This study investigates the developmental changes of the lipoprotein lipase （LPL） mRNA level in sheep muscle and its effect on intramuscular fat （IMF） accumulation. Male Kazak and Xinjiang Merino sheep at 2-120 da...This study investigates the developmental changes of the lipoprotein lipase （LPL） mRNA level in sheep muscle and its effect on intramuscular fat （IMF） accumulation. Male Kazak and Xinjiang Merino sheep at 2-120 days old were selected. Six animals of each breed were slaughtered at 2, 30, 60, 90, and 120 days （only the Xinjiang Merino sheep at 120-day old were available） to collect samples from longissimus dorsi muscle for the purpose of determining the IMF content and extracting total RNA that was used to investigate the developmental changes of the LPL mRNA expression by real-time PCR. The results showed that in male Kazak sheep, the IMF content increased with the progress of development and there were significant differences （P〈0.05） between the age groups. However, there was no difference （P〉0.05） between age groups in Xinjiang Merino sheep. Furthermore, the IMF content of the male Kazak sheep was significantly higher （P 〈 0.01） than that of the Xinjiang Merino sheep aged from 30 to 90 days. The highest LPL mRNA expression appeared at day 2 and it was significantly higher than that of all other age groups （P 〈 0.01）, while animals at 60-day old had the lowest LPL mRNA expression in the male Kazak sheep. In Xinjiang Merino sheep, the highest one occurred at 30-day old （P〈0.01）, followed by a continuous decrease to the lowest level at 90-day old, and then it started to increase slightly. At 2 to 60-day old, the LPL mRNA expression was negatively correlated to the IMF content （r=-0.625, P 〈 0.05） in male Kazak sheep, but no such relationship was detected in the male Xinjiang Merino sheep.展开更多
This study was conducted to speed up the process of improving the quality of lamb breeds in China,and to solve the problems that restrict the development of lamb breeds in China,such as low quality of lamb breeds,low ...This study was conducted to speed up the process of improving the quality of lamb breeds in China,and to solve the problems that restrict the development of lamb breeds in China,such as low quality of lamb breeds,low meat production rate,and poor meat quality.By studying molecular markers of fat content in sheep muscles,we searched for polymorphic loci related to fat content in sheep muscles on the ANGPTL4 gene.Among the 9 polymorphic loci retrieved,ANGPTL4 was found.Two polymorphic sites,C724 A and A601 G,were significantly associated with intramuscular fat in mutton.In summary,these two molecular markers can be used as a molecular marker reference when breeding high-quality meat or hair and meat using German Merino sheep breeds.展开更多
[Objectives] This study was conducted to speed up the process of improving the quality of lamb breeds in China,and to solve the problems that restrict the development of lamb breeds in China,such as low quality of lam...[Objectives] This study was conducted to speed up the process of improving the quality of lamb breeds in China,and to solve the problems that restrict the development of lamb breeds in China,such as low quality of lamb breeds,low meat production rate,and poor meat quality. [Methods]By studying molecular markers of fat content in sheep muscle,the CAPN gene was used to find polymorphic sites related to fat content in sheep muscle,and the correlation between two polymorphic sites C724 A and A601G on CAPN1 and CAPN2 and fat content in sheep muscle was analyzed. [Results] The two polymorphic sites,C724 A on CAPN1 and A601G on CAPN2 were significantly related to intramuscular fat in mutton. [Conclusions] These two molecular markers can be used as a molecular marker reference when breeding high-quality meat or hair and meat using German Merino sheep breeds.展开更多
文摘This study investigates the developmental changes of the lipoprotein lipase （LPL） mRNA level in sheep muscle and its effect on intramuscular fat （IMF） accumulation. Male Kazak and Xinjiang Merino sheep at 2-120 days old were selected. Six animals of each breed were slaughtered at 2, 30, 60, 90, and 120 days （only the Xinjiang Merino sheep at 120-day old were available） to collect samples from longissimus dorsi muscle for the purpose of determining the IMF content and extracting total RNA that was used to investigate the developmental changes of the LPL mRNA expression by real-time PCR. The results showed that in male Kazak sheep, the IMF content increased with the progress of development and there were significant differences （P〈0.05） between the age groups. However, there was no difference （P〉0.05） between age groups in Xinjiang Merino sheep. Furthermore, the IMF content of the male Kazak sheep was significantly higher （P 〈 0.01） than that of the Xinjiang Merino sheep aged from 30 to 90 days. The highest LPL mRNA expression appeared at day 2 and it was significantly higher than that of all other age groups （P 〈 0.01）, while animals at 60-day old had the lowest LPL mRNA expression in the male Kazak sheep. In Xinjiang Merino sheep, the highest one occurred at 30-day old （P〈0.01）, followed by a continuous decrease to the lowest level at 90-day old, and then it started to increase slightly. At 2 to 60-day old, the LPL mRNA expression was negatively correlated to the IMF content （r=-0.625, P 〈 0.05） in male Kazak sheep, but no such relationship was detected in the male Xinjiang Merino sheep.
文摘This study was conducted to speed up the process of improving the quality of lamb breeds in China,and to solve the problems that restrict the development of lamb breeds in China,such as low quality of lamb breeds,low meat production rate,and poor meat quality.By studying molecular markers of fat content in sheep muscles,we searched for polymorphic loci related to fat content in sheep muscles on the ANGPTL4 gene.Among the 9 polymorphic loci retrieved,ANGPTL4 was found.Two polymorphic sites,C724 A and A601 G,were significantly associated with intramuscular fat in mutton.In summary,these two molecular markers can be used as a molecular marker reference when breeding high-quality meat or hair and meat using German Merino sheep breeds.
基金Supported by Mutton Sheep Research System (CARS-38)。
文摘[Objectives] This study was conducted to speed up the process of improving the quality of lamb breeds in China,and to solve the problems that restrict the development of lamb breeds in China,such as low quality of lamb breeds,low meat production rate,and poor meat quality. [Methods]By studying molecular markers of fat content in sheep muscle,the CAPN gene was used to find polymorphic sites related to fat content in sheep muscle,and the correlation between two polymorphic sites C724 A and A601G on CAPN1 and CAPN2 and fat content in sheep muscle was analyzed. [Results] The two polymorphic sites,C724 A on CAPN1 and A601G on CAPN2 were significantly related to intramuscular fat in mutton. [Conclusions] These two molecular markers can be used as a molecular marker reference when breeding high-quality meat or hair and meat using German Merino sheep breeds.
基金Supported by the Science and Technology Supporting Project (Agriculture) of Jiangsu Province (BE2011452)the Special Fund Project of the National Broiler Industry Technology System (CARS-42-G23)a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)~~
