Fluorescent silk is fundamentally important for the development of future tissue engineering scaffolds.Despite great progress in the preparation of a variety of colored silks,fluorescent silk with enhanced mechanical ...Fluorescent silk is fundamentally important for the development of future tissue engineering scaffolds.Despite great progress in the preparation of a variety of colored silks,fluorescent silk with enhanced mechanical properties has yet to be explored.In this study,we report on the fabrication of intrinsically super-strong fluorescent silk by feeding Bombyx mori silkworm carbon nanodots(CNDs).The CNDs were incorporated into silk fibroin,hindering the conformation transformation,confining crystallization,and inducing orientation of mesophase.The resultant silk exhibited super-strong mechanical properties with breaking strength of 521.9±82.7 MPa and breaking elongation of 19.2±4.3%,improvements of 55.1%and 53.6%,respectively,in comparison with regular silk.The CNDs-reinforced silk displayed intrinsic blue fluorescence when exposed to 405 nm laser and exhibited no cytotoxic effect on cells,suggesting that multi-functional silks would be potentially useful in bioimaging and other applications.展开更多
Comprehensive Summary Fluorescent silk has potential application in many fields such as bioimaging,tissue engineering scaffolds,luminescent marks,and dazzling fabrics.Among the methods to endow natural silk with fluor...Comprehensive Summary Fluorescent silk has potential application in many fields such as bioimaging,tissue engineering scaffolds,luminescent marks,and dazzling fabrics.Among the methods to endow natural silk with fluorescent properties,feeding silkworms with fluorescent additives is facile,low-cost and environment friendly,which has the prospect of large-scale production.In this paper,we reviewed the research progress for this aim in the past ten years,and summarized the unified characteristics for the substances that can enter the silk gland by digestive tract of silkworms.The advantages and disadvantages of various fluorescent materials for this application are compared in detail.And the future research directions are suggested to overcome the shortcomings of the present research.展开更多
为了探究纳米颗粒添食育蚕改性蚕丝纤维的染色性能,选用2%质量分数的纳米二氧化钛和纳米氧化铜添喂家蚕,将蚕茧脱胶后进行染色并测定上染率、色深值等指标,同时探究了酸性橙Ⅱ对蚕丝纤维的染色动力学和热力学机制。结果表明:纳米颗粒添...为了探究纳米颗粒添食育蚕改性蚕丝纤维的染色性能,选用2%质量分数的纳米二氧化钛和纳米氧化铜添喂家蚕,将蚕茧脱胶后进行染色并测定上染率、色深值等指标,同时探究了酸性橙Ⅱ对蚕丝纤维的染色动力学和热力学机制。结果表明:纳米颗粒添食能改善蚕丝纤维的染色性能;酸性染料和活性染料对改性蚕丝纤维的上染率和表观色深(K S值)均高于空白组,洗涤20次后K S值仍高于空白组1.5和2.0。染色动力学和热力学分析表明酸性橙Ⅱ对各组蚕丝纤维上染过程均符合准二级吸附动力学方程和朗格缪尔(Langmuir)吸附,且属于放热过程,较低温度下更适合染色;60℃下纳米TiO 2和纳米CuO改性蚕丝纤维的实际吸附平衡饱和量分别比空白组高了0.14 g kg和1.04 g kg,计算得到染料吸附的最大值分别比空白组高了5.31 g kg和14.58 g kg,显示了改性蚕丝纤维更优的染色性能。展开更多
In Bombyx mori,two dorsolateral neurosecretory cells(NSCs)in each of thetwo brain lobes have been identified as prothoracicotropic hormone(PTTH)producingcells.This neuropeptide in insects stimulates the prothoracic gl...In Bombyx mori,two dorsolateral neurosecretory cells(NSCs)in each of thetwo brain lobes have been identified as prothoracicotropic hormone(PTTH)producingcells.This neuropeptide in insects stimulates the prothoracic gland for the synthesis andrelease of ecdysone,responsible for the molting events.Allatotropin(AT)and allatostatin(AST)are allatoregulatory neuropeptides that regulate juvenile hormone biosynthesis.Here,by using RT-qPCR,we showed that in B.mori,nutritional stress modulates themRNA expression of AT and AST-C(allatostain type C)in the central nervous systemconsisting of the brain lobes and all the associated ganglia.Using whole-mount in situhybridization,we showed that the feeding status of Bombyx larvae also influences the ex-pression of PTTH in the NSCs of the brain.Food deprivation significantly decreased themRNA expression levels of PTTH in larvae at active or terminal growth period.Further,we showed that insulin modulates the expression level of PTTH.However,its action wasdependent on the feeding status of the larvae.At feeding,the insulin decreased the PTTHexpression level,while at food deprivation,the insulin increased the PTTH expressionlevel.The data thus indicates that larval feeding status plays an important role in alteringthe mRNA expression levels of allatoregulatory peptide genes and PTTH.展开更多
基金sponsored by the National Key Research and Development Program of China(2016YFA0201700,2016YFA0201702)the Fundamental Research Funds for the Central Universities(2232019A3-06,2232019D3-02)+2 种基金the National Key Research and Development Program of China(2018YFC1105800)the National Natural Science Foundation of China(21674018,51903045)the Shanghai Belt and Road Joint Laboratory of Advanced Fiber and Low-Dimension Materials(18520750400).
文摘Fluorescent silk is fundamentally important for the development of future tissue engineering scaffolds.Despite great progress in the preparation of a variety of colored silks,fluorescent silk with enhanced mechanical properties has yet to be explored.In this study,we report on the fabrication of intrinsically super-strong fluorescent silk by feeding Bombyx mori silkworm carbon nanodots(CNDs).The CNDs were incorporated into silk fibroin,hindering the conformation transformation,confining crystallization,and inducing orientation of mesophase.The resultant silk exhibited super-strong mechanical properties with breaking strength of 521.9±82.7 MPa and breaking elongation of 19.2±4.3%,improvements of 55.1%and 53.6%,respectively,in comparison with regular silk.The CNDs-reinforced silk displayed intrinsic blue fluorescence when exposed to 405 nm laser and exhibited no cytotoxic effect on cells,suggesting that multi-functional silks would be potentially useful in bioimaging and other applications.
基金by the National Natural Science Foundation of China(21975048,21771039)the Shanghai Science and Technology Committee(19Dz2270100).
文摘Comprehensive Summary Fluorescent silk has potential application in many fields such as bioimaging,tissue engineering scaffolds,luminescent marks,and dazzling fabrics.Among the methods to endow natural silk with fluorescent properties,feeding silkworms with fluorescent additives is facile,low-cost and environment friendly,which has the prospect of large-scale production.In this paper,we reviewed the research progress for this aim in the past ten years,and summarized the unified characteristics for the substances that can enter the silk gland by digestive tract of silkworms.The advantages and disadvantages of various fluorescent materials for this application are compared in detail.And the future research directions are suggested to overcome the shortcomings of the present research.
文摘为了探究纳米颗粒添食育蚕改性蚕丝纤维的染色性能,选用2%质量分数的纳米二氧化钛和纳米氧化铜添喂家蚕,将蚕茧脱胶后进行染色并测定上染率、色深值等指标,同时探究了酸性橙Ⅱ对蚕丝纤维的染色动力学和热力学机制。结果表明:纳米颗粒添食能改善蚕丝纤维的染色性能;酸性染料和活性染料对改性蚕丝纤维的上染率和表观色深(K S值)均高于空白组,洗涤20次后K S值仍高于空白组1.5和2.0。染色动力学和热力学分析表明酸性橙Ⅱ对各组蚕丝纤维上染过程均符合准二级吸附动力学方程和朗格缪尔(Langmuir)吸附,且属于放热过程,较低温度下更适合染色;60℃下纳米TiO 2和纳米CuO改性蚕丝纤维的实际吸附平衡饱和量分别比空白组高了0.14 g kg和1.04 g kg,计算得到染料吸附的最大值分别比空白组高了5.31 g kg和14.58 g kg,显示了改性蚕丝纤维更优的染色性能。
基金the Department of Biotechnology,Government of India under Basic Research and Emerging Areas(BT/PR14289/BRB/10/837/2010,2011-14).
文摘In Bombyx mori,two dorsolateral neurosecretory cells(NSCs)in each of thetwo brain lobes have been identified as prothoracicotropic hormone(PTTH)producingcells.This neuropeptide in insects stimulates the prothoracic gland for the synthesis andrelease of ecdysone,responsible for the molting events.Allatotropin(AT)and allatostatin(AST)are allatoregulatory neuropeptides that regulate juvenile hormone biosynthesis.Here,by using RT-qPCR,we showed that in B.mori,nutritional stress modulates themRNA expression of AT and AST-C(allatostain type C)in the central nervous systemconsisting of the brain lobes and all the associated ganglia.Using whole-mount in situhybridization,we showed that the feeding status of Bombyx larvae also influences the ex-pression of PTTH in the NSCs of the brain.Food deprivation significantly decreased themRNA expression levels of PTTH in larvae at active or terminal growth period.Further,we showed that insulin modulates the expression level of PTTH.However,its action wasdependent on the feeding status of the larvae.At feeding,the insulin decreased the PTTHexpression level,while at food deprivation,the insulin increased the PTTH expressionlevel.The data thus indicates that larval feeding status plays an important role in alteringthe mRNA expression levels of allatoregulatory peptide genes and PTTH.
