In Xenopus, injection of S-adenosylmethionine decarboxylase (SAMDC) mRNA into fertilized eggs or 2-cell stage embryos induces massive cell dissociation and embryo-lysis at the early gastrula stage due toactivation of ...In Xenopus, injection of S-adenosylmethionine decarboxylase (SAMDC) mRNA into fertilized eggs or 2-cell stage embryos induces massive cell dissociation and embryo-lysis at the early gastrula stage due toactivation of the maternal program of apoptosis. We injected SAMDC mRNA into only one of the animalside blastomeres of embryos at different stages of cleavage, and examined the timing of the onset of theapoptotic reaction. In the injection at 4-and 8-cell stages, a considerable number of embryos developed intotadpoles and in the injection at 16-and 32-cell stages, all the embryos became tadpoles, although tadpolesobtained were sometimes abnormal. However, using GFP as a lineage tracer, we found that descendant cellsof the blastomere injected with SAMDC mRNA at 8-to 32-cell stages are confined within the blastocoel atthe early gastrula stage and undergo apoptotic cell death within the blastocoel, in spite of the continued development of the injected embryos. These results indicate that cells overexpressed with SAMDC undergo apoptotic cell death consistently at the early gastrula stage, irrespective of the timing of the mRNA injection.We assume that apoptosis is executed in Xenopus early gastrulae as a 'fall-safe' mechanism to eliminate physiologically-severely damaged cells to save the rest of the embryo.展开更多
New models of safety-critical systems are built here. In these systems, when components fail, different defect states have different effects, hence need different ways to measure. In the models, there are two kinds of...New models of safety-critical systems are built here. In these systems, when components fail, different defect states have different effects, hence need different ways to measure. In the models, there are two kinds of failure modes of the components： one could be called failed-safe, and the other may be named failed- dangerous In practice, the so-called failed-dangerous components may lead a system to peril. However, failed-safe components will not. Reliability and safety issues are analyzed using Ion-Channel modeling theory to get count of repairs and time duration before the system becomes dangerous. In the closing section a numerical example is presented to illustrate the results obtained in the paper.展开更多
Quantitative safety assessment of safety systems plays an important role in decision making at all stages of system lifecycle, i.e., design, deployment and phase out. Most safety assessment methods consider only syste...Quantitative safety assessment of safety systems plays an important role in decision making at all stages of system lifecycle, i.e., design, deployment and phase out. Most safety assessment methods consider only system parameters, such as configuration, hazard rate, coverage, repair rate, etc. along with periodic proof-tests （or inspection）. Not considering demand rate will give a pessimistic safety estimate for an application with low demand rate such as nuclear power plants, chemical plants, etc. In this paper, a basic model of IEC 61508 is used. The basic model is extended to incorporate process demand and behavior of electronic- and/or computer-based system following diagnosis or proof-test. A new safety index, probability of failure on actual demand （PFAD） based on extended model and demand rate is proposed. Periodic proof-test makes the model semi-Markovian, so a piece-wise continuous time Markov chain （CTMC） based method is used to derive mean state probabilities of elementary or aggregated state. Method to determine probability of failure on demand （PFD） （IEC 61508） and PFAD based on these state probabilities are described. In example, safety indices of PFD and PFAD are compared.展开更多
传统结构由于缺少冗余,忽略了不确定性因素的影响,更容易受到局部刚度损失的影响,文章针对载荷不确定性下破损-安全结构的设计问题提出了一种有效的基于响应面的可靠性拓扑优化方法,以提高结构的安全性,确保结构在发生局部破损时仍能满...传统结构由于缺少冗余,忽略了不确定性因素的影响,更容易受到局部刚度损失的影响,文章针对载荷不确定性下破损-安全结构的设计问题提出了一种有效的基于响应面的可靠性拓扑优化方法,以提高结构的安全性,确保结构在发生局部破损时仍能满足服役性能及可靠性要求.为此,建立了柔度概率约束下的结构体积比最小化的双循环可靠性拓扑优化模型,其中内层循环实施可靠性分析,外层循环实施拓扑优化.为了有效处理可靠性分析中响应函数关于随机变量的导数计算高成本问题,基于响应面方法建立了响应函数关于随机变量的显式表达式.详细推导了响应函数关于设计变量和随机变量的解析灵敏度列式,并采用移动渐近线方法(method of moving asymptotes,MMA)对优化问题进行求解.将基于响应面的可靠性拓扑优化方法与基于解析导数的方法作对比,并实施蒙特卡洛仿真验证了所提方法的有效性和优越性,讨论了随机载荷标准差对优化结果的影响.结果表明,本文方法可以有效设计满足指定可靠性水平的破损-安全结构,优化后结构可靠性指标的相对误差不超过1.3%,另外基于响应面的可靠性设计方法相对于基于解析导数的可靠性设计方法可节省约74%的可靠性分析时间.展开更多
文摘In Xenopus, injection of S-adenosylmethionine decarboxylase (SAMDC) mRNA into fertilized eggs or 2-cell stage embryos induces massive cell dissociation and embryo-lysis at the early gastrula stage due toactivation of the maternal program of apoptosis. We injected SAMDC mRNA into only one of the animalside blastomeres of embryos at different stages of cleavage, and examined the timing of the onset of theapoptotic reaction. In the injection at 4-and 8-cell stages, a considerable number of embryos developed intotadpoles and in the injection at 16-and 32-cell stages, all the embryos became tadpoles, although tadpolesobtained were sometimes abnormal. However, using GFP as a lineage tracer, we found that descendant cellsof the blastomere injected with SAMDC mRNA at 8-to 32-cell stages are confined within the blastocoel atthe early gastrula stage and undergo apoptotic cell death within the blastocoel, in spite of the continued development of the injected embryos. These results indicate that cells overexpressed with SAMDC undergo apoptotic cell death consistently at the early gastrula stage, irrespective of the timing of the mRNA injection.We assume that apoptosis is executed in Xenopus early gastrulae as a 'fall-safe' mechanism to eliminate physiologically-severely damaged cells to save the rest of the embryo.
基金Sponsored by 211 Project of Minzu University of China(021211030312)
文摘New models of safety-critical systems are built here. In these systems, when components fail, different defect states have different effects, hence need different ways to measure. In the models, there are two kinds of failure modes of the components： one could be called failed-safe, and the other may be named failed- dangerous In practice, the so-called failed-dangerous components may lead a system to peril. However, failed-safe components will not. Reliability and safety issues are analyzed using Ion-Channel modeling theory to get count of repairs and time duration before the system becomes dangerous. In the closing section a numerical example is presented to illustrate the results obtained in the paper.
文摘Quantitative safety assessment of safety systems plays an important role in decision making at all stages of system lifecycle, i.e., design, deployment and phase out. Most safety assessment methods consider only system parameters, such as configuration, hazard rate, coverage, repair rate, etc. along with periodic proof-tests （or inspection）. Not considering demand rate will give a pessimistic safety estimate for an application with low demand rate such as nuclear power plants, chemical plants, etc. In this paper, a basic model of IEC 61508 is used. The basic model is extended to incorporate process demand and behavior of electronic- and/or computer-based system following diagnosis or proof-test. A new safety index, probability of failure on actual demand （PFAD） based on extended model and demand rate is proposed. Periodic proof-test makes the model semi-Markovian, so a piece-wise continuous time Markov chain （CTMC） based method is used to derive mean state probabilities of elementary or aggregated state. Method to determine probability of failure on demand （PFD） （IEC 61508） and PFAD based on these state probabilities are described. In example, safety indices of PFD and PFAD are compared.
文摘传统结构由于缺少冗余,忽略了不确定性因素的影响,更容易受到局部刚度损失的影响,文章针对载荷不确定性下破损-安全结构的设计问题提出了一种有效的基于响应面的可靠性拓扑优化方法,以提高结构的安全性,确保结构在发生局部破损时仍能满足服役性能及可靠性要求.为此,建立了柔度概率约束下的结构体积比最小化的双循环可靠性拓扑优化模型,其中内层循环实施可靠性分析,外层循环实施拓扑优化.为了有效处理可靠性分析中响应函数关于随机变量的导数计算高成本问题,基于响应面方法建立了响应函数关于随机变量的显式表达式.详细推导了响应函数关于设计变量和随机变量的解析灵敏度列式,并采用移动渐近线方法(method of moving asymptotes,MMA)对优化问题进行求解.将基于响应面的可靠性拓扑优化方法与基于解析导数的方法作对比,并实施蒙特卡洛仿真验证了所提方法的有效性和优越性,讨论了随机载荷标准差对优化结果的影响.结果表明,本文方法可以有效设计满足指定可靠性水平的破损-安全结构,优化后结构可靠性指标的相对误差不超过1.3%,另外基于响应面的可靠性设计方法相对于基于解析导数的可靠性设计方法可节省约74%的可靠性分析时间.
