Influence of the combination of nutrient feed with dissolved oxygen control on taxol production in suspension cultures of Taxus chinensis (Pilg.) Rehd. was investigated in bioreactors. Addition of feeding medium with ...Influence of the combination of nutrient feed with dissolved oxygen control on taxol production in suspension cultures of Taxus chinensis (Pilg.) Rehd. was investigated in bioreactors. Addition of feeding medium with 20 g/L sucrose on day 16 enhanced both the biomass and taxol production in 5-L bioreactors. Further investigation of the fed-batch cultures in a 20-L bioreactor showed that cultivation under a low dissolved oxygen tension (DOT) of 20% for the entire culture resulted in the highest taxol content of 0.98 mg/g DW, while the taxol production was lower than that with 40% and 60% DOT. Moreover, taxol accumulation was remarkably improved by the cultivation of cells initially under DOT of 60% for 20 d followed by changing the DOT to 20% for another 12 d. By combining the use of these two strategies, a maximum taxol content of 18.7 mg/L was obtained in a 20-L bioreactor.展开更多
为早产儿尤其是极/超早产儿提供充足和均衡的营养是促进其正常生长发育和改善远期预后的物质基础,肠内营养是最佳的营养方式。既往系统回顾性分析显示使用基于循证医学的标准化喂养管理策略可有效促进全肠内喂养的建立,缩短肠外营养时间...为早产儿尤其是极/超早产儿提供充足和均衡的营养是促进其正常生长发育和改善远期预后的物质基础,肠内营养是最佳的营养方式。既往系统回顾性分析显示使用基于循证医学的标准化喂养管理策略可有效促进全肠内喂养的建立,缩短肠外营养时间,改善早产儿的营养结局,而不增加坏死性小肠结肠炎或死亡的发生风险。基于国内外相关研究,该共识制订组采用证据推荐分级的评估、制订与评价方法(Grading of Recommendations Assessment,Development and Evaluation),从早产儿肠内营养的目标、过渡期的肠内营养、稳定生长期的肠内营养、肠内特殊营养素的补充及肠内营养的监测等5个方面提出20条推荐意见,旨在为相关从业人员提供早产儿肠内营养管理的建议,以改善早产儿的临床结局。展开更多
浮萍(Lemna minor L.)是一种单子叶植物,富含蛋白质、糖类、微量元素、维生素等营养物质,以及黄酮、萜类、多糖等生物活性物质,在改善动物生长性能、免疫机能和提高产品品质等诸多方面具有积极作用,可以作为一种优质的植物蛋白源或饲料...浮萍(Lemna minor L.)是一种单子叶植物,富含蛋白质、糖类、微量元素、维生素等营养物质,以及黄酮、萜类、多糖等生物活性物质,在改善动物生长性能、免疫机能和提高产品品质等诸多方面具有积极作用,可以作为一种优质的植物蛋白源或饲料添加剂应用于动物饲料。文章就浮萍中的营养物质、生物活性物质以及在饲料中发挥的生物学功能进行归纳,以期为浮萍在饲料中的进一步开发和应用提供科学依据。展开更多
文摘Influence of the combination of nutrient feed with dissolved oxygen control on taxol production in suspension cultures of Taxus chinensis (Pilg.) Rehd. was investigated in bioreactors. Addition of feeding medium with 20 g/L sucrose on day 16 enhanced both the biomass and taxol production in 5-L bioreactors. Further investigation of the fed-batch cultures in a 20-L bioreactor showed that cultivation under a low dissolved oxygen tension (DOT) of 20% for the entire culture resulted in the highest taxol content of 0.98 mg/g DW, while the taxol production was lower than that with 40% and 60% DOT. Moreover, taxol accumulation was remarkably improved by the cultivation of cells initially under DOT of 60% for 20 d followed by changing the DOT to 20% for another 12 d. By combining the use of these two strategies, a maximum taxol content of 18.7 mg/L was obtained in a 20-L bioreactor.
文摘为早产儿尤其是极/超早产儿提供充足和均衡的营养是促进其正常生长发育和改善远期预后的物质基础,肠内营养是最佳的营养方式。既往系统回顾性分析显示使用基于循证医学的标准化喂养管理策略可有效促进全肠内喂养的建立,缩短肠外营养时间,改善早产儿的营养结局,而不增加坏死性小肠结肠炎或死亡的发生风险。基于国内外相关研究,该共识制订组采用证据推荐分级的评估、制订与评价方法(Grading of Recommendations Assessment,Development and Evaluation),从早产儿肠内营养的目标、过渡期的肠内营养、稳定生长期的肠内营养、肠内特殊营养素的补充及肠内营养的监测等5个方面提出20条推荐意见,旨在为相关从业人员提供早产儿肠内营养管理的建议,以改善早产儿的临床结局。
文摘浮萍(Lemna minor L.)是一种单子叶植物,富含蛋白质、糖类、微量元素、维生素等营养物质,以及黄酮、萜类、多糖等生物活性物质,在改善动物生长性能、免疫机能和提高产品品质等诸多方面具有积极作用,可以作为一种优质的植物蛋白源或饲料添加剂应用于动物饲料。文章就浮萍中的营养物质、生物活性物质以及在饲料中发挥的生物学功能进行归纳,以期为浮萍在饲料中的进一步开发和应用提供科学依据。