Milk fatty acids profiles and milk production from dairy cows fed different forage quality diets

Thirty lactating Holstein cows were used to investigate the effects of different forages quality on milk fatty acids(FA) profiles and production. The cows were assigned to 3 dietary treatments(n = 10 per treatment) in a randomized block design with 3 repeated measures. They were fed the experimental diets for 90 d with 3 days of collection of samples for analysis at about 27 d intervals(samples were collected on days 28,29,30, 58, 59, 60.88, 89 and 90). The treatments were(DM basis): 1) mixed forages diet(MF) consisting of 3.7% Chinese wild rye, 26.7% corn silage and 23.4% alfalfa hay; 2) corn stalk diet 1(CS1) where corn stalk was used to formulate a similar chemical nutrient level to MF; 3) corn stalk diet 2(CS2) which used corn stalk to formulate a similar forage level to MF for the diet. Dry matter intake and BW were similar between treatments, but daily milk yield, milk fat and protein yield decreased(P < 0.05)in CS1 and CS2 compared with MF, with CS2 being the lowest(P < 0.05). In total FA of milk, the compositions of C18:1 c9, C18:3 and unsaturated FA increased(P < 0.05) in CS1 and CS2 compared with MF,and C18:0 and trans-C18:1 were trended to increase(P < 0.10), but C4:0-C16:0 were decreased(P < 0.05).Compared with cows fed CS2, cows receiving CS1 increased the compositions of C4:0 to C12:0 and C18:2(P < 0.05). The results suggests feeding corn stalk could produce a greater proportion of unsaturated fatty acid(UFA) in milk fat without resulting in milk fat depression(MFD) in mid lactation cows, but simply increasing the ratio of concentrate in low forages diets is not an effective way to increase milk fat synthesis and milk production.

Serum Biochemical and Meat Fatty Acid Profiles of Different Chicken Genotypes

Serum biochemical and meat fatty acid profile of different chickens were studied. A total of 144 mixed sex matured chickens of Cosmopolitan (C), Improved Horro (H), Cosmopolitan♂*Improved Horro♀(CH), Improved Horro♂*Cosmopolitan♀ (HC), Indigenous (L) and Koekkoek (KK) were used to determine serum biochemical, of which 36 chickens were also used for fatty acid profile study. Completely randomized design in 6 × 2 factorial arrangements was set up. Serum biochemical and fatty acid profiles were determined by Roche/Hitachi cobas c 501 and gas liquid chromatography (GC) procedures, respectively. There were significant differences (P ≤ 0.001) in Total cholesterol (TC), Triglyceride (TG), Low-density lipoprotein (LDL) and High-density lipoprotein (HDL) among genotypes and between sexes. HDL was inversely related with TC, TG and LDL. Male had significantly higher (P ≤ 0.001) TC, TG, LDL but lower HDL than female. SFA, (Myristic, Pentadecanoic and Palmitic acids) had significantly (P < 0.05) varied among genotypes. However, Margaric (P ≤ 0.01) and stearic (P ≤ 0.001) acids were significantly different between sexes. Myristoleic and Palmitoleic acids significantly vary (P ≤ 0.05) among genotypes and between sexes. Oleic (P ≤ 0.01) and Eicosenoic (P ≤ 0.001) significantly influenced by sex. Moreover, Linoleic had significantly (P ≤ 0.01) affected by genotypes. Nevertheless, α-linolenic acid significantly (P ≤ 0.01) varied between sexes. The serum biochemical differed across genotypes and between sexes. The difference in the number of carbons, double bond and position of the double bond could affect fatty acid profile among genotypes and between sexes. Chicken products with higher level TC, TG, LDL and SFA might affect human health problems. It could also be interesting topic for future studies.

Proximate Composition and Fatty Acid Profile of Beef from Arsi, Borana and Harar Cattle Breeds in Oromia National Regional State, Ethiopia

This study was conducted to determine proximate composition and profile of fatty acid of beef from Arsi, Borana and Harar Cattle breeds in Ethiopia. A total of 39 bulls with three age categories were used for the study. The bulls were purchased from mixed crop livestock system (Arsi and Harar cattle breeds) and Ranch (Borana cattle breed). Complete randomized design was used for the study. Longissimus dorsi muscle was used to evaluate proximate composition and profile of fatty acid. The result of the study indicated that mean of percentage of dry matter, ash, crude fat and crude protein were ranging 22.43 - 24.26, 0.32 - 1.28, 4.32 - 7.88, 17.21 - 22.76, respectively. At age younger than 3 years, Harar bulls contain more crude fat compared to Arsi bulls while the vice versa was true for crude protein. The concentration of polyunsaturated fatty acid (PUFA) was higher than saturated fatty acid (SFA) in the three breeds studied across all age categories. However, significantly higher (P < 0.01) PUFA and lowest (P < 0.05) SFA found in Harar breed whereas the opposite hold true for Arsi bulls. Ratio of n-6: n-3 in the muscle of bulls under the study was ranged from 2.10 to 2.57. Concentration of PUFA and MUF in muscle of the three breeds were significantly affected (P < 0.05) by age. From the study it was concluded that Arsi, Boran and Harar bulls under the three age categories contained more than 3% minimum crude fat that is required to insure palatability of the beef. The higher concentration of PUFA over SFA in all breeds across age categories indicated that the meat from these cattle breeds has less risk hazard to human health. However, a strategy needs to be developed to increase the ratio of n-6: n-3 to the nutritional recommendations by the World Health Organization which is 4:1 to 5:1. Moreover, the cause of the difference in proximate composition and profile of fatty acid between breeds under the study at different age categories needs to be investigated.

Determination of the Fatty Acid Profile of Breast Milk from Nursing Mothers in Bungoma County, Kenya

The fatty acid profile in breast milk of nursing mothers who participated in a Cohort for Vitamin A (COVA) study at the fourth and ninth month of lactation was investigated. Breast milk samples were collected by manual expression and stored at - 20°C until analysis. The fat was extracted from the milk and methylated using the American Oil Chemists’ Society (AOCS) Official Methods with modifications. The separation, identification and quantification of the fatty acid methyl esters was performed by gas chromatography coupled with mass spectrometry (GC-MS). Fat contents of human milk increased significantly between the fourth and ninth month of lactation 0.38 and 1.21 mg·mL-1 respectively;P 0.05) than the average percent of the total unsaturated FAs (48.93%). Similarly, at the 9th month the percentage average of the total saturated fatty acids (15.18%) was significantly lower (P < 0.05) than the average percent of the total unsaturated FAs (31.05%). The results obtained in this study demonstrated that the fat content in breast milk significantly increased in the 9th compared to the 4th month of lactation. The fatty profile was also significantly different with the omega 6 being the dominant at the 9th month compared with the omega - 9 being dominant at the 4th month of lactation.

Proximate Composition,Fatty Acid Profile and Mycotoxin Contamination in Several Varieties of Mexican Maize

In Mexico maize (Zea mays L.) is an important cereal due to excellent taste and nutritional value. Nutritional content and fatty acid profile has been reported in white maize, however, there are several genotypes natives (red, yellow, black, blue, pinto), and many improved hybrids maize of which little is known. Fumonisins and aflatoxins are mycotoxins present in 25% of the world’s cereals, mainly in tropical and subtropical regions around the world. This study presents the analysis of proximate composition (ash, protein, lipids), as well as mycotoxins (fumonisins and aflatoxins) and five different fatty acids, two of saturated fatty acids (palmitic and stearic) and three of polyunsaturated fatty acids (oleic, linoleic and linolenic), evaluated by Gas Chromatography and Flame Detector (GC-DF). Thirty varieties of maize (native and hybrid maize) were collected in states of central region. The total fumonisins were determined using the QuickTox TM extraction and quantificated by QuickScan fumonisins;the aflatoxins were analyzed by commercial ELISA kit. The highest protein level was 10.43 g/100g, 5.63 g/100g for fat, 1.62 g/100g for ash in hybrid maize. In native maize, the highest levels of protein and ash were 10.94 g/100g and 1.45 g/100g for pinto maize. The higher value for fat was 5.45 g/100g in yellow maize. The palmitic and stearic acids, in native maize were higher that hybrid maize, for linoleic acid and linoleinic fatty with a significant difference between native corn. Fumonisins and aflatoxins were contaminated in all genotypes of maize, in allow levels. This information obtained may be considered in maize breeding programs, industrialization processes and healthy diets.

High Cholesterol Disorders, Myocardial Infarction and Its Therapeutics

The current review article discusses several conditions linked to high cholesterol, including their causes, diagnoses, and treatments. Cardiovascular disease is one of the leading causes of death worldwide, and hypercholesterolemia is a standalone risk factor for the condition. Numerous cardiovascular illnesses (CVDs) and disorders, including myocardial infarction, hypertension, peripheral vascular diseases, and coronary heart diseases, were highlighted in this article. Low density lipoprotein (LDL) is the key factor in the majority of CVDs. It adheres to fatty deposits and forms plaques inside artery lumen, preventing the heart and other body organs from receiving enough blood and oxygen. Cardiomyopathies and dyslipidemias are the most common causes of morbidity and mortality in men and place a considerable health cost on society. Age, hypertension, obesity-induced blood pressure, and a rise in plasma cholesterol are all factors that raise the risk of coronary heart disease (CHD). Calorie intake must be controlled, meal composition must be changed, a natural cycle must be established, and circadian timeliness must be maintained in order to live a healthy life. To lower the risks of metabolic and cardiovascular diseases, try to go vegetarian and always consume traditional plant-based diets and herbal remedies. Exercise yoga and aerobics every day to stay physically healthy, and have your blood pressure, blood sugar, and cholesterol levels checked on a regular basis. Follow your family doctor’s recommendations for managing high cholesterol risks and diagnosing CVD, obtain a lipid profile, take the recommended medications, maintain composure, and adopt a positive outlook for a long and healthy life.

Dietary inclusion of a partially defatted black soldier fly(Hermetia illucens)larva meal in low fishmeal-based diets for rainbow trout(Oncorhynchus mykiss)

Background:Recent investigations highlighted that Hermetia illucens(BSF)larva meal can be a valuable alternative protein source for aquafeed production.In this study,in substitution of fishmeal,we used increasing inclusion levels of a partially defatted BSF larva meal in low fishmeal-based diets for rainbow trout(Oncorhynchus mykiss Walbaum)and we evaluated the related implications in terms of growth performance,physical characteristics,proximate and fatty acid(FA)compositions of the fillets,gut and liver histology,and diet digestibility.In a 131-day trial,576 fish(100.1±9.29 g)were randomly allotted to 24 tanks(24 fish/tank,4 replicates/treatment).Six experimental diets were produced to have partial replacement of fishmeal(0,10%,20%,30%,40%and 50%)by increasing levels of BSF meal(0%[BSF0],3%[BSF3],6%[BSF6],9%[BSF9],12%[BSF12]and 15%[BSF15]on as fed basis,respectively).Results:No differences were observed among the treatments for all the considered growth performance parameters.The viscero and hepato-somatic indexes showed significant differences among the treatments,with the highest values observed in the BSF15 group.No differences were recorded in terms of fillet’s physical characteristics,dry matter(DM),crude protein(CP)and ether extract(EE)contents.Total saturated and monounsaturated FA increased,while polyunsaturated FA(particularly n-3 FA)decreased while increasing the HI meal inclusion in the diet.Histopathology of liver and gut was not affected,whereas,in posterior gut,villi were higher in BSF6 and BSF9 compared to BSF3 fish.The apparent digestibility of DM,CP,EE and gross energy did not vary among the treatments.Conclusions:These results suggest that a partially defatted BSF meal could be included up to 15%in low fishmeal-based diets for rainbow trout with no adverse effects on growth performance,fillet’s physical characteristics,gut and liver health,and diet digestibility.On the contrary,the fillet FA composition worsened while increasing the level of BSF meal in the diet.

Discrimination of Chuanminshen violaceum Sheh et Shen from different regions based on fatty acid profiles of roots and leaves

Objectives:The purpose of this paper was to construct a reliable methodology to discriminate the geographical origins of Chuanminshen violaceum Sheh et Shan planted in different regions in Sichuan,China.Materials and methods:Fatty acid profiles of roots and leaves of C.violaceum planted in various regions of Sichuan Province in China,namely Guangyuan(GY),Langzhong(LZ),Jintang(JT),Bazhong(BZ),and Shuangling(SL),were determined using GC-MS followed by multivariate statistical analyses,including orthogonal partial least-squares discriminant analysis and hierarchical clustering analysis.Results:Leaves of C.violaceum showed the highest contents of hexadecatrienoic acid(3.21 g/kg),linoleic acid(6.62 g/kg),andα-linolenic acid(7.24 g/kg),which were all higher than those contained in roots.Chuanminshen violaceum samples collected from LZ,JT,and GY could be clearly distinguished based on fatty acid profiles of leaves and those collected from LZ,GY,and BZ could be clearly distinguished based on fatty acid profiles of roots.Conclusions:Chemometric method is used as a potential approach for analyses of fatty acid profiles of roots and leaves to control the quality of C.violaceum and their powered products.

Effects of total replacement of corn silage with sorghum silage on milk yield,composition, and quality

Background： In the last years, difficulties occurring in corn cultivation（i.e., groundwater shortages, mycotoxin contamination） have been forcing dairy farmers to consider alternative silages. Some experiments conducted on lactating cows have proven that the total replacement of corn silage with sorghum silage did not reduce milk yield.However, this kind of substitution involves supplementing sorghum-based diets with grains, to compensate for the lower starch content of sorghum silage compared to corn silage. Change of silage type and inclusion of starch sources in the diet would influence rumen fermentations, with possible effects on milk composition（i.e., fatty acid profile） and coagulation properties. A worsening of milk coagulation properties would have a negative economic impact in Italy, where most of the milk produced is processed into cheese.This study was designed to compare milk composition and quality, with emphasis on fatty acid profile and coagulation properties, in dairy cows fed two diets based on corn or sorghum silage.Results： The sorghum diet reduced milk yield（P = 0.043） but not 4% fat corrected milk（P = 0.85）. Feeding sorghum silage did not influence milk contents of protein（P = 0.07） and lactose（P = 0.65）, and increased fat content（P = 0.024）.No differences emerged for milk concentrations of saturated（P = 0.61） and monounsaturated fatty acids（P = 0.50）,whereas polyunsaturated fatty acids were lower（P 〈 0.001） for the sorghum diet. Concentrations of n-6（P 〈 0.001） and n-3 fatty acids（P = 0.017） were lower in milk of cows fed the sorghum diet. Milk coagulation properties did not differ between the two diets, except the ＂a30＂（the curd firmness, expressed in mm, 30 min after rennet addition）, that was lower（P = 0.042） for the sorghum diet.Conclusions： Feeding a forage sorghum silage, properly supplemented with corn meal, as total replacement of corn silage maintained milk composition and did not influence negatively milk coagulation properties, which have a great economic relevance for the Italian dairy industry. Thus, silages obtained from forage sorghums could have a potential as substitute of corn silages in dairy cow diets.

Effect of Enteral Nutrition Formula on Fat Absorption and Serum Free Fatty Acid Profiles in Rat with Short-Bowel Syndrome

The effects of enteral nutrition containing long chain triglycerides （LCT） and medium chain triglycerides （MCT） or L-arginine （Arg） on fat absorbability, serum free fatty acid profiles and intestinal morphology in rats with short-bowel syndrome （SBS） were studied using gas chromatography. Twenty-eight Sprague-Dawley rats were randomly assigned to 4 groups： sham operation fed with LCT as control; 85% small bowel resection fed with LCT, MCT/LCT, and Arg/LCT, respectively. SBS rats showed a decrease of fat absorptivity. Enteral nutrition supplemented with MCT could increase fat absorptivity. L-Arginine enhanced enteral nutrition was associated with the elevation of fat absorptivity, possibly due to its enterotrophic effect on remnant small bowel mucosa. LCT group showed a significant deficiency of total free fatty acid and the decreased essential fatty acid content, which was improved in other two SBS groups.

Dietary isoleucine affects muscle fatty acid and amino acid profiles through regulating lipid metabolism and autophagy in hybrid catfish(Pelteobagrus vachelli ♀×Leiocassis longirostris ♂)

The present study explored the impacts of Ile on muscle fatty acid and amino acid profiles,lipid metabolism,and autophagy in hybrid catfish.Seven isonitrogenous(387.8 g/kg protein)semi-purified diets were formulated to contain 5.0(control),7.5,10.0,12.5,15.0,17.5,and 20.0 g Ile/kg diet respectively.The fish(initial weight of 33.11±0.09 g)were randomly assigned to 7 groups for a 56-day trial.Each group has 3 replicates with 30 fish per replicate,fed at 08:00 and 18:00 each day.Results showed that muscle protein and lipid,C14:0,C18:0,C22:0,C14:1,C18:1n-9,polyunsaturated fatty acid(PUFA),Arg,Ile,Ala,Cys,Gly,Tyr,essential amino acid(EAA),and total amino acid(TAA)contents and flavor amino acid(FAA)/TAA in muscle had positive linear and/or quadratic responses to dietary Ile levels(P<0.05).Fatty acid synthase(FAS),stearoyl-Co A desaturase(SCD),acetyl-Co A carboxylase(ACC),and lipoprotein lipase(LPL)activities had positive linear and/or quadratic responses,but carnitine palmitoyl transferase 1(CPT1)activity had a negative response with increasing dietary Ile levels(P<0.05).The m RNA expressions of FAS,SCD,ACC,LPL,fatty acid binding protein 4(FABP4),FATP1,sterol response element-binding protein 1c(SREBP-1c),sequestosome 1(SQSTM1),and adenosine 5’-monophosphateactivated protein kinase(AMPK)had positive linear and/or quadratic responses to dietary Ile levels(P<0.05).The m RNA expressions of hormone-sensitive lipase(HSL),CPT1,peroxisome proliferatoractivated receptor a(PPARa),PPARγ,uncoordinated 51-like kinase 1(ULK1),beclin1(Becn1),autophagy-related protein 9a(Atg9a),Atg4b,Atg7,autophagy marker light chain 3 B(LC3B),and SQSTM1in muscle had negative linear and/or quadratic responses to dietary Ile levels(P<0.05).The p-AMPK and ULK1 protein levels,and p-AMPK/AMPK were decreased by 12.5 g Ile/kg in the diet(P<0.05).Finally,SQSTM1 protein level had the opposite effect(P<0.05).The above results indicate that dietary Ile improves fish muscle fatty acid and amino acid profiles potentially via respectively regulating lipid metabolism and autophagy.The Ile requirement of hybrid catfish(33 to 72 g)were estimated to be 12.63,13.77,13.75,11.45,10.50,12.53 and 12.21 g/kg diet based on the regression analysis of protein,lipid,SFA,PUFA,FAA,EAA,and TAA muscle contents,respectively.

Functional lipidomics in patients on home parenteral nutrition: Effect of lipid emulsions

To investigate the fatty acid-based functional lipidomics of patients on long-term home parenteral nutrition receiving different intravenous lipid emulsions. METHODSA cross-sectional comparative study was carried out on 3 groups of adults on home parenteral nutrition (HPN), receiving an HPN admixture containing an olive-soybean oil-based intravenous lipid emulsion (IVLE) (OO-IVLE; n = 15), a soybean- medium-chain triacylglycerol-olive-fish oil-based IVLE (SMOF-IVLE; n = 8) or HPN without IVLE (No-IVLE; n = 8) and 42 healthy controls (HCs). The inclusion criteria were: duration of HPN ≥ 3 mo, current HPN admixtures ≥ 2 mo and HPN infusions ≥ 2/wk. Blood samples were drawn 4-6 h after the discontinuation of the overnight HPN infusion. The functional lipidomics panel included: the red blood cell (RBC) fatty acid (FA) profile, molecular biomarkers [membrane fluidity: saturated/monounsaturated FA ratio = saturated fatty acid (SFA)/monounsaturated fatty acid (MUFA) index; inflammatory risk: n-6/n-3 polyunsaturated fatty acid (PUFA) ratio = n-6/n-3 index; cardiovascular risk: sum of n-3 eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) = n-3 index; free radical stress: sum of FA trans isomers = %trans index] and FA pathway enzyme activity estimate (delta-9-desaturase = D9D; delta-6-desaturase = D6D; delta-5-desaturase = D5D; elongase = ELO). Statistics were carried out using nonparametric tests. The amount of each FA was calculated as a percentage of the total FA content (relative%). RESULTSIn the OO-IVLE group, the percentage of oleic acid in the RBCs was positively correlated with the weekly load of OO-IVLE (r = 0.540, P = 0.043). In the SMOF-IVLE cohort, the RBC membrane EPA and DHA were positively correlated with the daily amount of SMOF-IVLE (r = 0.751, P = 0.044) and the number of HPN infusions per week (r = 0.753; P = 0.046), respectively. The SMOF-IVLE group showed the highest EPA and DHA and the lowest arachidonic acid percentages (P < 0.001). The RBC membrane linoleic acid content was lower, and oleic and vaccenic acids were higher in all the HPN groups in comparison to the HCs. Vaccenic acid was positively correlated with the weekly HPN load of glucose in both the OO-IVLE (r = 0.716; P = 0.007) and the SMOF-IVLE (r = 0.732; P = 0.053) groups. The estimated activity of D9D was higher in all the HPN groups than in the HCs (P < 0.001). The estimated activity of D5D was lower in the SMOF-IVLE group than in the HCs (P = 0.013). The SFA/MUFA ratio was lower in all the HPN groups than in the HCs (P < 0.001). The n-6/n-3 index was lower and the n-3 index was higher in the SMOF-IVLE group in comparison to the HCs and to the other HPN groups (P < 0.001). The %trans index did not differ among the four groups. CONCLUSIONThe FA profile of IVLEs significantly influenced the cell membrane functional lipidomics. The amount of glucose in the HPN may play a relevant role, mediated by the insulin regulation of the FA pathway enzyme activities.

Sainfoin(Onobrychis viciifolia)silage in dairy cow rations reduces ruminal biohydrogenation and increases transfer efficiencies of unsaturated fatty acids from feed to milk

The effects of replacing grass silage by sainfoin silage in a total mixed ration(TMR)based diet on fatty acid(FA)reticular inflow and milk FA profile of dairy cows was investigated.The experiment followed a crossover design with 2 dietary treatments.The control diet consisted of grass silage,corn silage,concentrate and linseed.In the sainfoin diet,half of the grass silage was replaced by a sainfoin silage.Six rumen cannulated lactating multiparous dairy cows with a metabolic body weight of 132.5±3.6 kg BW^0.75,214±72 d in milk and an average milk production of 23.1±2.8 kg/d were used in the experiment.Cows were paired based on parity and milk production.Within pairs,cows were randomly assigned to either the control diet or the sainfoin diet for 2 experimental periods(29 d per period).In each period,the first 21 d,cows were housed individually in tie-stalls for adaptation,then next 4 d cows were housed individually in climate-controlled respiration chambers to measure CH4.During the last 4 d,cows were housed individually in tie stalls to measure milk FA profile and determine FA reticular inflow using the reticular sampling technique with Cr-ethylenediaminetetraacetic acid disodium salt dihydrate(EDTA)and Yb-acetate used as digesta flow markers.Although the dietary C18:3n-3 intake was lower(P=0.025)in the sainfoin diet group,the mono-unsaturated FA reticular inflow was greater(P=0.042)in cows fed the sainfoin diet.The reticular inflow of trans-9,trans-12-C18:2 and cis-12,trans-10 C18:2 was greater(P≤0.024)in the sainfoin diet group.The cows fed sainfoin diet had a lower(P≤0.038)apparent ruminal biohydrogenation of cis-9-C18:1 and C18:3n-3,compared to the cows fed the control diet.The sainfoin diet group had greater(P≤0.018)C18:3n-3 and cis-9,cis-12-C18:2 proportions in the milk FA profile compared to the control diet group.Transfer efficiencies from feed to milk of C18:2,C18:3n-3 and unsaturated FA were greater(P≤0.0179)for the sainfoin diet.Based on the results,it could be concluded that replacing grass silage by sainfoin silage in dairy cow rations reduces ruminal C18:3n-3 biohydrogenation and improves milk FA profile.

Adulteration detection of edible oils with castor oil by gas chromatography

Developing a method of adulteration detection is critical for protecting customers＂ rights which is a particular concern in food quality. In this study, fatty acid profiles of castor oils were estab-lished by GC and employed to classify 4 types of edible oils and castor oil with multivariate statistical methods. The results indicated that fatty acid profiles of edible oils could be used to classify the 5 kinds of oils. Meanwhile, simulated data test indicated that fatty acid profiles could be used to detect adultera-ted by 5% . Finally, a RF model was built to detect adulteration of edible oils with castor oils by fatty acid composition. The results from cross validation indicated that the oils adulterated by castor oil at low levels （5% 7V/V） could be completely separated from 4 kinds of edible oils. Therefore this model could be used to detect adulteration of 4 kinds of edible oil with castor oils.

A Combination of Wheat Bran and Vegetable Oils as Feedstuff in Laying Hens’Diet:Impact on Egg Quality Parameters

Wheat bran (WB) is an important side product of the milling industry and plays an increasing role in animal feeding. Despite its nutritional value, its application is limited as a result of its pronounced fiber content. Taking into account the potential use of WB supplementation in feed for laying hens thereby replacing human edible foodstuffs, the influence of different WB levels (0 g·kg-1, 75 g·kg-1, and 150 g·kg-1) combined with sunflower or rapeseed oil on egg quality was studied. Among the egg-related quality parameters investigated, eggshell cleanliness, shell rigidity, egg weight, haugh units, yolk and albumen mass, color, pH value, dry matter, crude protein, crude ash, fatty acid spectrum, cholesterol and α-tocopherol of the yolk were monitored. No negative effect of WB supplementation was observed in terms of egg weight, shell weight, albumen weight, yolk weight, haugh unit, shell thickness and eggshell breaking strength. However, certain parameters (i.e. α-tocopherol content, Σn-3 PUFA) were enhanced by feeding 75 g·kg-1 and 150 g·kg-1 WB. The use of WB (75 g·kg-1 and 150 g·kg-1) had no negative effect on egg quality and can, therefore, be recommended for laying hen diets up to 150 g·kg-1. Additionally, the application of rapeseed oil high in Σn-3 PUFA resulted in yolks exhibiting a low Σn-6 PUFA/Σn-3 PUFA ratio.

Practical Application of Diets Rich in Ground Linseed to Enhance Omega-3 Milk Production in Holstein Dairy Farms Located in Northeast Iran

In an attempt to enhance organic omega-3 milk production, a practical study was performing in 7 Holstein dairy farms in Northeast Iran. The experiment was conducted at the ＂ShafashirToos industrial dairy farmers production and distribution cooperative＂ located in Northeast Iran, between June 2010 and December 2010. In Golshid-Mashhad Co., cows were fed with no linseed containing diets （NO-Lin）, while in the other farms （Hanaei Co., Ghasemi Co., Taheri Co., Teliseh Co., Arasp Co. and Navingholshid-Khorasan Co.）, cows were fed diets containing 9% ground linseed （Lin）. Cows were used in a randomized complete block experimental design. Diets were provided as TMR. Concentration of fat, protein and lactose in milk was measured. Milk fat extraction and fatty acid separation were determined. There were no differences in the most of the milk fatty acid profiles of cows in farms that received ground linseed but feeding Lin diets compared with NO-Lin, significantly decreased （P 〈 0.05） C8：0-C16：0 concentration and it increased those of C 18：1 n9trans, C 18：1 n9cis, C 18：2 trans and C 18：3 n3. As a result, farms with Lin diets had better milk fatty acid profiles with doubled in a-Linolenic acid concentration and increased in mono and polyunsaturated fatty acids and decrease in medium chain and saturated fatty acids compared with those of the NO-Lin diet. These results clearly demonstrate the possibility for sustainable production of organic omega-3 milk with levels ofC18：3 FA up to 2.4 times higher than typical levels in dairy fat. The manufacture of omega-3 enriched milk and milk products could supply dietary fatty acids at levels that may henefit health, without the need for unrealistic changes to eating habits.

Potential of <i>Dictyosphaerium</i>sp. LC172264 Concomitant Remediation of Cassava Wastewater and Accumulation of Lipids for Biodiesel Production

As a way of making algal feedstock feasible for biofuel production, simultaneous utilization of microalga Dictyosphaerium sp. LC172264 for cassava wastewater remediation and accumulation of lipids for biodiesel production was investigated. The algal biomass, lipid contents and composition were measured from the autotrophic, heterotrophic and mixotrophic cultured algal cells. Physicochemical parameters of the cassava wastewater and bioremediation potentials were measured. Biodiesel properties were deduced and compared with the standards. The results showed that mixotrophic culture was the best for both biomass accumulation (1.022 g/L) and lipid contents (24.53%). Irrespective of the culture condition, the predominant fatty acids were similar and included 11-Octadecenoic acid (vaccenic acid (C19H36O2), oleic acid (C18H34O2) and 14-methyl pentadecanoic acid (isopalmitic acid (C17H34O2). The percentage reduction of total dissolved solids was 79.32% and 89.78% for heterotrophy and mixotrophy respectively. Biochemical oxygen demand was 72.95% and 89.35%, chemical oxygen demand was 72.19% and 84.03% whereas cyanide contents reduced from the initial value of 450 mg/L to 93.105 (79.31%) and 85.365 mg/L (81.03%) respectively. Dictyosphaerium sp. showed good growth and lipid production under mixotrophic condition and produced good quality biodiesel under the three cultivation modes. Even though both mixotrophic and heterotrophic conditions had good promise of cassava wastewater remediation by Dictyosphaerium sp., mixotrophy showed superiority.

The Biotechnological and Seafood Potential of Stichopus regalis

Sea cucumbers are highly used not only for the production of the bêche-de-mer, but also recent studies have been showing the high potential of these marine invertebrates on the pharmaceutical industry, as they are rich in bioactive compounds with important functions. Stichopus regalis extracts (1 mg·mL-1) were used in antioxidant, antimicrobial and antitumor assays. The lipid profile was also evaluated. No significant antioxidant activity was detected in both methanolic and dichloromethane extracts. The methanolic fraction showed the highest antimicrobial potential against Candida albicans with an IC50 of 475.4 μg·mL-1. In the antitumor assays, the dichloromethane fraction showed a high potential for both cell lines, as revealed by the MTT method. The total fat content was 3.63% ± 0.11% and the fatty acid profile revealed the highest amount in C16:0 (9.43% ± 0.77%), C18:0 (12.43% ± 0.83%), C18:1 ω7 (5.63% ± 0.33%), EPA (12.49% ± 0.15%), DHA (7.35% ± 0.02%), ARA (19.29% ± 0.14%) and a ω3/ω6 ratio of 1.078. These findings led us to suggest the potential use of S. regalis as a new source of bioactive compounds with pharmacological potential and its nutritional benefits for human health.

Effects of essential oils on performance,egg quality, nutrient digestibility and yolk fatty acid profile in laying hens

The study was conducted to investigate the effect of essential oils on performance, egg quality, nutrient digestibility and yolk fatty acid profile in laying hens. A total of 960 Lohmann laying hens aged 53 weeks were enrolled, under 4 different treatment diets supplemented with 0, 50.100 and 150 mg/kg essential oils(Enviva EO, Dupont Nutrition Biosciences ApS, Denmark), respectively. Each treatment was replicated8 times with 30 birds each. Birds were fed dietary treatment diets for 12 weeks(54 to 65 weeks). For data recording and analysis, a 12-week period was divided into 3 periods of 4 weeks' duration each: period 1(54 to 57 weeks), period 2(58 to 61 weeks), and period 3(62 to 65 weeks). For the diet supplemented with Enviva EO, hen-day egg production and the feed conversion ratio(FCR) were significantly improved(P < 0.05) at weeks 58 to 61, and the eggshell thickness was significantly increased(P < 0.05) at week 65.However, egg production, egg weight, feed intake, FCR and other egg quality parameters(albumen height, Haugh unit, egg yolk color and eggshell strength) were not affected by the dietary treatment. In addition, compared with the control diet, protein digestibility in the 100 mg/kg Enviva EO treatment group was significantly increased(P < 0.05), and fat digestibility in the 100 and 150 mg/kg Enviva EO treatment groups was significantly decreased(P < 0.05), but Enviva EO had no effect on energy apparent digestibility. Saturated fatty acid(SFA) and monounsaturated fatty acid(MUFA) gradually decreased and polyunsaturated fatty acid(PUFA) increased with Enviva EO supplementation, but the difference was not significant. The data suggested that the supplementation of essential oils(Enviva EO) in laying hen diet did not show a significant positive effect on performance and yolk fatty acid composition but it tended to increase eggshell thickness and protein digestibility, especially at the dose of 50 mg/kg.

Determination of fruit characteristics,fatty acid profile and total antioxidant capacity of Mespilus germanica L.fruit

Objective:To determine fruit characteristics,fatty acid profile and total antioxidant capacitiy of first cultured Mespilus germanica L.Methods:A total of 15 fruits were taken randomly from four directions of adult trees.Then the physical and chemical properties of first cultured medlar fruit(Istanbul/Turkey)were measured by using refractometer,colorimeter,spectrophotometer and gas chromatograph mass spectrometer,respectivly.Results:In the fruit studied,the results showed that palmitic acid,stearic acid,oleic acid,linoleic acid,linolenic acid,arachidic acid and behenic acid were the most abundant fatty acids(FAs),and the main FA was palmitic acid[(35.35±1.20)%].The percentage of linoleic acid and stearic acid in this fruit oil were(29.10±1.70)%and(8.53±0.25)%,respectively.As a result of the analysis,the total antioxidant capacity of medlar fruit was(1.1±0.2)mmol trolox equivalents/L.Conclusions:The present study has demonstrated the concentrations of FAs and the antioxidantive capacity of first cultured Istanbul medlar fruits by using many tested methods.It is proved that in our daily life,medlar fruit plays a significant role with its nutrition and health effect.