The data of an experimental study of the influence of feeding and the conditional stimulus on digestive function in birds is presented. It is stated that feed consumption is a powerful stimulator of pancreatic secreti...The data of an experimental study of the influence of feeding and the conditional stimulus on digestive function in birds is presented. It is stated that feed consumption is a powerful stimulator of pancreatic secretion in broiler chicks. The secretory response of the pancreas was the highest in the morning when secretion of pancreatic juice was 1.8 times higher, amylase activity 3.2 times, proteases activity 3.3 times, lipase activity 2.1 times higher than before feeding (basal levels). The regulation of pancreatic activity is known to be complex-reflex with conditioned-reflex component being very important since it provides 25% higher juice secretion and 42% - 74% higher enzyme secretion compared to the basal levels during few minutes after the conditional signal.展开更多
Modern aquaculture must be sustainable in terms of energy consumption, raw materials used, and environmental impact, so alternatives are needed to replace fish feed with other raw materials. Enzyme use in the agri-foo...Modern aquaculture must be sustainable in terms of energy consumption, raw materials used, and environmental impact, so alternatives are needed to replace fish feed with other raw materials. Enzyme use in the agri-food industry is based on their efficiency, safety, and protection of the environment, which aligns with the requirements of a resource-saving production system. Enzyme supplementation in fish feed can improve digestibility and absorption of both plant- and animal-derived ingredients, increasing the growth parameters of aquacultural animals. Herein we summarized the recent literature that reported the use of digestive enzymes (amylases, lipases, proteases, cellulases, and hemicellulases) and non-digestive enzymes (phytases, glucose oxidase, and lysozyme) in fish feed. In addition, we analyzed how critical steps of the pelleting process, including microencapsulation and immobilization, can interfere with enzyme activity in the final fish feed product.展开更多
Objective: Since the ban of antibiotics as growth promoting feed additives in the EU in 2006 research in alternatives has gained importance. Phytogenic feed additives represent a heterogenous class of different plant ...Objective: Since the ban of antibiotics as growth promoting feed additives in the EU in 2006 research in alternatives has gained importance. Phytogenic feed additives represent a heterogenous class of different plant derived substances that are discussed to improve the health of farm animals by direct and indirect antioxidant effects and by influencing microbial eubiosis in the gastrointestinal tract. Consequently our study aimed to investigate the influence of broccoli extract and the essential oils of tur- meric, oregano, thyme and rosemary, as selected individual additives, on intestinal and faecal microflora, on xenobiotic enzymes, and on the antioxidant system of piglets. Methods: 48 four weeks old male weaned piglets were assigned to 6 groups of 8. The piglets were housed individually in stainless steel pens with slatted floor. The control group (Con) was fed a diet without an additive for 4 weeks. The diet of group BE contained 0.15 g/kg sulforaphane in form of a broccoli extract. 535, 282, 373 and 476 mg/kg of the essential oils of turmeric (Cuo), oregano (Oo), thyme (To) and rosemary (Ro) were added to the diets of the remaining 4 groups to stan-dardise supplementation to 150 mg/kg of the oils’ key terpene compounds ar-turmerone, carvacrol, thymol and 1,8-cineole. The composition of bacterial microflora was examined by cultivating samples of jejeunal and colonic mucosa and of faeces under specific conditions. The mRNA expression of xenobiotic and antioxidant enzymes was determined by reversing transcrip- tase real time detection PCR (RT-PCR). Total antioxidant status was assayed using the Trolox Equivalent Antioxidant Capacity (TEAC), and lipid peroxidation was determined by measuring thiobarbioturic acid reactive substances (TBA- RS). Results: Compared to Con piglets all additives positively influenced weight gain and feed conversion in week 1. Over the whole trial period no significant differences in performance parameters existed between the experimental groups. Compared to group Con performance of Ro piglets was, however, slightly impaired. Com- pared to Con piglets Cuo, Oo and To increased the ratio of Lactobacilli:E. coli attached to the jejunal mucosa, whereas BE and Ro impaired this ratio slightly. In contrast in colonic mucosa Ro improved Lactobacilli:E. coli ratio. In faecal samples an improvement of Lactobacilli:E. coli ratio could be analysed for To and Ro. Ro was the only additive that reduced the incidence rate of piglets tested positive for enterotoxic E. coli (ETEC). All additives significantly increased jejunal TEAC and reduced TBA-RS. In the liver BE, Cuo, Oo and To increased TEAC in tendency and Ro significantly. Liver TBA-RS were slightly reduced by all additives compared to Con piglets. Whereas the influence of BE, To and Ro on jejunal TEAC mainly was derived from the induction of xenobiotic and antioxidant enzymes (indirect antioxidant effects), Cuo and Oo influenced TEAC by direct antioxidant effects. Discussion and Conclusions: Our results have shown: That within the labiatae oils Oo and To have the potential to improve performance slightly. That phytogenic substances have a small but not sig- nificant influence on intestinal microflora. That phytogenic feed additives up-regulate the anti- oxidant system of piglets either by direct or by indirect antioxidant effects and that they may thereby improve health status. That within the labiatae oils Oo has a high direct antioxidant potential whereas Ro potently induces xenobiotic and antioxidant enzymes. That broccoli extract is an attractive new phytogenic additive, improving antioxidant status by indirect antioxidant effects. That defined combinations of selected phytogenic substances may produce additive effects. That health promoting effects of phytogenic additives in the future should be studied systematically under the challenge with pathogenic microorganisms or food derived to-xins.展开更多
The aim of this work was to compare the efficacy of a commercially available phytogenic feed additive(PFA) and an antibiotic growth promoter, which was bacitracin methylene disalicylate(BMD), on performance, nutrient ...The aim of this work was to compare the efficacy of a commercially available phytogenic feed additive(PFA) and an antibiotic growth promoter, which was bacitracin methylene disalicylate(BMD), on performance, nutrient retention, caecal colonization of bacteria and humoral immune responses against Newcastle disease in broiler chickens challenged orally with Salmonella enteritidis and Escherichia coli.One-day-old male Cobb 400 broiler chicks(n = 120) were fed with 1) a negative control(NC) diet, which is the basal diet without any added growth promoter, 2) a positive control(PC) diet, the basal diet supplemented with BMD, 500 mg/kg and 3) a diet supplemented with PFA(150 mg/kg) for 39 days and the birds were inoculated with S. enteritidis and E. coli on d 28. Supplementation of PFA improved body weight, feed conversion ratio, retention of N and crude fiber, increased fecal moisture content and decreased digesta transit time as compared with the NC and PC groups(P <0.01). Both the PC and the PFA was found to be equally effective in controlling the surge in numbers of Salmonella and E. coli following oral inoculation of these bacteria as compared with the NC group(P <0.05) at 24 h past inoculation. Caecal content analysis on d 39 indicated lower numbers of Salmonella, E. coli and Clostridium in the PC and PFA groups as compared with the NC group(P <0.05). The number of Lactobacillus in the PFA group was higher than those in the NC and PC groups(P <0.05). Humoral immune response,measured as hemagglutination inhibition titer against Newcastle disease, was better in the PC and PFA groups compared with the NC group(P <0.05) at d 21 but the difference did not last till d 39. The heterophil to lymphocyte ratio was narrower(P <0.001) and alkaline phosphatase activity was higher(P <0.01) in the PFA group as compared with the NC and PC groups on d 39. It was concluded that the PFA, which is animal, environment and consumer friendly, may be used as an effective replacement for common in-feed antibiotics like BMD to enhance broiler performance especially when the birds are exposed to heavy infections on fields.展开更多
Dietary fiber-degrading enzyme supplementation in broilers aims at off-setting the anti-nutritive effect of non-starch polysaccharides and at promoting broiler health.Recently,we demonstrated that xylanase/glucanase a...Dietary fiber-degrading enzyme supplementation in broilers aims at off-setting the anti-nutritive effect of non-starch polysaccharides and at promoting broiler health.Recently,we demonstrated that xylanase/glucanase addition in wheat-based diet improved nutrient digestibility,arabinoxylan fermentability and broiler growth.Conversely,maize arabinoxylan was found to be recalcitrant to xylanase action.These findings suggested that enzyme-mediated improvement of nutrient digestion and carbohydrate fermentation depended on the cereal type present in the diet,and may have contributed to broiler growth.Hence,we aimed at further investigating the link between dietary enzymes and carbohydrate fermentation in broilers,by studying the impact of enzyme supplementation in cereal-based diets,to the microbial communities in the ileum and ceca of broilers.For that purpose,96 one-day-old male broilers were randomly reared in two pens and received either wheat-based or maize-based starter and grower diets.At d 20,the broilers were randomly assigned to one out of four dietary treatments.The broilers received for 8 d the wheat-based or maize-based finisher diet as such(Control treatments;WC,MC)or supplemented with a xylanase/glucanase combination(Enzyme treatments;WE,ME).At d 28,samples from the digestive tract were collected,and the ileal and cecal microbiota composition was determined by 16S ribosomal RNA gene amplicon sequencing.A similar phylogenetic(alpha)diversity was observed among the four treatments,both in the ileal and the cecal samples.Furthermore,a similar microbial composition in the ileum(beta diversity)was observed,with lactobacilli being the predominant community for all treatments.In contrast,both cereal type and enzyme supplementation were found to influence cecal communities.The type of cereal(i.e.,wheat or maize)explained 47% of the total variation in microbial composition in the ceca.Further stratifying the analysis per cereal type revealed differences in microbiota composition between WC and WE,but not between MC and ME.Furthermore,the prevalence of beneficial genera,such as Faecalibacterium and Blautia,in the ceca of broilers fed wheat-based diets coincided with arabinoxylan accumulation.These findings indicated that fermentable arabinoxylan and arabinoxylo-oligosaccharides released by dietary xylanase may play an important role in bacterial metabolism.展开更多
Despite the general use of endoxylanases in poultry feed to improve broiler performance,the abundance of different endoxylanase products and the variable response to their application in the field prevent a clear unde...Despite the general use of endoxylanases in poultry feed to improve broiler performance,the abundance of different endoxylanase products and the variable response to their application in the field prevent a clear understanding of endoxylanase functionality in vivo.To gain insight into this functionality,we investigated the impact of endoxylanase type(Belfeed from Bacillus subtilis versus Econase XT from Nonomuraea flexuosa)and dose(10,100,1,000 mg/kg)in combination with broiler age on arabinoxylan(AX)hydrolysis and fermentation in broilers(Ross 308)fed a wheat-soy based diet.In a digestibility trial and a performance trial,a total of 1,057 one-day-old chicks received the control diet or 1 of the 6 endoxylanase supplemented wheat-soy based diets with,respectively,5 replicate cages and 8 replicate pens per dietary treatment per trial.The AX content and structure,the AX digestibility values and the short-chain fatty acids produced were analysed at the level of the ileum,caeca and excreta at d 11 and 36.Endoxylanase supplementation resulted in a more extensive solubilisation of wheat AX and a reduction in the intestinal viscosity compared to the control(P<0.05).A high endoxylanase dose was,however,required to obtain increased hydrolysis of the dietary AX along the gastrointestinal tract against the control(P<0.001).Depending on the type of endoxylanase,a pool of AX with distinct physicochemical properties was created.The B.subtilis endoxylanase created a large pool of soluble AX in the ileum,thereby increasing ileal viscosity compared to broilers fed an endoxylanase from N.flexuosa(P<0.001).The N.flexuosa endoxylanase mainly triggered caecal AX fermentation in young broilers,by delivering easily fermentable AX substrates with a low degree of polymerisation(P=0.03).The effects were particularly present in young broilers(d 11).From this study,it is clear that the type and dose of endoxylanase added to wheat-soy based diets determine the nature of AX substrates formed.These,in turn,affect the intestinal viscosity and the interplay between the dietary AX compounds and microbiota,hence dictating AX digestion at young broiler ages and performance outcomes towards slaughter age.展开更多
文摘The data of an experimental study of the influence of feeding and the conditional stimulus on digestive function in birds is presented. It is stated that feed consumption is a powerful stimulator of pancreatic secretion in broiler chicks. The secretory response of the pancreas was the highest in the morning when secretion of pancreatic juice was 1.8 times higher, amylase activity 3.2 times, proteases activity 3.3 times, lipase activity 2.1 times higher than before feeding (basal levels). The regulation of pancreatic activity is known to be complex-reflex with conditioned-reflex component being very important since it provides 25% higher juice secretion and 42% - 74% higher enzyme secretion compared to the basal levels during few minutes after the conditional signal.
基金supported by the National Key Research and Development Program of China(2019YFD0900201)by the National Research Council of Italy(CNR)in the contest of the bilateral project(CNR Prot.n.0082796/2020)between CNRthe Ministry of Science and Technology of the People’s Republic of China(MOST).
文摘Modern aquaculture must be sustainable in terms of energy consumption, raw materials used, and environmental impact, so alternatives are needed to replace fish feed with other raw materials. Enzyme use in the agri-food industry is based on their efficiency, safety, and protection of the environment, which aligns with the requirements of a resource-saving production system. Enzyme supplementation in fish feed can improve digestibility and absorption of both plant- and animal-derived ingredients, increasing the growth parameters of aquacultural animals. Herein we summarized the recent literature that reported the use of digestive enzymes (amylases, lipases, proteases, cellulases, and hemicellulases) and non-digestive enzymes (phytases, glucose oxidase, and lysozyme) in fish feed. In addition, we analyzed how critical steps of the pelleting process, including microencapsulation and immobilization, can interfere with enzyme activity in the final fish feed product.
文摘Objective: Since the ban of antibiotics as growth promoting feed additives in the EU in 2006 research in alternatives has gained importance. Phytogenic feed additives represent a heterogenous class of different plant derived substances that are discussed to improve the health of farm animals by direct and indirect antioxidant effects and by influencing microbial eubiosis in the gastrointestinal tract. Consequently our study aimed to investigate the influence of broccoli extract and the essential oils of tur- meric, oregano, thyme and rosemary, as selected individual additives, on intestinal and faecal microflora, on xenobiotic enzymes, and on the antioxidant system of piglets. Methods: 48 four weeks old male weaned piglets were assigned to 6 groups of 8. The piglets were housed individually in stainless steel pens with slatted floor. The control group (Con) was fed a diet without an additive for 4 weeks. The diet of group BE contained 0.15 g/kg sulforaphane in form of a broccoli extract. 535, 282, 373 and 476 mg/kg of the essential oils of turmeric (Cuo), oregano (Oo), thyme (To) and rosemary (Ro) were added to the diets of the remaining 4 groups to stan-dardise supplementation to 150 mg/kg of the oils’ key terpene compounds ar-turmerone, carvacrol, thymol and 1,8-cineole. The composition of bacterial microflora was examined by cultivating samples of jejeunal and colonic mucosa and of faeces under specific conditions. The mRNA expression of xenobiotic and antioxidant enzymes was determined by reversing transcrip- tase real time detection PCR (RT-PCR). Total antioxidant status was assayed using the Trolox Equivalent Antioxidant Capacity (TEAC), and lipid peroxidation was determined by measuring thiobarbioturic acid reactive substances (TBA- RS). Results: Compared to Con piglets all additives positively influenced weight gain and feed conversion in week 1. Over the whole trial period no significant differences in performance parameters existed between the experimental groups. Compared to group Con performance of Ro piglets was, however, slightly impaired. Com- pared to Con piglets Cuo, Oo and To increased the ratio of Lactobacilli:E. coli attached to the jejunal mucosa, whereas BE and Ro impaired this ratio slightly. In contrast in colonic mucosa Ro improved Lactobacilli:E. coli ratio. In faecal samples an improvement of Lactobacilli:E. coli ratio could be analysed for To and Ro. Ro was the only additive that reduced the incidence rate of piglets tested positive for enterotoxic E. coli (ETEC). All additives significantly increased jejunal TEAC and reduced TBA-RS. In the liver BE, Cuo, Oo and To increased TEAC in tendency and Ro significantly. Liver TBA-RS were slightly reduced by all additives compared to Con piglets. Whereas the influence of BE, To and Ro on jejunal TEAC mainly was derived from the induction of xenobiotic and antioxidant enzymes (indirect antioxidant effects), Cuo and Oo influenced TEAC by direct antioxidant effects. Discussion and Conclusions: Our results have shown: That within the labiatae oils Oo and To have the potential to improve performance slightly. That phytogenic substances have a small but not sig- nificant influence on intestinal microflora. That phytogenic feed additives up-regulate the anti- oxidant system of piglets either by direct or by indirect antioxidant effects and that they may thereby improve health status. That within the labiatae oils Oo has a high direct antioxidant potential whereas Ro potently induces xenobiotic and antioxidant enzymes. That broccoli extract is an attractive new phytogenic additive, improving antioxidant status by indirect antioxidant effects. That defined combinations of selected phytogenic substances may produce additive effects. That health promoting effects of phytogenic additives in the future should be studied systematically under the challenge with pathogenic microorganisms or food derived to-xins.
文摘The aim of this work was to compare the efficacy of a commercially available phytogenic feed additive(PFA) and an antibiotic growth promoter, which was bacitracin methylene disalicylate(BMD), on performance, nutrient retention, caecal colonization of bacteria and humoral immune responses against Newcastle disease in broiler chickens challenged orally with Salmonella enteritidis and Escherichia coli.One-day-old male Cobb 400 broiler chicks(n = 120) were fed with 1) a negative control(NC) diet, which is the basal diet without any added growth promoter, 2) a positive control(PC) diet, the basal diet supplemented with BMD, 500 mg/kg and 3) a diet supplemented with PFA(150 mg/kg) for 39 days and the birds were inoculated with S. enteritidis and E. coli on d 28. Supplementation of PFA improved body weight, feed conversion ratio, retention of N and crude fiber, increased fecal moisture content and decreased digesta transit time as compared with the NC and PC groups(P <0.01). Both the PC and the PFA was found to be equally effective in controlling the surge in numbers of Salmonella and E. coli following oral inoculation of these bacteria as compared with the NC group(P <0.05) at 24 h past inoculation. Caecal content analysis on d 39 indicated lower numbers of Salmonella, E. coli and Clostridium in the PC and PFA groups as compared with the NC group(P <0.05). The number of Lactobacillus in the PFA group was higher than those in the NC and PC groups(P <0.05). Humoral immune response,measured as hemagglutination inhibition titer against Newcastle disease, was better in the PC and PFA groups compared with the NC group(P <0.05) at d 21 but the difference did not last till d 39. The heterophil to lymphocyte ratio was narrower(P <0.001) and alkaline phosphatase activity was higher(P <0.01) in the PFA group as compared with the NC and PC groups on d 39. It was concluded that the PFA, which is animal, environment and consumer friendly, may be used as an effective replacement for common in-feed antibiotics like BMD to enhance broiler performance especially when the birds are exposed to heavy infections on fields.
文摘Dietary fiber-degrading enzyme supplementation in broilers aims at off-setting the anti-nutritive effect of non-starch polysaccharides and at promoting broiler health.Recently,we demonstrated that xylanase/glucanase addition in wheat-based diet improved nutrient digestibility,arabinoxylan fermentability and broiler growth.Conversely,maize arabinoxylan was found to be recalcitrant to xylanase action.These findings suggested that enzyme-mediated improvement of nutrient digestion and carbohydrate fermentation depended on the cereal type present in the diet,and may have contributed to broiler growth.Hence,we aimed at further investigating the link between dietary enzymes and carbohydrate fermentation in broilers,by studying the impact of enzyme supplementation in cereal-based diets,to the microbial communities in the ileum and ceca of broilers.For that purpose,96 one-day-old male broilers were randomly reared in two pens and received either wheat-based or maize-based starter and grower diets.At d 20,the broilers were randomly assigned to one out of four dietary treatments.The broilers received for 8 d the wheat-based or maize-based finisher diet as such(Control treatments;WC,MC)or supplemented with a xylanase/glucanase combination(Enzyme treatments;WE,ME).At d 28,samples from the digestive tract were collected,and the ileal and cecal microbiota composition was determined by 16S ribosomal RNA gene amplicon sequencing.A similar phylogenetic(alpha)diversity was observed among the four treatments,both in the ileal and the cecal samples.Furthermore,a similar microbial composition in the ileum(beta diversity)was observed,with lactobacilli being the predominant community for all treatments.In contrast,both cereal type and enzyme supplementation were found to influence cecal communities.The type of cereal(i.e.,wheat or maize)explained 47% of the total variation in microbial composition in the ceca.Further stratifying the analysis per cereal type revealed differences in microbiota composition between WC and WE,but not between MC and ME.Furthermore,the prevalence of beneficial genera,such as Faecalibacterium and Blautia,in the ceca of broilers fed wheat-based diets coincided with arabinoxylan accumulation.These findings indicated that fermentable arabinoxylan and arabinoxylo-oligosaccharides released by dietary xylanase may play an important role in bacterial metabolism.
文摘Despite the general use of endoxylanases in poultry feed to improve broiler performance,the abundance of different endoxylanase products and the variable response to their application in the field prevent a clear understanding of endoxylanase functionality in vivo.To gain insight into this functionality,we investigated the impact of endoxylanase type(Belfeed from Bacillus subtilis versus Econase XT from Nonomuraea flexuosa)and dose(10,100,1,000 mg/kg)in combination with broiler age on arabinoxylan(AX)hydrolysis and fermentation in broilers(Ross 308)fed a wheat-soy based diet.In a digestibility trial and a performance trial,a total of 1,057 one-day-old chicks received the control diet or 1 of the 6 endoxylanase supplemented wheat-soy based diets with,respectively,5 replicate cages and 8 replicate pens per dietary treatment per trial.The AX content and structure,the AX digestibility values and the short-chain fatty acids produced were analysed at the level of the ileum,caeca and excreta at d 11 and 36.Endoxylanase supplementation resulted in a more extensive solubilisation of wheat AX and a reduction in the intestinal viscosity compared to the control(P<0.05).A high endoxylanase dose was,however,required to obtain increased hydrolysis of the dietary AX along the gastrointestinal tract against the control(P<0.001).Depending on the type of endoxylanase,a pool of AX with distinct physicochemical properties was created.The B.subtilis endoxylanase created a large pool of soluble AX in the ileum,thereby increasing ileal viscosity compared to broilers fed an endoxylanase from N.flexuosa(P<0.001).The N.flexuosa endoxylanase mainly triggered caecal AX fermentation in young broilers,by delivering easily fermentable AX substrates with a low degree of polymerisation(P=0.03).The effects were particularly present in young broilers(d 11).From this study,it is clear that the type and dose of endoxylanase added to wheat-soy based diets determine the nature of AX substrates formed.These,in turn,affect the intestinal viscosity and the interplay between the dietary AX compounds and microbiota,hence dictating AX digestion at young broiler ages and performance outcomes towards slaughter age.
