Tanshinone ⅡA improves Alzheimer’s disease via RNA nuclearenriched abundant transcript 1/microRNA-291a-3p/member RAS oncogene family Rab22a axis

BACKGROUND Alzheimer’s disease(AD)is a neurodegenerative condition characterized by oxidative stress and neuroinflammation.Tanshinone ⅡA(Tan-ⅡA),a bioactive compound isolated from Salvia miltiorrhiza plants,has shown potential neuroprotective effects;however,the mechanisms underlying such a function remain unclear.AIM To investigate potential Tan-ⅡA neuroprotective effects in AD and to elucidate their underlying mechanisms.METHODS Hematoxylin and eosin staining was utilized to analyze structural brain tissue morphology.To assess changes in oxidative stress and neuroinflammation,we performed enzyme-linked immunosorbent assay and western blotting.Additionally,the effect of Tan-ⅡA on AD cell models was evaluated in vitro using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.Genetic changes related to the long non-coding RNA(lncRNA)nuclear-enriched abundant transcript 1(NEAT1)/microRNA(miRNA,miR)-291a-3p/member RAS oncogene family Rab22a axis were assessed through reverse transcription quantitative polymerase chain reaction.RESULTS In vivo,Tan-ⅡA treatment improved neuronal morphology and attenuated oxidative stress and neuroinflammation in the brain tissue of AD mice.In vitro experiments showed that Tan-ⅡA dose-dependently ameliorated the amyloid-beta 1-42-induced reduction of neural stem cell viability,apoptosis,oxidative stress,and neuroinflammation.In this process,the lncRNA NEAT1-a potential therapeutic target-is highly expressed in AD mice and downregulated via Tan-ⅡA treatment.Mechanistically,NEAT1 promotes the transcription and translation of Rab22a via miR-291a-3p,which activates nuclear factor kappa-B(NF-κB)signaling,leading to activation of the pro-apoptotic B-cell lymphoma 2-associated X protein and inhibition of the anti-apoptotic B-cell lymphoma 2 protein,which exacerbates AD.Tan-ⅡA intervention effectively blocked this process by inhibiting the NEAT1/miR-291a-3p/Rab22a axis and NF-κB signaling.CONCLUSION This study demonstrates that Tan-ⅡA exerts neuroprotective effects in AD by modulating the NEAT1/miR-291a-3p/Rab22a/NF-κB signaling pathway,serving as a foundation for the development of innovative approaches for AD therapy.

Ecological migration effects on the Tianchi Scenic Area in Xinjiang:from the perspective of migrant farmer families

As an important approach for relieving conflicts between ecosystems and human activities in the Tianchi Scenic Area, Xinjiang of China, an ecological migration policy was implemented, aiming to improve the regional ecological environment. The case of resettlement was closely related to the smooth implementation of the migration project. Based on the questionnaires and farmer family interviews, both changes in the ecological environment and the living conditions of migrant farmer families in the Tianchi Scenic Area were investigated in this study. The objectives were to assess the benefits of the ecological migration policy and to analyze the effects of ecological migration on migrant farmer families. The results showed that （1） awareness of the need for ecological environmental protection and the types of migrant families had a great influence on the willingness to migration; （2） since the implementation of the ecological migration project, the ecological environment in the Tianchi Scenic Area had improved significantly, particutarly in terms of returning farmland to forestry, while pressures on grassland had also been relieved to some extent; （3） the major income sources and occupations of the migrant farmer families changed, the range of their income sources declined, and their per capita incomes decreased at different degrees; and （4） nearly half of the farmer families were not satisfied with the living environment of new settlements and the decline in the quality of life had significant influences on the attitudes of migrants. In order to ensure the successful implementation of an ecological migration project, it is essential to enhance farmer awareness of needs for ecological environmental protection, and to solve resettlement problems properly.

集体经营性建设用地入市农户满意度影响因素研究——基于Logistic-ISM模型的分析

农户满意程度可以反映政策改革成效,探究农户集体经营性建设用地入市满意度影响因素,有助于评价政策实施效果,同时对提升农村发展和农民幸福感也有一定的现实意义.采用问卷调查和访谈的方式,选取重庆市大足区作为研究区域,基于Logistic模型确定农户满意度的影响因素,结合ISM模型分析各影响因素之间的层次关系,对农民的政策实施满意度影响因素进行实证分析.研究结果显示:(1)针对入市实施效果,绝大部分农户持满意态度,不满意的地方集中在收益分配不及时和环境污染方面;(2)家中是否有村干部、生态环境变化情况、是否参与表决、分配公平性、入市实际收益、程序透明性、入市风险存在情况、对入市土地所有者的认知对入市农户满意度有显著影响.其中对入市土地所有者的认知、入市风险存在情况、入市实际收益为表层直接因素,中层间接因素有生态环境变化情况、是否参与表决、分配公平性以及程序透明性,家中是否有村干部为深层根源因素.应进一步完善土地产权制度,明晰入市到期后土地权属问题;健全监督管理机制,建立集体经营性建设用地入市单位的产业目录和准入标准制度;完善收益分配机制,制定集体土地入市指导性分配意见;将村集体负责人纳入政府基层领导队伍体系,提高程序透明性.

A Family of Global Attractors for the Generalized Kirchhoff-Beam Equations

In this paper, we discuss the existence and uniqueness of global solutions, the existence of the family of global attractors and its dimension estimation for generalized Beam-Kirchhoff equation under initial conditions and boundary conditions, using the previous research results for reference. Firstly, the existence of bounded absorption set is proved by using a prior estimation, then the existence and uniqueness of the global solution of the problem is proved by using the classical Galerkin’s method. Finally, Housdorff dimension and fractal dimension of the family of global attractors are estimated by linear variational method and generalized Sobolev-Lieb-Thirring inequality.

Analysis on Irrational Decision Making of Tobacco Family Farmers from the Perspective of Optimal Scale of Efficiency

The larger the difference between the willingness scale of tobacco family farmers and the optimal scale of efficiency,the greater the degree of irrationality,and the higher the decision making risk.With the aid of DEA model,this study calculated the optimal scale of efficiency of Guiyang tobacco family farms.Using the ratio of willingness scale and efficiency optimal scale,it measured the degree of irrationality of family farmers.In addition,with the help of multiple linear regression model,it explained the irrational decision making mechanism of family farmers.Finally,it made a portrait of farmers who tend to make irrational decisions,to find specific farmers and guide them in their production and operation,reduce the risk of planting scale decision making and stabilize the sustainable development of the tobacco industry.

A Story About Mary's Family

The students were having an English class. "Today you can write a story about your family.I want you to tell me something about your parents and your brothers and sisters, "said Mr Shute. Mary raised her hand."What kind of thing shall I write about my parents? "she asked. "Well,"said Mr Shute. "Write about

Similar clinical characteristics of familial and sporadic inflammatory bowel disease in South Korea

AIM:To investigate differences of clinical characteristics and disease courses between familial and sporadic inflammatory bowel disease(IBD)patients.METHODS:We obtained clinical data on Crohn’s disease(CD)(n=691)and ulcerative colitis(n=1113)from a tertiary referral medical center between 2005and 2012.Seventeen patients(2.5%)with CD and27 patients(2.4%)with ulcerative colitis(UC)were identified as having a familial history of IBD,including the first and second degree relatives.For each control case,three times the number of age-,sex-,and diagnosis year-matched CD and UC patients,without a family history of IBD,were randomly selected in this case control study.RESULTS:There were no significant differences in age or main symptom at diagnosis,extraintestinal manifestation,location/extent,behavior of disease activity,number of hospitalizations,number of operations,operation type,number of relapses,or oral medical treatment between familial and sporadic CD and UC patients.Median(min-max)follow-up periods after diagnosis of familial CD and sporadic CD patients were 84(24-312)and 36(8-240)mo,respectively(P=0.008).Familial CD patients more frequently used anti-tumor necrosis factor(TNF)antibodies compared to sporadic CD patients(17.6%vs 0%,P=0.014).CONCLUSION:In conclusion,a family history of IBD does not seem to be an important predictive factor affecting clinical characteristics or disease course even if there is a more frequent use of anti-TNF antibodies in familial CD patients compared to sporadic CD patients.

The Impact of China's Fiscal Expenditure in Agriculture on Farmer's Income

Based on the account of the vital role which the national finance played in supporting agriculture,the thesis presents the current situation of financial support for agriculture in China,in terms of the limited scale and irrational structure of financial support expenditure in agriculture.On the basis of the brief introduction of the current level of Chinese farmers' income,the thesis discusses the effects of scale and irrational structure of financial support expenditure in agriculture on the farmers' income,according to the related data of financial support expenditure in agriculture and rural per capita net income.The results indicate that the calculating regression equation has a remarkable explanation power,reflecting the positive role of financial support expenditure in agriculture played by national finance in increasing the farmers' income.As regards the structure of financial support expenditure in agriculture at present,according to the proportion from high to low,the expenditure primarily consists of three parts from high to low in sequence as follows:productive expenditure and funds for public undertakings,capital construction expenditure,and rural relief expenditure plus science and technology funds.Such sequence deviates from the marginal production effects of financial support expenditure in agriculture and the correlation of farmers' income,which demonstrates the severe irrationality of the structure of financial support expenditure in agriculture.Corresponding countermeasures are put forward as follows:on one hand,we should continue intensifying the efforts to support agriculture financially in order to form a regular increase mechanism;on the other hand,we should adjust and optimize the structure of financial support expenditure in agriculture,and further crystallize the investment flows.

Structure and function of the contactin-associated protein family in myelinated axons and their relationship with nerve diseases

The contactin-associated protein （Caspr） family participates in nerve excitation and conduction, and neurotransmitter release in myelinated axons. We analyzed the structures and functions of the Caspr family- CNTNAP1 （Casprl）, CNTNAP2 （Caspr2）, CNTNAP3 （Caspr3）, CNTNAP4 （Caspr4） and CNTNAP5 （Caspr5）, Casprl-5 is not only involved in the formation of myelinated axons, but also participates in maintaining the stability of adjacent connections. Casprl participates in the formation, differentiation, and proliferation of neurons and astrocytes, and in motor control and cognitive function. We also analyzed the relationship between the Caspr family and neurodegenerative diseases, multiple sclerosis, and autoimmune encephalitis. However, the effects of Caspr on disease course and prognosis remain poorly understood. The effects of Caspr on disease diagnosis and treatment need further investigation.

Adenomas of the common bile duct in familial adenomatous polyposis

Familial adenomatous polyposis(FAP)or Gardner’s syndrome is often accompanied by adenomas of the stomach and duodenum.We experienced a case of adenomas of the common bile duct in a 40-year-old woman with FAP presenting with acute cholangitis.Only 8 cases of adenomas or adenocarcinoma ofthe common bile duct have been reported in the literature in patients with FAP or Gardner’s syndrome.Those patients presented with acute cholangitis or pancreatitis.Local excision or Whipple procedure may be the reasonable surgical option.

Family history and disease outcomes in patients with Crohn's disease:A comparison between China and the United States

AIM To investigate the differences in family history of inflammatory bowel disease(IBD) and clinical outcomes among individuals with Crohn's disease(CD) residing in China and the United States.METHODS We performed a survey-based cross-sectional study of participants with CD recruited from China and the United States.We compared the prevalence of IBD family history and history of ileal involvement,CD-related surgeries and IBD medications in China and the United States,adjusting for potential confounders.RESULTS We recruited 49 participants from China and 145 from the United States.The prevalence of family history of IBD was significantly lower in China compared with the United States(China:4.1%,United States:39.3%).The three most commonly affected types of relatives were cousin,sibling,and parent in the United States compared with child and sibling in China.Ileal involvement(China:63.3%,United States:63.5%) and surgery for CD(China:51.0%,United States:49.7%) were nearly equivalent in the two countries.CONCLUSION The lower prevalence of familial clustering of IBD in China may suggest that the etiology of CD is less attributed to genetic background or a family-shared environment compared with the United States.Despite the potential difference in etiology,surgery and ileal involvement were similar in the two countries.Examining the changes in family history during the continuing rise in IBD may provide further insight into the etiology of CD.

Research on the effects of family care degree on stigma and psychosocial adaptation among the patients with Parkinson’s disease

Objective:This study aimed to investigate the status quo of family care degree,stigma and psychosocial adaptation of the patients with Parkinson’s disease(PD),and discuss the effects of family care degree on stigma and psychosocial adaptation among the patients with PD.Methods:This study was conducted between September 2018 and April 2019 on patients with PD who were admitted to a Ter tiary Grade A hospital in Dalian city.A total of 246 PD patients were investigated by convenience sampling method using self-designed general information questionnaire,along with family care index(APGAR),24-item Stigma Scale for Chronic Illness(SSCI),and Psychosocial Adjustment Scale(PAS)for PD.And patients were divided into two groups according to the level of family care degree and compared with stigma and psychosocial adaptation.Results:The total score of family care for patients with PD was 6.97±2.46,family function was good in 132 cases(53.7%),moderate barrier in 92 cases(37.4%),and severe barrier in 22 cases(8.9%).The scores of stigma and psychosocial adaptability and their dimensions in the group with high degree family care were higher than the low degree group.Conclusions:The overall level of family care for PD patients was poor.Improving family care degree can help patients with PD to reduce stigma and improve psychosocial adaptation.

A Family of Global Attractors for a Class of Generalized Kirchhoff-Beam Equations

The initial boundary value problem for a class of high-order Beam equations with quasilinear and strongly damped terms is studied. Firstly, the existence and uniqueness of the global solution of the equation are proved by prior estimation and Galerkin finite element method. Then the bounded absorption set is obtained by prior estimation, and the family of global attractors for the high-order Kirchhoff-Beam equation is obtained. The Frechet differentiability of the solution semigroup is proved after the linearization of the equation, and the decay of the volume element of the linearization problem is further proved. Finally, the Hausdorff dimension and Fractal dimension of the family of global attractors are proved to be finite.

The role of DJ-1 complexes and catecholamine metabolism： relevance for familial and idiopathic Parkinson＇s disease

Autosomal recessive mutations in the PARK7 gene,which encodes for the protein DJ-1,result in a loss of function and are a cause of familial Parkinson’s disease（PD）,while increased wild-type DJ-1protein levels are associated with some forms of cancer.Several functions of DJ-1 have been described,with the greatest evidence indicating that DJ-1 is a redox-sensitive protein involved in the regulation of oxidative stress and cell survival.

Treatment of Crohn's disease and familial Mediterranean fever by leukopheresis:Single shot for two targets

Coexistence of Crohn's disease(CD) and familial Mediterranean fever(FMF) is a rare condition and knowledge about this clinical situation is limited with a few case reports in the literature. The treatment of both diseases depends on their individual therapies. However, it is very hard to deal with this coexistence when CD is refractory to standard therapies. Ongoing activity of CD triggers the clinical attacks of FMF and the symptoms like abdominal pain interfere with both disease presentations which can cause problems about diagnostic and therapeutic approach. The main therapeutic agent for FMF is colchicine and diarrhea is the most common side effect of this drug. This side effect also causes problems about management of these diseases when both of them are clinically active. Here we report probably the first case in the literature with coexisting CD and FMF who was successfully treated by leukopheresis since he was refractory to conventional therapies for CD.

PARK1 gene mutation of autosomal dominant Parkinson's disease family

Studies have shown that PARK1 gene is associated with the autosomal dominant inheritance of Parkinson＇s disease. PARK1 gene contains two mutation sites, namely Ala30Pro and Ala53Thr, which are located on exons 3 and 4, respectively. However, the genetic loci of the pathogenic genes remain unclear. In this study, blood samples were collected from 11 members of a family with high prevalence of Parkinson＇s disease, including four affected cases, five suspected cases and two non-affected cases. Point mutation screening of common mutation sites on PARK1 gene exon 4 was conducted using PCR, to determine the genetic loci of the causative gene for Parkinson＇s disease. Gene identification and sequencing results showed that a T base deletion mutation was observed in the PARK1 gene exon 4 of all 11 collected samples. It was confirmed that the PARK1 gene exon 4 gene mutation is an important pathogenic mutation for Parkinson＇s disease.

The Function,Future and Support Elements of Farmer Cooperative Economy Organization

The nature of farmer cooperative economy organization( known as FCEO) determines the fact that the economic effects of farmer cooperative economy organization are as important as its social effects. Many experts,however,now would only focus on its economic function, and either neglect or weaken its social influence. Therefore,this paper introduces the theoretical foundation of the farmer cooperative economy organization,and studies the nature of cooperative economics. Based on those typical cases,the future of cooperative organization and four supporting elements were put forward in this paper.

Study on Influence of Farmers' Relational Network on Their Undertaking Process

We elaborate relevant theories of farmers' relational network,including the Differential Model of Association,the Strength of Weak Tie,Strength of Strong Tie and Favor and Face.The farmers' relational network in the Differential Model of Association can be divided into three layers:strong tie,weak tie and irrelative relationship according to Granovetter theory.These three layers have deep influence on opportunity selection during the undertaking,financing and enterprise development.With rational knowledge of these layers,farmers may exploit undertaking resources.On the basis of these,we made detailed analysis on farmers' selection of relations in the opportunity selection,financing and enterprise development stages.

A Study of Farmers' Rationality Based on Maslow's Hierarchy of Needs

In this paper,on the basis of analyzing farmers' dominant position in society and explanation of farmers' rationality,we make use of Maslow's hierarchy theory of needs to divide farmers' needs into three levels in accordance with the different stages: survival needs,interpersonal harmony and social responsibility,which are the needs hierarchies for farmers to satisfy themselves. We combine Maslow's hierarchy theory of needs and farmers' rationality and confirm that farmers do have rationality and it is in a dynamic condition.

割手密GST基因家族的分析及其对阿特拉津的响应

从全基因组的角度分析了割手密谷胱苷肽⁃S⁃转移酶(GST)基因家族成员的特征,共鉴定出129个GST。通过对割手密GST蛋白的系统发育分析,发现这些GST可以划分为4个亚家族,且每个亚家族内的蛋白排列紧凑,说明同亚族内部基因进化关系均比较亲近。此外,对在阿特拉津处理下GST基因家族中10个F亚族基因的表达水平进行检测,结果表明,根中的SpGSTF1、SpGSTF9、SpGSTF14、SpGSTF16、SpGSTF18、SpGSTF31在阿特拉津胁迫下表达显著上调,推测这6个基因可能在响应阿特拉津胁迫的过程中起重要作用。研究结果有助于揭示割手密GST家族的进化和功能演替,并对进一步了解割手密GST对阿特拉津的解毒响应机制提供理论参考,同时也为培养抗除草剂甘蔗品种提供基因资源。