Duchesnea indica (Andr.) Focke has been traditionally used to treat cancer in Asian countries for centuries. In the present study, transplanted U14 cervical cancer mouse model was used to evaluate the antitumor and im...Duchesnea indica (Andr.) Focke has been traditionally used to treat cancer in Asian countries for centuries. In the present study, transplanted U14 cervical cancer mouse model was used to evaluate the antitumor and immunomodulatory activity of Duchesnea phenolic fraction (DPF). ELISA and RIA assay were employed to measured the serum concentration of Th1/Th2 cytokines (IL-2, IL-4, IFN-γ and TNF-α). Administration with 0.25 g/kg, 0.5 g/kg and 1 g/kg DPF significantly reduced the tumor weight by 34.37%, 43.89% and 56.28%, respectively, as compared to the tumor control group. Furthermore, the serum level of IL-2, IFN-γ and TNF-α increased and IL-4 level decreased in a dose-dependent manner during DPF treatment, indicating that the antitumor activity of DPF may be associated with the decrease of TNF-α level and restoration of the balance of Th1/Th2 cell responses. These data suggested that DPF, a mixture of plant polyphenols, had potent anticancer activity which was in part accomplished by its immunomodulatory ability.展开更多
【目的】研究Cd污染对蛇泡草生理生化特性指标的影响,为重金属对中草药的毒害作用研究提供参考。【方法】采用盆栽模拟试验,待蛇泡草幼苗待生长稳定后,以50 m L/d的量浇灌不同浓度(11、22、45、90和134 mg/kg)的Cd2+溶液,记录蛇泡草5、3...【目的】研究Cd污染对蛇泡草生理生化特性指标的影响,为重金属对中草药的毒害作用研究提供参考。【方法】采用盆栽模拟试验,待蛇泡草幼苗待生长稳定后,以50 m L/d的量浇灌不同浓度(11、22、45、90和134 mg/kg)的Cd2+溶液,记录蛇泡草5、30和45 d的生长情况,同时测定其的可溶性总糖含量、总黄酮含量、及抗氧化酶活性。【结果】随Cd溶液浓度的增加,蛇泡草体内Cd含量不断增大,同时蛇泡草叶片、茎部和根部的可溶性总糖、总黄酮、及抗氧化酶活性均呈现出不断降低的趋势。【结论】Cd较易在蛇泡草体内积累,并会破坏植物的新成代谢和正常生长,导致各项生理生化特性指标下降,表现出明显的毒害作用。展开更多
为比较不同方式提取的蛇莓抽提物的活性差异,采用牛津杯法和微量肉汤二倍稀释法检测蛇莓抽提物的抑菌活性,以FRAP法、ABTS法、DPPH法、超氧阴离子自由基(O_(2)^(-)·)清除法评估抗氧化活性,通过测定NO和MDA含量、SOD活性、i NOSm RN...为比较不同方式提取的蛇莓抽提物的活性差异,采用牛津杯法和微量肉汤二倍稀释法检测蛇莓抽提物的抑菌活性,以FRAP法、ABTS法、DPPH法、超氧阴离子自由基(O_(2)^(-)·)清除法评估抗氧化活性,通过测定NO和MDA含量、SOD活性、i NOSm RNA表达变化评价蛇莓多糖抗脂质过氧化作用。结果显示,蛇莓多糖无抑菌活性,蛇莓醇提物的抑菌效果为7/11,蛇莓水提物的抑菌效果为5/11,蛇莓醇提物和蛇莓水提物均对化脓隐秘杆菌的生长呈高度抑制,最小抑菌浓度为0.98 mg/m L;蛇莓多糖的ABTS和DPPH自由基清除能力与Vc间无显著差异性(P>0.05),高于蛇莓醇提物和水提物差异极显著(P<0.01);蛇莓多糖能抑制LPS所诱导的RAW264.7细胞中产生NO和MDA、i NOS m RNA的表达(P<0.01),显著增强总SOD活力(P<0.01)。结果提示,蛇莓多糖虽然没有其醇提物和水提物的显著抑菌活性,但其抗氧化活性优于蛇莓醇提物和水提物,且能降低LPS诱导的巨噬细胞脂质过氧化反应损伤,蛇莓提取物有防治畜禽细菌感染性疾病前景。展开更多
文摘Duchesnea indica (Andr.) Focke has been traditionally used to treat cancer in Asian countries for centuries. In the present study, transplanted U14 cervical cancer mouse model was used to evaluate the antitumor and immunomodulatory activity of Duchesnea phenolic fraction (DPF). ELISA and RIA assay were employed to measured the serum concentration of Th1/Th2 cytokines (IL-2, IL-4, IFN-γ and TNF-α). Administration with 0.25 g/kg, 0.5 g/kg and 1 g/kg DPF significantly reduced the tumor weight by 34.37%, 43.89% and 56.28%, respectively, as compared to the tumor control group. Furthermore, the serum level of IL-2, IFN-γ and TNF-α increased and IL-4 level decreased in a dose-dependent manner during DPF treatment, indicating that the antitumor activity of DPF may be associated with the decrease of TNF-α level and restoration of the balance of Th1/Th2 cell responses. These data suggested that DPF, a mixture of plant polyphenols, had potent anticancer activity which was in part accomplished by its immunomodulatory ability.
文摘【目的】研究Cd污染对蛇泡草生理生化特性指标的影响,为重金属对中草药的毒害作用研究提供参考。【方法】采用盆栽模拟试验,待蛇泡草幼苗待生长稳定后,以50 m L/d的量浇灌不同浓度(11、22、45、90和134 mg/kg)的Cd2+溶液,记录蛇泡草5、30和45 d的生长情况,同时测定其的可溶性总糖含量、总黄酮含量、及抗氧化酶活性。【结果】随Cd溶液浓度的增加,蛇泡草体内Cd含量不断增大,同时蛇泡草叶片、茎部和根部的可溶性总糖、总黄酮、及抗氧化酶活性均呈现出不断降低的趋势。【结论】Cd较易在蛇泡草体内积累,并会破坏植物的新成代谢和正常生长,导致各项生理生化特性指标下降,表现出明显的毒害作用。
文摘为比较不同方式提取的蛇莓抽提物的活性差异,采用牛津杯法和微量肉汤二倍稀释法检测蛇莓抽提物的抑菌活性,以FRAP法、ABTS法、DPPH法、超氧阴离子自由基(O_(2)^(-)·)清除法评估抗氧化活性,通过测定NO和MDA含量、SOD活性、i NOSm RNA表达变化评价蛇莓多糖抗脂质过氧化作用。结果显示,蛇莓多糖无抑菌活性,蛇莓醇提物的抑菌效果为7/11,蛇莓水提物的抑菌效果为5/11,蛇莓醇提物和蛇莓水提物均对化脓隐秘杆菌的生长呈高度抑制,最小抑菌浓度为0.98 mg/m L;蛇莓多糖的ABTS和DPPH自由基清除能力与Vc间无显著差异性(P>0.05),高于蛇莓醇提物和水提物差异极显著(P<0.01);蛇莓多糖能抑制LPS所诱导的RAW264.7细胞中产生NO和MDA、i NOS m RNA的表达(P<0.01),显著增强总SOD活力(P<0.01)。结果提示,蛇莓多糖虽然没有其醇提物和水提物的显著抑菌活性,但其抗氧化活性优于蛇莓醇提物和水提物,且能降低LPS诱导的巨噬细胞脂质过氧化反应损伤,蛇莓提取物有防治畜禽细菌感染性疾病前景。
