USING a magnetic method which was designed for identification of CaMBPs according to theprinciples of affinity chromatography, we were able to isolate 3 CaMBPs with calmodulin(CaM) binding activity from the cell wall ...USING a magnetic method which was designed for identification of CaMBPs according to theprinciples of affinity chromatography, we were able to isolate 3 CaMBPs with calmodulin(CaM) binding activity from the cell wall fractions of transgenic tobacco. After SDS-PAGEand CaM gel overlay analysis, we found that CaMBPs were retained and the isolation is effi-展开更多
As a possible peptide primary messenger, extracellular calmodulin (CaM) may regulate processes such as cell proliferation, pollen germination and expression of some genes. Stomata open or close quickly in response to ...As a possible peptide primary messenger, extracellular calmodulin (CaM) may regulate processes such as cell proliferation, pollen germination and expression of some genes. Stomata open or close quickly in response to environmental stimuli. CaM was found to be extracellular both in guard cells of broad bean leaves and in epidermal cells by immuno-electron microscopy and immuno-fluorescence microscopy techniques. Exogenous purified CaM enhanced stomatal closure and inhibited stomatal opening with an optimal concentration of 10(-8) mol/L; CaM antagonist W7-agarose and anti-CaM serum, which were membrane-impermeable macromolecules, inhibited stomatal closure and promoted stomatal opening. All these data showed that endogenous extracellular CaM. of guard cells did promote stomatal closure and inhibit stomatal opening, and could he active only outside the cells. Therefore under natural conditions, the endogenous extracellular CaM of guard cells might regulate stomatal movements as a primary messenger together with other signal molecules, and might be an important linkage between environmental stimuli and cell responses.展开更多
文摘USING a magnetic method which was designed for identification of CaMBPs according to theprinciples of affinity chromatography, we were able to isolate 3 CaMBPs with calmodulin(CaM) binding activity from the cell wall fractions of transgenic tobacco. After SDS-PAGEand CaM gel overlay analysis, we found that CaMBPs were retained and the isolation is effi-
文摘As a possible peptide primary messenger, extracellular calmodulin (CaM) may regulate processes such as cell proliferation, pollen germination and expression of some genes. Stomata open or close quickly in response to environmental stimuli. CaM was found to be extracellular both in guard cells of broad bean leaves and in epidermal cells by immuno-electron microscopy and immuno-fluorescence microscopy techniques. Exogenous purified CaM enhanced stomatal closure and inhibited stomatal opening with an optimal concentration of 10(-8) mol/L; CaM antagonist W7-agarose and anti-CaM serum, which were membrane-impermeable macromolecules, inhibited stomatal closure and promoted stomatal opening. All these data showed that endogenous extracellular CaM. of guard cells did promote stomatal closure and inhibit stomatal opening, and could he active only outside the cells. Therefore under natural conditions, the endogenous extracellular CaM of guard cells might regulate stomatal movements as a primary messenger together with other signal molecules, and might be an important linkage between environmental stimuli and cell responses.
