A Study of the Treatment of Disfiguring Diseases in the “Qian Jin Fang”

Traditional Chinese medicine(TCM)has been passed down for more than 2,000 years.TCM beauty products driven by the principles of identification and treatment with various dosage forms and exhibit mild and safe efficacy are bound to become a main component of the beauty industry in the future.Xu once commented that“Qian Jin Fang”is different from the traditional prescription system,which emphasizes the use of medicines.In this paper,we mainly selected the prescriptions for the treatment of disfigurement diseases such as acne,scarring,black dryness,face hyperpigmentation,black mole,and rosacea from the article titled,“Upper Seven Orifices Disease-Facial Medicine IX”in the Bei Ji Qian Jin Yao Fang[1].By studying and analyzing the original composition of the formula,understanding the etiology and pathogenesis of the disease,and exploring the characteristics of the formula concerning the efficacy of TCM,their properties,flavors,and meridians,we summarized the characteristics of the Qian Jin Fang.It is based on the external treatment of disfigurement diseases,with a wide variety of drugs,flexibility,and accuracy.This method draws upon analogies while utilizing excipients and harmonizers to focus on the combined healing approach of treatment and nourishment.

Clinical Study on the Effectiveness of Xian Fang Huo Ming Yin for Treating Cutaneous Infections and Promoting Wound Healing in Patients with Perianal Abscess

Objective:To explore the effect of the Xian Fang Huo Ming Yin(XFHM)for treating cutaneous infections and promoting wound healing in patients with perianal abscesses.Methods:Sixty-one patients with perianal abscesses who were admitted to our hospital(Xinghua City People’s Hospital)from May 2022 to May 2023 were selected and randomly divided into two groups,a control group(30 cases)and a study group(31 cases).Both groups received surgical treatment.The control group received conventional treatment and warm water fumigation,sitz bath,and surgical dressing change after surgery,while the research group received XFHM based on the control group.XFHM was taken orally and replaced with warm water for fumigation and sitz bathing.Both groups received treatment for 4 weeks but discontinued sitz bathing after 2 weeks.Various clinical indicators between the two groups were compared.Results:The total clinical effective rate and wound recovery rate of the study group were higher than that of the control group.There were differences in the wound pain scores,surrounding tissue edema,and wound secretions at different time points.Both groups experienced wound pain.The scores of wound pain,surrounding tissue edema,and wound secretions of the study group were lower than those of the control group,7 and 14 days after surgery.The serum interleukin 6(IL-6),tumor necrosis factor-alpha(TNF-α)levels,and pH values of the study group were lower than those of the control group 10 days after surgery(P<0.05).Conclusion:The application of XFHM for treating cutaneous infections and promoting wound healing in patients with perianal abscesses improved the treatment outcome,alleviated clinical symptoms,and promoted healing.

Possible mechanisms associated with immune escape and apoptosis on anti-hepatocellular carcinoma effect of Mu Ji Fang granules

BACKGROUND Hepatocellular carcinoma(HCC)is one of the most common digestive system cancers with high mortality rates worldwide.The main ingredients in Mu Ji Fang Granules(MJF)are alkaloids,flavonoids,and polysaccharides.MJF has been used in the clinical treatment of hepatitis,cirrhosis and HCC for more than 30 years.Few previous studies have focused on the mechanism of MJF on tumor immunology in the treatment of HCC.AIM To explore the mechanism of action of MJF on tumor immunology in the treatment of HCC.METHODS The absorbable ingredients of MJF were identified using Molecule Network related to High Performance Liquid Chromatography-Electron Spray Ionization-Time of Flight-Mass Spectrometry,and hub potential anti-HCC targets were screened using network pharmacology and pathway enrichment analysis.Forty male mice were randomly divided into the Blank,Model,and MJF groups(1.8,5.4,and 10.8 g/kg/d)following 7 d of oral administration.Average body weight gain,spleen and thymus indices were calculated,tumor tissues were stained with hematoxylin and eosin,and Interferon gamma(IFN-γ),Tumor necrosis factorα(TNF-α),Interleukin-2,aspartate aminotransferase,alanine aminotransferase,alpha-fetoprotein(AFP),Fas,and FasL were measured by Enzyme-linked Immunosorbent Assay.Relevant mRNA expression of Bax and Bcl2 was evaluated by Real Time Quantitative PCR(RTqPCR)and protein expression of Transforming growth factorβ1(TGF-β1)and Mothers against decapentaplegic homolog(SMAD)4 was assessed by Western blotting.The HepG2 cell line was treated with 10 mg/mL,20 mg/mL,30 mg/mL,40 mg/mL of MJF,and another 3 groups were treated with TGF-β1 inhibitor(LY364947)and different doses of MJF.Relevant mRNA expression of TNF-α,IFN-γ,Bax and Bcl2 was evaluated by RT-qPCR and protein expression of TGF-β1,SMAD2,p-SMAD2,SMAD4,and SMAD7 was assessed by Western blotting.RESULTS It was shown that MJF improved body weight gain and tumor inhibition rate in H22 tumorbearing mice,protected immune organs and liver function,reduced the HCC indicator AFP,affected immunity and apoptosis,and up-regulated the TGF-β1/SMAD signaling pathway,by increasing the relative expression of TGF-β1,SMAD2,p-SMAD2 and SMAD4 and decreasing SMAD7,reducing immune factors TNF-αand IFN-γ,decreasing apoptosis cytokines Fas,FasL and Bcl2/Bax,and inhibiting the effect of LY364947 in HepG2 cells.CONCLUSION MJF inhibits HCC by activating the TGF-β1/SMAD signaling pathway,and affecting immune and apoptotic cytokines,which may be due to MJF adjusting immune escape and apoptosis.

Mechanism of Huatan Sanjie Fang in improving goiter in Graves'disease mice based on the Hippo signaling pathway

Objective:To explore the mechanism of Huatan Sanjie Fang(HTSJ)in regulating goiter in Graves'disease(GD)mice by detecting key factors of the Hippo signaling pathway.Methods:A mouse model of GD was established by injecting Ad-TSHR289 adenovirus into the bilateral quadriceps femoris of female mice.Successful mouse models were then randomly divided into a model group,methimazole(MMI)group,and HTSJ group,and fed with deionized water,MMI(4.5 mg/kg per day),and HTSJ(35.10 g/kg per day),respectively,for 10 weeks.Histopathological changes of the thyroid gland were subsequently observed by hematoxylin-eosin staining.Radioimmunoassay was used to detect serum total thyroxine(T4)and thyrotrophin-receptor antibody(TRAb)levels.The relative expression of mRNA of Mst1,YAP,and TAZ were detected by quantitative real-time polymerase chain reaction,while the protein expression of Mst1,YAP,TAZ,pMst1,and pYAP were detected by western blot.Results:After 10 weeks of drug intervention,goiter and other pathological changes in the HTSJ group significantly improved compared with the model group,and the levels of serum T4 and TRAb significantly decreased(P=.002,P<.001,respectively).Decreased mRNA expression of Mst1,YAP,and TAZ,the key factors of the Hippo signaling transduction pathway,was also observed(P=.002,P=.022,P<.001,respectively).In contrast,protein expression of Mst1(P=.046),pMst1(P=.026),and p YAP(P=.004)increased,while protein expression of YAP and TAZ decreased(P=.041,P<.001,respectively).Conclusion:HTSJ can effectively improve goiter in GD mice through the Hippo signaling pathway.

Regulations for the Manufacture and Control of Live Poliovirus Vaccine: International Experience and China’s Path

The eradication of poliomyelitis is a landmark achievement in the history of public health, providing strong protection for children’s health. The introduction of the Chinese Regulations for the Manufacture and Control of Live Poliovirus Vaccine is a prerequisite and safeguard for the large-scale production and use of domestically produced live poliovirus vaccines, serving as an indispensable component of vaccine safety. This article, based on archival documents, letters, collections of essays, and oral interviews, examines the historical experience of the development of Chinese Regulations for the Manufacture and Control of Live Poliovirus Vaccine. It contends that the emphasis on localization and the active engagement in international cooperation are critical factors in the swift introduction of Chinese Regulations for the Manufacture and Control of Live Poliovirus Vaccine.

张氏的年龄结构及生长特性

以鳞片作为年龄鉴定材料,对长江上游特有鱼类张氏（Hemiculter tchangi Fang）种群的年龄结构和生长特性进行了研究。结果表明：张氏鳞片的年轮结构呈疏密切割型,2-3月是年轮形成最多的月份。其渔获物由1-4龄组成,其中857尾为1-2龄,占总数的83.84%;雌∶雄性比为1.07∶1,符合1∶1理论比值（X^2=0.754,P〉0.05,n=1001）,随着年龄的增长呈雌鱼多于雄鱼的趋势。体长主要分布在81-180 mm（94.9%）,体重主要分布在2.9-50.0 g（67.69%）。体长和鳞径呈直线关系,体长和体重呈幂函数关系且幂指数接近3,属于匀速生长类型,生长方程为Lt=196.05（1-e^-0.36（t＋1.26）;Wt=97.195（1-e^-0.36（t＋1.26）^3.145,拐点年龄为1.92龄,相应的体长体重分别为133.5 mm、29.13 g。

Mechanism of anti-arterial thrombosis of Dahuangzhecong Fang screened by Ti-Al intermetallic compound porous material

The mechanism of antithrombotic of Dahuangzhechong Fang separated and purified by Ti-Al intermetallic compound porous material (TAICPM) was researched. Dahuangzhechong Fang, which was isolated and screened by TAICPM, was used to oral rats. At the end of study, their blood and thrombus were collected. The results show that TAICPM with the pore size of 1-5 μm can screen Dahuangzhechong Fang well. Dahuangzhechong Fang can increase 6-keto-PGF1α, lower content of TXD2 and platelet. Dahuangzhechong Fang has good effect to resist arterial thrombosis.

南水水库鲂鱼体长与鳞长的生长关系

研究表明 :鲂鱼的体长与鳞长的生长呈显著的正线性相关 .L =1.432 2 +4 5 .749R ,r=0 .95 31,n =187.从生长指标看 ,属均匀生长型 ,6龄前增长较快 ,6龄后生长减慢 ,其中

基于超宽带技术的TDOA室内三维定位算法研究

在室内环境下对目标进行无线定位时,由于障碍物的遮挡而造成的非视距(NLOS)误差对定位精度产生了很大的影响。针对此问题,对利用超宽带(UWB)技术测量得到的到达时间差(TDOA)数据进行残差分析,首先鉴别测得的数据中是否存在NLOS误差,然后针对存在NLOS误差的情况,提出将Fang算法得到的定位结果作为泰勒级数展开法的初始定位值,组成Fang-Taylor级数联合算法来计算NLOS情况下的定位结果。而对于视距(LOS)情况下测得的数据,仍采用单一的Fang算法进行计算。仿真对比实验表明,Fang-Taylor级数联合算法有效地提高了室内NLOS环境下目标的定位精度。

基于Fang算法的TDOA室内定位技术

传统基于全球定位系统(GPS)的定位技术应用,已在室外环境下为人们提供了许多便利。随着近年来人们生活水平的不断提高,大众对定位的诉求已不仅限于室外,在智慧商场、企业人员智能管理、校园智能管理等场景,要求对室内用户进行定位监测,而传统的GPS定位精确度有限,在室内已无法应用。目前室内定位常用的技术有红外线、蓝牙、Wi-Fi以及基于室内移动网路的无线定位等,无线定位则是其中的热点。本文介绍了一种基于室内分布式基站,运用Fang算法实现到达时间差(TDOA)定位的技术研究,并对其定位精确度进行了探索。

李昉的类书编纂思想及成就

在宋太宗时两度入相的李 ,史籍只注重他作为循吏为人厚道的一面却忽视了他在文献整理上的成就。他主编了《太平广记》、《太平御览》和《文苑英华》三大部类书 ,在编纂方法上多所创新 ,为保存中国古典文献贡献巨大。尤其是他开创性地主持编纂野史笔记类书《太平广记》 ,体现了他宽广的文化视野。

历代对任日方骈文之评价

六朝的文学观念 ,无论诗赋乐府还是实用型文章都属于“文”,都要讲究与文体要求相符的语言美 ,任日方的骈文即代表着梁代实用型骈体文的最高水平。由于文学观念的差异 ,历代对任日方骈文的评价分歧较多 ,主要集中于用典特点及其有无逸气两方面。明清时期有多家评论 ,但大多为评点式的 ,难免以偏概全。至现代学者刘师培、骆鸿凯 ,才较为全面、深刻和细致地分析和概括了任日方骈文的艺术特色与语言魅力。

鲍叔鼎《医方约说》初探

《医方约说》为明·鲍叔鼎编撰,现孤本存于上海中医药大学。文章通过对《医方约说》文献整理研究,考证作者学术渊源和《医方约说》版本状况,认为《医方约说》集张仲景、李东垣、刘完素、朱丹溪"四子"之说,每论能勾摘各家之长,而以丹溪为宗,每篇总论之后多列主方,后随脉证加减用药,次列附方,使读者便于据证检方用药。通过本书的整理研究,认为本书为丹溪学派著作,宣扬丹溪学派学术观点,列方效验便廉,达到为初学者引径入门,为病家据证用药的目的。

New Replacement Name for Rana paradoxa Mocquard,1890 with Designations of Lectotypes for Rana paradoxa and Rana conspicillata Günther,1872:Both Synonymized with Limnonectes kuhlii (Tschudi,1838) (Dicroglossidae:Dicroglossinae)

Fanged frogs, now called Limnonectes kuhlii, from Borneo are remotely related to true Javanese L. kuhliL For future taxonomy of Bornean fanged frogs, we fix the nomenclatural status of two existing names, Rana conspicillata Giunther, 1872 and Rana paradoxa Mocquard, 1890. Morphological comparison of the type-series revealed heterospecific relationships of the two species. For R. conspicillata, we designate BMNH 1947.2.29.20 as the lectotype, and for R. para doxa, we also designate MNHN 1889.222 as the lectotype and propose a replacement name Limnonectes mocquardi, in order to stabilize their nomenclature.

基于聚类和TDOA的无线基站定位算法研究

为提高无线定位的精度,提出了基于聚类和TDOA的基站定位优化算法。首先,建立TDOA定位模型,采用Fang算法对移动终端的位置进行初步估计,并根据多个初步估计值获得的先验信息,去除双解情况下误差较大的估计位置;其次,采用K-means聚类算法进行聚类,进一步筛选出离群点,得到更精确的位置估计数据集,将其取平均后得到精确位置;最后,采用仿真实验验证算法的性能。实验结果表明,相对于经典Fang算法,所提算法具有更优的定位性能,应用前景广阔。

乳腺癌抑郁中医证候系统综述

[背景]乳腺癌患者抑郁程度较高,严重影响生活质量。[目的]寻找乳腺癌中医证候规律。[检索策略]检索词：乳腺癌、抑郁、辨证、经验、证型、证候,检索CNKI、Wan Fang Data、VIP建库至2014年10月,中医证型研究/中医辨证分型特点/专方治疗特点/基本症状描述相关文献,同时追溯纳入文献/参考文献。[纳入标准]1研究类型：对照研究、经验报道、临床研究。文种限中文。2研究对象：种族、国籍、病程不限;排除其他疾病、证候或合并症。3资料完整。具有中医证型研究或中医辨证分型特点或专方治疗特点或基本症状描述。[资料提取与分析]手工检索：双人独立完成,并核对检索结果,意见不统一,咨询2~3名专家讨论决定,保证纳入文献符合标准。采用Excel2013建立数据库,求合、排序。[结果]初检88篇中文文献,阅读文献题名、摘要及全文,排除82篇,纳入6篇,均为中文文献。乳腺癌抑郁状态为虚证,肝肾阴虚为主;常见单一证候单证型：气滞证最为常见;病位在肝,与心,脾,肾关系密切。[结论]乳腺癌抑郁状态以肝肾阴虚、肝郁脾虚、肝郁气滞为主。[问题与展望]目前对乳腺癌抑郁状态的中医证候研究尚属初始阶段,今后有必要开展多中心大样本的相关中医证候调查。

Tong xie yao fang relieves irritable bowel syndrome in rats via mechanisms involving regulation of 5-hydroxytryptamine and substance P

AIM: To investigate whether the Chinese medicine Tong Xie Yao Fang(TXYF) improves dysfunction in an irritable bowel syndrome(IBS) rat model. METHODS: Thirty baby rats for IBS modeling were separated from mother rats(1 h per day) from days 8 to 21, and the rectum was expanded by angioplasty from days 8 to 12. Ten normal rats were used as normal controls. We examined the effects of TXYF on defection frequency, colonic transit function and smooth muscle contraction, and the expression of 5-hydroxytryptamine(5-HT) and substance P(SP) in colonic and hypothalamus tissues by Western blot and RT-PCT techniques in both normal rats and IBS model rats with characterized visceral hypersensitivity.in normal rats and 4.5 ± 1.58 in IBS model rats(P < 0.001). However, the defecation frequency was significantly decreased(3.0 ± 1.25 vs 4.5 ± 1.58, P < 0.05), while the time(in seconds) of colon transit function was significantly increased(256.88 ± 20.32 vs 93.36 ± 17.28, P < 0.001) in IBS + TXYF group rats than in IBS group rats. Increased colonic smooth muscle tension and contract frequency in IBS model rats were significantly decreased by administration of TXYF. Exogenous agonist stimulants increased spontaneous activity and elicited contractions of colon smooth muscle in IBS model rats, and all of these actions were significantly reduced by TXYF involving 5-HT and SP down-regulation. CONCLUSION: TXYF can modulate the activity of the enteric nervous system and alter 5-HT and SP activities, which may contribute to the symptoms of IBS.

Effect of Tong Xie Yao Fang on endogenous metabolites in urine of irritable bowel syndrome model rats

BACKGROUND Tong Xie Yao Fang is a representative traditional Chinese prescription for the treatment of liver and spleen deficiency,abdominal pain and diarrhea.It has a unique function in the treatment of gastrointestinal dysfunction including irritable bowel syndrome(IBS),is a common functional bowel disease.Its main symptoms are recurrent abdominal pain,diarrhea,constipation or alternations between diarrhea and constipation.There are obvious differences in metabolites between TCM syndromes.By comparing the body fluid metabolism maps of model animals,metabolomics can discover disease biomarkers,analyze the differences in metabolic pathways and understand the pathological process and the metabolic pathways of substances in the body.Thus,the evaluation of animal models tends to be comprehensive and objective.This may provide further understanding between the interaction between Tong Xie Yao Fang and the IBS model.AIM To evaluate the effect of Tong Xie Yao Fang on IBS rats by using metabolomics method.METHODS Wistar rats were used to establish IBS models,and then randomly divided into four groups:A model control group and three Tong Xie Yao Fang treatment groups(high,medium and low doses).A normal,non-IBS group was established.The rats were treated for 2 wk.On days 0 and 14 of the experimental model,urine was collected for 12 h and was analyzed by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry.Nine potential biomarkers were identified,and six major metabolic pathways were found to be related to IBS.RESULTS In the study of metabonomics,nine potential biomarkers including L-serine,4-methylgallic acid,L-threonine,succinylacetone,prolyl-hydroxyproline,valylserine,acetyl citrate,marmesin rutinoside and 5-hydroxy-L-tryptophan were identified in urine,which were assigned to amino acids,organic acids,succinyl and glycosides.Furthermore,the metabolic pathway of L-serine,L-threonine and 5-hydroxy-L-tryptophan was found in the Kyoto Encyclopedia of Genes and Genomes,which mainly involved the metabolism of cysteine and methionine,vitamin B6 metabolism,serotonin synapse,tryptophan metabolism,sphingolipid metabolism,digestion,absorption of protein and amino acid metabolism.These pathways are related to intestinal dysfunction,inflammatory syndrome,nervous system dysfunction and other diseases.CONCLUSION Tong Xie Yao Fang has pharmacological effects on IBS,and its mechanism may be related to the metabolism of the nine potential biomarkers identified above in urine.

Mechanism underlying the protective effect of Kaixin Jieyu Fang on vascular depression following cerebral white matter damage

The Chinese compound Kaixin fieyu Fang can be used to treat vascular depression; however, the underlying mechanism remains unclear. This study established a rat model of chronic cerebral ischemia-caused white matter damage by ligation of the bilateral common carotid arteries. Rats received daily intragastric administration of a suspension of Kaixin ]ieyu Fang powder. After 3, 7 and 21 days of treatment, the degree of white matter damage in the cerebral ischemia rat model was alleviated, Bcl-2 protein and mRNA expression in brain tissue increased, and Bax protein and mRNA expression decreased. These results indicate that Kaixin Jieyu Fang can alleviate cere- bral white matter damage, and the underlying mechanism is associated with regulation of Bcl-2/ Bax protein and mRNA expression, which is one of possible mechanism behind the protective effect of Kaixin Jieyu Fang against vascular depression.