Objective:To study the serum expression and clinical significance of miR-342-5p and miR-423-5p in type 2 diabetes mellitus patients with different urinary albumin excretion rate. Methods: A total of 126 patients with ...Objective:To study the serum expression and clinical significance of miR-342-5p and miR-423-5p in type 2 diabetes mellitus patients with different urinary albumin excretion rate. Methods: A total of 126 patients with type 2 diabetes mellitus treated in our hospital between May 2013 and December 2015 were selected and divided into normal urine protein group (NA group), micro-urine protein group (MI group) and macro-urine protein group (MA group) according to the urinary albumin/creatinine ratio (ACR);50 healthy volunteers were selected as control group. Serum was collected to determine the expression miR-342-5p and miR-423-5p as well as the content of p38MAPK and TGF-毬1 signaling pathway molecules.Results:Serum miR-342-5p and miR-423-5p expression of NA group, MI group and MA group were significantly higher than those of control group and the higher the ACR, the higher the serum miR-342-5p and miR-423-5p expression;serum MAPKKK, p38MAPK, CREB, ATF-2, TGF-毬1, Smad2, Smad3, Smad4 and CTGF content of patients with high miR-342-5p and miR-423-5p expression were significantly higher than those of patients with low miR-342-5p and miR-423-5p expression.Conclusions: Highly expressed miR-342-5p and miR-423-5p in patients with type 2 diabetes mellitus can be targeted to adjust p38MAPK and TGF-毬1 signaling pathway to cause the occurrence of diabetic nephropathy.展开更多
文摘Objective:To study the serum expression and clinical significance of miR-342-5p and miR-423-5p in type 2 diabetes mellitus patients with different urinary albumin excretion rate. Methods: A total of 126 patients with type 2 diabetes mellitus treated in our hospital between May 2013 and December 2015 were selected and divided into normal urine protein group (NA group), micro-urine protein group (MI group) and macro-urine protein group (MA group) according to the urinary albumin/creatinine ratio (ACR);50 healthy volunteers were selected as control group. Serum was collected to determine the expression miR-342-5p and miR-423-5p as well as the content of p38MAPK and TGF-毬1 signaling pathway molecules.Results:Serum miR-342-5p and miR-423-5p expression of NA group, MI group and MA group were significantly higher than those of control group and the higher the ACR, the higher the serum miR-342-5p and miR-423-5p expression;serum MAPKKK, p38MAPK, CREB, ATF-2, TGF-毬1, Smad2, Smad3, Smad4 and CTGF content of patients with high miR-342-5p and miR-423-5p expression were significantly higher than those of patients with low miR-342-5p and miR-423-5p expression.Conclusions: Highly expressed miR-342-5p and miR-423-5p in patients with type 2 diabetes mellitus can be targeted to adjust p38MAPK and TGF-毬1 signaling pathway to cause the occurrence of diabetic nephropathy.
