本研究旨在分析阿美项目中熔结环氧(Fusion Bond Epoxy, FBE)粉末涂层的质量控制方法。首先通过对比阿美项目FBE内涂标准与国内标准,分析了FBE涂层质量控制的重要性和现有问题。接着以阿美项目为例,分析了FBE内涂工艺施工难点及质量控...本研究旨在分析阿美项目中熔结环氧(Fusion Bond Epoxy, FBE)粉末涂层的质量控制方法。首先通过对比阿美项目FBE内涂标准与国内标准,分析了FBE涂层质量控制的重要性和现有问题。接着以阿美项目为例,分析了FBE内涂工艺施工难点及质量控制要点。最后总结了该质量标准的优势和局限性,对国内外FBE内涂质量把控起到了重要的借鉴意义。展开更多
Biofilm formation plays a major role in the pathogenesis of nosocomial infections caused by Staphylococcus epidermidis ( S.epidermidis ). It has been suggested that protein encoded by the fbe (fibrinogen binding prote...Biofilm formation plays a major role in the pathogenesis of nosocomial infections caused by Staphylococcus epidermidis ( S.epidermidis ). It has been suggested that protein encoded by the fbe (fibrinogen binding protein) gene of S.epidermidis enhances bacterial adherence to medical devices and biofilm formation by binding to host fibrinogen (Fg). In this study, a 1.7 kb fbe gene fragment was amplified in 111 of 115 strains of S.epidermidis clinical isolates using PCR. Contrary to expectations, only 14 strains showed marginally increased adherence to Fg-coated polystyrene wells compared with BSA coated wells. Quantitative real-time PCR revealed no statistically significant difference in Fbe expression between Fg binding strains and Fg non-binding strains. Furthermore, in the presence of soluble Fg, S.epidermidis biofilm formation decreased in a dose-dependent manner. In contrast, the Staphylococcus aureus ( S.aureus ) strain Cowan Ⅰ and other 5 S.aureus clinical isolates showed a substantial increase in both adherence and biofilm formation in the presence of Fg. The results suggest that in S.epidermidis the fbe gene may not be associated with bacterial adherence and biofilm formation.展开更多
文摘本研究旨在分析阿美项目中熔结环氧(Fusion Bond Epoxy, FBE)粉末涂层的质量控制方法。首先通过对比阿美项目FBE内涂标准与国内标准,分析了FBE涂层质量控制的重要性和现有问题。接着以阿美项目为例,分析了FBE内涂工艺施工难点及质量控制要点。最后总结了该质量标准的优势和局限性,对国内外FBE内涂质量把控起到了重要的借鉴意义。
基金This work was supported by the Chinese National Science Foundation Grant (30170845) National High Tech Research and Development Program of China (2001AA223011) and Fudan University Innovation Grant for Graduate Students
文摘Biofilm formation plays a major role in the pathogenesis of nosocomial infections caused by Staphylococcus epidermidis ( S.epidermidis ). It has been suggested that protein encoded by the fbe (fibrinogen binding protein) gene of S.epidermidis enhances bacterial adherence to medical devices and biofilm formation by binding to host fibrinogen (Fg). In this study, a 1.7 kb fbe gene fragment was amplified in 111 of 115 strains of S.epidermidis clinical isolates using PCR. Contrary to expectations, only 14 strains showed marginally increased adherence to Fg-coated polystyrene wells compared with BSA coated wells. Quantitative real-time PCR revealed no statistically significant difference in Fbe expression between Fg binding strains and Fg non-binding strains. Furthermore, in the presence of soluble Fg, S.epidermidis biofilm formation decreased in a dose-dependent manner. In contrast, the Staphylococcus aureus ( S.aureus ) strain Cowan Ⅰ and other 5 S.aureus clinical isolates showed a substantial increase in both adherence and biofilm formation in the presence of Fg. The results suggest that in S.epidermidis the fbe gene may not be associated with bacterial adherence and biofilm formation.
