Extracellular polymeric substances (EPS) are the predominant constituents of activated sludge and represent up to 80% of the mass of activated sludge. They play a crucial role in the flocculation, settling and dewat...Extracellular polymeric substances (EPS) are the predominant constituents of activated sludge and represent up to 80% of the mass of activated sludge. They play a crucial role in the flocculation, settling and dewatering of activated sludge. Furthermore, EPS also show great efficiency in binding heavy metals. So EPS are key factors influencing reduction in sludge volume and mass, as well as activity and utilization of sludge. EPS are of considerable environmental interest and hundreds of articles on EPS have been published abroad, while information on EPS in China is limited. In this paper, results of over 60 publications related to constituents and characteristics of EPS and their influences on flocculation, settling and dewatering of sludge are compiled and analyzed. Metal-binding ability of EPS is also discussed, together with a brief consideration of possible research interests in the future.展开更多
Although many studies have found that cadmium(Cd)can be toxic to microalgae,only a few reports focused on the role of extracellular polymeric substances(EPS)in Cd(Ⅱ)detoxification.The biochemical and physiological en...Although many studies have found that cadmium(Cd)can be toxic to microalgae,only a few reports focused on the role of extracellular polymeric substances(EPS)in Cd(Ⅱ)detoxification.The biochemical and physiological endpoints of Microcystis aeruginosa,including the composition and functional groups of soluble EPS(SL-EPS),loosely bound EPS(LB-EPS),and tightly bound EPS(TB-EPS),were detected to elucidate the toxicity and detoxification mechanisms of Cd(Ⅱ)for cyanobacteria.Toxicological and physiological assays on M.aeruginosa showed that the 0.25-mg/L Cd(Ⅱ)resulted in a larger inhibition on growth and F_(v)/F_(m).Nevertheless,Cd(Ⅱ)significantly induced much higher contents of superoxide dismutase(SOD),intracellular microcystin LR(MC-LR),extracellular MC-LR,and EPS.Scanning electron microscopy with energy dispersive X-ray spectroscopy confirmed that Cd(Ⅱ)was absorbed into the EPS layer.Fourier transform infrared spectrum analysis revealed that the functional groups bound with Cd(Ⅱ)of algae biomass,SL-EPS,LB-EPS,and TB-EPS were somewhat different.The C=O/C=N groups ofδ-lactam or protein were their prominent functional groups,suggesting that amide or proteins in the EPS played a key role in the adsorption in Cd(Ⅱ).The concentration of 0.25 mg/L of Cd(Ⅱ)may change the chemical structure of EPS by altering the production of protein-like substances containing tryptophan.This study indicated that M.aeruginosa could detoxify Cd(Ⅱ)stress via induction of antioxidant capacity(higher SOD activity and MC synthesis),EPS production,and modification in chemical structure of EPS.展开更多
This paper describes the investigation of the secretion of extracellular polymeric substances(EPS)by an extremely thermoacidophilic archaea,Metallosphaera sedula(M.sedula),during the bioleaching of pyrite under differ...This paper describes the investigation of the secretion of extracellular polymeric substances(EPS)by an extremely thermoacidophilic archaea,Metallosphaera sedula(M.sedula),during the bioleaching of pyrite under different temperatures and discusses the relationship among the EPS secretion,its heat resistance,and its ability to bioleach pyrite.The investigation results indicate that the amount of extracellular proteins is significantly higher than the amount of extracellular polysaccharides in the extracted EPS whether free cells or attached cells;these results are quite different from the behavior of mesophilic Acidithiobacillus ferrooxidans.Although the growth of M.sedula is inhibited at 80℃,the bioleaching ability of M.sedula is only slightly lower than that at the optimum growth temperature of 72℃ because of the heat resistance mechanism based on EPS secretion.The secretion of more extracellular proteins is an important heat resistance mechanism of M.sedula.展开更多
Colony formation of cyanobacteria is crucial for the formation of surface blooms in lakes.However,the underlying mechanisms of colony formation involving in physiological and cell surface characteristics remain to not...Colony formation of cyanobacteria is crucial for the formation of surface blooms in lakes.However,the underlying mechanisms of colony formation involving in physiological and cell surface characteristics remain to not well be established.Six cyanobacterial Microcystis strains(including both unicellular and colonial ones)were employed to estimate the influences of their physiological traits and the composition of extracellular polymeric substances(EPS)on colony or aggregate formation.Results show that raising the number of the photosynthetic reaction center and light-harvesting antenna in the PSII and reducing the growth rate were the major physiological strategies of Microcystis to produce excess EPS enhancing colony formation.Tightly bound EPS(T-EPS)was responsible for colony formation,which approximately accounted for 50%of the total amount of EPS.Five fluorescent components(protein-,tryptophan-,and tyrosine-like components and two humic-like components)were found in the T-EPS,although the amounts of these components varied with strains.Importantly,colonial strains contained much higher tyrosine-like substances than unicellular ones.We suggest that tyrosine-like substances might serve as a crosslinking agent to connect other polymers in EPS(e.g.,proteins or polysaccharides)for colony formation.Our findings identified key physiological traits and chemical components of EPS for colony formation in Microcystis,which can contribute to a better understanding on the formation of Microcystis blooms.展开更多
研究了序批式反应器(SBR)内好氧颗粒污泥在不同有机负荷(1.6 kg COD/(m3.d)和8 kg COD/(m3.d))和不同含盐(2.5%和5.0%)条件下胞外聚合物(EPS)的变化情况,并对EPS、比耗氧速率(SOUR)和孔隙率之间的关系作了进一步探讨。结果表明:在SBR反...研究了序批式反应器(SBR)内好氧颗粒污泥在不同有机负荷(1.6 kg COD/(m3.d)和8 kg COD/(m3.d))和不同含盐(2.5%和5.0%)条件下胞外聚合物(EPS)的变化情况,并对EPS、比耗氧速率(SOUR)和孔隙率之间的关系作了进一步探讨。结果表明:在SBR反应器中,负荷较含盐量对好氧颗粒污泥EPS变化的影响要显著,且EPS糖成分和SOUR之间、EPS蛋白成分和孔隙率之间均呈负相关。另外,高负荷条件下的系统稳定性要优于低负荷。展开更多
Extracellular polymeric substances(EPS)are an importantmedium for communication and material exchange between iron-oxidizing bacteria and the external environment and could induce the iron(oxyhydr)oxides production wh...Extracellular polymeric substances(EPS)are an importantmedium for communication and material exchange between iron-oxidizing bacteria and the external environment and could induce the iron(oxyhydr)oxides production which reduced arsenic(As)availability.The main component of EPS secreted by iron-oxidizing bacteria(Ochrobactrum EEELCW01)was composed of polysaccharides(150.76-165.33 mg/g DW)followed by considerably smaller amounts of proteins(12.98–16.12 mg/g DW).Low concentrations of As(100 or 500μmol/L)promoted the amount of EPS secretion.FTIR results showed that EPS was composed of polysaccharides,proteins,and a miniscule amount of nucleic acids.The functional groups including-COOH,-OH,-NH,-C=O,and-C-O played an important role in the adsorption of As.XPS results showed that As was bound to EPS in the form of As3+.With increasing As concentration,the proportion of As3+adsorbed on EPS increased.Ferrihydrite with a weak crystalline state was only produced in the system at 6 hr during the mineralization process of Ochrobactrum sp.At day 8,the minerals were composed of goethite,galena,and siderite.With the increasing mineralization time,the main mineral phases were transformed from weakly crystalline hydrous iron ore into higher crystallinity siderite(FeCO_(3))or goethite(α-FeOOH),and the specific surface area and active sites of minerals were reduced.It can be seen from the distribution of As elements that As is preferentially adsorbed on the edges of iron minerals.This study is potential to understand the biomineralizationmechanism of iron-oxidizing bacteria and As remediation in the environment.展开更多
基金The National Natural Science Foundation of China (No. 50578053) and the Harbin Young Scientist Fund (No. 2003AFXXJ025)
文摘Extracellular polymeric substances (EPS) are the predominant constituents of activated sludge and represent up to 80% of the mass of activated sludge. They play a crucial role in the flocculation, settling and dewatering of activated sludge. Furthermore, EPS also show great efficiency in binding heavy metals. So EPS are key factors influencing reduction in sludge volume and mass, as well as activity and utilization of sludge. EPS are of considerable environmental interest and hundreds of articles on EPS have been published abroad, while information on EPS in China is limited. In this paper, results of over 60 publications related to constituents and characteristics of EPS and their influences on flocculation, settling and dewatering of sludge are compiled and analyzed. Metal-binding ability of EPS is also discussed, together with a brief consideration of possible research interests in the future.
基金Supported by the National Natural Science Foundation of China(Nos.31800457,32170383)。
文摘Although many studies have found that cadmium(Cd)can be toxic to microalgae,only a few reports focused on the role of extracellular polymeric substances(EPS)in Cd(Ⅱ)detoxification.The biochemical and physiological endpoints of Microcystis aeruginosa,including the composition and functional groups of soluble EPS(SL-EPS),loosely bound EPS(LB-EPS),and tightly bound EPS(TB-EPS),were detected to elucidate the toxicity and detoxification mechanisms of Cd(Ⅱ)for cyanobacteria.Toxicological and physiological assays on M.aeruginosa showed that the 0.25-mg/L Cd(Ⅱ)resulted in a larger inhibition on growth and F_(v)/F_(m).Nevertheless,Cd(Ⅱ)significantly induced much higher contents of superoxide dismutase(SOD),intracellular microcystin LR(MC-LR),extracellular MC-LR,and EPS.Scanning electron microscopy with energy dispersive X-ray spectroscopy confirmed that Cd(Ⅱ)was absorbed into the EPS layer.Fourier transform infrared spectrum analysis revealed that the functional groups bound with Cd(Ⅱ)of algae biomass,SL-EPS,LB-EPS,and TB-EPS were somewhat different.The C=O/C=N groups ofδ-lactam or protein were their prominent functional groups,suggesting that amide or proteins in the EPS played a key role in the adsorption in Cd(Ⅱ).The concentration of 0.25 mg/L of Cd(Ⅱ)may change the chemical structure of EPS by altering the production of protein-like substances containing tryptophan.This study indicated that M.aeruginosa could detoxify Cd(Ⅱ)stress via induction of antioxidant capacity(higher SOD activity and MC synthesis),EPS production,and modification in chemical structure of EPS.
基金financially supported by the National Natural Science Foundation of China (Nos. 31470230, 51320105006, 51604308, and 31100173)the Youth Talent Foundation of Hunan Province of China (No. 2017RS3003)the National Science Foundation of Hunan Province of China (No. 2018JJ2486)
文摘This paper describes the investigation of the secretion of extracellular polymeric substances(EPS)by an extremely thermoacidophilic archaea,Metallosphaera sedula(M.sedula),during the bioleaching of pyrite under different temperatures and discusses the relationship among the EPS secretion,its heat resistance,and its ability to bioleach pyrite.The investigation results indicate that the amount of extracellular proteins is significantly higher than the amount of extracellular polysaccharides in the extracted EPS whether free cells or attached cells;these results are quite different from the behavior of mesophilic Acidithiobacillus ferrooxidans.Although the growth of M.sedula is inhibited at 80℃,the bioleaching ability of M.sedula is only slightly lower than that at the optimum growth temperature of 72℃ because of the heat resistance mechanism based on EPS secretion.The secretion of more extracellular proteins is an important heat resistance mechanism of M.sedula.
基金Supported by the National Natural Science Foundation of China(No.32071569)the Scientific Instruments and Equipment Development Project+2 种基金Chinese Academy of Sciences,2020(No.YJKYYQ20200048)the Fundamental Research Funds for the Central Universities(No.B210202010)the China Postdoctoral Foundation(No.2020M681472)。
文摘Colony formation of cyanobacteria is crucial for the formation of surface blooms in lakes.However,the underlying mechanisms of colony formation involving in physiological and cell surface characteristics remain to not well be established.Six cyanobacterial Microcystis strains(including both unicellular and colonial ones)were employed to estimate the influences of their physiological traits and the composition of extracellular polymeric substances(EPS)on colony or aggregate formation.Results show that raising the number of the photosynthetic reaction center and light-harvesting antenna in the PSII and reducing the growth rate were the major physiological strategies of Microcystis to produce excess EPS enhancing colony formation.Tightly bound EPS(T-EPS)was responsible for colony formation,which approximately accounted for 50%of the total amount of EPS.Five fluorescent components(protein-,tryptophan-,and tyrosine-like components and two humic-like components)were found in the T-EPS,although the amounts of these components varied with strains.Importantly,colonial strains contained much higher tyrosine-like substances than unicellular ones.We suggest that tyrosine-like substances might serve as a crosslinking agent to connect other polymers in EPS(e.g.,proteins or polysaccharides)for colony formation.Our findings identified key physiological traits and chemical components of EPS for colony formation in Microcystis,which can contribute to a better understanding on the formation of Microcystis blooms.
文摘研究了序批式反应器(SBR)内好氧颗粒污泥在不同有机负荷(1.6 kg COD/(m3.d)和8 kg COD/(m3.d))和不同含盐(2.5%和5.0%)条件下胞外聚合物(EPS)的变化情况,并对EPS、比耗氧速率(SOUR)和孔隙率之间的关系作了进一步探讨。结果表明:在SBR反应器中,负荷较含盐量对好氧颗粒污泥EPS变化的影响要显著,且EPS糖成分和SOUR之间、EPS蛋白成分和孔隙率之间均呈负相关。另外,高负荷条件下的系统稳定性要优于低负荷。
基金supported by the National Natural Science Foundation of China(No.42177392)the Dean’s Research Fund 2020/21(No.04626)of the Education University of Hong Kong.
文摘Extracellular polymeric substances(EPS)are an importantmedium for communication and material exchange between iron-oxidizing bacteria and the external environment and could induce the iron(oxyhydr)oxides production which reduced arsenic(As)availability.The main component of EPS secreted by iron-oxidizing bacteria(Ochrobactrum EEELCW01)was composed of polysaccharides(150.76-165.33 mg/g DW)followed by considerably smaller amounts of proteins(12.98–16.12 mg/g DW).Low concentrations of As(100 or 500μmol/L)promoted the amount of EPS secretion.FTIR results showed that EPS was composed of polysaccharides,proteins,and a miniscule amount of nucleic acids.The functional groups including-COOH,-OH,-NH,-C=O,and-C-O played an important role in the adsorption of As.XPS results showed that As was bound to EPS in the form of As3+.With increasing As concentration,the proportion of As3+adsorbed on EPS increased.Ferrihydrite with a weak crystalline state was only produced in the system at 6 hr during the mineralization process of Ochrobactrum sp.At day 8,the minerals were composed of goethite,galena,and siderite.With the increasing mineralization time,the main mineral phases were transformed from weakly crystalline hydrous iron ore into higher crystallinity siderite(FeCO_(3))or goethite(α-FeOOH),and the specific surface area and active sites of minerals were reduced.It can be seen from the distribution of As elements that As is preferentially adsorbed on the edges of iron minerals.This study is potential to understand the biomineralizationmechanism of iron-oxidizing bacteria and As remediation in the environment.