Evaluating the Effects of Aquaculture Wastewater Irrigation with Fertilizer Reduction on Greenhouse Tomato Production,Economic Benefits and Soil Nitrogen Characteristics

The utilization of aquaculture wastewater as irrigation is an effective way to recycle and reuse water and nitrogen fertilizer resources because it contains numerous nutrients.However,it is still unclear that the pattern of substituting aquaculture wastewater irrigation for fertilizer supplementing is conducive to improving the soil nitrogen status,fruit yield and water-fertilizer use efficiency for tomato production.In this context,the experiment was intended to establish the appropriate irrigation regime of aquaculture wastewater in tomato production for freshwater replacement and fertilizer reduction to ensure good yields.Pot experiments were conducted with treatments as farmers accustomed to irrigation and fertilization used as control(CK),1.75 L aquaculture wastewater with base fertilizer(W1),2 L aquaculture wastewater with base fertilizer;and 2.25 L aquaculture wastewater with base fertilizer(W3).We examined the effects of aquaculture wastewater irrigation on soil nitrogen distribution,Nrelated hydrolases,tomato yield,and economic benefits.The results showed that the control treatment had the highest N input,about 24.68%higher than the W3 treatment,while the yield was only about 7.81%higher than W3.This indicated that the overuse of chemical fertilizer was present in the current tomato production.Although the reduction of fertilizer in aquaculture wastewater irrigation caused a decrease in tomato production,this economic loss can be compensated by cost savings in the wastewater disposal.Among aquaculture wastewater treatments,the W3 treatment had the highest overall benefit,achieving 62.63%freshwater savings,37.50%fertilizer input reduction,and an economic return of approximately 19,466 Yuan per hectare higher than the control.Additionally,increasing the irrigation volume of aquaculture wastewater could provide more available nutrients to the soil,which were more prevalent in the form of organic nitrogen.The lower soil nitrate reductase activities(NR)under aquaculture wastewater treatments after harvesting also proved that this pattern was beneficial to reduce soil nitrate nitrogen residues.Overall,the results demonstrate that aquaculture wastewater irrigation alleviates the soil nitrate residues,improves nutrient availability,and results in more economic returns with water and fertilizer conservation for the greenhouse production of tomatoes.

Effects of Nitrogen Fertilizer Reduction and Application of Nitrogen Fertilizer as Base Fertilizer on Rapeseed Yield and Nitrogen Absorption

[Objective] The aim was to research effects of N fertilizer reduction and application of N fertilizer （as base fertilizer） on rapeseed yield and N absorption. [Method] Based on Ganyouza No.5, the ratio of N, P2O5 andK2O was set at 1：0.5：0.5; N fertilizers were set involving reduced quantity at 150 kg/hm2 and preferred quantity at 180 kg/hm2; 100%, 80% and 60% of N fertilizers were applied as base fertilizers in the test respectively. In general, field tests were conducted to explore effects of reduced N fertilizer and application of N fertilizer as base fertilizer on rapeseed yield and N absorption. [Resalt] When applied N fertilizer as base fertilizer was the same, plant height, stem diameter, length of major inflorescence, number of effective branch, pod number per plant, seed number per pod, and biomass yield in group with preferred N quantity were significantly higher than those in group with reduced N fertilizer. Rapeseed yield and profits in group with preferred N quantity were signifi- cantly higher than those in group with reduced N fertilizer in field with moderate fertili- ty. In fields with higher fertility, however, the two factors were just a little higher. In group with reduced N fertilizer, use efficiency of N fertilizer, N uptake efficiency, par- tial factor productivity and harvest index of N were all significantly higher than those in group with preferred N fertilizer. Agronomic nitrogen use efficiency in group with preferred N fertilizer was significantly higher than that in group with reduced N fertiliz- er in field with moderate fertility and was significantly lower in field with high yield. With amounts of N, P and K fertilizers fixed, economic characters, yield constitution, yield, profits and N absorption in group, where 60% of N fertilizers were applied as base fertilizer, were significantly higher than those in groups with 80% or 100% of base fertilizer （N fertilizers）. These indicated that rational fertilization would maintain rapeseed yield high and reduce N input to improve use efficiency of N fertilizer. On the other hand, it is effective to improve rapeseed yield.＇to reduce N fertilizer to 150 kg/hm2, and application of 60%. of N fertilizers as base fertilizer is still proved optimal at present. [Conclusion] The research provides.theoretioal and technical references for improvement of yield of rapeseed and efficiency of N fertilizer.

Study on the Effect of Indolebutyric Acid-Lanthanum Chelate on Vegetable Growth and Its Molecular Mechanism

In order to reduce the non-point source pollution caused by the large or excessive application of chemical fertilizers and pesticides,this study was aimed to develop new alternative functional substances.The effects of different concentrations of indolebutyric acid-lanthanum chelate on seed germination and tomato seedling growth were studied by germination test of cucumber and tomato seeds and pot experiment of tomato seedlings.The results showed that the optimum concentration of indolebutyric acid-lanthanum chelate could promote the germination and growth of tomato and cucumber seeds.Among them,0.02 mg/L of indolebutyric acid-lanthanum chelate is the optimum concentration for tomato and cucumber seed germination.When the concentration is 0.1 mg/L,it is the optimum concentration for radicle and hypocotyl growth.In the pot experiment,when the optimum concentration of indolebutyric acid-lanthanum chelate on tomato seedlings was 0.5 mg/L,the plant height,stem diameter,aboveground biomass,underground biomass and leaf area of tomato seedlings could be significantly promoted.At the same time,the absorption of the main elements P,K,Mg and trace elements Fe,Mn,Cu,Zn and Na and their transfer from the underground part to the aboveground part of the seedlings were enhanced.The relative expression of the tomato expansion protein genes leEXP2,leEXP18 and leEXP5 was also significantly increased.Therefore,the suitable concentration of indolebutyric acid-lanthanum chelate has the effect of promoting root growth and promoting the quality of tomato seedlings.

Using diffusive gradients in thin films technique for in-situ measurement of labile phosphorus around Oryza sativa L. roots in flooded paddy soils

Behavior of phosphorus(P) in flooded rice soil is controlled by iron(Fe) redox cycling in root-zone. In this study, we applied a novel approach—the diffusive gradients in thin films(DGT) technique—for investigating the in-situ distribution of labile phosphorus(P) and Fe in close proximity to Asian rice(Oryza sativa L.) roots at submillimeter to millimeter spatial resolutions during the seedling and booting stages. We conducted a seven-year field experiment under rice-wheat rotation with different P fertilizer treatments. The results showed a significant and strong positive relationship of the average DGT-labile P concentration with soil Olsen P(R2= 0.77, P < 0.01) and with rice total P concentration(R^(2)= 0.62, P < 0.05). Furthermore, results on one-and two-dimensional changes of DGT-labile P indicated that fertilization only in the wheat season produced sufficient amounts of labile P in the flooded paddy soils, similar to when fertilizer was applied only in the rice season;dissolved P concentrations, however, were lower. A co-occurrence and significant positive correlation(P < 0.01) between DGT-labile P and Fe indicated Fe-coupled mobilization of P in flooded paddy soils. These results collectively indicated that the DGT technique provided information on in-situ distribution of labile P and its variability in close proximity to rice roots. This suggests that the DGT technique can improve our understanding of in-situ and high-resolution labile P processes in paddy soils and can provide useful information for optimizing P fertilization.