BACKGROUND： Liver steatosis affects 20%-30% of adults.Because of the increasing gap between graft supplies and demands, livers with steatosis are frequently used in liver transplantation. But severely steatotic liver...BACKGROUND： Liver steatosis affects 20%-30% of adults.Because of the increasing gap between graft supplies and demands, livers with steatosis are frequently used in liver transplantation. But severely steatotic liver grafts are associated with a high risk of intraoperative and postoperative complications. Accurate assessment of fat content of donor livers and monitoring of the extent of steatosis in recipients are required for liver transplantation. The present study aimed to determine the correlation between liver echogenicity and fat content, and to evaluate the use of an ultrasonic integrated backscatter system（IBS） in the assessment of changes in fat content after liver transplantation.METHODS： Seventy-nine consecutive patients receiving liver grafts from living donors were evaluated in our center. Of these recipients, 67 survived for more than two years and were included in this study. Each liver graft was evaluated with IBS and ultrasound before operation and the fat content was estimated. The fat content of the grafts in the recipients was again assessed with ultrasound at 18 months after surgery.RESULTS： A correlation was detected between each graft＇s IBS value and its fat content（P=0.001）. The IBS value in fatty grafts with various degrees of steatosis was significantly decreased in 3（P=0.02）, 12, 15 and 18（P=0.001） months after orthotopic liver transplantation. The IBS value returned to normal in all patients in 18 months after liver transplantation.CONCLUSIONS： Decreased fat content in steatotic grafts can be observed in all recipients. Ultrasonic IBS is useful in determining the steatotic degree of grafts in donors as well as in monitoring the grafts after liver transplantation.展开更多
Prunus mahaleb L.seeds are widely used in the baking industry as spice for flavouring.While achieving the mahaleb powder for commercial use in local companies,the seeds are sometimes mixed unintentionally with their k...Prunus mahaleb L.seeds are widely used in the baking industry as spice for flavouring.While achieving the mahaleb powder for commercial use in local companies,the seeds are sometimes mixed unintentionally with their kernels(stony shell)during sieving procedure.As the chemical and biological properties of the kernel of seeds are not well known,the kernel,seeds and the market sample obtained from a local supplier are decided to investigate individually.The antioxidant capacity and the trace element analyses of the seeds and kernels were performed by TEAC and CUPRAC assays and ICP-MS.The market sample was analysed for nutrient and oil composition.Fatty acid composition of the oil was determined with GC-FID and GC-MS methods.The chemical composition of the methanol extract of the seeds and kernels was studied by LC-MS/MS.Trace elements Mn,Co,Ni,Cu,Zn and Se were determined in the methanol extracts,ethyl acetate and water fractions.Mushroom tyrosinase enzyme inhibitions of 33 different extracts and fractions of various waste parts of the plant(kernel,fruit,twigs,flos,leaves)were investigated.The nutritional values showed that the market sample had high nutritious properties.The fatty acid content of the oil was found to be valuable in remedying dysfunctions of the body.Kernel ethylacetate fraction showed the highest tyrosinase enzyme inhibition.The role of copper element on enzyme activity was determined.The kernels are accepted as a waste material may also be evaluated as a good promising choice for food,cosmetic and drug industry.展开更多
文摘BACKGROUND： Liver steatosis affects 20%-30% of adults.Because of the increasing gap between graft supplies and demands, livers with steatosis are frequently used in liver transplantation. But severely steatotic liver grafts are associated with a high risk of intraoperative and postoperative complications. Accurate assessment of fat content of donor livers and monitoring of the extent of steatosis in recipients are required for liver transplantation. The present study aimed to determine the correlation between liver echogenicity and fat content, and to evaluate the use of an ultrasonic integrated backscatter system（IBS） in the assessment of changes in fat content after liver transplantation.METHODS： Seventy-nine consecutive patients receiving liver grafts from living donors were evaluated in our center. Of these recipients, 67 survived for more than two years and were included in this study. Each liver graft was evaluated with IBS and ultrasound before operation and the fat content was estimated. The fat content of the grafts in the recipients was again assessed with ultrasound at 18 months after surgery.RESULTS： A correlation was detected between each graft＇s IBS value and its fat content（P=0.001）. The IBS value in fatty grafts with various degrees of steatosis was significantly decreased in 3（P=0.02）, 12, 15 and 18（P=0.001） months after orthotopic liver transplantation. The IBS value returned to normal in all patients in 18 months after liver transplantation.CONCLUSIONS： Decreased fat content in steatotic grafts can be observed in all recipients. Ultrasonic IBS is useful in determining the steatotic degree of grafts in donors as well as in monitoring the grafts after liver transplantation.
文摘Prunus mahaleb L.seeds are widely used in the baking industry as spice for flavouring.While achieving the mahaleb powder for commercial use in local companies,the seeds are sometimes mixed unintentionally with their kernels(stony shell)during sieving procedure.As the chemical and biological properties of the kernel of seeds are not well known,the kernel,seeds and the market sample obtained from a local supplier are decided to investigate individually.The antioxidant capacity and the trace element analyses of the seeds and kernels were performed by TEAC and CUPRAC assays and ICP-MS.The market sample was analysed for nutrient and oil composition.Fatty acid composition of the oil was determined with GC-FID and GC-MS methods.The chemical composition of the methanol extract of the seeds and kernels was studied by LC-MS/MS.Trace elements Mn,Co,Ni,Cu,Zn and Se were determined in the methanol extracts,ethyl acetate and water fractions.Mushroom tyrosinase enzyme inhibitions of 33 different extracts and fractions of various waste parts of the plant(kernel,fruit,twigs,flos,leaves)were investigated.The nutritional values showed that the market sample had high nutritious properties.The fatty acid content of the oil was found to be valuable in remedying dysfunctions of the body.Kernel ethylacetate fraction showed the highest tyrosinase enzyme inhibition.The role of copper element on enzyme activity was determined.The kernels are accepted as a waste material may also be evaluated as a good promising choice for food,cosmetic and drug industry.
