目的:观察参芪泄浊饮对慢性肾衰大鼠肾组织胶原蛋白Ⅲ(ColⅢ)、胶原蛋白Ⅳ(ColⅣ)表达的影响。方法:采用Yokozawa法复制慢性肾衰大鼠模型,随机分为参芪泄浊饮高剂量组、常规剂量组、氯沙坦组、造模组及正常组,分别予参芪泄浊饮水煎液20...目的:观察参芪泄浊饮对慢性肾衰大鼠肾组织胶原蛋白Ⅲ(ColⅢ)、胶原蛋白Ⅳ(ColⅣ)表达的影响。方法:采用Yokozawa法复制慢性肾衰大鼠模型,随机分为参芪泄浊饮高剂量组、常规剂量组、氯沙坦组、造模组及正常组,分别予参芪泄浊饮水煎液20、10 m L·kg-1·d-1、氯沙坦9 mg·kg-1·d-1,造模组与正常组均予纯净水10 m L·kg-1·d-1,给药8周,酶法测量血肌酐(Scr)、尿素氮(BUN),并采用免疫组化检测肾脏ColⅢ、Ⅳ的表达。结果:1高剂量组Scr水平明显低于常规剂量组、氯沙坦组及造模组;常规剂量组与氯沙坦组无明显差别,均低于造模组;2ColⅢ表达水平:高剂量组<常规剂量组<氯沙坦组<造模组,相邻组间均存在显著统计差异(P<0.01);3ColⅣ表达水平除高剂量组与常规剂量组间不存在明显差异,其余变化趋势同ColⅢ。结论:参芪泄浊饮可通过减少细胞外基质ColⅢ、Ⅳ在肾组织过度沉积来缓解氮质血症,保护肾功能。展开更多
Over last 20 years, extracellular matrices have been shown to be useful in promoting tissue regeneration. Recently, they have been used and have had success in achieving neurogenesis. Recent developments in extracellu...Over last 20 years, extracellular matrices have been shown to be useful in promoting tissue regeneration. Recently, they have been used and have had success in achieving neurogenesis. Recent developments in extracellular matrix design have allowed their successful in vivo incorporation to engender an environment favorable for neural regeneration in animal models. Promising treatments under investigation include manipulation of the intrinsic extracellular matrix and incorporation of engineered naometer-sized scaffolds through which inhibition of molecules serving as barriers to neuroregeneration and delivery of neurotrophic factors and/or cells for successful tissue regeneration can be achieved. Further understanding of the changes incurred within the extracellular matrix following central nervous system injury will undoubtedly help design a clinically efficacious extracellular matrix scaffold that can mitigate or reverse neural degeneration in the clinical setting.展开更多
Objective To optimize the therapeutic dosage of tetrandrine （Tet） in rat hepatic fibrosis roodel. Methods 50 Wistar rats were divided into 5 groups at random including normal control, model control, Tettreated model...Objective To optimize the therapeutic dosage of tetrandrine （Tet） in rat hepatic fibrosis roodel. Methods 50 Wistar rats were divided into 5 groups at random including normal control, model control, Tettreated model groups of 10mg· kg^ - 1· d^ - 1, 5mg· kg^ - 1· d^ - 1 and 2.5mg· kg^ - 1· d^ - 1（ n = 10 in each group ）. All rats, except for the normal controls, were injected with axenic porcine serum （0. 5ml each time, twice a week） intraperitoneally for 8 weeks to establish hepatic fibrosis. After the 8th week, rats of Tet-treated model groups were given by gavage once a day with different doses of Tet for another 8 weeks. Then the liver function, serum levels of hyaluronic acid （ HA ）, laminin （ LM）, and procollagen type Ⅲ （PCⅢ） were tested. Collagen type 1 and Ⅲ, pathological changes in liver tissue were also assessed. Results Most indices of liver function including alanine minotransferase （ALT）, aspartate aminotransferase （AST）, albumin （ ALB）, albumin/globulin ratio （ A/G） and alkaline phosphatase （ALP） improved significantly in Tet-treated groups with the exception of γ-glutamyl transpeptidase （γ- GT） and total bilirubin （TBIL）. Secondly, markedly lowered levels of HA, LM and collagen type I, III were also detected by radioimmunology and immunohistochemistry in the 5 mg· kg^ - 1· d^ - 1 Tet-treated model group. Moreover, pathologi- cal findings confirmed the statistically significant improvement in hepatofibrotic degree resulted from the treatment of 5mg· kg^ - 1· d^ - 1 rather than other doses of Tet. Conclusion For experimental Wistar rats, Tet exhibited an anti-hepatofibrotic action in doses within the range of 2.5mg· kg^ - 1· d^ - 1 to 10mg· kg^ - 1· d^ - 1 and 5mg· kg^ - 1· d^ - 1 may be the optimum one among all doses.展开更多
文摘目的:观察参芪泄浊饮对慢性肾衰大鼠肾组织胶原蛋白Ⅲ(ColⅢ)、胶原蛋白Ⅳ(ColⅣ)表达的影响。方法:采用Yokozawa法复制慢性肾衰大鼠模型,随机分为参芪泄浊饮高剂量组、常规剂量组、氯沙坦组、造模组及正常组,分别予参芪泄浊饮水煎液20、10 m L·kg-1·d-1、氯沙坦9 mg·kg-1·d-1,造模组与正常组均予纯净水10 m L·kg-1·d-1,给药8周,酶法测量血肌酐(Scr)、尿素氮(BUN),并采用免疫组化检测肾脏ColⅢ、Ⅳ的表达。结果:1高剂量组Scr水平明显低于常规剂量组、氯沙坦组及造模组;常规剂量组与氯沙坦组无明显差别,均低于造模组;2ColⅢ表达水平:高剂量组<常规剂量组<氯沙坦组<造模组,相邻组间均存在显著统计差异(P<0.01);3ColⅣ表达水平除高剂量组与常规剂量组间不存在明显差异,其余变化趋势同ColⅢ。结论:参芪泄浊饮可通过减少细胞外基质ColⅢ、Ⅳ在肾组织过度沉积来缓解氮质血症,保护肾功能。
文摘Over last 20 years, extracellular matrices have been shown to be useful in promoting tissue regeneration. Recently, they have been used and have had success in achieving neurogenesis. Recent developments in extracellular matrix design have allowed their successful in vivo incorporation to engender an environment favorable for neural regeneration in animal models. Promising treatments under investigation include manipulation of the intrinsic extracellular matrix and incorporation of engineered naometer-sized scaffolds through which inhibition of molecules serving as barriers to neuroregeneration and delivery of neurotrophic factors and/or cells for successful tissue regeneration can be achieved. Further understanding of the changes incurred within the extracellular matrix following central nervous system injury will undoubtedly help design a clinically efficacious extracellular matrix scaffold that can mitigate or reverse neural degeneration in the clinical setting.
文摘Objective To optimize the therapeutic dosage of tetrandrine （Tet） in rat hepatic fibrosis roodel. Methods 50 Wistar rats were divided into 5 groups at random including normal control, model control, Tettreated model groups of 10mg· kg^ - 1· d^ - 1, 5mg· kg^ - 1· d^ - 1 and 2.5mg· kg^ - 1· d^ - 1（ n = 10 in each group ）. All rats, except for the normal controls, were injected with axenic porcine serum （0. 5ml each time, twice a week） intraperitoneally for 8 weeks to establish hepatic fibrosis. After the 8th week, rats of Tet-treated model groups were given by gavage once a day with different doses of Tet for another 8 weeks. Then the liver function, serum levels of hyaluronic acid （ HA ）, laminin （ LM）, and procollagen type Ⅲ （PCⅢ） were tested. Collagen type 1 and Ⅲ, pathological changes in liver tissue were also assessed. Results Most indices of liver function including alanine minotransferase （ALT）, aspartate aminotransferase （AST）, albumin （ ALB）, albumin/globulin ratio （ A/G） and alkaline phosphatase （ALP） improved significantly in Tet-treated groups with the exception of γ-glutamyl transpeptidase （γ- GT） and total bilirubin （TBIL）. Secondly, markedly lowered levels of HA, LM and collagen type I, III were also detected by radioimmunology and immunohistochemistry in the 5 mg· kg^ - 1· d^ - 1 Tet-treated model group. Moreover, pathologi- cal findings confirmed the statistically significant improvement in hepatofibrotic degree resulted from the treatment of 5mg· kg^ - 1· d^ - 1 rather than other doses of Tet. Conclusion For experimental Wistar rats, Tet exhibited an anti-hepatofibrotic action in doses within the range of 2.5mg· kg^ - 1· d^ - 1 to 10mg· kg^ - 1· d^ - 1 and 5mg· kg^ - 1· d^ - 1 may be the optimum one among all doses.
