In recent years, graffiti has ascended to the ranks of modem new art from public action of street and has derived modem graffiti culture. With the development of graffiti art and the improvement of people＇s aesthetic...In recent years, graffiti has ascended to the ranks of modem new art from public action of street and has derived modem graffiti culture. With the development of graffiti art and the improvement of people＇s aesthetic idea, graffiti has penetrated into various aspects of people＇s life and has been closely integrated with people＇s life in many fields. Fashion design is one of the forms which have the closest relation with modem graffiti. Many famous brands have increased their own vitality and fashion sense of costume through graffiti. In modem fashion design, graffiti has special aesthetic value and personality characteristics, bringing about huge commercial opportunities to merchants. Its personality expression in fashion design involves factors in multi-aspects. This paper makes an in-depth analysis of this problem in simple terms, which is of positive significance to the combination of graffiti and fashion design.展开更多
[Objective] This study aimed to increase the expression level of immun- odominant membrane protein gene （Imp） of phytoplasmas in E. coil BL21 （DE3）. [Method] On the basis of orthogonal experiment, effects of diffe...[Objective] This study aimed to increase the expression level of immun- odominant membrane protein gene （Imp） of phytoplasmas in E. coil BL21 （DE3）. [Method] On the basis of orthogonal experiment, effects of different culture conditions on recombinant bacteria E. coil BL21-pET-28a（＋）-Imp were investigated. Based on the obtained optimal culture condition, effects of different induction conditions on the ex- pression level of Imp protein were explored. The expression level of Imp fusion pro- tein was analyzed by using SDS-PAGE and Gene Tools software. [Result] The re- sults showed that the optimal conditions for culture were at 37℃, pH 7.0, with liq- uid volume of 20% and oscillation speed of 200 r/min, for induction were at 37℃ for 6 h, with initial OD600 of about 1.5 and IPTG final concentration of 0.1 mmol/L. [Conclusion] The expression level of Imp achieved 70.98 mg/L under the optimal conditions. Optimized conditions for expression of Imp fusion protein in E. coil were determined.展开更多
文摘In recent years, graffiti has ascended to the ranks of modem new art from public action of street and has derived modem graffiti culture. With the development of graffiti art and the improvement of people＇s aesthetic idea, graffiti has penetrated into various aspects of people＇s life and has been closely integrated with people＇s life in many fields. Fashion design is one of the forms which have the closest relation with modem graffiti. Many famous brands have increased their own vitality and fashion sense of costume through graffiti. In modem fashion design, graffiti has special aesthetic value and personality characteristics, bringing about huge commercial opportunities to merchants. Its personality expression in fashion design involves factors in multi-aspects. This paper makes an in-depth analysis of this problem in simple terms, which is of positive significance to the combination of graffiti and fashion design.
基金Supported by Science and Technology Project of Shenzhen City (JC200903180710A)~~
文摘[Objective] This study aimed to increase the expression level of immun- odominant membrane protein gene （Imp） of phytoplasmas in E. coil BL21 （DE3）. [Method] On the basis of orthogonal experiment, effects of different culture conditions on recombinant bacteria E. coil BL21-pET-28a（＋）-Imp were investigated. Based on the obtained optimal culture condition, effects of different induction conditions on the ex- pression level of Imp protein were explored. The expression level of Imp fusion pro- tein was analyzed by using SDS-PAGE and Gene Tools software. [Result] The re- sults showed that the optimal conditions for culture were at 37℃, pH 7.0, with liq- uid volume of 20% and oscillation speed of 200 r/min, for induction were at 37℃ for 6 h, with initial OD600 of about 1.5 and IPTG final concentration of 0.1 mmol/L. [Conclusion] The expression level of Imp achieved 70.98 mg/L under the optimal conditions. Optimized conditions for expression of Imp fusion protein in E. coil were determined.
