BACKGROUND： Extracts of ginkgo biloba leaves （EGB） and its metabolites have been reported to enhance brain function and nerve behavior. It has also been hypothesized that they can protect neurons from oxidative str...BACKGROUND： Extracts of ginkgo biloba leaves （EGB） and its metabolites have been reported to enhance brain function and nerve behavior. It has also been hypothesized that they can protect neurons from oxidative stress. OBJECTIVE： To investigate protective effects of EGB on peroxide （H2O2）-induced oxidative stress damage in PC12 cells. DESIGN： Observational contrast study. SETTING： Department ofPathophysiology, Guangdong Pharmacological College. MATERIALS： EGB was provided by Xi＇an Fujie Biotechnological Development Company; 1640 culture medium, methylthiazolyl tetrazolium （MTT）, trypsin and dimathyl sulfoxide （DMSO） by Sigma Company; PC12 cell strain by Cell Center of Medical College of Zhongshan University; calf serum by Hangzhou Sijiqing Bioengineering Company; lactate dehydrogenase （LDH） kit by Nanjing Jiancheng Bioengineering Research Institute. METHODS： The experiment was carried out in Department of Cell Biology of Guangdong Pharmacological College from June to December 2005. ①Cell culture： PC12 cells were cultured in 1640 medium containing 200 g/L fetal calf serum. The cells were diluted to 1 × 10^7 L^-1 and washed every two days. Those cells were used to experiment until they grew in logarithm on solid wall. ② Grouping and intervention： PC12 cells （1 × 10^8L^-1） were plated in 96-well plates with the density of 200 μ L/hole and divided into three groups： normal control group （routinely adding media）, H2O2 group （treating with media and H2O2 for 20 hours） and EGB group （adding media, 100μmol/L EGB and 100 μmol/L H2O2）. ③ MTT assay： PC12 cells （1 × 10^8L^-1） were plated in 96-well plates and divided into three groups with 8 holes for each group. Under sterile condition, cells were added with 5 g/L MTT （100μL） and cultured for 4 hours. And then, 200 μ L DMSO fluid was added and shaken for 30 minutes until blue crystal products formed were dissolved soundly ④ Experimental evaluation： Absorbance （A） at 630 nm was measured and LDH activity was measured at the same time. MAIN OUTCOME MEASURES： Results of MTT assay and LDH activity. RESULTS： ① Results of MTT assay： A value was lower in the H2O2 group than that in the normal control group （P 〈 0.01）, while A value was higher in the EGB group than that in the H2O2 group （P 〈 0.01）. ② LDH activity： LDH activity was higher in the H2O2 group than that in the normal control group （P 〈 0.01 ）, while LDH activity was lower in the EGB group than that in the H2O2 group （P 〈 0.01）. CONCLUSION： EGB can inhibit H2O2-induced oxidative stress damage in PC12 cells possibly by preventing damage to the cell membrane.展开更多
Objective. To observe the effect of Ginkgo biloba extract injection （GB） in treating early diabetic nephropathy （DN）. Methods. Sixty DN patients were divided into two groups, the treated group were treated by GB a...Objective. To observe the effect of Ginkgo biloba extract injection （GB） in treating early diabetic nephropathy （DN）. Methods. Sixty DN patients were divided into two groups, the treated group were treated by GB and Western medicine, and the control group were given Western medicine alone. The study lasted for 4 weeks. Fasting plasma glucose （FPG）, blood pressure, 24 h urinary albumin excretion （UAE）, endogenous creatinine clearance rate （Ccr）, blood lipids and hemorheology indices were examined before and after the study. Results. Compared with the control group, UAE were significantly decreased （P〈0. 01） ; Ccr, blood lipids and hemorheology indices were all improved after treatment in the treated group （ P〈 0.05 or P〈0.01）. But in FPG and blood pressure there was no significant change between the treated group and the control group （P〉0.05）. Conclusion. GB is effective in treating early DN through decreasing urinary albumin excretion rate, regulating blood lipids, improving renal function and hemorheology.展开更多
Objective：To observe the protective effects of safflor Injection（SI） and extract of Ginkgo biloba（EGB） on lung ischemia-reperfusion injury（LIRI） and investigate its mechanism.Methods：In vivo rabbit model of LI...Objective：To observe the protective effects of safflor Injection（SI） and extract of Ginkgo biloba（EGB） on lung ischemia-reperfusion injury（LIRI） and investigate its mechanism.Methods：In vivo rabbit model of LIRI was reconstructed.Forty rabbits were randomly and equally divided into four groups：sham-operation group（sham group）,ischemia-reperfusion group（model group）,ischemia-reperfusion plus SI group（safflor group） and ischemia-reperfusion plus EGB injection group（EGB group）.Malondialdehyde（MDA） content,superoxide dismutase（SOD） and xanthine oxidase（XO） activity in serum were measured.The wet/dry weight ratio（W/D） of the lung tissue and activity of myeloperoxidase（MPO） were also tested.Ultrastructure change of the lung tissue was observed by the electron microscope.The expression of intercellular adhesion molecule-1（ICAM-1） was measured by immunohistochemistry（IHC）.Results：In the model group,MDA and XO increased and SOD decreased in serum compared with the sham group（P〈0.01）.The values of W/D,MPO and ICAM-1 of the model group were higher than those of the sham group（P〈0.01）,but those of the safflor group and EGB group were significantly lower than those of the model group（P〈0.01）.The IHC demonstrated that ICAM-1 expression in lung tissue of the model group was significantly higher than those of the safflor group（P〈0.01）.Compared with safflor group,in the EGB group MDA,XO,MPO decreased,SOD and ICAM-1expression increased（P〈0.05）,but the change of W/D was not statistically significant（P〉0.05）.Conclusions：SI and EGB may attenuate LIRI through antioxidation,inhibition of neutrophil aggregation and down-regulation of ICAM-1expression.But EGB had more effect on the antioxidation,while SI did better on regulating ICAM-1 expression.展开更多
目的探究银杏叶提取物注射液联合多奈哌齐对脑梗死后认知障碍患者血管内皮功能、氧化应激反应及认知水平的影响。方法选取收治的126例脑梗死后认知障碍患者为研究对象,用随机数字表法分为对照组和观察组,各63例。2组均行常规治疗,在此...目的探究银杏叶提取物注射液联合多奈哌齐对脑梗死后认知障碍患者血管内皮功能、氧化应激反应及认知水平的影响。方法选取收治的126例脑梗死后认知障碍患者为研究对象,用随机数字表法分为对照组和观察组,各63例。2组均行常规治疗,在此基础上对照组给予多奈哌齐治疗,观察组给予银杏叶提取物注射液联合多奈哌齐治疗,比较2组美国国立卫生研究院卒中量表(National Institutes of Health Stroke Scale,NIHSS)评分、血管内皮功能[内皮素(endothelin,ET)-1、一氧化氮]、氧化应激反应[干扰素-γ(interferon-γ,INF-γ)、超氧化物歧化酶(superoxide dismutase,SOD)和丙二醛(malondialdehyde,MDA)]和特利尔认知评估量表(Montreal cognitive assessment,MoCA)评分,以及不良反应的发生情况。结果治疗前,2组NIHSS评分比较差异无统计学意义(P>0.05),治疗后2组上述评分均下降,且观察组较对照组更低(P<0.05);治疗前2组血清ET-1、一氧化氮、INF-γ、SOD和MDA水平比较差异无统计学意义,治疗后2组ET-1、INF-γ、MDA水平均下降,一氧化氮、SOD水平均升高,且观察组均优于对照组(P<0.05);治疗前2组MoCA评分比较差异无统计学意义(P>0.05),治疗后2组上述评分均升高,且观察组较对照组更高(P<0.05);2组不良反应总发生率比较差异无统计学意义(P>0.05)。结论脑梗死后认知障碍患者应用银杏叶提取物注射液联合多奈哌齐治疗,可有效改善神经功能、血管内皮功能,拮抗氧化应激水平,从而提高认知水平,且安全性良好。展开更多
基金Initial Funding for Doctor from Guangdong Science and Technology Bureau, No. 06300709Initial Funding for Doctor from Guangdong Pharmaceutical College, No. 43543096
文摘BACKGROUND： Extracts of ginkgo biloba leaves （EGB） and its metabolites have been reported to enhance brain function and nerve behavior. It has also been hypothesized that they can protect neurons from oxidative stress. OBJECTIVE： To investigate protective effects of EGB on peroxide （H2O2）-induced oxidative stress damage in PC12 cells. DESIGN： Observational contrast study. SETTING： Department ofPathophysiology, Guangdong Pharmacological College. MATERIALS： EGB was provided by Xi＇an Fujie Biotechnological Development Company; 1640 culture medium, methylthiazolyl tetrazolium （MTT）, trypsin and dimathyl sulfoxide （DMSO） by Sigma Company; PC12 cell strain by Cell Center of Medical College of Zhongshan University; calf serum by Hangzhou Sijiqing Bioengineering Company; lactate dehydrogenase （LDH） kit by Nanjing Jiancheng Bioengineering Research Institute. METHODS： The experiment was carried out in Department of Cell Biology of Guangdong Pharmacological College from June to December 2005. ①Cell culture： PC12 cells were cultured in 1640 medium containing 200 g/L fetal calf serum. The cells were diluted to 1 × 10^7 L^-1 and washed every two days. Those cells were used to experiment until they grew in logarithm on solid wall. ② Grouping and intervention： PC12 cells （1 × 10^8L^-1） were plated in 96-well plates with the density of 200 μ L/hole and divided into three groups： normal control group （routinely adding media）, H2O2 group （treating with media and H2O2 for 20 hours） and EGB group （adding media, 100μmol/L EGB and 100 μmol/L H2O2）. ③ MTT assay： PC12 cells （1 × 10^8L^-1） were plated in 96-well plates and divided into three groups with 8 holes for each group. Under sterile condition, cells were added with 5 g/L MTT （100μL） and cultured for 4 hours. And then, 200 μ L DMSO fluid was added and shaken for 30 minutes until blue crystal products formed were dissolved soundly ④ Experimental evaluation： Absorbance （A） at 630 nm was measured and LDH activity was measured at the same time. MAIN OUTCOME MEASURES： Results of MTT assay and LDH activity. RESULTS： ① Results of MTT assay： A value was lower in the H2O2 group than that in the normal control group （P 〈 0.01）, while A value was higher in the EGB group than that in the H2O2 group （P 〈 0.01）. ② LDH activity： LDH activity was higher in the H2O2 group than that in the normal control group （P 〈 0.01 ）, while LDH activity was lower in the EGB group than that in the H2O2 group （P 〈 0.01）. CONCLUSION： EGB can inhibit H2O2-induced oxidative stress damage in PC12 cells possibly by preventing damage to the cell membrane.
文摘Objective. To observe the effect of Ginkgo biloba extract injection （GB） in treating early diabetic nephropathy （DN）. Methods. Sixty DN patients were divided into two groups, the treated group were treated by GB and Western medicine, and the control group were given Western medicine alone. The study lasted for 4 weeks. Fasting plasma glucose （FPG）, blood pressure, 24 h urinary albumin excretion （UAE）, endogenous creatinine clearance rate （Ccr）, blood lipids and hemorheology indices were examined before and after the study. Results. Compared with the control group, UAE were significantly decreased （P〈0. 01） ; Ccr, blood lipids and hemorheology indices were all improved after treatment in the treated group （ P〈 0.05 or P〈0.01）. But in FPG and blood pressure there was no significant change between the treated group and the control group （P〉0.05）. Conclusion. GB is effective in treating early DN through decreasing urinary albumin excretion rate, regulating blood lipids, improving renal function and hemorheology.
文摘Objective：To observe the protective effects of safflor Injection（SI） and extract of Ginkgo biloba（EGB） on lung ischemia-reperfusion injury（LIRI） and investigate its mechanism.Methods：In vivo rabbit model of LIRI was reconstructed.Forty rabbits were randomly and equally divided into four groups：sham-operation group（sham group）,ischemia-reperfusion group（model group）,ischemia-reperfusion plus SI group（safflor group） and ischemia-reperfusion plus EGB injection group（EGB group）.Malondialdehyde（MDA） content,superoxide dismutase（SOD） and xanthine oxidase（XO） activity in serum were measured.The wet/dry weight ratio（W/D） of the lung tissue and activity of myeloperoxidase（MPO） were also tested.Ultrastructure change of the lung tissue was observed by the electron microscope.The expression of intercellular adhesion molecule-1（ICAM-1） was measured by immunohistochemistry（IHC）.Results：In the model group,MDA and XO increased and SOD decreased in serum compared with the sham group（P〈0.01）.The values of W/D,MPO and ICAM-1 of the model group were higher than those of the sham group（P〈0.01）,but those of the safflor group and EGB group were significantly lower than those of the model group（P〈0.01）.The IHC demonstrated that ICAM-1 expression in lung tissue of the model group was significantly higher than those of the safflor group（P〈0.01）.Compared with safflor group,in the EGB group MDA,XO,MPO decreased,SOD and ICAM-1expression increased（P〈0.05）,but the change of W/D was not statistically significant（P〉0.05）.Conclusions：SI and EGB may attenuate LIRI through antioxidation,inhibition of neutrophil aggregation and down-regulation of ICAM-1expression.But EGB had more effect on the antioxidation,while SI did better on regulating ICAM-1 expression.
文摘目的探究银杏叶提取物注射液联合多奈哌齐对脑梗死后认知障碍患者血管内皮功能、氧化应激反应及认知水平的影响。方法选取收治的126例脑梗死后认知障碍患者为研究对象,用随机数字表法分为对照组和观察组,各63例。2组均行常规治疗,在此基础上对照组给予多奈哌齐治疗,观察组给予银杏叶提取物注射液联合多奈哌齐治疗,比较2组美国国立卫生研究院卒中量表(National Institutes of Health Stroke Scale,NIHSS)评分、血管内皮功能[内皮素(endothelin,ET)-1、一氧化氮]、氧化应激反应[干扰素-γ(interferon-γ,INF-γ)、超氧化物歧化酶(superoxide dismutase,SOD)和丙二醛(malondialdehyde,MDA)]和特利尔认知评估量表(Montreal cognitive assessment,MoCA)评分,以及不良反应的发生情况。结果治疗前,2组NIHSS评分比较差异无统计学意义(P>0.05),治疗后2组上述评分均下降,且观察组较对照组更低(P<0.05);治疗前2组血清ET-1、一氧化氮、INF-γ、SOD和MDA水平比较差异无统计学意义,治疗后2组ET-1、INF-γ、MDA水平均下降,一氧化氮、SOD水平均升高,且观察组均优于对照组(P<0.05);治疗前2组MoCA评分比较差异无统计学意义(P>0.05),治疗后2组上述评分均升高,且观察组较对照组更高(P<0.05);2组不良反应总发生率比较差异无统计学意义(P>0.05)。结论脑梗死后认知障碍患者应用银杏叶提取物注射液联合多奈哌齐治疗,可有效改善神经功能、血管内皮功能,拮抗氧化应激水平,从而提高认知水平,且安全性良好。
