Identification of SSR markers linked to the abscission of cotton boll traits and mining germplasm in Cotton

Background Cotton is an economically important crop.It is crucial to find an effective method to improve cotton yield,and one approach is to decrease the abscission of cotton bolls and buds.However,the lack of knowledge of the genetic and molecular mechanisms underlying cotton boll abscission traits has hindered genetic improvements.Results Pearson’s correlation analysis revealed a significant positive correlation between boll abscission rates 1(AR1)and boll abscission rates 2(AR2).A genome-wide association study was conducted on 145 loci that exhibited high polymorphism and were uniformly distributed across 26 chromosomes(pair).The study revealed 18,46,and 62 markers that were significantly associated with boll abscission,fiber quality,and yield traits(P<0.05),explaining 1.75%–7.13%,1.16%–9.58%,and 1.40%–5.44%of the phenotypic variation,respectively.Notably,the marker MON_SHIN-1584b was associated with the cotton boll abscission trait,whereas MON_CGR5732a was associated with cotton boll abscission and fiber quality traits.Thirteen of the marker loci identified in this study had been previously reported.Based on phenotypic effects,six typical cultivars with elite alleles related to cotton boll abscission,fiber quality,and yield traits were identified.These cultivars hold great promise for widespread utilization in breeding programs.Conclusions These results lay the foundation for understanding the molecular regulatory mechanism of cotton boll abscission and provide data for the future improvement of cotton breeding.

Study on crustal thickness and the prediction of prolific depressions:the Bohai Basin as an example

The deep crustal structure is closely related to oil and gas reserves.Predicting the oil and gas enrichment of depressions based on the Moho depth and crustal thickness is a promising research topic with significant implications for guiding exploration in petroliferous basins.In this study,seismic data were used as a constraint on the use of satellite gravity anomaly inversion to obtain the distribution of Moho depth and crustal thickness in the Bohai Basin.Stretching factors were calculated to analyze the differential distribution of deep crustal structural activity.Four indicators,including the minimum Moho depth,minimum crustal thickness,sum of Moho stretching factors,and sum of crustal stretching factors,were selected.Principal component analysis was applied to reduce the dimensionality of the multi-indicator system and obtain an oil and gas enrichment score for quantitative prediction of favorable prolific depressions.The deviation between the inverted Moho depth and seismic constraints was small;thus,the data effectively reflect the variations in the characteristics of each depression.The analysis revealed significant statistical features related to the minimum Moho depth/crustal thickness and the sum of Moho/crustal stretching factors associated with prolific depressions.Based on the oil and gas enrichment score,the depressions were classified into four categories related to their different deep crustal structural characteristics.Highly active ClassⅠ,ClassⅡ,and ClassⅢdepressions are predicted to be favorable prolific depressions.This study expands the research on quantitatively predicting favorable prolific depressions in the Bohai Basin using the deep crustal structure and can contribute to reducing production costs and improving exploration efficiency in future explorations.

On Performance Characteristics of a Nonparametric Subset Selection Procedure with a Small Randomized Block Experimental Design

This article addresses the issue of computing the constant required to implement a specific nonparametric subset selection procedure based on ranks of data arising in a statistical randomized block experimental design. A model of three populations and two blocks is used to compute the probability distribution of the relevant statistic, the maximum of the population rank sums minus the rank sum of the “best” population. Calculations are done for populations following a normal distribution, and for populations following a bi-uniform distribution. The least favorable configuration in these cases is shown to arise when all three populations follow identical distributions. The bi-uniform distribution leads to an asymptotic counterexample to the conjecture that the least favorable configuration, i.e., that configuration minimizing the probability of a correct selection, occurs when all populations are identically distributed. These results are consistent with other large-scale simulation studies. All relevant computational R-codes are provided in appendices.

Instructions for Authors

Applied Geophysics a ccepts original papers relating to all disciplines and a pplications of geophysics.All manuscripts are peer-reviewed by at least two referees and will not be processed further until favorable referees’reports are obtained.

Analysis of the world deepwater oil and gas exploration situation

The global trends in deepwater oil and gas exploration,characteristics of deepwater oil and gas discovery,and layout of deepwater oil and gas exploration business by seven major international oil companies are systematically analyzed using commercial databases(e.g.S&P Global and Rystad)and public information of oil companies.The deepwater area is currently the most important domain for global oil and gas exploration and discovery,with the most discoveries and reserves in passive continental margin basins.The deepwater discoveries have the greatest contribution to the total newly discovered oil and gas reserves in the sea areas,with an increasing number of lithological reservoirs discovered,and oil and gas discoveries mainly distributed in the Mesozoic–Cenozoic.The seven major international oil companies are widely active in various aspects of deepwater oil and gas exploration and development,and play a leading role.Based on years of theoretical understanding of global oil and gas geology and resource evaluation,it is proposed that favorable deepwater exploration areas in the future will mainly focus on three major areas:the Atlantic coast,the Indian Ocean periphery,and the Arctic Ocean periphery.Six suggestions are put forward for expanding overseas deepwater oil and gas exploration business:first,expand the sources for obtaining multi-user seismic data and improve the scientific selection of deepwater exploration areas;second,increase efforts to obtain deepwater exploration projects in key areas;third,adopt various methods to access into/exit from resource licenses flexibly;fourth,acquire licenses with large equity and operate in“dual-exploration”model;fifth,strengthen cooperation with leading international oil companies in deepwater technology;and sixth,improve business operation capabilities and gradually transform from“non-operators”to“operators”.

Reservoir Characteristics and Favorable Area Prediction of Chang-6 Reservoir in Zhouguan Area, Ordos Basin

Based on the analysis of a large number of core samples, logging results, logging interpretation data and dynamic data in the study area, the characteristics of Chang 6 reservoir in Zhouguan area of Baihe area are studied, and the favorable reservoir areas in the study area are predicted. The results show that the lithology of Chang 6 reservoir is mainly light gray and gray fine-grained to very fine-grained feldspar lithic sandstone. The pore types are mainly residual intergranular pores and feldspar dissolved pores, including debris dissolved pores and microfractures. The porosity and permeability values are low, which belongs to low porosity-low permeability and ultra-low permeability reservoirs. According to the reservoir distribution characteristics and related data, the Chang 6 reservoir in the study area is divided into two types, mainly Class II and Class III reservoirs. The comprehensive evaluation predicts that the spatial distribution of the favorable area of Chang 6 reservoir is not uniform, but the distribution area is large, which has broad exploration and development value, and provides the necessary conditions for the distribution study of the favorable oil-bearing zone in this area and the preparation for the next exploration and development.

Favorable lithofacies and pore characteristics of the Permian Longtan Formation shale in the southern Sichuan Basin

The marine–continental transitional shale (MCTS) reservoirs of the Longtan Formation (LTF) are widely distributed in the Sichuan Basin. However, the LTF shale exhibits considerable variations in mineral composition and pore characteristics, which makes identifying the 'sweet spot'a challenging task. To address this issue, 10 samples from four typical shale gas wells in the LTF in the southern Sichuan Basin were selected and analyzed for total organic carbon (TOC) content, whole-rock composition using X-ray diffraction (XRD), low-pressure gas adsorption, and high-pressure mercury intrusion. The lithofacies distribution and pore structure of the MCTS were studied to determine the pore structural characteristics and the primary factors influencing pore formation in different types of shale lithofacies in the LTF. The lithofacies of the LTF shale in the study area can be classified into three categories: siliceous clay shale, clay shale and mixed shale. Mineral content has a significant impact on the pore characteristics, while TOC content has a minor effect on the pore volume and specific surface area of micropores and mesopores. It can be inferred that the mesopores in the MCTS are mainly related to clay mineral pores, and mineral dissolution and TOC content are not the primary factors contributing to pore formation.

基于Flow-3D的水平射流冲刷泥沙数值模拟

为了模拟分析闸口水平射流对下游泥沙冲刷的全过程,基于水工模型试验,采用Flow-3D软件中的泥沙冲刷模型结合RNG模型和FAVOR方法,进行了水平射流冲刷固定底板下游泥沙的模拟试验。根据原水工模型条件,结合模型试验结果,对冲刷过程中的水面线、流速分布、冲坑深度和堆丘高度进行了对比分析。结果显示,数值模拟与原试验结果中水面线和流速分布吻合度很高,冲刷过程中的冲坑深度与堆丘的高度也很接近。分析了冲刷过程中的泥沙运动,模拟了不同粒径泥沙的冲刷过程并将结果进行对比,发现达到冲刷平衡阶段时冲坑深度与堆丘高度随泥沙粒径的增大而减小,该结论符合理论分析,证明该软件中泥沙模型可用于物理试验中的有关变量分析。

反应堆压力容器结构完整性评估中缺陷分布分析

缺陷分布的不确定性是影响反应堆压力容器(RPV)结构完整性的最主要因素之一,而承压热冲击(PTS)事件构成了对RPV结构完整性的最大挑战。首先讨论了材料中缺陷的一般分布规律,然后分析比较了美国橡树岭国家重点实验室(ORNL)开发的FAVOR软件(6.1版本)、承压热冲击的"鉴定准则"10CFR50.61a和ASME规范Ⅺ卷在役检查规范中所规定缺陷分布的差异情况。

基于概率性断裂力学的承压热冲击分析

承压热冲击(PTS)是制约反应堆压力容器(RPV)长周期运行的主要因素,目前大多数国家(除了美国)均采用确定性断裂力学方法(DFM)开展PTS分析。在美国,核管理当局(NRC)已经批准了基于概率性断裂力学方法(PFM)的"鉴定准则"。本文基于美国橡树岭国家重点实验室开发的FAVOR软件,对比了PTS的PFM与DFM之间一些主要差别,并通过对IAEA-TECDOC-1627报告中基准考题的计算,介绍了FAVOR软件PFM的分析方法。最后,通过分析总结国内外最新的研究成果,指出当前版本FAVOR软件(6.1版本)中所考虑模型需要完善的部分。

Prediction of Hydrothermal Copper, Gold, Silver and Lead-Zinc in Northwestern Yunnan Using Weight of Evidence Model

This paper discusses the application of the model in predicting for hydrothermal Cu, Ag, Au and Pb-Zn occurrences in northwestern Yunnan. Geochemical, lineament and lithology data were the selected recognition criteria. The mentioned criteria varied against 75 known hydrothermal occurrences; the geochemical data had a weight of （W^＋= 1. 209 7, W^- =-0. 748 1） being the maximum among the three and the rest lineament and lithology have （W^＋= 0.7424, W^-= -0.449 6）, （W^＋= 0.378 7,W^-=-0.6243） respectively. The application was successful since the predicted results covers about 70% of the known deposits and predicted unknown areas.

粘性流体晃荡与弹性结构的相互耦合作用(英文)

提出了处理粘性流体晃荡与弹性结构相互耦合作用的理论及相应数值计算方法。流体运动采用N-S方程描述,控制方程采用有限差分法离散,并由超松弛迭代法求解。液体自由表面通过流体体积法进行重构。为了考虑液舱结构变形对液体晃荡的影响,应用了FAVOR技术。由此建立了描述液体晃荡与结构相互耦合作用的水弹性理论,并由相应的计算方法进行了系列运算,以考察二维液舱不同结构刚度对液体晃荡的影响。计算结果有助于进一步理解此类问题的物理现象。

Palaeogeomorphology and its control on the development of sequence stratigraphy and depositional systems of the Early Silurian in the Tarim Basin

The Silurian in the Tarim Basin was deposited on the basement deformed by the Caledonian tectonic movements at the end of the Late Ordovician. The development and distribution of sedimentary sequences of the Early Silurian have been clearly controlled by the palaeogeomorphology of the Late Ordovician. Based on unconformity characteristics and distribution of erosion, several zones can be differentiated including a high uplifted erosion zone, a transitional slope zone and a depression zone. The central and west Tabei Uplift zones show high angular unconformity and intense erosion. The Tarim Basin in the late Ordovician shows characteristics of higher in the west, lower in the east while higher in the south, lower in the north. The Early Silurian mainly developed transgressive and highstand systems tracts on the whole, while the lowstand systems tract only developed partly below the slope break. The palaeogeomorphology controlled the elastic source supply and deposit distribution. Braided delta system and tidal flat-estuary system were deposited. The duration of uplifting of the Tazhong paleo- uplift was longer than that of the Tabei paleo-uplift, and deposition was later. This led to the lower and middle members of the Kepingtage Formation missing in that area. As a large-scale transgression occurred during the deposition period of the upper member of the Kepingtage Formation, sediment from the west of the basin was transported and deposited by tides and waves, forming tidal-marine debris systems above the uplift. Proximal alluvial fan and fan delta coarse elastic deposits developed in proximal uplift zone in the east and southeast of the basin, and braided delta put forward to the transitional zone between the edge of uplift and the sea. Large-scale tidal channel, sub-distributary channel and mouth bar of the delta front can form favorable reservoirs, and they are primary targets for oil and gas exploration. This research on sequence-depositional systems development and distribution controlled by palaeogeomorphology is significant in guiding the prediction of reservoir sandstones.

A hydrocarbon enrichment model and prediction of favorable accumulation areas in complicated superimposed basins in China

The geologic conditions of superimposed basins in China are very complicated. This is mainly shown by multi-phase structural evolution, multiple sets of source-reservoir-cap rock combinations, multiple stages of hydrocarbon generation and expulsion from source rocks, multi-cycle hydrocarbon enrichment and accumulation, and multi-phase reservoir adjustment and reconstruction. The enrichment, accumulation and distribution of hydrocarbon is mainly controlled by the source rock kitchen, paleo- anticline, regional cap rock and intensity of tectonic movement. In this paper, the T-BCMS model has been developed to predict favorable areas of hydrocarbon accumulation in complicated superimposed basins according to time and spatial relationships among five key factors. The five factors include unconformity surface representing tectonic balancing （B）, regional cap rock representing hydrocarbon protection （C）, paleo-anticline representing hydrocarbon migration and accumulation （M）, source rock kitchen representing hydrocarbon generation and expulsion （S） and geological time （T）. There are three necessary conditions to form favorable areas of hydrocarbon accumulation. First, four key factors BCMS should be strictly in the order of BCMS from top to bottom. Second, superimposition of four key factors BCMS in the same area is the most favorable for hydrocarbon accumulation. Third, vertically ordered combination and superimposition in the same area of BCMS should occur at the same geological time. The model has been used to predict the most favorable exploration areas in Ordovician in the Tarim Basin in the main hydrocarbon accumulation periods. The result shows that 95% of the discovered Ordovician hydrocarbon reservoirs are located in the predicted areas, which indicates the feasibility and reliability of the key factor matching T-BCMS model for hydrocarbon accumulation and enrichment.

The multi-factor recombination and processes superimposition model for hydrocarbon accumulation:application to the Silurian in the Tarim Basin

The multi-factor recombination and processes superimposition model for hydrocarbon accumulation is put forward in view of the hydrocarbon geological characteristics of multiple episodes of structural evolution, multiple sets of source-reservoir-seal assemblage, multiple cycles of hydrocarbon accumulation and multiple episodes of readjustment and reconstruction in the complex superimposed basins in China. It is a system including theories and methods that can help to predict favorable exploration regions. According to this model, the basic discipline for hydrocarbon generation, evolution and distribution in the superimposed basins can be summarized in multi-factor recombination, processes superimposition, multiple stages of oil filling and latest stage preservation. With the Silurian of the Tarim basin as an example, based on the reconstruction of the evolution history of the four factors （paleo-anticline, source rock, regional cap rock and kinematic equilibrium belt） controlling hydrocarbon accumulation, this model was adopted to predict favorable hydrocarbon accumulation areas and favorable exploration regions following structural destruction in three stages of oil filling, to provide guidance for further exploration ofoil and gas in the Silurian of the Tarim basin.

Multiple-Element Matching Reservoir Formation and Quantitative Prediction of Favorable Areas in Superimposed Basins

Superimposed basins in West China have experienced multi-stage tectonic events and multicycle hydrocarbon reservoir formation, and complex hydrocarbon reservoirs have been discovered widely in basins of this kind. Most of the complex hydrocarbon reservoirs are characterized by relocation, scale re-construction, component variation and phase state transformation, and their distributions are very difficult to predict. Research shows that regional caprock （C）, high-quality sedimentary facies （Deposits, D）, paleohighs （Mountain, M） and source rock （S） are four geologic elements contributing to complex hydrocarbon reservoir formation and distribution of western superimposed basins. Longitudinal sequential combinations of the four elements control the strata of hydrocarbon reservoir formation, and planar superimpositions and combinations control the range of hydrocarbon reservoir and their simultaneous joint effects in geohistory determine the time of hydrocarbon reservoir formation. Multiple-element matching reservoir formation presents a basic mode of reservoir formation in superimposed basins, and we recommend it is expressed as T-CDMS. Based on the multiple-element matching reservoir formation mode, a comprehensive reservoir formation index （Tcdms） is developed in this paper to characterize reservoir formation conditions, and a method is presented to predict reservoir formation range and probability of occurrence in superimposed basins. Through application of new theory, methods and technology, the favorable reservoir formation range and probability of occurrence in the Ordovician target zone in Tarim Basin in four different reservoir formation periods are predicted. Results show that central Tarim, Yinmaili and Lunnan are the three most favorable regions where Ordovician oil and gas fields may have formed. The coincidence of prediction results with currently discovered hydrocarbon reservoirs reaches 97 %. This reflects the effectiveness and reliability of the new theory, methods and technology.

Genetic dissection of rice appearance quality and cooked rice elongation by genome-wide association study

Appearance and cooked rice elongation are key quality traits of rice. Although some QTL for these traits have been identified, understanding of the genetic relationship between them remains limited. In the present study, large phenotypic variation was observed in 760 accessions from the 3 K Rice Genomes Project for both appearance quality and cooked rice elongation. Most component traits of appearance quality and cooked rice elongation showed significant pairwise correlations, but a low correlation was found between appearance quality and cooked rice elongation. A genome-wide association study identified 74 QTL distributed on all 12 chromosomes for grain length, grain width, length to width ratio, degree of endosperm with chalkiness, rice elongation difference, and elongation index. Thirteen regions containing QTL stably expressed in multiple environments and/or exerting pleiotropic effects on multiple traits were detected. By gene-based association analysis and haplotype analysis, 46 candidate genes, including five cloned genes, and 49 favorable alleles were identified for these 13 QTL. The effect of the candidate gene Wx on rice elongation difference was validated by a transgenic strategy. These results shed light on the genetic bases of appearance quality and cooked rice elongation and provide gene resources for improving rice quality by molecular breeding.

Evaluation Methods of Profitable Tight Oil Reservoir of Lacustrine Coquina: A Case Study of Da'anzhai Member of Jurassic in the Sichuan Basin

Based on core observation,cast and fluorescent thin sections,FESEM and ESEM,coquina in Da'anzhai Member of Jurassic in Sichuan Basin were examined systematically.Together with production data and logging evaluation,a method for lacustrine coquina evaluation based on geological theory was established up.In the article,two aspects of the study were elaborated,characteristics of favorable reservoirs,and a"five-step"evaluation method for favorable coquina reservoir.According to the correlation between porosity and production data,porosity is not effective in finding high quality coquina reservoir of this area.Whereas micro research of reservoir samples from a high productivity well revealed that sparry coquina is the best lithofacies,with the most developed micro storage space of various kinds.After the favorable reservoir was sorted out,a five-step method evaluating the coquina reservoir was worked out.Correlation ofGR value and rock types suggests that GR<30 API is an effective evaluation parameter in identifying profitable reservoir lithofacies.Meanwhile,the combination of profitable reservoir rock thickness and production data revealed that the reservoirs with the highest potentiality are those with thickness of 3-18 m.Fractures are more developed in faults,folds and structural noses in the study area.Organic acid is discharged massively before the peak of hydrocarbon generation,leading to the formation of dissolution pores in the reservoir.The evaluation of organic acid was made by using the source rock indexes.After evaluating the four factors,and compiling their distribution maps,the maps were overlapped to predict favorable reservoir zones,and 7 first class and 9 second class favorable zones of coquina were picked out.

Favorable lithofacies types and genesis of marine- continental transitional black shale: A case study of Permian Shanxi Formation in the eastern margin of Ordos Basin, NW China

Based on core description,thin section identification,X-ray diffraction analysis,scanning electron microscopy,low-temperature gas adsorption and high-pressure mercury intrusion porosimetry,the shale lithofacies of Shan23 sub-member of Permian Shanxi Formation in the east margin of Ordos Basin was systematically analyzed in this study.The Shan23 sub-member has six lithofacies,namely,low TOC clay shale(C-L),low TOC siliceous shale(S-L),medium TOC siliceous shale(S-M),medium TOC hybrid shale(M-M),high TOC siliceous shale(S-H),and high TOC clay shale(C-H).Among them,S-H is the best lithofacies,S-M and M-M are the second best.The C-L and C-H lithofacies,mainly found in the upper part of Shan23 sub-member,generally developed in tide-dominated delta facies;the S-L,S-M,S-H and M-M shales occurring in the lower part of Shan23 sub-member developed in tide-dominated estuarine bay facies.The S-H,S-M and M-M shales have good pore struc-ture and largely organic matter pores and mineral interparticle pores,including interlayer pore in clay minerals,pyrite inter-crystalline pore,and mineral dissolution pore.C-L and S-L shales have mainly mineral interparticle pores and clay mineral in-terlayer pores,and a small amount of organic matter pores,showing poorer pore structure.The C-H shale has organic mi-cro-pores and a small number of interlayer fissures of clay minerals,showing good micro-pore structure,and poor meso-pore and macro-pore structure.The formation of favorable lithofacies is jointly controlled by depositional environment and diagen-esis.Shallow bay-lagoon depositional environment is conducive to the formation of type II2 kerogen which can produce a large number of organic cellular pores.Besides,the rich biogenic silica is conducive to the preservation of primary pores and en-hances the fracability of the shale reservoir.

Delineation of Potential Mineral Resources RegionBased on Geo-anomaly Unit

The geological anomaly unit method (GAUM) is a new way to delineate and evaluate ore finding targets in line with the “geological anomaly ore finding theory”. Comprehensive ore finding information from geological, geochemical and geophysical data is used for quantitative measurement of the “ore forming geological anomaly unit” in this paper. The main procedures are shown as follows: (1) The geo anomalous events associated with gold mineralization are analyzed in Tongshi gold field; (2) The zonation in the concentrated heavy minerals and the stream sediment elements of ore forming geo anomaly are studied in detail; (3) The deep geological structural framework is deduced by means of the synthetic geological interpretation of gravity and magnetic information; (4) The ore controlling geo anomalies and ore anomalies are chosen as the variables of the favorable ore forming indexes that can be used for the quantitative delineation and evaluation of the potential ore forming regions.