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High-Sensitivity Detection of Fruit Tree Viruses UsingBacterial Magnetic Particles 被引量:9
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作者 Ji-Feng Chen Ying Li +3 位作者 Zhen-Fang Wang Ji-Lun Li Wei Jiang Shao-Hua Li 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2009年第4期409-413,共5页
Prunus necrotic ring spot virus (PNRSV) and grapevine fanleaf virus (GFLV) were detected by fluoroimmunoassay using bacterial magnetic particles (BMPs), and a double antibody sandwich enzyme linked immunosorbent... Prunus necrotic ring spot virus (PNRSV) and grapevine fanleaf virus (GFLV) were detected by fluoroimmunoassay using bacterial magnetic particles (BMPs), and a double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA). For the fluoroimmunoassay, fluorescein isothiocyanate labeled anti-PNRSV antibody or anti-GFLV antibody was conjugated onto BMPs of Magnetospirillum gryphiswaldense MSR-1. With this method, a very low minimum antigen concentration (1×10^6 dilution of the original sample concentration) could be detected. Using DAS-ELISA, the minimum antigen detection concentration was the original sample concentration. Thus, comparing these two methods, a BMP-based method could increase the sensitivity up to six orders of magnitude (10^6) higher than an ELISA-based method of detection PNRSV and GFLV. 展开更多
关键词 bacterial magnetic particle detection sensitivity ELISA FLUOROIMMUNOASSAY grapevine fanleaf virus prunus necrotic ring spot virus.
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