×10^(9),3×10^(10),6×10^(10)CFU/mL),while the blank group were given the same volume of sterile normal saline.After modeling,the general conditions of rats in each group were observed,including mental state,hair,respiration,activity,eating,weight,and the survival curve was drawn.The pathological characteristics of lung tissue and the infiltration of inflammatory cells were observed.Pathogenic identification of each group was carried out by bacterial culture of lung tissue homogenate.Results:The general state of the blank group was normal,and the rats in other groups showed signs of mental depression,bristling,shortness of breath,even oral and nasal bleeding,decreased food intake and activity,and significant weight loss,and different degrees of death within 48 hours,the difference was statistically significant(P<0.05).Pathological results showed that the alveolar structure of rats in the blank group was complete,and the alveolar space was clear without exudation.The lung tissue of the low and medium bacterial groups showed obvious inflammatory cell infiltration,alveolar structure destruction,alveolar septum thickening,interstitial edema,but the pathological damage of the medium group was more severe,with a mortality rate of up to 50%,and the mortality rate of the low bacterial group was 17%.In the high bacterial group,red blood cells,inflammatory cells and a large amount of fibrin-like exudation can be seen in the alveolar space,which has the pathological characteristics of acute respiratory failure,and the mortality rate is as high as 67%.The results of bacterial culture of lung tissue homogenate showed that the blank group had no bacterial colonies,while PA colony growth can be seen in low,medium and high bacterial groups.Conclusion:9 Intratracheal instillation of low bacterial count(0.1 mL of 7.5×10^(9) CFU/mL)XDR-PA bacterial suspension can successfully construct a rat pneumonia model of XDR-PA infection.
文摘目的:探究ADOPT问题解决模式护理对大面积烧伤患者伤残接受度、创面愈合及美观满意度的影响。方法:前瞻性选择2020年1月-2022年12月笔者医院收治的患者460例大面积烧伤患者为研究对象,将2020年1月-2021年3月收治的患者纳入对照组,2021年4月-2022年12月收治的纳入观察组,对照组采用大面积烧伤后常规护理,观察组采用ADOPT问题解决模式护理,持续护理3个月。分别于干预前和干预3个月后,采用伤残接受度量表(Acceptance of disability scale,AODS)评估患者伤残接受度,采用视觉模拟评分(Visual analogue scale,VAS)评估患者创面疼痛程度,采用简明烧伤健康量表(Burn specific health scale,BSHS-A)评估患者生活质量;干预4周后,计算创面愈合率;干预3个月后,采用(Vancouver scar scale,VSS)评估患者创面愈合后瘢痕增生情况,采用美观满意度评分表评估患者美观满意度;记录创面愈合时间。结果:干预后,观察组患者AODS评分、创面覆盖率、美观满意度、BSHS-A评分均明显高于对照组,创面愈合时间明显短于对照组,VAS评分和VSS评分明显低于对照组(P<0.05)。结论:ADOPT问题解决模式能够提高大面积烧伤患者伤残接受度和美观满意度,有利于创面愈合,患者生活质量也得以显著提高。
