AIM:To investigate whether silencing Fas-associated phosphatase 1(FAP-1)expression enhances the efficiency of chemotherapy for colon carcinoma with oxaliplatin.METHODS:Expression of FAP-1 in mRNA and protein was detec...AIM:To investigate whether silencing Fas-associated phosphatase 1(FAP-1)expression enhances the efficiency of chemotherapy for colon carcinoma with oxaliplatin.METHODS:Expression of FAP-1 in mRNA and protein was detected by reverse transcription polymerase chain reaction(RT-PCR)and flow cytometry.Small interfering RNA(siRNA)was designed according to the FAP-1 mRNA sequence.Cell proliferation was evaluated by methyl thiazolyl tetrazolium(MTT)assay.Anenxin V-and propidine iodine(PI)were assayed by flow cytometry for the detection of apoptosis. RESULTS:The expression of FAP-1 was increased in SW480 cells after chemotherapy with oxaliplatin. Transfection of FAP-1 siRNA into SW480 cells silenced the expression of FAP-1 and consequently abolished the inhibitory function of Fas/FasL-mediated apoptosis pathway,thus increasing the efficacy of chemotherapy for colon carcinoma with oxaliplatin. CONCLUSION:RNA interference combined with conventional chemotherapy is more effective against colon cancer.展开更多
This work examined the determinants of the adoption of improved Irish potato technologies by farmers in three divisions of the Western Region of Cameroon. Data were collected from 170 farmers from 14 villages in our s...This work examined the determinants of the adoption of improved Irish potato technologies by farmers in three divisions of the Western Region of Cameroon. Data were collected from 170 farmers from 14 villages in our study area using a mixed-method approach—structured questionnaires, focus group discussion, key informant interviews, and participatory observations with individual farmers and farmers belonging to cooperative and common initiative groups. The study employed descriptive statistics and regression analysis to assess the adoption status of farmers and its determinants. The logistic regression analysis showed that farmers’ experience in the cultivation of potatoes, the number of follow-ups, and access to extension facilities after training had a significant positive effect on the adoption of these new technologies while membership to an association had a significant negative effect. Additionally, farmers who received improved seeds from NGOs were more likely to adopt a technology than those who did not. Our results suggested that constant follow-up and training of experienced farmers and the provision of improved potato seeds have the potential of maintaining high rates of Irish potato adoption west in Cameroon irrespective of whether they belong to cooperative or not. We recommend that the number of follow-ups, extension training, and market linkages for farmers should be increased. Additionally, more farmers should join farmer groups such as cooperatives or common initiative groups to increase their awareness rate and adopt improved potato innovation through farm extension.展开更多
基金Supported by Research grants from the Science and Technology Foundation of Guangdong Province,No.2006B36002010,2008B030301092,2009B030801005the Foundation of Health Department of Guangdong Province,No.A2005226+2 种基金the foundation of Guangzhou Science and Technology Bureau,No. 2009Y-C011-1the Natural Science Foundation of Guangdong Province,No.7001592the National Natural Science Foundation of China,No.30973505
文摘AIM:To investigate whether silencing Fas-associated phosphatase 1(FAP-1)expression enhances the efficiency of chemotherapy for colon carcinoma with oxaliplatin.METHODS:Expression of FAP-1 in mRNA and protein was detected by reverse transcription polymerase chain reaction(RT-PCR)and flow cytometry.Small interfering RNA(siRNA)was designed according to the FAP-1 mRNA sequence.Cell proliferation was evaluated by methyl thiazolyl tetrazolium(MTT)assay.Anenxin V-and propidine iodine(PI)were assayed by flow cytometry for the detection of apoptosis. RESULTS:The expression of FAP-1 was increased in SW480 cells after chemotherapy with oxaliplatin. Transfection of FAP-1 siRNA into SW480 cells silenced the expression of FAP-1 and consequently abolished the inhibitory function of Fas/FasL-mediated apoptosis pathway,thus increasing the efficacy of chemotherapy for colon carcinoma with oxaliplatin. CONCLUSION:RNA interference combined with conventional chemotherapy is more effective against colon cancer.
文摘This work examined the determinants of the adoption of improved Irish potato technologies by farmers in three divisions of the Western Region of Cameroon. Data were collected from 170 farmers from 14 villages in our study area using a mixed-method approach—structured questionnaires, focus group discussion, key informant interviews, and participatory observations with individual farmers and farmers belonging to cooperative and common initiative groups. The study employed descriptive statistics and regression analysis to assess the adoption status of farmers and its determinants. The logistic regression analysis showed that farmers’ experience in the cultivation of potatoes, the number of follow-ups, and access to extension facilities after training had a significant positive effect on the adoption of these new technologies while membership to an association had a significant negative effect. Additionally, farmers who received improved seeds from NGOs were more likely to adopt a technology than those who did not. Our results suggested that constant follow-up and training of experienced farmers and the provision of improved potato seeds have the potential of maintaining high rates of Irish potato adoption west in Cameroon irrespective of whether they belong to cooperative or not. We recommend that the number of follow-ups, extension training, and market linkages for farmers should be increased. Additionally, more farmers should join farmer groups such as cooperatives or common initiative groups to increase their awareness rate and adopt improved potato innovation through farm extension.
