Use of (Dig)-DNA Probe for the Epidemiological Survey of Plasmodium falciparum Malaria

The Plasmodtum falctparum DNA fragment was isolated from a cloned recombinant plasmid pPF14. labelled with Digoxigenin (Dig) and used as a probe (pPF14-F-Dig)to detect 274 blood samples from the eqdemic area population in Hainan Province by dot hybridization.The results showed that out of 274 blood specimens one was positive when using microscopic examination and the positivity rate was 0.36 percent. Fifteen samples showed to be positive (including one positive specimen examined by microscopy) when this probe was applied to detect the 274 samples and its positivity rate was 5.47 percent.The positive coincidence rate between pPF14-F-Dig and microscopic examination was 1/1 and the negative coincidence rate was 94.87 percent. Since a piece of nitrocellulose membrane with the size of 9 by 12 square centimetres can accommodate blots of 96 samples,this probe is fit for large-scale epidemiological surveys.

中西医结合治疗乙型慢性活动性肝炎的疗效观察

对４１例乙型慢性活动性肝炎（ＣＡＨ）患者随机分为二组，一组（简称治疗组）（２１例）口服活血化瘀中药，同时静脉滴注强力宁注射液８０～１００ｍｌ／天，疗程３个月；另一组（简称对照组）（２０例）为常规用一般护肝药，疗程３个月。结果中西医结合治疗组降ＧＰＴ有效率为８６％，对照组为７４％ ；中西医结合组ＨＢｅＡｇ阴转率为５５％，抗ＨＢｅ阳转率为３７．５％，对照组分别为１６．６％、８．３％（Ｐ＜０．０５）。结果提示，中西医结合治疗ＣＡＨ效果较好。