Aim:The present study was conducted to investigate the protective effect of chamomile fl owers methanolic extract(CFME)and fennel seeds methanolic extract(FSME)on azathioprine(AZA),an immunosuppressant drug,which indu...Aim:The present study was conducted to investigate the protective effect of chamomile fl owers methanolic extract(CFME)and fennel seeds methanolic extract(FSME)on azathioprine(AZA),an immunosuppressant drug,which induced a liver injury and oxidative stress in rats.Methods:Rats were divided into 6 groups(8 rats each)and treated orally for 28 consecutive days as follows.Group 1:rats were given normal saline and used as controls;group 2:rats treated with CFME(200 mg/kg);group 3:rats treated with FSME(200 mg/kg);group 4:rats treated with AZA(25 mg/kg);and groups 5 and 6:rats treated with CFME(200 mg/kg)or FSME(200 mg/kg)15 min prior to AZA(25 mg/kg)treatment.At the end of experimental period,blood and liver samples were collected from all groups for biochemical analysis and histological examination.Results:The obtained data revealed that AZA-induced hepatic injury in the rats as evidenced by the signifi cant increase in serum aspartate aminotransferase,alanine aminotransferase,alkaline phosphatase,cholesterol,and direct bilirubin as well as hepatic malondialdehyde level accompanied with signifi cant decrease in reduced glutathione content and total antioxidant capacity in the liver.Moreover,body weight gain showed the signifi cant decrease and relative liver weight showed the signifi cant increase on AZA treatment.The sequential signifi cant changes in biochemical parameters were accompanied by severe histological changes in the liver tissue,including hepatocytes disorganization with pyknotic nuclei,fatty degeneration,congestion,fi brosis,and bile duct necrosis around the portal tract.The areas of hemorrhages in blood vessels and in between hepatocytes were also seen.However,the results showed the potential hepatoprotective effects of CFME and FSME against AZA-induced liver injury and oxidative stress.They succeeded in restoring the biochemical parameters and improving the histological picture of the liver.This improvement was more pronounced in the rats pretreated with FSME.Conclusion:It could be concluded that CFME and FSME have hepatoprotective potentials against AZA probably due to their antioxidant properties and radical scavenging activity.展开更多
采用顶空固相微萃取法萃取茴香籽挥发性成分,然后用GC-MS联机分析,并与水蒸汽蒸馏法获取的茴香籽精油成分进行比较。用CAR on PDMS柱萃取时,检测出26种成分,解析出占总成份95.765%的22种成份,主要成份为(E)-茴香脑(相对含量76.878%),p-...采用顶空固相微萃取法萃取茴香籽挥发性成分,然后用GC-MS联机分析,并与水蒸汽蒸馏法获取的茴香籽精油成分进行比较。用CAR on PDMS柱萃取时,检测出26种成分,解析出占总成份95.765%的22种成份,主要成份为(E)-茴香脑(相对含量76.878%),p-茴香醛(4.215%),柠檬烯(4.167%)和龙蒿脑(2.792%)。水蒸汽蒸馏法萃取茴香籽,以2.44%得油率获得精油,共检测出26个成份,解析出占总成份98.720%的21种成份,主要成份是(E)-茴香脑(相对含量84.334%),龙蒿脑(5.419%),葑酮(2.619%)和柠檬烯(2.209%)。展开更多
文摘Aim:The present study was conducted to investigate the protective effect of chamomile fl owers methanolic extract(CFME)and fennel seeds methanolic extract(FSME)on azathioprine(AZA),an immunosuppressant drug,which induced a liver injury and oxidative stress in rats.Methods:Rats were divided into 6 groups(8 rats each)and treated orally for 28 consecutive days as follows.Group 1:rats were given normal saline and used as controls;group 2:rats treated with CFME(200 mg/kg);group 3:rats treated with FSME(200 mg/kg);group 4:rats treated with AZA(25 mg/kg);and groups 5 and 6:rats treated with CFME(200 mg/kg)or FSME(200 mg/kg)15 min prior to AZA(25 mg/kg)treatment.At the end of experimental period,blood and liver samples were collected from all groups for biochemical analysis and histological examination.Results:The obtained data revealed that AZA-induced hepatic injury in the rats as evidenced by the signifi cant increase in serum aspartate aminotransferase,alanine aminotransferase,alkaline phosphatase,cholesterol,and direct bilirubin as well as hepatic malondialdehyde level accompanied with signifi cant decrease in reduced glutathione content and total antioxidant capacity in the liver.Moreover,body weight gain showed the signifi cant decrease and relative liver weight showed the signifi cant increase on AZA treatment.The sequential signifi cant changes in biochemical parameters were accompanied by severe histological changes in the liver tissue,including hepatocytes disorganization with pyknotic nuclei,fatty degeneration,congestion,fi brosis,and bile duct necrosis around the portal tract.The areas of hemorrhages in blood vessels and in between hepatocytes were also seen.However,the results showed the potential hepatoprotective effects of CFME and FSME against AZA-induced liver injury and oxidative stress.They succeeded in restoring the biochemical parameters and improving the histological picture of the liver.This improvement was more pronounced in the rats pretreated with FSME.Conclusion:It could be concluded that CFME and FSME have hepatoprotective potentials against AZA probably due to their antioxidant properties and radical scavenging activity.
文摘采用顶空固相微萃取法萃取茴香籽挥发性成分,然后用GC-MS联机分析,并与水蒸汽蒸馏法获取的茴香籽精油成分进行比较。用CAR on PDMS柱萃取时,检测出26种成分,解析出占总成份95.765%的22种成份,主要成份为(E)-茴香脑(相对含量76.878%),p-茴香醛(4.215%),柠檬烯(4.167%)和龙蒿脑(2.792%)。水蒸汽蒸馏法萃取茴香籽,以2.44%得油率获得精油,共检测出26个成份,解析出占总成份98.720%的21种成份,主要成份是(E)-茴香脑(相对含量84.334%),龙蒿脑(5.419%),葑酮(2.619%)和柠檬烯(2.209%)。
